GoTaq Green Master Mix from Promega

Product Specs
ItemGoTaq Green Master Mix
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM7122
Quantity100 reactions
Concentration2X

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Development of a gene-editing strategy to overcome genetic intractability in Lactobacillus johnsoniiJournal of BacteriologyJuly 23, 2026Keerthikka Ravi, Nicole R. Falkowski, Gary B. Huffnagleas the cloning intermediate. Colony PCR screening was done using Promega GoTaq Master Mix (Promega, M7122). Plasmid DNA was purified using the E.Z.N.A. Plasmid DNA Mini Kit (Omega Bio-tek Inc, D6942-01) or

(2) Component A2 is a redox-sensitive archaeal ATPase activated by methyl-coenzyme M reductaseCurr BiolJuly 20, 2026Sophia A. Adler, Dipti D. NayakSigma,Cat#T1395 Hydroxocobalamin hydrochloride (Vitamin B12a),Millipore Sigma,Cat#H7126 GoTaq Green Master Mix,Promega,Cat#M7122 Phusion High-Fidelity DNA Polymerase,New England Biolabs,Cat#M0530S Malachite Green,Echelon Biosciences,Cat#K1501 HEPES,Fisher Bioreagents

(3) Germline-mediated ubiquitous recombination in ScxCre male mice: Implications for tendon researchPLoS OneJuly 13, 2026Haiyin Li, Christopher L. Mendias, Chike Caotail tips of P0-P4 pups and PCR genotyping was performed using GoTag® Master Mix (Promega, Cat. No. M7122). The PCR amplification program consisted of an initial denaturation at 94 ºC for 3 minutes, followed

(4) Molecular Variation in Porcine Circovirus Type 2 in Jalisco, Mexico, and Its Potential Impact on Vaccine EfficacyVaccines (Basel)June 26, 2026Alberto Jorge Galindo-Barboza, José Francisco Rivera-Benítez, Jazmín De la Luz-Armendáriz, José Iván Sánchez-Betancourt, Jesús Hernández, Alexel Jesús Burgara-Estrella, Suzel Guadalupe Sauceda-Cerecer, Laura Márquez-Valdelamar, Jaime Enrique De Alba-Camposbp fragment, together with the GoTaq Green® PCR Kit (Promega Corporation, Madison, WI, USA, Cat. No. M7122). Each 12.5 µL reaction contained 6.25 µL of Master Mix, 2 µL of primer mix, 2.5 µL of DNA template,

(5) Atg23 interacts with both the N- and C-termini of Atg9 via a hydrophobic binding pocketJournal of Biological ChemistryJune 12, 2026Zhanibek Bekkhozhin, Kelsie A. Leary, Michael J. RagusaSalI-HF, NcoI-HF, NotI-HF, DpnI (NEB R3136L, R3138S, R3193S, R3189S, R0176S); 2× GoTaq master mix (Promega M7122), NEB5 alpha (NEB C2987H), Rosetta2 (DE3) (Novagen 71397), QiaQuick PCR Purification Kit (Qiagen 28104),

(6) Noninvasive optogenetic induction of cardiac arrhythmias alters systemic hemodynamics in miceSci AdvJune 5, 2026Marcello Magri Amaral, Abigail Matt, Kaelyn H. Schloss, Fei Wang, Elena Gracheva, Yuxuan Wang, Hongwu Liang, Annie Bice, Jimin Ding, Attila Kovacs, et al.5′-TTACGGCGCTAAGGATGACT-3′ and 5′-GTGTGGAATCCGTCGAAAGT-3′. DNA amplification was performed with GoTaq Green Master Mix (M7122, Promega), according to the manufacturer’s instructions. Heart slicing and staining Dissected mouse

(7) The use of an avian macrophage-like cell line (HD11) transduced with recombinant lentiviruses to dissect the immunomodulatory role of Marek’s disease virus gene productsVirus GenesJune 1, 2026Sonsiray Alvarez-Narvaez, Steven J. Conrad, Taejoong Kim, Stephen Spatz, John R. Dunnpromoters. The PCR master mix consisted of 25 μL of the 2 × GoTaq® Green Master Mix (Promega, catalog no. M7122), 5 μL of 10 μM forward primer (IDT), 5 μL of 10 μM reverse primer (IDT), 5 μL of plasmid DNA, and 10

(8) A BRRF1-CCR4-NOT axis underlies conserved transcriptome-wide loss of splicing fidelity during gammaherpesvirus reactivationbioRxivMay 26, 2026Trang T Nguyen, Anandita Ghosh, Nadeeshika Wickramarachchige-Dona, Tina M O’Grady, Claire Roberts, Eman Ishaq, Jordan Bass, Meggie Lam, Melody Baddoo, Nathan A Ungerleider, et al.1:10 in ddH2O. PCR reactions were carried out using 10 μL of GoTaq Green Mastermix (Promega, cat. no. M7122), 2 μL of diluted cDNA, 0.5 μL of 10μM primer mix, and 7.5 μL of ddH2O (for a final reaction volume

(9) A CURE for synthetic regulation of gene expression: rapid screening of guide RNA efficacy as a framework for enabling undergraduate research in plant synthetic biologySynthetic BiologyMay 23, 2026Tawni Bull, Liz Carlsen, Noah Hoglund, Josh Blarr, Makenzie Ciernia, Hannah Daughtrey, Keana Gulnac, Zane Kathan, Ben Labovitz, Ryan Lonergan, et al.Proper removal of the genomic DNA was confirmed with PCR using GoTaq® Green Master Mix (Promega Cat No. M7122) and primers spanning the first and second intron of GID1a (Table S5). Concentrations of GID1a, GID1b,

(10) Overexpression of small-conductance Ca2+-activated K+ channel 2 attenuates pain-like behavior in female mice with cystitisJCI InsightMay 22, 2026Guadalupe Manrique-Maldonado, Xuejiao Sun, Allison L. Marciszyn, Nicolas Montalbetti, Marcelo D. Carattinomethod (77). PCR reactions included 1 μL of sample, 10 μL of 2× GoTaq Green Master Mix (Promega, catalog M7122), 1 μL of DMSO, and primers at a final concentration of 500 nM in a total reaction volume of 20 μL.

(11) Serendipitous detection of invasive malaria vector Anopheles stephensi in Kisumu, Kenya in June 2022Scientific ReportsMay 4, 2026Bryson Alberto Ndenga, Kevin Omondi Owuor, Sammy Wambua, Brian Bartilol, Marta Maia, Joseph Mwangangi, Rodney Omukuti, Salome Chemutai, Daniel Arabu, Irene Miringu, et al.al.48, respectively, were used. The PCR mixture consisted of the 5 µL of 2X GoTaq master mix (cat. M7122, Promega, Wisconsin, US), 0.5µL of primers, 0.5 µ nuclease free water and 1.5 µL of the sample. The

(12) Genetic analysis of bovine papular stomatitis and pseudocowpox viruses circulating in Hokkaido, Japan’s largest cattle-producing regionJournal of Veterinary Medical ScienceMay 1, 2026Ayaka YOSHINO, Ayaka OKADA, Yasuo INOSHIMArespectively. Each PCR reaction (10 µL) contained 1 µM each primer, 2× GoTaq Hot Start Green Master Mix (M7122, Promega, Madison, WI, USA), 5–40 ng of template DNA, and nuclease-free water. Thermal cycling conditions

(13) Deciphering tRNA repertoires and translation coordination during mouse early embryogenesis by ORACLE-tRNAseqNature CommunicationsApril 30, 2026Yun Li, Xin Wang, Ruofan Huang, Haoquan Yu, You Wu, Ze Shu, Wenlin Jiang, Zheng Cao, Zongyu Yu, Hongyan Li, et al.Genomic DNA Kit (Transgene, EE101-11), followed by PCR amplification with GoTaq® Master Mix (Promega, M7122) under the following conditions: 98 °C for 2 min; 30 cycles of 98 °C for 30 s, 59 °C for 30 s, and 72

(14) Germline-mediated ubiquitous recombination in ScxCre male mice: implications for tendon researchbioRxivApril 24, 2026Haiyin Li, Chike Caoextracted from ear punches and PCR genotyping was performed using GoTagR Master Mixes (Promega, Cat#: M7122). The PCR amplification program consisted of an initial denaturation at 94°C for 3 minutes, followed

(15) Bioremediation Potential of Leaf Endophytic Fungi in Allium ampeloprasum and Brassica oleracea var. capitataJ Fungi (Basel)April 20, 2026Dayani Pavalakumar, Sagarika Kannangara, Nadeema Dharmasiri, Chamani Amarasekara, Lanka Undugoda, Kasun M. Thambugala, Jayantha Munasinghe, Sukanya Haituk, Ratchadawan Cheewangkoon(5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TCCTCCGCTTATTGATATGC-3′) primers, 12.5 µL of GoTaq Green Master Mix (M7122, Promega, Madison, WI, USA), and 8.5 µL of nuclease-free water. Amplification was carried out using

(16) Fertilization with Protamine-2 deficient sperm triggers abnormal pronucleus development and zygotic cleavage†Biology of ReproductionApril 13, 2026Shannon R Rainsford, Kevin Tse, Benjamin William Walters, Jong-Nam Oh, Kaelyn Sumigray, Bluma J Lesch, Zachary D Smith(Supplemental Figure 1B). Polymerase chain reaction was performed using a GoTaq master mix (Promega M7122) and samples were run on a 1.5% agarose gel and visualized using a Syngene G:Box imager. Sperm preparation Adult

(17) Exploring the Association of Genetic Determinants of SIRT1, MTHFR, and MIR146A Gene Polymorphism with the Ischemic Stroke Predisposition: A Case Control StudyCellular and Molecular NeurobiologyApril 9, 2026Abdullah Hamadi, Rashid Mir, Osama M. Al-Amer, Mohammed Alasseiri, Mamdoh S. Moawadh, Jameel Barnawi, Mohammad A. Alanazi, Atif Abdulwahab A. Oyouni, Abeer Al-Tuwaijri, Fawzyah Obeedallah Albaldi, et al.primer, FI–0.12uL, RI–0.12uL of 25 pmol of each primers and 6 µL from GoTaq® Green Master Mix (cat no M7122) (Promega, USA). The optimum temperature was found to be 60 °C for SIRT1 rs7069102 C > G, 55 °C for

(18) Lipid droplet-induced T cell death sustains autoimmune tissue inflammationCell MetabolismApril 7, 2026Jitendra Kumar, Yoshinori Takashima, Jose Morales, Brandon Wong, Lina Werner, Isabel N. Goronzy, Selene Rubino, Robert T. Trousdale, Gerald J. Berry, Jörg J. Goronzy, et al.proteins","Chemicals, peptides, and recombinant proteins" All-in-One Optimized PCR Master mix,Promega,# M7122 BODIPY™ 493/503,ThermoFisher,# D3922 BODIPY™ 581/591 C11 (Lipid Peroxidation Sensor),ThermoFisher,#D3861 "Bovine Serum Albumin (BSA

(19) Variant-to-gene mapping identifies ARHGEF12 as a primary open-angle glaucoma effector gene operating within retinal ganglion cellsbioRxivMarch 30, 2026Vrathasha Vrathasha, Matthew C. Pahl, James A. Pippin, Sergei Nikonov, Jie He, Mina Halimitabrizi, Laxmi Moksha, Rebecca Salowe, Amy-Ann Edziah, Yuki Bradford, et al.and Qubit 2.0 fluorometer. PCR was performed using the GoTag Green Master Mix (Promega, catalog #: M7122, Madison, WI, USA), and the primer sets are listed in Table S8. PCR products were purified using Gel

(20) AAV2-mediated intravitreal delivery of exon-specific U1 snRNA rescues optic neuropathy in familial dysautonomiaMolecular Therapy Nucleic AcidsMarch 12, 2026Anil Chekuri, Krishnakanth Kondabolu, Emily G. Kirchner, Swanand Koli, Matthew Chagnon, Drenushe Krasniqi-Vanmeter, Max E. Stern, Jessica Bolduc, Giulia Romano, Franco Pagani, et al.starting RNA equivalent cDNA was subjected to a PCR reaction with the GoTaq green master mix (Promega, #M7122) and 30 amplification cycles (94°C for 30 s, 58°C for 30 s, and 72°C for 30 s). The human-specific forward

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(1)

7/17/2020Efficient Amplification of DNA Using GoTaq® Green Master Mix Xiaoguang LiI recommend this because it saves a lot of time, and works well. I didn't recycle the gel product.
(1)

7/23/2019Promega's PCR Master Mix for Colony PCR Works Well Atul SinghBest kit I have used so far for colony PCR.
(1)

1/28/2019Very Easy and Efficient Taq Mastermix Virginia FolgadoI needed a cheap and efficient PCR mix since I have to do a lot of PCR from bacteria colonies to check presence/absence of my target insert in the plasmid. I found this kit very easy to use; I didn't ... Read Review
(1)

8/15/2017Great PCR Master Mix Chris C We use this PCR master mix for genotyping PCR reaction for transgenic mice. We selected this product because of its ease of use, just add primers, water and template. It works well for our PCR ... Read Review
(1)

12/29/2016Super Easy PCR Premix Zeji DuGoTaq Green Master Mix is a premixed ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by ... Read Review
(1)

10/14/2015Premixed and Ready-To-Use Solution for DNA Synthesis Sunita MeenaGoTaq® Green Master Mix was mixed with primers which are specific for the gene of interest present in cDNA and kept in PCR machine for amplification. The PCR product was directly visualized in ... Read Review