Phusion GC Buffer Pack from New England Biolabs

Product Specs
ItemPhusion GC Buffer Pack
CompanyNew England Biolabs
Price $27.00 Inquire
Catalog NumberB0519S
Quantity6 ml
ApplicationsDNA Analysis
TypeBuffer
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A One Health approach to bovine coronavirus vaccine development, using LSDV as a live virus vectorHuman Vaccines & ImmunotherapeuticsDecember 31, 2025Nicole Chineka, Leah Whittle, Nicola Douglass, Anna-Lise Williamson, Ros Chapman(Zymo Research, D7020). Phusion High-Fidelity enzyme was used with the GC Buffer (New England BioLabs, B0519S) and 3% DMSO (New England BioLabs, B0519S). PCR 1 was run according to the following parameters: a single

(2) Bisubstrate Analog Inhibitors of DXP Synthase Show Species SpecificityBiochemistryJanuary 21, 2025Stephanie Henriquez, Charles R. Nosal, Joseph R. Knoff, Lauren B. Coco, Caren L. Freel Freel MeyersDNA. PCR mixtures (total volume 50 μL) were incubated on ice and contained 1X Phusion GC buffer (NEB# B0519S), 2 ng/μL genomic DNA, 0.2 mM dNTPs, 0.5 μM forward and reverse primers (Padxs_C-H6_1 and Padxs_C-H6_2,

(3) Engineering an fgfr4 knockout zebrafish to study its role in development and diseasePLOS ONENovember 22, 2024Emma N. Harrison, Amanda N. Jay, Matthew R. Kent, Talia P. Sukienik, Collette A. LaVigne, Genevieve C. Kendallfor 10 minutes, and held at 4°C. The PCR reaction mix was created with NEB Phusion GC Buffer (NEB, B0519S), Phusion DNA Polymerase (NEB, M0530S), 10mM dNTPs (Fisher Scientific, 10-297-018), 10 μM forward primer

(4) Targeting PD-L2-RGMb overcomes microbiome-related immunotherapy resistanceNatureMay 1, 2023Park JS, Gazzaniga FS, Wu M, Luthens AK, Gillis J, Zheng W, LaFleur MW, Johnson SB, Morad G, Park EM, et al.bacteria sequence, 2 μl of bacterial DNA was mixed with 10 μl 5x GC Buffer (New England BioLabs Cat#B0519S) +1μ1 of 1 μM dNTPs + 2.5 μl each of 10 μM forward (27F AGA GTT TGA TCM TGG CTC AG) and reverse primers

(5) Structure-function analysis of TOPBP1's role in ATR signaling using the DSB-mediated ATR activation in Xenopus egg extracts (DMAX) systemScientific reportsJanuary 11, 2021Montales K, Kim A, Ruis K, Michael WM.(5′-AGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTC-3′) primers along with 0.4 mM dNTPs (volume 4 µl), 20 µl of Phusion GC Buffer (NEB #B0519S),1 µL Phusion High-Fidelity DNA Polymerase (NEB #M0530S), and 63 µl of water. The annealing temperature

(6) A transient CRISPR/Cas9 expression system for genome editing in Trypanosoma bruceiBMC Research NotesDecember 1, 2020Sebastian Shaw, Sebastian Knüsel, Sarah Hoenner, Isabel Roditireagents from New England Biolabs: Phusion High-Fidelity DNA polymerase (M0530S), 5 x Phusion HF buffer (B0519S) and dNTP mix (N0447S). Cycling conditions are identical to those previously published [4, 13]. – 1st

(7) Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiaeMethods in Molecular BiologyMarch 28, 2020Erica J. Polleys, Catherine H. Freudenreichthat has been used in amplification of the CAG repeat tract is Phusion GC Buffer (New England Biolabs, B0519S) 2.In order to obtain robust tract lengths PCR, primers should be designed such that there is a region

(8) Comprehensive Protocols for CRISPR/Cas9‐based Gene Editing in Human Pluripotent Stem CellsCurrent Protocols in Stem Cell BiologyAugust 18, 2016David P. Santos, Evangelos Kiskinis, Kevin Eggan, Florian T. Merkle,Thermo Fisher Scientific,B72 FastDigest Esp3I (BsmBI),Thermo Fisher Scientific,FD0454 GC Buffer (5X),NEB,B0519S "Gel Loading Dye, Purple (6X)",NEB,B7024S "Geltrex, hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix

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