TMB One Solution from Promega

Product Specs
ItemTMB One Solution
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG7431
Quantity100ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Autoantibodies neutralizing type 1 interferons in two cohorts of people with HIVJournal of Human ImmunityMarch 2, 2026Julie A. Jensen, Nanna Mørk, Martin Tolstrup, Ole Søgaard, Thomas Dalhuisen, Antonio Rodriguez, Rebecca Hoh, Michael J. Peluso, Steven G. Deeks, Jean-Laurent Casanova, et al.plates were washed three times before addition of tetramethylbenzidin (TMB) one solution substrate (G7431; Promega) for 5 min. The reaction was stopped with 1M H2SO4 (1603131003; Sigma-Aldrich), and absorbance

(2) A Competition‐Based Strategy for the Isolation of an Anti‐Idiotypic Blocking Module and Fine‐Tuning for Conditional Activation of a Therapeutic AntibodyBiotechnology JournalDecember 10, 2024Jan Habermann, Dominic Happel, Adrian Bloch, Charles Shin, Harald Kolmar(Bio‐Rad: 1610381) for 1 h at RT. After five subsequent washing steps, 100 µL of TMB One solution (Promega: G7431) was added to each well, incubated until color change, and the reaction was stopped with 100 µL of a

(3) Acquisition of complement fixing antibodies targeting Plasmodium falciparum merozoites in infants and their mothers in UgandaFrontiers in ImmunologyNovember 28, 2023Susanne E. Mortazavi, Allan Lugaajju, Maria Nylander, Lena Danielsson, Muyideen Kolapo Tijani, James G. Beeson, Kristina E. M. PerssonLaboratories, USA, cat#1706515), each incubation was 1 h. TMB One Solution (Promega, Madison, WI, USA, cat#G7431) was added, the reaction stopped with 1 M H2SO4 and absorbance read at 450 nm (Multiskan Sky, ThermoFisher

(4) Deep Characterization and Comparison of Different Retrovirus-like Particles Preloaded with CRISPR/Cas9 RNPsInternational Journal of Molecular SciencesJuly 13, 2023Max Wichmann, Cecile L. Maire, Niklas Nuppenau, Moataz Habiballa, Almut Uhde, Katharina Kolbe, Tanja Schröder, Katrin Lamszus, Boris Fehse, Dawid Głów300 μL washing buffer (PBS/0.05% Tween). For Cas9 quantification, 100 μL of TMB substrate solution (G7431; Promega) were added to the wells for 20 min at 21 °C with subsequent addition of 100 μL stop solution

(5) Thin Layer Immunoassay: An Economical Approach to Diagnose Helicobacter pylori Infection in Gastroduodenal Ulcer Disease Patients of Pakistan, a Comparative AnalysisDiagnostics (Basel)January 31, 2023Faisal Aziz, Shahana Urooj KazmiPBS-T and incubated for 1 h at 37 °C. Later, 5 µL of TMB one solution (Tetramethylbenzidine) substrate (G7431, Promega, USA) was added exactly on the antigen spot and incubated for 30 min at room temperature. The

(6) Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosisPLOS Neglected Tropical DiseasesOctober 7, 2022Xiaola Guo, Shuai Wang, Junmei Zhang, Rui Li, Yongâ€™e Zhang, Zhengrong Wang, Qingming Kong, William C. Cho, Xianghong Ju, Yujuan Shen, et al.at 37°C. After washing, the reactions were developed by incubation with 100 μL of TMB One Solution (G7431, Promega, Madison, WI, USA) for 15 min in the dark. The reactions were stopped by adding 50 μL of 2

(7) The cyanobacterial lectin, microvirin-N, enhances the specificity and sensitivity of lipoarabinomannan-based TB diagnostic tests†The AnalystFebruary 21, 2021Megan van der Horst, Leshern Karamchand, Westley S. Bauer, Andrew J. M. Nel, Jonathan M. Blackburn, David W. Wrightno. 57624) and washed three times with 200 μL PBST. A 100 μL aliquot of TMB One (Promega: Cat. no. G7431) was added to each well containing beads and the plate was incubated on a shaker for 10 minutes while

(8) Anti-S1 MERS-COV IgY Specific Antibodies Decreases Lung Inflammation and Viral Antigen Positive Cells in the Human Transgenic Mouse ModelVaccinesNovember 1, 2020Abbas AT, El-Kafrawy SA, Sohrab SS, Tabll AA, Hassan AM, Iwata-Yoshikawa N, Nagata N, Azhar EI.color was developed by adding TMB 100 µL/well substrate solution (Promega, Madison, WIS, USA, Cat: G7431) and incubated for 30 min. Finally, the reaction was stopped by adding 100 µL/well of 2 M H2 SO4 . The

(9) Poly(amidoamine)-coated magnetic particles for enhanced detection ofSchistosomacirculating anodic antigen in endemic urine samplesThe AnalystJanuary 1, 2019Christine F. Markwalter, Paul L. A. M. Corstjens, Claire M. Mammoser, Garrett Camps, Govert J. van Dam, David W. WrightWorm Antigen (AWA-TCA), which contains approximately 3% w/w CAA.27 TMB One was purchased from Promega (G7431). A Cole-Palmer vortex mixer (UX-04726-01) with a modified ThermoFisher 15mm tube-holder (88880122)

(10) Evidence for histidine-rich protein 2 immune complex formation in symptomatic patients in Southern ZambiaMalaria JournalDecember 1, 2018Christine F. Markwalter, Lwiindi Mudenda, Mindy Leelawong, Danielle W. Kimmel, Armin Nourani, Saidon Mbambara, Philip E. Thuma, David W. Wrightantibodies were purchased from Abcam (ab9203, ab9206, ab30384). TMB One was purchased from Promega (G7431). 903 Protein Saver Cards were purchased from GE Healthcare Life Sciences (10534612). A Fisher Scientific

(11) Characterization of Plasmodium Lactate Dehydrogenase and Histidine-Rich Protein 2 Clearance Patterns via Rapid On-Bead Detection from a Single Dried Blood SpotThe American Journal of Tropical Medicine and HygieneMay 9, 2018Christine F. Markwalter, Lauren E. Gibson, Lwiindi Mudenda, Danielle W. Kimmel, Saidon Mbambara, Philip E. Thuma, David W. Wrightpurchased from KPL (Gaithersburg, MD) (No. 50-88-02), and TMB One was purchased from Promega (Madison, WI) (G7431). Dynabeads® MyOne™ streptavidin T1 beads were purchased from Life Technologies (Carlsbad, CA) (no.

(12) Simultaneous capture and sequential detection of two malarial biomarkers on magnetic microparticlesTalantaDecember 1, 2016Christine F Markwalter, Keersten M Ricks, Anna L Bitting, Lwiindi Mudenda, David W WrightMicrowell Phosphatase substrate was purchased from KPL (#50-88-02), and TMB One was purchased from Promega (G7431). The ELISA kit for pLDH was purchased from SD Bioline, S. Korea (05EK40), and the ELISA kit for PfHRPII

(13) Automated Device for Asynchronous Extraction of RNA, DNA, or Protein Biomarkers from Surrogate Patient SamplesSLAS TechnologyDecember 1, 2016Anna L. Bitting et al.ab30384) were purchased from Abcam (Cambridge, UK). 3,3′,5,5′-tetramethylbenzidine (TMB) One solution (#G7431) was purchased from Promega (Fitchburg, WI). Recombinant HRPII (#AGPF-55) was purchased from Immunology

(14) Simplified Paper Format for Detecting HIV Drug Resistance in Clinical Specimens by Oligonucleotide LigationPLOS ONEJanuary 11, 2016Nuttada Panpradist, Ingrid A. Beck, Michael H. Chung, James N. Kiarie, Lisa M. Frenkel, Barry R. Lutztimes with 300μl of 1X neutralization buffer, and 50μl of tetramethylbenzidine (TMB) One Solution (Promega, G7431, Madison, WI) were added to each well. After 3–5 minutes at room temperature, 50μl of 0.3M H2SO4 were

(15) Specific insulin/IGF1 hybrid receptor activation assay reveals IGF1 as a more potent ligand than insulinScientific reportsJanuary 21, 2015Slaaby R.PBS, incubated for 1 hour and washed again in 0.05% Tween-20 in PBS. 100 μl/well TMB One Solution #G7431 (Promega, CA, USA) was added and incubated for 30 minutes. 100 μl/well Stop Solution #50SS03 (Invitrogen,

(16) Development Of An In-House Enzyme-Linked Immunosorbent Assay Based On Helicobacter Pylori Sonicate Whole Cell Antigen For Diagnosis Of Gastroduodenal Ulcer Disease In Karachi, PakistanInternational Journal of Microbiology & Advanced ImmunologyOctober 21, 2013Aziz F, Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, Karachi, Pakistan, Taj Y, Kazmi SU, Department of Pathology, Dow Medical University, Dow University of Health Sciences, Karachi, Pakistan, Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, Karachi, Pakistanantibody (1:2,000) was added and incubated for 1 h at 37 ºC. Later, 100 µl of substrates (TMB One solution G7431, Promega, USA) was added and kept for 30 min at room temperature in dark place. The reaction was stopped

(17) Selenium Status Alters the Immune Response and Expulsion of Adult Heligmosomoides bakeri Worms in MiceInfection and ImmunityJuly 1, 2013Allen D. Smith, Lumei Cheung, Ethiopia Beshah, Terez Shea-Donohue, Joseph F. Urbanwere incubated for 1 h at 37°C, washed, and developed using TMB One substrate solution (catalog number G7431; Promega) that was added to each well. The color reaction was terminated by the addition of 0.1 M HCl,

(18) Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reactionAnalytical BiochemistryMarch 1, 2011Jonas W. Perez, Elizabeth A. Vargis, Patricia K. Russ, Frederick R. Haselton, David W. Wrightroom temperature, the wells were rinsed 5 times with PBS. Next, 100 μL of TMB One Solution (product #G7431, Promega), was added to each well and the enzymatic reaction was allowed to proceed for 10 minutes,

(19) Neuroprotective effects of testosterone in a naturally occurring model of neurodegeneration in the adult avian song control systemThe Journal of Comparative NeurologyDecember 1, 2010Christopher K. Thompson, Eliot A. Brenowitzthis antibody (Thompson and Brenowitz, 2008). The rabbit polyclonal anti-cleaved PARP antibody (1:800, G7431, Promega) was raised against p 85 fragment of the N-terminal for Human PARP that is only present after

(20) Neuroglobin expression in the cochlea of rat pups exposed to chronic very mild carbon monoxide (25 ppm) in air during and after the prenatal periodBrain ResearchFebruary 26, 2010Ivan A. Lopez, Dora Acuna, Yalda Shahram, Donald Mowlds, Amy M. Ngan, Tiranun Rungvivatjarus, Yeshika Sharma, John Edmond

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TMB One Solution

TMB One Solution from Promega

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Promega

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TMB One Solution from Promega

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