TMB High Sensitivity Substrate Solution from BioLegend

Product Specs
ItemTMB High Sensitivity Substrate Solution
CompanyBioLegend
Price $30.00Supplier Page View Company Product Page
Catalog Number421501
ApplicationsELISA
Quantity60 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Nanofilament immunotherapy induces potent antitumor vaccine responsesJournal for immunotherapy of cancerAugust 31, 2025Neil K, Génier S, Poisson MÈ, Douchin J, Giguère H, Daniel L, Gonzalez MF, Huang S, Trinh VQ, Tai LH, et al.times with 200 µL TBS-T, 100 µL 3,3'5,5'-Tetramethylbenzidine (TMB) substrate solution (BioLegend, 421501) was added to each well, and plates were incubated at room temperature. Reactions were stopped after

(2) Plasmacytoid dendritic cells in the intestine preferentially produce interferon lambda at homeostasis contributing to tonic localized innate immune responsesbioRxiv : the preprint server for biologyAugust 28, 2025Constant DA, Van Winkle JA, Norwood PA, Mishra G, Meyer KA, Laney ME, Madden SR, Griffith A, Rodrigues PF, Savan R, et al.antibody was diluted to 800 ng/mL, and signal was detected with high-sensitivity TMB substrate (BioLegend; 421501). Flow cytometry and magnetic bead enrichment Dissociated IECs and splenocytes were collected and

(3) A general and accessible approach to enrichment and characterisation of natural anti-Neu5Gc antibodies from human samples†RSC Chemical BiologyJuly 2, 2025Esme Hutton, Yumiko Uno, Emma Scott, Craig Robson, Martin A. Fascione, Nathalie SignoretRT. Plates were washed 3× with PBST and developed using TMB high sensitivity substrate (BioLegend, #421501). The reaction was stopped using 1 M H2SO4 and absorbance was read at 450 nm. Date were normalised by

(4) Intranasal Immunotherapy with Allergen-Loaded Extracellular Vesicles from Mast Cells Alleviates Allergic Asthma Inflammation in a Murine ModelInflammationMay 14, 2025Liao Huanjin, Peng Xia, Yin Yue, Ge Yiqing, Li Yanning, Wang Juan, Li Jia, Lin Lihui, Li Li, Xie Guogangan additional 2 h. Tetramethylbenzidine high sensitivity substrate solution (Biolegend, USA, Cat No.:421501) was utilized, and the absorbance was measured at 450 nm using a microplate reader from Molecular Devices

(5) An artificial transcription factor that activates potent interferon-γ expression in human Jurkat T CellsFront Mol MedJanuary 8, 2025Ashley King, Davis Noblitt, Olivia Sherron, Clara Kjerfve, Lydia Pless, Nicholas L. Truexthe wells (100 µL), incubated for 30 min, and washed with PBST (3 × 10 µL). TMB substrate (BioLegend, 421501) was added to the wells (50 µL), incubated for 15 min, and quenched with 100 µL of stop solution (BioLegend,

(6) Human monoclonal antibody cloning and expression with overlap extension PCR and short DNA fragmentsJournal of Immunological MethodsNovember 1, 2024Zachary Ende, Margarita Mishina, Robert C. Kauffman, Amrita Kumar, Rashmi Kumari, Paul R. Knight, Suryaprakash Sambharabuffer. 50 μL of high sensitivity TMB (3,3′, 5,5;-tetramethylbenzidine) solution (BioLegend®; Cat# 421501) was added to each well and stopped by adding 50 μL of stop solution, and subsequently read at 450 nm

(7) Extracellular metallothionein as a therapeutic target in the early progression of type 1 diabetesCell Stress and ChaperonesApril 1, 2024Clare K. Melchiorre, Matthew D. Lynes, Sadikshya Bhandari, Sheng-Chiang Su, Christian M. Potts, Amy V. Thees, Carol E. Norris, Lucy Liaw, Yu-Hua Tseng, Michael A. Lynesmin. Following a final wash step, 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (BioLegend Catalog #421501) was added, and color was allowed to develop in the dark at RT for 20 min 2N H2SO4 was added to stop

(8) Salivary IgA and vimentin differentiate in vitro SARS-CoV-2 infection: A study of 290 convalescent COVID-19 patientsMucosal ImmunologyFebruary 1, 2024Samuel Ellis, Rosie Way, Miranda Nel, Alice Burleigh, Ivan Doykov, Japhette Kembou-Ringert, Maximillian Woodall, Tereza Masonou, Katie-Marie Case, Arturo Torres Ortez, et al.PBS-T, for 1 hour at RT. Wells were washed as before and developed using TMB High Sensitivity Solution (421501, Biolegend, San Diego, CA, USA) for 10 minutes at RT. 0.2M sulphuric acid was used as a stop solution

(9) Opposing effects of acellular and whole cell pertussis vaccines on Bordetella pertussis biofilm formation, Siglec-F+ neutrophil recruitment and bacterial clearance in mouse nasal tissuesbioRxivJanuary 30, 2024Jesse M. Hall, Jessica L. Gutiérrez-Ferman, Mohamed M. Shamseldin, Myra Guo, Yash A. Gupta, Rajendar Deora, Purnima Dubey(Invitrogen Cat. 292318) HRP for 1 hr at RT. Plates were washed and developed with TMB (BioLegend, Cat. 421501). The reaction was stopped with 2N H2SO4 and plates were read at an AB450 nm. CXCL1 (R&D ref. DY453)

(10) Short Carbon Nanotube-Based Delivery of mRNA for HIV-1 VaccinesBiomoleculesJuly 7, 2023Yang Xu, Tammy Ferguson, Kazuya Masuda, Mohammad Adnan Siddiqui, Kelsi Poole Smith, Olivia Vest, Brad Brooks, Ziyou Zhou, Judy Obliosca, Xiang-Peng Kong, et al.PBS-0.05% Tween and 3 times with PBS, 75 µL/well of TMB High Sensitivity Substrate at RT (BioLegend Cat#: 421501, San Diego, CA, USA) was added and the plates were left for 10 to 15 min in the dark. Briefly, 75 µL/well

(11) Engineered Escherichia coli for the in situ secretion of therapeutic nanobodies in the gutCell host & microbeApril 12, 2023Lynch JP, González-Prieto C, Reeves AZ, Bae S, Powale U, Godbole NP, Tremblay JM, Schmidt FI, Ploegh HL, Kansra V, et al.,New England Biolabs,Cat#E5510S MTT assay,Trevigen,Cat#4890-25-K TMB substrate solution,Biolegend,Cat#421501 Amicon® Ultra 0.5 mL Centrifugal Filters,MerckMillipore,Cat# UFC5010BK Deposited data,, 16S rRNA

(12) Primary and secondary functions of HLA-E are determined by stability and conformation of the peptide-bound complexesCell ReportsJune 14, 2022Lucy C. Walters, Daniel Rozbesky, Karl Harlos, Max Quastel, Hong Sun, Sebastian Springer, Robert P. Rambo, Fiyaz Mohammed, E. Yvonne Jones, Andrew J. McMichael, et al.Netherlands",https://ccb.lumc.nl/ ELISA Coating buffer(x5),Biolegend,Cat#421701 ELISA Wash Buffer (x20),Biolegend,Cat#421501 TMB High sensitivity Substrate,Biolegend,Cat#421501 Stop Solution,Biolegend,Cat#423001 ,, Critical

(13) Hepatocyte-specific activity of TSC22D4 triggers progressive NAFLD by impairing mitochondrial functionMolecular MetabolismJune 1, 2022Gretchen Wolff et al.accuracy was assessed using a colorimetric method with tetramethyl benzidine substrate (TMB, BioLegend, #421501) and Horseradish Peroxidase (HRP, Life Technologies, #31490) as described at Rodriguez et al. [24].

(14) Single-nucleus RNA-seq2 reveals functional crosstalk between liver zonation and ploidyNature CommunicationsDecember 1, 2021M. L. Richter et al.well-plates was assessed using a colorimetric method with tetramethyl benzidine substrate (TMB, BioLegend, #421501) and 50 μg/mL of Horseradish Peroxidase (HRP, Life Technologies, #31490)125. Only when the plate alignment

(15) Convalescent plasma-mediated resolution of COVID-19 in a patient with humoral immunodeficiencyCell reports. MedicineJanuary 19, 2021Honjo K, Russell RM, Li R, Liu W, Stoltz R, Tabengwa EM, Hua Y, Prichard L, Kornbrust AN, Sterrett S, et al.Liquid Substrate",Sigma-Aldrich,Cat# T0440-1L "3,3′,5,5′-Tetramethylbenzidine (TMB)",BioLegend,Cat# 421501 Sulfuric Acid (1N),ICCA,Cat# 8300-32 SARS CoV2 His-tag,"Brouwer et al., Produced In House",PMID: 32540902 SARS

(16) Comparison of immunoglobulin E anti-thyroid peroxidase antibodies in patients with Hashimoto thyroiditis and chronic spontaneous urticariaMedicine and pharmacy reportsJanuary 1, 2021Çildağ S, Yenisey Ç, Ünübol M, Şentürk T.were washed three times with washing buffer. Subsequently, 100 µL TMB substrate (Biolegend, Cat No. 421501, CA, USA) was added to each well and the plates were incubated at least 1 hour at 37°C (depending on

(17) Rapid, direct detection of bacterial topoisomerase 1-DNA adducts by RADAR/ELISAAnalytical biochemistryNovember 1, 2020Sinha D, Kiianitsa K, Sherman DR, Maizels N.and washed as above. Signal detection was performed using TMB High Sensitivity Solution (BioLegend, #421501) according to manufacturer’s instructions. Absorbance was read at 450 and 570 nm, and the A570 reading

(18) Interrogating the recognition landscape of a conserved HIV-specific TCR reveals distinct bacterial peptide cross-reactivityeLifeJuly 27, 2020Mendoza JL, Fischer S, Gee MH, Lam LH, Brackenridge S, Powrie FM, Birnbaum M, McMichael AJ, Garcia KC, Gillespie GM.UV-peptide exchange ELISA (1:1000) "Chemical compound, drug",TMB High sensitivity Substrate,Biolegend,421501,Assay development step (2) for UV-peptide exchange ELISA (100 μL/well) "Chemical compound, drug",Stop

(19) Long-Term Delivery of an Anti-SIV Monoclonal Antibody With AAVFrontiers in ImmunologyMarch 17, 2020José M. Martinez-Navio, Sebastian P. Fuchs, Desiree E. Mendes, Eva G. Rakasz, Guangping Gao, Jeffrey D. Lifson, Ronald C. Desrosiers(Catalog# 2015-05, Santa Cruz) and developed with TMB High Sensitivity Substrate Solution (Catalog# 421501, Biolegend). The reaction was stopped by the addition of 50 µl of acidic stop solution (SouthernBiotech, Birmingham,

(20) Removal of N-Linked Glycosylation Enhances PD-L1 Detection and Predicts Anti-PD-1/PD-L1 Therapeutic EfficacyCancer cellAugust 12, 2019Lee HH, Wang YN, Xia W, Chen CH, Rau KM, Ye L, Wei Y, Chou CK, Wang SC, Yan M, et al.TMB Substrate,Cell Signaling Technology,Cat#7004 TMB High Sensitivity Substrate Solution,BioLegend,Cat#421501 STOP Solution,Cell Signaling Technology,Cat#7002 Stop Solution for TMB Substrate,BioLegend,Cat#423001 BSA

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TMB High Sensitivity Substrate Solution from BioLegend

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TMB High Sensitivity Substrate Solution from BioLegend

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