TMB CORE+ from Bio-Rad (AbD Serotec - FAB)

Product Specs
ItemTMB CORE+
CompanyBio-Rad
Price $497.00Supplier Page View Company Product Page
Catalog NumberBUF062B
Quantity500 ml
ApplicationsELISA
FormatReady To Use
TypeAccessory Reagent

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Uptake of long-chain fatty acids from the bone marrow suppresses CD8+ T-cell metabolism and function in multiple myelomaBlood advancesOctober 24, 2023Gudgeon N, Giles H, Bishop EL, Fulton-Ward T, Escribano-Gonzalez C, Munford H, James-Bott A, Foster K, Karim F, Jayawardana D, et al.streptavidin-HRP (Sigma Aldrich, catalog #E2866), and 3,3′,5,5′-tetramethylbenzidine substrate (Bio-Rad, catalog #BUF062B). Tumor necrosis factor α (TNF-α) in supernatants was measured by ELISA, using an TNF ELISA kit (Invitrogen,

(2) Transport of Designed Ankyrin Repeat Proteins through reconstituted human bronchial epithelia and protection against SARS-CoV-2Scientific ReportsApril 4, 2023Lisa Künzi, Sarah Ryter, Andreas Cornelius, Zaira Leni, Nathalie Baumlin, Matthias Salathe, Marcel Walser, Olivier Engler, Marianne GeiserPlates were washed three times with PBST, then incubated with 1-Step™ TMB-ELISA Substrate Solution (BUF062C, Biorad, Hercules, USA) for 5 min, prior to stopping the reaction with 1 M sulfuric acid. Plates were

(3) A rare human variant that disrupts GPR10 signalling causes weight gain in miceNature communicationsMarch 15, 2023Talbot F, Feetham CH, Mokrosiński J, Lawler K, Keogh JM, Henning E, Mendes de Oliveira E, Ayinampudi V, Saeed S, Bonnefond A, et al.performance chromogenic substrate, 3,3´,5,5´̵tetramethylbenzidine (TMB CORE+, Bio-Rad Laboratories, BUF062) was used to detect HRP activity. The reaction was terminated with 0.5 M H2SO4 and absorbance by the

(4) Human loss-of-function variants in the serotonin 2C receptor associated with obesity and maladaptive behaviorNature MedicineDecember 19, 2022Yang He, Bas Brouwers, Hesong Liu, Hailan Liu, Katherine Lawler, Edson Mendes de Oliveira, Dong-Kee Lee, Yongjie Yang, Aaron R. Cox, Julia M. Keogh, et al.PBS and the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB CORE+, Bio-Rad Laboratories, BUF062) was used to detect HRP activity. The reaction was stopped with 0.5 M H2SO4 and absorbance at 450 nm

(5) 1,25-Dihydroxyvitamin D3 suppresses CD4(+) T-cell effector functionality by inhibition of glycolysisImmunologyJuly 1, 2022Bishop EL, Gudgeon NH, Mackie GM, Chauss D, Roberts J, Tennant DA, Maslowski KM, Afzali B, Hewison M, Dimeloe S.(Bio-Rad, Cat# PHP050), streptavidin-HRP (Sigma-Aldrich, Cat# E2866), and TMB substrate (Bio-Rad, Cat# BUF062B). Extracellular metabolic flux analysis For analysis of the oxygen consumption rates (OCR) and extracellular

(6) Human gain-of-function MC4R variants show signaling bias and protect against obesityYearbook of Paediatric EndocrinologyOctober 23, 2020Lotta LA et al.chromogenic substrate 3,3´,5,5´̵tetramethylbenzidine (TMB CORE+, Bio-Rad Page 34 of 49 794 Laboratories, BUF062) was used to detect HRP activity. The reaction was terminated with 0.2 795 M H2SO4. Color reaction

(7) The Effect of NNK, A Tobacco Smoke Carcinogen, on the miRNA and Mismatch DNA Repair Expression Profiles in Lung and Head and Neck Squamous Cancer CellsCellsApril 21, 2020Doukas SG, Vageli DP, Lazopoulos G, Spandidos DA, Sasaki CT, Tsatsakis A.µL/well of TMB Core+ substrate solution (3,30 , 5, 50 -tetramethylbenzidine plus hydrogen peroxide) BUF062C; Bio-Rad) for 30 min at room temperature in the dark. We read the absorbance values immediately at 600

(8) Human Gain-of-Function MC4R Variants Show Signaling Bias and Protect against ObesityCellApril 18, 2019Lotta LA, Mokrosiński J, Mendes de Oliveira E, Li C, Sharp SJ, Luan J, Brouwers B, Ayinampudi V, Bowker N, Kerrison N, et al.performance chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB CORE+, Bio-Rad Laboratories, BUF062) was used to detect HRP activity. The reaction was terminated with 0.2 M H2SO4. Color reaction product

(9) Effects of soluble immunostimulants on mucosal immune responses in rainbow trout immersion-vaccinated against Yersinia ruckeriAquacultureJuly 1, 2018Jakob Skov, Jiwan K. Chettri, Rzgar M. Jaafar, Per W. Kania, Inger Dalsgaard, Kurt Buchmannfollowed by 3 washes in wash buffer (PBS with 0.1% Tween 20, pH 7.4). TMB Core+ substrate (Cat. no. BUF062C, AbD Serotec®) (100 µL well-1) was added for colour development, the reaction was stopped after 15 min PT by

(10) Evaluation of a melanocortin-4 receptor (MC4R) agonist (Setmelanotide) in MC4R deficiencyMolecular MetabolismOctober 1, 2017Tinh-Hai Collet, BÃ©atrice Dubern, Jacek Mokrosinski, Hillori Connors, Julia M. Keogh, Edson Mendes de Oliveira, Elana Henning, Christine Poitou-Bernert, Jean-Michel Oppert, Patrick Tounian, et al.performance chromogenic substrate – 3,3′,5,5′ tetramethylbenzidine (TMB CORE+, Bio-Rad Laboratories, BUF062) was used to detect HRP activity. The reaction was terminated with 0.2M H2SO4 and absorbance by the

(11) Succinate uptake by T cells suppresses their effector function via inhibition of mitochondrial glucose oxidationCell reportsJanuary 1, 0001"Gudgeon N, Munford H, Bishop EL, Hill J, Fulton-Ward T, Bending D, Roberts J, Tennant DA, Dimeloe S."(Bio-Rad, Cat# PHP050), streptavidin-HRP (Sigma Aldrich, Cat# E2866) and TMB substrate (Bio-Rad, Cat# BUF062B). Cytokine concentrations were corrected for cell count using known input cell number and measured percentage

(12) Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancerThe Journal of biological chemistryJanuary 1, 0001"Cha JH, Chan LC, Wang YN, Chu YY, Wang CH, Lee HH, Xia W, Shyu WC, Liu SP, Yao J, et al."#7076S). Finally, plates were washed three more times in PBST; 100 μl of substrate solution (Bio-Rad, #BUF062) was added to each well and incubated at room temperature (RT) and in the dark for 30 min. The reaction

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TMB CORE+ from Bio-Rad (AbD Serotec - FAB)

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TMB CORE+ from Bio-Rad (AbD Serotec - FAB)

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