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PCR Master Mixes
GoTaq Green Master Mix
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GoTaq Green Master Mix
GoTaq Green Master Mix
GoTaq Green Master Mix from Promega
(6)
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Citations:
(233)
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Promega for
GoTaq Green Master Mix
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Description
New formulation of Taq DNA Polymerase with enhanced performance over standard Taq DNA Polymerase. Available in two all-in-one master mixes: green and colorless. The green master mix allows direct gel analysis after amplification
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
GoTaq Green Master Mix
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
M7122
Quantity
100 reactions
Concentration
2X
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (233)
(1)
Protocol for detecting Helicobacter suis infection in gastric biopsies and serum by PCR and ELISA
STAR Protocols
December 1, 2023
Hidenori Matsui, Masato Suzuki, Sae Aoki, Keigo Shibayama, Kengo Tokunaga, Hidekazu Suzuki, Katsuhiro Mabe, Tsuyoshi Kenri, Emiko Rimbara
μg/mL),
Sigma-Aldrich
,Cat#
A2942
Linezolid
,
Abcam
,Cat#
ab141991
THUNDERBIRD Probe
qPCR
Mix,Toyobo,Cat#QPS-101T
GoTaq Green Master Mix
,
Promega
,Cat#
M7122
QIAquick PCR Purification Kit,
QIAGEN
,Cat#
28104
,, Deposited data,Deposited data,Deposited data ,, Whole-genome
(2)
Mice with FVB-derived sequence on chromosome 17 succumb to disseminated virus infection due to aberrant NK cell and T cell responses
iScience
November 17, 2023
Taylor N. Tibbs, Lauren J. Donoghue, Ashlyn A. Buzzelli, Ichiro Misumi, Maggie DeMonia, Martin T. Ferris, Samir N.P. Kelada, Jason K. Whitmire
,
GE Healthcare
,Cat#
17-1440-02
Ghost Dye Red 780
Viability Dye,
TONBO
,Cat#
13-0865-T100
GoTaq Green,
Promega
,Cat#
M7122
HEPES,
Lonza
,Cat#
17-737E
Ionomycin,
Sigma Aldrich
,Cat# I9657
L-glutamine
,Lonza,Cat#17-605L
Magnesium chloride
(3)
Establishment of paternal methylation imprint at the H19/Igf2 imprinting control region
Science Advances
September 6, 2023
Ji Liao, Sangmin Song, Samuel Gusscott, Zhen Fu, Ivan VanderKolk, Brianna M. Busscher, Kin H. Lau, Julie Brind’Amour, Piroska E. Szabo´
performed with 1 μl of digested tail complex in 10 μl of total volume including 2×
GoTaq Green Master Mix
(
M7122
,
Promega
) using the
PCR
cycles: 95°C for 2 min, (95°C, 30 s, 65°C, 45 s, 72°C, 45 s) for 40 cycles and
(4)
DMC1 stabilizes crossovers at high and low temperatures during wheat meiosis
Front Plant Sci
August 8, 2023
Tracie N. Draeger, María-Dolores Rey, Sadiye Hayta, Mark Smedley, Azahara C. Martin, Graham Moore
5′-TCAATCCCT CCTTCAAATTACGC-3′ were used.
PCR amplification
was performed using GoTaq® Master Mix (
Promega
,
M7122
), with the following conditions: 3 min 94 °C, 40 cycles of 30 s at 94 °C, 15 s at 58 °C, 1 min at 72
(5)
Liver-specific in vivo base editing of Angptl3 via AAV delivery efficiently lowers blood lipid levels in mice
Cell & bioscience
June 15, 2023
Zuo Y, Zhang C, Zhou Y, Li H, Xiao W, Herzog RW, Xu J, Zhang J, Chen YE, Han R.
or 100 ng genomic DNA was used as template in regular
PCR reactions
using GoTaq Master Mix (
Promega
,
M7122
). The
PCR
products were purified by
Wizard SV Gel and PCR Clean-Up System
(
Promega
,
A9285
) according
(6)
CRISPR-Cas9 correction of a nonsense mutation in LCA5 rescues lebercilin expression and localization in human retinal organoids
Molecular Therapy - Methods & Clinical Development
June 8, 2023
Tess A.V. Afanasyeva, Dimitra Athanasiou, Pedro R.L. Perdigao, Kae R. Whiting, Lonneke Duijkers, Galuh D.N. Astuti, Jean Bennett, Alejandro Garanto, Jacqueline van der Spuy, Ronald Roepman,
et al.
5′-TCAACCATGCAACACAGTGAAGCT-3′ (reverse).
PCR reactions
were carried out with 100 ng genomic DNA in
GoTaq Green Master Mix
(
M7122
,
Promega
) according to the manufacturer’s instructions.
PCR
thermocycling scheme was as follows: 5 min
(7)
Combined lineage tracing and scRNA-seq reveals unexpected first heart field predominance of human iPSC differentiation
eLife
June 7, 2023
Francisco Xavier Galdos, Carissa Lee, Soah Lee, Sharon Paige, William Goodyer, Sidra Xu, Tahmina Samad, Gabriela V Escobar, Adrija Darsha, Aimee Beck,
et al.
construct to be integrated. PCRs were conducted using the GoTaq Master Mix (
Promega Corporation
Cat.
M7122
), and products were run on a 1% agarose gel in 1 X TAE Buffer.
PCR
primer sequences are found in Figure
(8)
An unusual tandem kinase fusion protein confers leaf rust resistance in wheat
Nature Genetics
June 1, 2023
Yajun Wang, Michael Abrouk, Spyridon Gourdoupis, Dal-Hoe Koo, Miroslava Karafiátová, István Molnár, Kateřina Holušová, Jaroslav Doležel, Naveenkumar Athiyannan, Emile Cavalet-Giorsa,
et al.
8).
PCR
amplifications were done in 20 μl reactions containing 1×
GoTaq Green Master Mix
(
Promega
,
M7122
), ∼100 ng genomic DNA and 100 nM primers. Primer pairs K1-F/K1-R, K2-F2/K2-R1 and
vWA
-F2/
vWA
-R1 were
(9)
Establishment and inheritance of minichromosomes from Arabidopsis haploid induction
Chromosoma
June 1, 2023
Ek Han Tan, Benny Ordoñez, Tejas Thondehaalmath, Danelle K. Seymour, Julin N. Maloof, Ravi Maruthachalam, Luca Comai
μL reaction volumes and recommended primer pair concentrations using GoTaq Green Mastermix (
Promega
M7122
) and 55 °C annealing temperatures for 40 cycles. After
PCR
, a 5 μL digestion mix containing 0.1 μL restriction
(10)
The Occurrence of Folate Biosynthesis Genes in Lactic Acid Bacteria from Different Sources
Food Technology and Biotechnology
June 1, 2023
Fenny Amilia Mahara, Lilis Nuraida, Hanifah Nuryani Lioe, Siti Nurjanah
detection
PCR
amplifications were performed in 20 µL volumes with 10 µL Promega Go Taq Green Master Mix 1× (
M7122
;
Promega Corporation
), containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers, 1 µL of 0.5
(11)
Multi-omics analysis of a Bacteroides fragilis isolate from an ulcerative colitis patient defines genetic determinants of fitness in bile
bioRxiv
May 16, 2023
Aretha Fiebig, Matt Schnizlein, Selymar Pena-Rivera, Florian Trigodet, Abhishek Anil Dubey, Miette Hennessy, Anindita Basu, Sebastian Pott, Sushila Dalal, David Rubin,
et al.
clones
were mapped using a two-step nested arbitrary
PCR
approach using 2X GoTaq master mix (
Promega
M7122
). The first reaction (1X GoTaq master mix, 0.3 uM U1 fw, 0.3 uM M13-N7 and 0.5 ul saved culture in a
(12)
Molecular Evaluation of the Impact of Polymorphic Variants in Apoptotic (Bcl-2/Bax) and Proinflammatory Cytokine (TNF-α/IL-8) Genes on the Susceptibility and Progression of Myeloproliferative Neoplasms: A Case-Control Biomarker Study
Current Issues in Molecular Biology
May 2, 2023
Mamdoh S. Moawadh, Rashid Mir, Faris J. Tayeb, Orooba Asim, Mohammad Fahad Ullah
25 pmol each primer, and 10 μL of
GoTaq® Green Master Mix
from
Promega
, Fitchburg, WI, USA (cat no
M7122
). The reaction mixture also received 2 μL of DNA from either the patient or control sample, and nuclease-free
(13)
Genetic profile of thymic epithelial tumors in the Japanese population: an exploratory study examining potential therapeutic targets
Translational Lung Cancer Research
April 28, 2023
Midori Shimada, Hirokazu Taniguchi, Hiroyuki Yamaguchi, Hiroshi Gyotoku, Daisuke Sasaki, Norihito Kaku, Chikako Senju, Hiroaki Senju, Erika Imamura, Shinnosuke Takemoto,
et al.
using Primer3 Input (ELIXIREstonia, Tartu, Estonia). The primers,
GoTaq® Green Master Mix
(Catalog #
M7122
,
Promega
, Madison, WI, USA), water, and each DNA sample were thoroughly mixed, and subsequent
amplification
(14)
eYGFPuv-Assisted Transgenic Selection in Populus deltoides WV94 and Multiplex Genome Editing in Protoplasts of P. trichocarpa × P. deltoides Clone ‘52-225’
Plants (Basel)
April 14, 2023
Guoliang Yuan, Yang Liu, Tao Yao, Wellington Muchero, Jin-Gui Chen, Gerald A. Tuskan, Xiaohan Yang
Transgenic plants
were
PCR
genotyped using GoTaq® Master Mixes (
Promega
, Madison, WI, USA, Cat. No.
M7122
). We used 1 μL of genomic DNA as a template with the following
PCR
cycling conditions: 95 °C for 2 min,
(15)
Mir125b-2 imprinted in human but not mouse brain regulates hippocampal function and circuit in mice
Communications biology
March 14, 2023
Chou MY, Cao X, Hou KC, Tsai MH, Lee CY, Kuo MF, Wu VC, Huang HY, Akbarian S, Chang SK,
et al
.
(primer sequences are listed in Supplementary Data 5) and
Taq polymerase
(GoTaq® Master Mixes (
Promega
,
M7122
); or AptaTaq™ Fast DNA Polymerase (Roche, 6879110001) for some of the samples), in
C1000 Touch
Thermal
(16)
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
Journal of Visualized Experiments
February 10, 2023
Brittney Voigt, Ryoko Minowa, Ryan S. Gray
oval divot",Generic,n/a Stereomicroscope,Zeiss,
Stemi
508 "
Taq polymerase
master mix, 2X",
Promega
,
M7122
"TBE Buffer Concentrate, 10X",VWR,E442 Thermal Cycler,
Bio-Rad
,
1861096
Tissue paper,Fisher Scientific,06–666 "Tricaine-methanesulfonate
(17)
Multiplex CRISPR-Cas9 Gene-Editing Can Deliver Potato Cultivars with Reduced Browning and Acrylamide
Plants (Basel)
January 13, 2023
Diem Nguyen Phuoc Ly, Sadia Iqbal, John Fosu-Nyarko, Stephen Milroy, Michael G. K. Jones
protocol. The PCRs were conducted with the
GoTaq® Green Master Mix
(
Promega Corporation
, catalogue no.
M7122
) and used 300 ng of cDNA or 100 ng of DNA for each
potato
cultivar in separate 20 µL reactions. The
(18)
The cytokine receptor CRLF3 is a human neuroprotective EV-3 (Epo) receptor
Frontiers in molecular neuroscience
January 1, 2023
Knorr DY, Rodriguez Polo I, Pies HS, Schwedhelm-Domeyer N, Pauls S, Behr R, Heinrich R.
using specific primers as stated below (Table 1).
PCR
was run using
GoTaq Green Master Mix
(
Promega
; #
M7122
) and
PCR
program was set as shown in Table 2.
PCR
products were loaded on a 1% agarose gel and run for
(19)
A standalone incompatible insect technique enables mosquito suppression in the urban subtropics
Communications Biology
December 27, 2022
Qin Zeng, Lingzhi She, Hao Yuan, Yuying Luo, Renke Wang, Wei Mao, Weifeng Wang, Yueting She, Chaojun Wang, Mengyi Shi,
et al.
and ND5 gene
amplification
on a
Bio-Rad
T100 thermal cycler
. Using
GoTaq Green Master Mix
(
Promega
M7122
), mitochondrial COI gene was amplified with specific primers LCO1490 and HCO219836 as follows: initial
(20)
Metabolite Profiling of Gardenia jasminoides Ellis In Vitro Cultures with Different Levels of Differentiation
Molecules
December 14, 2022
Gergana Krasteva, Strahil Berkov, Atanas Pavlov, Vasil Georgiev
were selected for this study (Table S4). For
PCR amplification
,
GoTaq Green Master Mix
(
Promega
, cat.#
M7122
) was used according to the kit’s manual. The reaction mixture (25 µL) consisted of 12.5 µL
GoTaq Green Master Mix
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Reviews
Write a Review
(1)
7/17/2020
Efficient Amplification of DNA Using GoTaq® Green Master Mix
Xiaoguang Li
I recommend this because it saves a lot of time, and works well. I didn't recycle the gel product.
(1)
7/23/2019
Promega's PCR Master Mix for Colony PCR Works Well
Atul Singh
Best kit I have used so far for colony PCR.
(1)
1/28/2019
Very Easy and Efficient Taq Mastermix
Virginia Folgado
I needed a cheap and efficient PCR mix since I have to do a lot of PCR from bacteria colonies to check presence/absence of my target insert in the plasmid. I found this kit very easy to use; I didn't ...
Read Review
(1)
8/15/2017
Great PCR Master Mix
Chris C
We use this PCR master mix for genotyping PCR reaction for transgenic mice. We selected this product because of its ease of use, just add primers, water and template. It works well for our PCR ...
Read Review
(1)
12/29/2016
Super Easy PCR Premix
Zeji Du
GoTaq Green Master Mix is a premixed ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by ...
Read Review
(1)
10/14/2015
Premixed and Ready-To-Use Solution for DNA Synthesis
Sunita Meena
GoTaq® Green Master Mix was mixed with primers which are specific for the gene of interest present in cDNA and kept in PCR machine for amplification. The PCR product was directly visualized in ...
Read Review
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GoTaq Green Master Mix from Promega
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