GoTaq Hot Start Colorless Master Mix from Promega

Product Specs
ItemGoTaq Hot Start Colorless Master Mix
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM5132
Quantity100 reactions
Concentration2X

Specifications/Features

Information:

Biological Source for GoTaq® Hot Start Polymerase: The enzyme is derived from bacteria. The antibody is derived
from murine cell culture.
Storage Conditions: See the Product Information Label for storage recommendations. Minimize the number of freezethaw
cycles by storing in working aliquots. Product may be stored at 4°C for up to 12 weeks. Mix well prior to use.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) The diversity of cavity nesting bees (Hymenoptera: Megachilidae) recolonizing a post-wildfire landscape and their pollen provisions, with reference to aerial seed mix applicationsAnnals of the Entomological Society of AmericaJanuary 28, 2026Thomas S Davisµl PCR reaction was mixed according to the Promega PCR Master Mix specifications (Promega catalog # M5133, Madison, Wisconsin) which included 0.4 µM of each primer and 1 µl of gDNA. DNA was PCR amplified using

(2) Forage preference in two geographically co-occurring fungus gardening ants: A dietary DNA approachPLoS OneDecember 4, 2025Matthew Richards-Perhatch V, Elizabeth Boshers, Matthew Greenwold, Jon N. SealPCR reaction was mixed according to the Promega PCR Master Mix specifications (Promega, Catalog No. M5133, Madison, WI) which included 0.4 µM of each primer and 1 µl of gDNA. DNA was PCR amplified using the

(3) Surveying tropical faunal diversity via airborne DNA analysesSci RepOctober 21, 2025Joseph M. Craine, Nicholas Schulte, Jessica Devitt, Devin Leopold, Kristin Saltonstall, Noah Fiereramplified. Each 25 µL PCR reaction was mixed according to the Promega PCR Master Mix specifications (Promega # M5133, Madison, WI) which included 12.5 µL Master Mix, 0.5 µL of each 10 µM primer, 2.0 µL of gDNA, and 10.5

(4) Stabilized and unstabilized sampling methods result in differential fecal 16S rRNA microbial sequencing resultsPLoS OneAugust 13, 2025Christopher E. Stamper, Andrew J. Hoisington, Joseph C. Ellis, Christopher A. Lowry, Lisa A. Brennergenes in isolated DNA were polymerase chain reaction (PCR)-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, WI, USA) and 515 F (5′-GTGCCAGCMGCCGCGGTAA-3′), 806 R (5′-GGACTACHVGGGTWTCTAAT-3′)

(5) Dietary composition and overlap between cattle and endangered mountain gazelle (Gazella gazella)Sci RepJune 6, 2025Amir Arnon, Torsten Wronski, Maya Lalzar, S. Yan Landau, Ido Izhaki, Dan MalkinsonµL PCR reaction was mixed according to the Promega PCR Master Mix specifications (Promega catalog # M5133, Madison, WI), which included 0.4 µM of each primer and 1 µl of gDNA. DNA was PCR amplified using the

(6) High diversity of fungal ecological groups from ice-free pristine and disturbed areas in the Fildes Peninsula, King George Island, AntarcticaPLoS OneJanuary 22, 2025Sigisfredo Garnica, Genaro Soto-Rauch, Ellen M. Leffler, Christian Núñez, Jonhatan Gómez-Espinoza, Enzo Romero, Ramón Ahumada-Rudolph, Jaime R. Cabrera-PardoPCR reaction was prepared according to the Promega PCR Master Mix specifications (Promega catalog # M5133, Madison, WI), consisting of 12.5 μL Master Mix, 0.5 μL of each primer, 1.0 μL of genomic DNA, and 10.5

(7) Quantifying variation across 16S rRNA gene sequencing runs in human microbiome studiesApplied Microbiology and BiotechnologyDecember 1, 2024Andrew J. Hoisington, Christopher E. Stamper, Joseph C. Ellis, Christopher A. Lowry, Lisa A. Brennergenes in isolated DNA were polymerase chain reaction (PCR)-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, WI, USA) and 515 F (5′-GTGCCAGCMGCCGCGGTAA-3′), 806 R (5′-GGACTACHVGGGTWTCTAAT-3′)

(8) Recent alcohol intake impacts microbiota in adult burn patientsAlcoholAugust 1, 2024Andrew J. Hoisington, Kevin Choy, Shanawaj Khair, Kiran U. Dyamenahalli, Kevin M. Najarro, Arek J. Wiktor, Daniel N. Frank, Ellen L. Burnham, Rachel H. McMahan, Elizabeth J. Kovacsgenes in isolated DNA were polymerase chain reaction (PCR)-amplified using GoTaq Master Mix (Cat.No. M5133, Promega, Madison, Wisconsin) with 338F F (5′-ACTCCTACGGGAGGCAGCAG-3′) and 806 R (5′- GGACTACHVGGGTWTCTAAT-3′)

(9) Disturbances of Ruminal Microbiota and Liver Inflammation, Mediated by LPS and Histamine, in Dairy Cows Fed a High-Concentrate DietAnimals (Basel)May 17, 2024Nana Ma, Junfei Guo, Zhenfu Li, Lei Xu, Kai Zhang, Tianle Xu, Guangjun Chang, Juan J. Loor, Xiangzhen Shen(5′-GACTACHVGGGTATCTAATCC-3′). PCR amplification was run using the GoTaq® Hot Start Colorless Master Mix (M5133, Promega, Madison, WI, USA) apparatus and the procedure was as follows: 95 °C 2 min (first pre-degeneration),

(10) Association of homelessness and diet on the gut microbiome: a United States-Veteran Microbiome Project (US-VMP) studymSystemsJanuary 23, 2024Andrew J. Hoisington, Kelly A. Stearns-Yoder, Christopher E. Stamper, Ryan Holliday, Diana P. Brostow, Molly E. Penzenik, Jeri E. Forster, Teodor T. Postolache, Christopher A. Lowry, Lisa A. Brennerin isolated DNA were polymerase chain reaction (PCR) amplified using GoTaq Master Mix (catalog no. M5133, Promega, Madison, WI) and 515 F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806 R (5′-GGACTACHVGGGTWTCTAAT-3′)

(11) Food desert residence has limited impact on veteran fecal microbiome composition: a U.S. Veteran Microbiome Project studymSystemsDecember 21, 2023Diana P. Brostow, Meghan Donovan, Molly Penzenik, Christopher E. Stamper, Talia Spark, Christopher A. Lowry, Suzanne L. Ishaq, Andrew J. Hoisington, Lisa A. Brenner[29]). Marker genes in isolated DNA were PCR-amplified in duplicate using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, WI, USA) as described in Caporaso et al. (32) using primers 515F–808R. Successful

(12) Assessing springtime vertebrate prey of sympatric mesopredators in the southeastern United States using metabarcoding analysisPLOS ONEOctober 25, 2023Jordan L. Youngmann, Stacey L. Lance, John C. Kilgo, Charles Ruth, Jay Cantrell, Gino J. D’AngeloIllumina sequencing [69]. The 25 μL PCR reactions followed Promega PCR Master Mix specifications (Promega catalog # M5133, Madison, WI), including 12.5ul of Master Mix, 0.5 μl of each primer, 1.0 μl of gDNA, and 10.5 μl of

(13) Water temperature and disease alters bacterial diversity and cultivability from American lobster (Homarus americanus) shellsiScienceMay 19, 2023Suzanne L. Ishaq, Sarah M. Turner, Grace Lee, M. Scarlett Tudor, Jean D. MacRae, Heather Hamlin, Deborah Bouchard(5′-GTGYCAGCMGCCGCGGTAA) and 806R (5′-GGACTACNVGGGTWTCTAAT),79 and Promega PCR Master Mix specifications (Promega catalog #M5133, Madison, WI) which included 12.5ul Master Mix, 0.5 μL of each primer, 1.0 μL of gDNA, and 10.5 μL DNase/RNase-free

(14) Impacts of breast cancer and chemotherapy on gut microbiome, cognitive functioning, and mood relative to healthy controlsSci RepNovember 15, 2022Emily Bilenduke, John D. Sterrett, Krista W. Ranby, Virginia F. Borges, Jim Grigsby, Alaina L. Carr, Kristin Kilbourn, Christopher A. Lowrygenes in isolated DNA were polymerase chain reaction (PCR) amplified using GoTaq Master Mix (Cat No. M5133, Promega, Madison, WI, USA); 515 F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806 R (5′-GGACTACHVGGGTWTCTAAT-3′)

(15) Single-cell transcriptome analysis reveals three sequential phases of gene expression during zebrafish sensory hair cell regenerationDevelopmental CellMarch 21, 2022Sungmin Baek, Nhung T. T. Tran, Daniel C. Diaz, Ya-Yin Tsai, Joaquin Navajas Acedo, Mark E. Lush, Tatjana Piotrowski,Thermo Fisher Scientific,D3571 Draq5,Biostatus,DR50200 MeOH,Sigma-Aldrich,34860-1L-R; CAS: 67-56-1 Protector RNase inhibitor,Roche,03335399001 GoTaq Hot Start Master Mix,Promega,M5132 T3 RNA polymerase,Promega,P2083 Transcription optimized 5x buffer,Promega,P1181 DTT (100mM),Promega,P1171 DIG

(16) Inherited L1 Retrotransposon Insertions Associated With Risk for Schizophrenia and Bipolar DisorderSchizophrenia Bulletin OpenDecember 13, 2021Benjamin C Reiner et al.confirmation). 25 µl confirmatory PCR reactions contained 1× Go-Taq hotstart colorless master mix (#M5133, Promega), 1 ng of gDNA, 0.2 µM L1HsACA primer (supplementary table 2), and 0.2 µM L1 insertion specific

(17) Characterization of the gut microbiota among Veterans with unique military-related exposures and high prevalence of chronic health conditions: A United States-Veteran Microbiome Project (US-VMP) studyBrain, Behavior, & Immunity - HealthDecember 1, 2021Maggie A. Stanislawski, Christopher E. Stamper, Kelly A. Stearns-Yoder, Andrew J. Hoisington, Diana P. Brostow, Jeri E. Forster, Teodor T. Postolache, Christopher A. Lowry, Lisa A. BrennerValencia, CA, USA). Marker genes in isolated DNA were PCR-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, WI, USA) as described in Caporaso et al. (2012) PCR products were cleaned and normalized

(18) Geographic source estimation using airborne plant environmental DNA in dustScientific ReportsDecember 1, 2021Chelsea Lennartz, Joel Kurucar, Stephen Coppola, Janice Crager, Johanna Bobrow, Laura Bortolin, James Comollisequences. Each 25 μl amplification reaction consisted of 12.5 μl GoTaq PCR mastermix (Promega cat # M5133), 0.5 μl each forward and reverse primer (0.2 μM final concentration), 1 μl extracted eDNA or extraction

(19) Seasonal patterns of bison diet across climate gradients in North AmericaScientific ReportsDecember 1, 2021Joseph M. CraineµL PCR reaction was mixed according to the Promega PCR Master Mix specifications (Promega catalog # M5133, Madison, WI) which included 0.4 µM of each primer and 1 µl of gDNA. Both forward and reverse primers

(20) Processing human urine and ureteral stents for 16S rRNA amplicon sequencingSTAR ProtocolsJune 18, 2021Kait F. Al, Jeremy P. Burton7003PK Nuclease free water,Ambion,Catalog No. AM9932 GoTaq Hot start Colorless Master Mix,Promega,Catalog No. M5133 "Ethanol, 99%",Fisher Chemical,Catalog No. BPA9954 ,, Critical commercial assays,Critical commercial

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12/26/2019Colony PCR Screening Using GoTaq Hot Start PCR KitDr. Thippeswamy SannasiddappaGoTaq Hot Start PCR kit is ideal kit to be in the molecular biology lab especially colony PCR workstream.