ddPCR™™ Supermix for Probes from Bio-Rad

Product Specs
ItemddPCR™™ Supermix for Probes
CompanyBio-Rad
Price $502.00Supplier Page View Company Product Page
Catalog Number1863010
Concentration2X
Quantity500 rxns
ApplicationsPCR
Sample TypecDNA, genomic DNA, plasmid DNA
TypeSupermix

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) DDR1 Regulates Femoral Arterial Calcification in Lower-Extremity Artery Disease Through NF-Kappa B ActivationActa Physiol (Oxf)December 16, 2025Manovriti Thakur, Thibaut Quillard, Nico Angliker, Mark Siegrist, Yvonne Jansen, Yi Yan, Julia Wollenhaupt, Claudia Goettsch, Lars Maegdefessel, Nadia Sachs, et al.Digital PCR Master Mix (5×) (cat. no. A52490, Thermofisher) or ddPCR Supermix for Probes (cat. no. 1863010, Biorad), respectively (see table below). Details on probes and amounts of input DNA are specified in

(2) Programmable Argonaute-mediated single-nucleotide variant sequencing of cell-free DNA for multi-cancer early detectionNature CommunicationsDecember 3, 2025Chong Guo, Jiongyu Zhang, Shuo Zhang, Jeong Moon, Rui Yang, Xin Guan, Chengyu Hou, Minjie Pei, Kurt T. Schalper, Daniel Schreiber, et al.from Qiagen (Cat. 55114). The ddPCR™ Supermix for Probes was obtained from Bio-Rad Laboratories (Cat. 1863010). The hybridization buffer for ssDNA denaturation and annealing was obtained from Sigma-Aldrich (Cat.

(3) Hepatitis B virus is a stealth virus that minimizes proteomic and secretomic changes in primary human hepatocytesJournal of General VirologyNovember 7, 2025Karolína Štaflová, Kamila Clarová, Michal Doležal, Martin Hubálek, Alena Křenková, Jan Hodek, Iva Pichová, Aleš ZábranskýNucleoSpin Tissue kit (#740952, Macherey-Nagel), and ddPCR was performed using ddPCR™ Supermix for Probes (#1863010, Bio-Rad). Primer sequences used for qPCR are listed in Table S1. Quantification of secreted APOB Secreted

(4) Altered expression of autophagy-related molecules and β-catenin in different subtypes of thyroid cancer: co-localization in intranuclear cytoplasmic inclusionsEndocrine JournalNovember 4, 2025Yerkezhan Sailaubekova, Katsuya Matsuda, Yuko Akazawa, Hirokazu Kurohama, Yuki Matsuoka, Keiji Suzuki, Adiya Kerimbayeva, Hisayoshi Kondo, Van Phu Thang Nguyen, Thi Ngoc Anh Nguyen, et al.C228T and C250T mutations were also analyzed via ddPCR using ddPCR supermix for probes (catalog no.: 1863010; Bio-Rad) with the following primers: TERT F 5'-CAGCGCTGCCTGAAACTC-3' and TERT R 5'-GTCCTGCCCCTTCACCTT-3,

(5) RNA binding proteins PCBP1 and PCBP2 regulate pancreatic β cell translationMolecular MetabolismMay 30, 2025Matthew W. Haemmerle, Kirill Batmanov, Sabyasachi Sen, Matthew J. Varney, Alexander T. Utecht, Austin L. Good, Andrea V. Scota, Sarah A. Tersey, Louis R. Ghanem, Caroline C. Philpott, et al.gene. Droplet digital PCR was performed using ddPCR Supermix for Probes (Bio-Rad Laboratories, Cat#: 1863010) with the following cycling conditions: 95 °C for 10 min, 94 °C for 30 s, and 57.5 °C for 60 s for 40

(6) Wastewater surveillance reveals patterns of antibiotic resistance across the United StatesmedRxivApril 4, 2025Sooyeol Kim, Alessandro Zulli, Elana M. G. Chan, Dorothea Duong, Rebecca Y. Linfield, Caroline McCormack, Bradley J. White, Marlene K. Wolfe, Alexandria B. Boehm, Amy J. PickeringnM for all ARG targets. All ARG master mixes were prepared using ddPCR Supermix for Probes (BioRad #1863010, California, USA). Droplet generation was performed using an Automated Droplet Generator (BioRad #1864101,

(7) TET2 deficiency increases the competitive advantage of hematopoietic stem and progenitor cells through upregulation of thrombopoietin receptor signalingNat CommunMarch 10, 2025Yitong Yang, Severine Cathelin, Alex C. H. Liu, Amit Subedi, Abdula Maher, Mohsen Hosseini, Dhanoop Manikoth Ayyathan, Robert Vanner, Steven M. Chanfor each ddPCR reaction. The ddPCR reaction was prepared using the Bio-Rad ddPCR Supermix (Bio-Rad, #1863010). SNP assay primers and probes were ordered from Thermofisher (Supplementary Table 9). Plates were read

(8) Monoallelic expression can govern penetrance of inborn errors of immunityNatureJanuary 30, 2025O’Jay Stewart, Conor Gruber, Haley E. Randolph, Roosheel Patel, Meredith Ramba, Enrica Calzoni, Lei Haley Huang, Jay Levy, Sofija Buta, Angelica Lee, et al.volume, with TaqMan probes used at 40× concentration (0.5 μl), 10 μl of ddPCR Supermix added (Bio-Rad, 1863010), 5 μl of cDNA or blank template, and 4.5 μl H2O. Thirty-five microlitres of Droplet Generation Oil

(9) Novel digital droplet inverse PCR assay shows that natural clearance of hepatitis B infection is associated with fewer viral integrationsEmerg Microbes InfectJanuary 3, 2025Dong Li, Vikki Ho, Chiao-Fang Teng, Hung-Wen Tsai, Yuanyuan Liu, Sarah Bae, Harout Ajoyan, Jochen M. Wettengel, Ulrike Protzer, Brian S. Gloss, et al.of the inverted product was put in a 20 μL ddPCR reaction composed of 1 × ddPCR Supermix for Probes (1863010, Biorad), the forward and reverse primers and the probe sequences to quantify the Integrated HBV DNA

(10) Lentiviral vectors for precise expression to treat X-linked lymphoproliferative diseaseMolecular therapy. Methods & clinical developmentDecember 12, 2024Ayoub PG, Gensheimer J, Lathrop L, Juett C, Quintos J, Tam K, Reid J, Ma F, Tam C, McAuley GE, et al.(oPAP-SDC4) as a reference (Table S4). Reaction mixtures of 22 μL volume, comprising 1× ddPCR Master Mix (no. 1863010; Bio-Rad, Hercules, CA), 400 nmol/L primers, and 100 nmol/L probe for each set, 40 U DraI (R0129S; New England Biolabs

(11) CIRCLE-Seq for Interrogation of Off-Target Gene EditingJournal of Visualized ExperimentsNovember 1, 2024Jeffrey Inen, Chann Makara Han, David M. Farrell, Ganna Bilousova, Igor KogutMatrix,Corning,354277,Extracellular matrix (ECM) for culturing iPSCs ddPCR SuperMix for Probes,Bio-Rad,1863010, DG8 Cartridge Holder,Bio-Rad,1863051, DG8 Cartridges,Bio-Rad,1864008, DG8 Gaskets,Bio-Rad,1863009, "DPBS, no calcium, no magnesium

(12) Similarities and differences between brain and skin GNAQ p.R183Q driven capillary malformationsAngiogenesisNovember 1, 2024Sana Nasim, Colette Bichsel, Anna Pinto, Sanda Alexandrescu, Harry Kozakewich, Joyce Bischoff(Millipore Sigma, A8806); 4',6-diamidino-2-phenylindole - DAPI (Invitrogen, R37606); ddPCR SuperMix (BioRad, 1863010); FormaPure kit (Beckman Coulter, C16675); Normal goat serum (abcam, ab7481), Ulex europaeus agglutinin-I,

(13) Identification of cells of leukemic stem cell origin with non-canonical regenerative propertiesCell Reports MedicineApril 16, 2024Cameron G. Hollands, Allison L. Boyd, Xueli Zhao, Jennifer C. Reid, Charisa Henly, Amro ElRafie, David Boylan, Emily Broder, Olivia Kalau, Paige Johnson, et al.1000262 Spatial Transcriptomics,10X Genomics,Part No: 1000185 ddPCR™ Supermix for Probes,BioRad Laboratories,Cat#1863010 ,, Deposited data,Deposited data,Deposited data ,, scRNA seq,This Paper,GEO Accession: GSE255245 Spatial

(14) Intron retention as a productive mechanism in human MAPT: RNA species generated by retention of intron 3eBioMedicineFebruary 1, 2024Daniel Ruiz-Gabarre, Laura Vallés-Saiz, Almudena Carnero-Espejo, Isidro Ferrer, Félix Hernández, Ramon Garcia-Escudero, Jesús Ávila, Vega García-Escuderothe final reaction mix per well used was 11 μl ddPCR supermix for probes (Bio-Rad, RRID:SCR_008426, 1863010), 250 nM of TIR3 Probe labelled with FAM fluorophore, 450 nM of TIR3 Fw and Rv primers, 500 nM of TIR12

(15) Analysis of the Candidate Genes and Underlying Molecular Mechanism of P198, an RNAi-Related Dwarf and Sterile LineInternational Journal of Molecular SciencesDecember 22, 2023Shengbo Zhao, Junling Luo, Min Tang, Chi Zhang, Miaoying Song, Gang Wu, Xiaohong YanBriefly, 20 μL of ddPCR mixtures containing 1× ddPCR supermix (Bio-Rad, Hercules, CA, USA, Cat. No. 1863010), 800 nmol of each primer, 400 nmol of probe, and 1 μL of template were used to generate droplets. The

(16) Universal ddPCR-based assay for the determination of lentivirus infectious titer and lenti-modified cell vector copy numberMolecular Therapy - Methods & Clinical DevelopmentDecember 14, 2023Jennifer Kandell, Steven Milian, Richard Snyder, Uma Lakshmipathyappropriate supermix for singleplex reactions (ddPCR Supermix for Probes, no dUTP, Bio-Rad Catalog 1863010) or duplex reactions (Multiplex Supermix, Bio-Rad Catalog 12005939). PCR primers and probes (Thermo

(17) Improved piggyBac Transformation with Capped Transposase mRNA in Pest InsectsInternational Journal of Molecular SciencesOctober 13, 2023Irina Häcker, Tanja Rehling, Henrik Schlosser, Daniela Mayorga-Ch, Mara Heilig, Ying Yan, Peter A. Armbruster, Marc F. ScheteligµL CNV reactions contained 20–100 ng Ae. aegypti genomic DNA, 1x ddPCR Supermix for probes (BIO-RAD #1863010), 1x primer-probe mix target gene (FAM-labeled), 1x primer-probe mix reference gene (HEX-labeled), and

(18) Improvement of precision in recombinant Adeno-Associated Virus infectious titer assay with droplet digital PCR as an end-point measurementHuman Gene TherapyAugust 1, 2023Tam Minh Duong, James McAllister, Khalid Eldahan, Jennifer Wang, Eric Onishi, Kate Shen, Robert Schrock, Bingnan Gu, Peng Wang1:100 dilution following the plate layout (Supplementary Table S3). 2 × ddPCR Supermix for Probes (1863010; Bio-Rad) was used in the ddPCR together with 900 nM CMV-F primer, 900 nM CMV-R primer, and 62.5 nM

(19) Leukemic progenitor compartment serves as a prognostic measure of cancer stemness in patients with acute myeloid leukemiaCell Reports MedicineJuly 18, 2023Allison L. Boyd, Justin Lu, Cameron G. Hollands, Lili Alsostovar, Shiva Murali, Jennifer C. Reid, Wendy Ye, Sean Vandersluis, Paige Johnson, Amro ElRafie, et al.Genomics,Cat#1000009 i7 Multiplex Kit,10X Genomics,Cat#120262 ddPCR™ Supermix for Probes,BioRad Laboratories,Cat#1863010 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(20) Selecting Normalizers for MicroRNA RT-qPCR Expression Analysis in Murine Preimplantation Embryos and the Associated Conditioned Culture MediaJournal of Developmental BiologyApril 4, 2023David C. Hawke, Andrew J. Watson, Dean H. Bettswas mixed with 10 µL of ddPCR Supermix for probes (BioRad Laboratories, Mississauga, ON, Canada P/N: 1863010) and 1 µL of their respective TaqMan probes (Thermo Fisher Scientific, P/N: 4427975). Amplification

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