PacI from New England Biolabs

Product Specs
ItemPacI
CompanyNew England Biolabs
Price $76.00 Inquire
Catalog NumberR0547S
Concentration4 u/µL
Quantity250 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

TTAAT/TAA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(61) An inactivated novel chimeric FAdV-4 containing fiber of FAdV-8b provides full protection against hepatitis-hydropericardium syndrome and inclusion body hepatitisVeterinary ResearchSeptember 30, 2022Baiyu Wang, Mingzhen Song, Congcong Song, Shiyi Zhao, Panpan Yang, Qilong Qiao, Yanfang Cong, Yanling Wang, Zeng Wang, Jun ZhaoThe correct clone named p15A-cm-HNJZ-fiber-1-amp-ccdB was linearized with PacI (NEB, USA, Cat. no. R0547). Next, fiber gene of FAdV-8b was amplified from the genome of FAdV-8b strain SW2021 using primers Fiber/8b-F

(62) Multiplexed promoter and gene editing in wheat using a virusâ€based guide RNA delivery systemPlant Biotechnology JournalSeptember 7, 2022Wei Wang, Zitong Yu, Fei He, Guihua Bai, Harold N. Trick, Alina Akhunova, Eduard Akhunovremoving the PDS fragment by PacI and NotI digestion (New England BioLabs, Ipswich, MA, catalogue numbers R0547 and R0189). The sequences targeted in our study are listed in Table S2. All the primers, DNA oligos

(63) A moonlighting function of a chitin polysaccharide monooxygenase, CWR-1, in Neurospora crassa allorecognitioneLifeAugust 30, 2022Tyler C Detomasi, Adriana M Rico-RamÃrez, Richard I Sayler, A Pedro GonÃ§alves, Michael A Marletta, N Louise Glass5′ and 3′ ends, respectively. Amplified and gel-purified PCR ORFs were XbaI (R0145S, NEB) and PacI (R0547S, NEB) digested and inserted into XbaI/PacI digested pMF272::his-3-Ptef-1-V5-Tccg-1. The fragments were

(64) Prime editor-mediated correction of a pathogenic mutation in purebred dogsScientific ReportsJuly 28, 2022Dong Ern Kim, Ji Hye Lee, Kuk Bin Ji, Eun Ji Lee, Chuang Li, Hyun Ju Oh, Kang Sun Park, Seung Hoon Lee, Okjae Koo, Min Kyu Kimtargeting the HD locus was newly synthesized and then added to the vector using PacI (NEB Inc., MA, USA: #R0547S) and EcoRI. Finally, the vector was confirmed through sequencing. The lentiviral particles of PE vector

(65) Targeted removal of mitochondrial DNA from mouse and human extrachromosomal circular DNA with CRISPR-Cas9Computational and Structural Biotechnology JournalJune 15, 2022Weijia Feng, Gerard Arrey, Egija Zole, Wei lv, Xue Liang, Peng Han, Marghoob Mohiyuddin, Henriette Pilegaard, Birgitte Regenbergenzymatically, digestions were performed with the restriction enzyme PacI (New England Biolabs, Cat. No.: R0547) and the restriction enzyme MssI (New England Biolabs, Cat. No.: R0560S) following the manufacturer's

(66) Humanized mice reveal a macrophage-enriched gene signature defining human lung tissue protection during SARS-CoV-2 infectionCell reportsApril 19, 2022Kenney DJ, O'Connell AK, Turcinovic J, Montanaro P, Hekman RM, Tamura T, Berneshawi AR, Cafiero TR, Al Abdullatif S, Blum B, et al.Certified, 10% (v/v)",Fisher,LC146705 Tween-20 10% solution,Teknova,T0710 PacI,New England BioLabs (NEB),R0547L MluI-HF,New England BioLabs (NEB),R3198L PEG8000,Sigma,81268 Spermine,Sigma,S3256 Benzonase,Sigma,E1014 Iodixanol,Sigma,D1556 Pluronic F-68

(67) AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse BrainJournal of Visualized ExperimentsApril 18, 2022Tracy L. Warren, Jason T. Lambert, Alex S. Nordrestriction digest with PacI, AscI, and XmaI" Restriction enzyme: AscI,NEB,R0558L, Restriction enzyme: PacI,NEB,R0547L, Restriction enzyme: XmaI,NEB,R0180L, SOC outgrowth medium,NEB,B0920S,Recovery medium after transformation Sucrose

(68) miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded multiplex RNAi at a single-cell levelEMBO reportsApril 5, 2022Wang D, Xiu J, Zhao J, Luo J.enzyme,NEB,R0652S ClaI restriction enzyme,NEB,R0197V KpnI‐HF restriction enzyme,NEB,R3142V PacI restriction enzyme,NEB,R0547S PmeI restriction enzyme,NEB,R0560V T4 DNA ligase,NEB,M0202S 100 bp ladder,NEB,N3231V MojoSort™ Mouse CD8 T Cell Isolation Kit

(69) The C terminus of the mycobacterium ESX-1 secretion system substrate ESAT-6 is required for phagosomal membrane damage and virulenceProceedings of the National Academy of Sciences of the United States of AmericaMarch 15, 2022Osman MM, Shanahan JK, Chu F, Takaki KK, Pinckert ML, Pagán AJ, Brosch R, Conrad WH, Ramakrishnan L.

(70) Transcriptomic taxonomy and neurogenic trajectories of adult human, macaque, and pig hippocampal and entorhinal cellsNeuronFebruary 2, 2022Franjic D, Skarica M, Ma S, Arellano JI, Tebbenkamp ATN, Choi J, Xu C, Li Q, Morozov YM, Andrijevic D, et al.,Sino Biological,Cat#13030-H09E Digoxigenin-UTP,Roche,Cat#11209256910 BsrGI,New England BioLabs,Cat#R0575L PacI,New England BioLabs,Cat#R0547L Klenow,New England BioLabs,Cat#M0210M "Trypsin Gold, Mass Spectrometry Grade",Promega,Cat#V5280 PolyJet,SignaGen,Cat#SL100688 Protamine

(71) Parallel functional testing identifies enhancers active in early postnatal mouse braineLifeOctober 4, 2021Lambert JT, Su-Feher L, Cichewicz K, Warren TL, Zdilar I, Wang Y, Lim KJ, Haigh JL, Morse SJ, Canales CP, et al.pooling. We next linearized the vector pscAAV-HspMinP-EGFP (Figure 1—figure supplement 1) using PacI (NEB #R0547L) and AscI (NEB #R0558L). AAV DNA vectors are prone to recombination at the ITR sites, so we also performed

(72) Tissue-specific DamID protocol using nanopore sequencingJ Biol MethodsAugust 27, 2021Georgina Gómez-Saldivar, Dominique A. Glauser, Peter Meister3’UTR]✓ C. elegans gDNA or vector bearing the gene or promoter of interest.✓ PacI restriction enzyme (R0547S, NEB)✓ KpnI restriction enzyme (R3142S, NEB)✓ NruI restriction enzyme (R3192S, NEB)✓ NotI restriction

(73) Proof of Gene Doping in a Mouse Model with a Human Erythropoietin Gene Transferred Using an Adenoviral VectorGenesAugust 16, 2021Sugasawa T, Nakano T, Fujita SI, Matsumoto Y, Ishihara G, Aoki K, Yanazawa K, Ono S, Tamai S, Manevich L, et al.Finally, the obtained plasmid (pAd/CMV/V5-DEST-hEPO) was digested using a Pac1 restriction enzyme (Cat# R0547; New England Biolabs, Ipswich, MA, USA) and transfected into HEK 293A cells (Cat# R70507; Thermo Fisher Scientific

(74) The Polycomb group protein MEDEA controls cell proliferation and embryonic patterning in ArabidopsisDevelopmental CellJuly 12, 2021Sara Simonini, Marian Bemer, Stefano Bencivenga, Valeria Gagliardini, Nuno D. Pires, Bénédicte Desvoyes, Eric van der Graaff, Crisanto Gutierrez, Ueli Grossniklausenzyme-containing mixes ,, BsaI-HF v2,NEB,Catalog # R3733S EcoRV,NEB,Catalog # R0195L PacI,NEB,Catalog # R0547S XhoI,NEB,Catalog #R0146M SmaI,NEB,Catalog #R0141L SalI,NEB,Catalog #R0138M AscI,NEB,Catalog # R0558S SphI,NEB,Catalog

(75) MSCV-based retroviral plasmids expressing 3xFLAG-Sp-dCas9 for enChIP analysisBiology Methods and ProtocolsJuly 9, 2021Miyuki Yuno, Shoko Nagata, Toshitsugu Fujita, Hodaka Fujiiisolated from 3xFLAG-dCas9/pMXs-puro (Addgene, Watertown, MA, USA, #51240) [13] by digestion with Pac I (R0547, New England Biolabs, Ipswich, MA, USA) and Not I (1166A, Takara Bio). To construct gRNA-hIRF-1 #12/pSIR-human

(76) In pancreatic β-cells myosin 1b regulates glucose-stimulated insulin secretion by modulating an early step in insulin granule trafficking from the GolgiMolecular Biology of the CellJune 1, 2021Hiroshi Tokuo, Shigeru Komaba, Lynne M. Coluccio(S9106S) were obtained from New England Biolabs (Ipswich, MA). Restriction enzymes PmeI (R0560S), PacI (R0547S), BglII (R0144S), and XhoI (R0146S) were also obtained from New England Biolabs. Lipofectamine and Lipofectamine

(77) A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoirCell reports. MedicineApril 20, 2021Levy CN, Hughes SM, Roychoudhury P, Reeves DB, Amstuz C, Zhu H, Huang ML, Wei Y, Bull ME, Cassidy NAJ, et al.,New England BioLabs,Cat#R3142S NcoI-HF,New England BioLabs,Cat#R3193S NsiI-HF,New England BioLabs,Cat#R3127S PacI,New England BioLabs,Cat#R0547S SalI-HF,New England BioLabs,Cat#R3138S SbfI-HF,New England BioLabs,Cat#R3642S XmaI,New England BioLabs,Cat#R0180S BssSαI,New

(78) A selective transmembrane recognition mechanism by a membrane-anchored ubiquitin ligase adaptorThe Journal of cell biologyJanuary 4, 2021Arines FM, Hamlin AJ, Yang X, Liu YJ, Li M.resulting fragment and the Ypq1-GFP-Ura3 plasmid digested with NheI (R3131; New England Biolabs) and PacI (R0547; New England Biolabs) were transformed together into yeast and plated onto YNB plates lacking uracil.

(79) Protocol for Genome Editing to Produce Multiple Mutants in WheatSTAR ProtocolsOctober 28, 2020Fumitaka Abe, Yuji Ishida, Hiroshi Hisano, Masaki Endo, Toshihiko Komari, Seiichi Toki, Kazuhiro Satoenzyme, AscI",New England Biolabs,Cat# R0558L "Restriction enzyme, PacI",New England Biolabs,Cat# R0547L "High-Fidelity restriction enzyme, PstI-HF",New England Biolabs,Cat# R3140M CutSmart Buffer,New England Biolabs

(80) The role of structural pleiotropy and regulatory evolution in the retention of heteromers of paralogseLifeAugust 27, 2019Axelle Marchant et al.reagent,pDONR201 (plasmid),Invitrogen,Cat. #11798–014, Recombinant DNA reagent,PacI,New England BioLabs Inc,Cat. #R0547S, Recombinant DNA reagent,SacI,New England BioLabs Inc,Cat. #R0156S, Recombinant DNA reagent,SpeI,New England BioLabs

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PacI

PacI from New England Biolabs

Sequence

New England Biolabs

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PacI from New England Biolabs

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