GoTaq Green Master Mix from Promega

Product Specs
ItemGoTaq Green Master Mix
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM7123
Quantity1,000 reactions
Concentration2X
Molecular Weight~66kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) The WalRK two-component system in Streptococcus pneumoniae ensures robustness of secondary wall polymer attachmentmBioAugust 12, 2026Justin J. Zik, Zeyu Fu, Morgan N. Price, Yujie Li, Qiao Yuan, Adam P. Arkin, Adam M. Deutschbauer, Josue Flores-Kim, Lok-To Shamof cpsA was PCR-mutagenized by amplifying with primers N1094 and N1095 using GoTaq DNA polymerase (M7123; Promega) and the following thermocycler settings: 95°C for 4 min; 20 cycles of 95°C for 30 s, 52°C

(2) Nascent chain folding status modulates ribosome dynamics and mRNA stability through the ribosome-associated chaperone Zuo1Mol CellAugust 6, 2026Suhee Chang, Ryan Kawalerski, Mehmet Tardu, Sophie Martin, Kristin S. Koutmou, Jeff Coller90°C for 4 min then collecting the supernatant. PCRs were performed with GoTaq DNA polymerase (Promega M7123). Cell culture Male human bronchial epithelial 16HBE14o-77 cell lines, obtained from Sigma (SCC150),

(3) Neuronal titration of Snca via enhancer disruption mitigates disease onset in a Parkinson's disease mouse modelBrainAugust 3, 2026Rachel J Boyd, A Ra Kho, Sarah A McClymont, Stacie K Loftus, Han Seok Ko, Andrew S McCallionSupplementary Fig. 1). A 25 μl reaction volume [consisting of 12.5 μl GoTaq® Green Master Mix (Promega Cat. No. M7123), 1 μl each of forward, reverse and internal primers at a concentration of 10 μM, 8.5 μl nuclease free

(4) Identification and validation of novel reference genes for bovine respiratory and lymphoid tissues using public transcriptomes and BRSV challenge modelPLoS OneJuly 21, 2026Cassandra D. Barber, Santiago Cornejo, Merrilee Thoresen, Matthew A. Scott, Amelia R. Woolumstemplate control). A commercially available classical PCR master mix (GoTaq® Green Master Mix, catalog #M7123, Promega, Madison, WI) was used according to the manufacturer’s protocol with the following conditions:

(5) Patient-derived forebrain cortical organoids reveal biphasic tau-MAP6-microtubule axis dysfunction in tauopathyAlzheimers DementJuly 7, 2026Xiaohuan Sun, Skandha Ramakrishnan, Victor C. Ogbolu, Nicholas M. Kanaan, Celeste M. Karch, Peter W. Baas, Liang Qiangas the housekeeping control. The RT‐PCR assay utilized GoTaq Green Master Mix (Promega, Catalog No.:M7123), with each reaction containing 100 ng of cDNA and 1 µL each of 10 µM forward and reverse primers. The

(6) Synaptic activity controls local exposure of an 'eat-me' signal via ANO3-ITPR1 signalingbioRxivJune 30, 2026Kunal Shroff, Molly C. O'Brien, Tyler J. Smith, Jialing Fang, Kelly S. Yu, Sonia I. Lombroso, Shengjie Feng, Chao Chen, Gregory A. Mohl, Indigo V.L. Rose, et al.in Supplementary Table 3. Genotyping PCR reactions consisted of 1x GoTaq Master Mix (Promega, Cat #M7123) supplemented with 500 nM of the FWD primer, 500 nM of the REV primer, and 2 μL of 1:100 diluted DNA

(7) A cross-vertebrate brain protein interaction map identifies conserved neural and non-neural complexesCell ReportsJune 23, 2026Vy Dang, Brittney Voigt, David Yang, Gabriel Hoogerbrugge, Muyoung Lee, Rachael M. Cox, Ophelia Papoulas, Claire D. McWhite, Raksha Pradeep, Janelle C. Leggere, et al.copy of the arhgef1bdf allele. All PCR reactions were performed using GoTaq Green Master Mix (Promega #M7123) and the following thermocycler settings: an initial denaturation at 95°C for 3 min, followed by 34

(8) D-alanine aminotransferase (Dat) promotes Staphylococcus aureus colonization fitness on human nasal respiratory epitheliumbioRxivJune 2, 2026Andrea I. Boyd, Katia A. Quintanilla, Isabel F. Escapa, Millie A. Lewis, Leah A. Kafer, Xi-Lei Zeng, Sarah E. Blutt, Carolyn B. Ibberson, Katherine P. Lemonwere colony-purified thrice and stocked as described above. PCR was performed using GoTaq (Promega, #M7123) using an insertion-specific primer (Table S5) and the upstream/buster primers [95] to confirm the presence

(9) SPIC-dependent erythrophagocytic macrophages drive granuloma formation and pathogen persistence during intracellular bacterial infectionbioRxivMay 30, 2026Aaron Fountain, Weixian Lin, Max J. Lain, Yuan Xue, Trung H. M. Pham554714 DAPI,ThermoFisher (Invitrogen),Cat# D1306 DNase I,Sigma,Cat# 10104159001 GoTaq PCR Master Mix,Promega,Cat# M7123 Liberase TL,Sigma,Cat# 05401020001 Live/Dead Fixable Blue,ThermoFisher (Invitrogen),Cat# L23105 Luna Universal Probe qPCR Master Mix

(10) Engulfment by brain macrophages in a short-lived vertebratebioRxivMay 5, 2026Rahul Nagvekar, Angela N. Pogson, Prateek R. Kalakuntla, Helena J. Barr, Azalia M. Martínez Jaimes, S.V. Perry, Emma K. Costa, Jingxun Chen, Felix Boos, Paloma Navarro Negredo, et al.adults) of the resulting lysate (unpurified) was added to 10 μL 2x GoTaq Green Master Mix (Promega, M7123), 6 μL Milli-Q water, and 0.5 μL each of primers pFL1, pRL1, and pmCF (each at 10 μM in Milli-Q water)

(11) Genetic interaction between Adgrg6 and Sox9 reveals a feedforward mechanism for postnatal spinal stabilityResearch SquareMay 4, 2026Valeria Aceves, Valeria Aceves, Cheng-Hai Zhang, Cheng-Hai Zhang, Jongkil Kim, Joshua Mathers, Andrew B. Mathers, Zhaoyang Lassar, Zhaoyang GrayI,NEB,M0303A EcoRV-HF,NEB,R3195S Ethanol,"Decon Labs, Inc.",2716 Glycerol,Sigma Aldrich,G7893-500ML GoTaq Green,Promega,M7123 Instant Ocean Sea Salt,Instant Ocean,Instant Ocean Low Melt Agarose,IBI Scientific,IB70056 Methanol,Fisher

(12) TIGAR deficiency enhances cardiac resilience through epigenetic programming of Parkin expressionJCI InsightApril 22, 2026Yan Tang, Stanislovas S. Jankauskas, Li Liu, Xujun Wang, Alus M. Xiaoli, Fajun Yang, Gaetano Santulli, Daorong Feng, Jeffrey E. Pessin12,021,629–12,021,650) produced a 1,185 bp amplicon. PCR amplification was performed using GoTaq Master Mixes 2X (M7123, Promega). Cycling conditions for amplifying longer PCR products were used as described on QIAGEN’s

(13) SPEN loss drives extra-follicular diffuse large B cell lymphoma with female-specific lethality and therapeutic vulnerabilitiesCancer DiscovApril 21, 2026Benedikt Pelzer, Cem Meydan, Isaac M Spiegel, Ioannis Karagiannidis, Min Xia, Matt Teater, Emma M Welter, Zowie E Searcy, Laura K Hilton, Darko Barisic, et al.quantification. The Promega GoTaq Green Master Mix was used for PCR reactions (Promega Corporation M7123), following the manufacturer’s instructions, in an Eppendorf Mastercycler Pro S Thermal Cycler (Eppendorf)

(14) The gamma-Tubulin Ring Complex promotes mitotic spindle integrity and acts as a microtubule minus-end cap during mitosisbioRxivApril 15, 2026Reham Aljumaah, Elizabeth A Turcotte, Sanjana Sundararajan, Vasilisa Aksenova, Alexei Arnaoutov, Mary Dassosamples were subjected to PCR with primers indicated in the Supplementary Table 2, using GoTaq (Promega, M7123). Information regarding sets of primers used in each case is also provided in Supplementary Figure 1. Preparation

(15) Increased WNT10B/FOXO6 signaling promotes cell fate transition in renal tubular cells to aggravate renal inflammation and fibrosisNature CommunicationsApril 10, 2026Jinhua Miao, Jiemei Li, Ping Meng, Xiaolong Li, Jiewu Huang, Ye Liang, Xian Ling, Xiaoxu Wang, Weiwei Shen, Shan Zhou, et al.respectively. Precipitated DNA was purified and analyzed by PCR using GoTaq Green Master Mix (Promega, cat. M7123) with specific gene primers (listed in Supplementary Table S5). JC-1 Mitochondrial Potential analysis HKC-8

(16) Genetic interaction between Adgrg6 and Sox9 reveals a feedforward mechanism for postnatal spinal stabilitybioRxivApril 8, 2026Valeria Aceves, Ziyi Xu, Cheng-Hai Zhang, Jongkil Kim, Joshua Ito, Kylie Mathers, Andrew B. Lassar, Zhaoyang Liu, Ryan S. GrayI,NEB,M0303A EcoRV-HF,NEB,R3195S Ethanol,"Decon Labs, Inc.",2716 Glycerol,Sigma Aldrich,G7893-500ML GoTaq Green,Promega,M7123 Instant Ocean Sea Salt,Instant Ocean,Instant Ocean Low Melt Agarose,IBI Scientific,IB70056 Methanol,Fisher

(17) Mechanisms of human mitochondrial leaderless mRNA translation initiationNature CommunicationsApril 4, 2026Shuangjie Shen, Yinghua Xu, Daniel L. Kober, Jinfan WangFollowing extraction, 1 µL of genomic DNA was added to a standard 24 µL 2× GoTaq Green PCR (Promega, #M7123), typically ~35 cycles. Sequences for screening oligos are available in Supplementary Table 1. Following

(18) Efficient genetic perturbation of murine sensory neurons in vivo using CRISPR/Cas9The Journal of PainApril 1, 2026Guadalupe García, Jacob B. Shapiro, Zachary T. CampbellOneC,Thermo Scientific, RNA Clean & Concentrator-5,Zymo Research,R1014 GoTaq Green Master Mix,Promega,M7123 HiScribe T7 High Yield RNA Synthesis Kit,New England Biolabs,E2040L "DNase I, RNase-free",Thermo Scientific,EN0521 Quick CIP

(19) Genetic Modification of Mice Using Prime EditingCurrent ProtocolsMarch 11, 2026Amr R. Salem, Xiaoling Xie, Susan H. Griffin, Lin Gan, Joseph M Miano(United States Biological, cat. no. T8655) Proteinase K (NEB, cat. no. P8107S) GoTaq Green master mix (Promega, cat. no. M7123) H2O, nuclease-free Agarose, type I, low EEO (Sigma-Aldrich, cat. no. A6013-500G) 10× TBE liquid concentrate

(20) Mapping the Mutational Landscape for Streptokinase Binding to PlasminogenbioRxivFebruary 4, 2026Srishti Baid, Matthew L Holding, Taylor Laurin, David Seimeiniak, David Ginsburg, Laura M Haynesmodified pAY-FE vector described above. The PCR products were further amplified with GoTaq (Product# M7123, Promega) using the same primers described above and gel purified using the Qiagen Gel Purification

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7/17/2020Efficient Amplification of DNA Using GoTaq® Green Master Mix Xiaoguang LiI recommend this because it saves a lot of time, and works well. I didn't recycle the gel product.