PacI from New England Biolabs

Product Specs
ItemPacI
CompanyNew England Biolabs
Price $76.00 Inquire
Catalog NumberR0547S
Concentration4 u/µL
Quantity250 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

TTAAT/TAA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) Redox regulation of G protein oligomerization and signaling by the glutaredoxin WG1 controls grain size in riceThe EMBO JournalMay 19, 2025Lijie Liu, Jianqin Hao, Ke Huang, Penggen Duan, Baolan Zhang, Zhihai Chi, Xiaohong Yao, Yunhai Lireagents","Chemicals, Enzymes and other reagents" BamH I,New England Biolabs,R3136S Pac I,New England Biolabs,R0547L Asc I,New England Biolabs,R0558S T4 DNA ligase,New England Biolabs,M0202S Aar I,Thermo Fisher Scientific,ER1581 Kpn I

(22) Targeted loss of heterozygosity in Candida albicans using CRISPR-Cas9BIORXIVMay 13, 2025Philippe C Després, Nicholas C Gervais, Meea Fogal, Ruby KJ Rogers, Christina A Cuomo, Rebecca S Shapiroin growth phase the following morning. Plasmids were linearized using the PacI enzyme from NEB (cat .R0547L), and left at 37°C for 12h followed by a 20 min heat-inactivation step at 65°C. A transformation master

(23) Identification of regions required for allelic specificity at the cell wall remodeling allorecognition checkpoint in Neurospora crassaGENETICSApril 4, 2025Adriana M Rico-Ramirez, N Louise Glasshomokaryons his-3 conidia from the cwr triple-deletion background were subjected to transformation with PacI (R0547S, NEB), NdeI (R0111S, NEB), or SspI-HF (R3132S, NEB) linearized plasmids. Following established protocols

(24) Efficient transduction of pancreas tissue slices with genetically encoded calcium integratorsbioRxivMarch 29, 2025Charles S. Lazimi, Austin E. Stis, Julia K. Panzer, Helmut Hiller, Maria L. Beery, Amelia K. Linnemann, Cherie L. Stabler, Clayton E. Mathews, Edward A. Phelps(Zymo Research, D4203-B). Plasmids were first linearized with the PacI restriction enzyme (New England Biolabs, R0547L) and precipitated using 100% isopropanol and 70% ethanol. The linearized DNA was transfected into HEK293T

(25) Protocol for evaluating humoral immune responses in mice following SARS-CoV-2 vaccinationSTAR ProtocolsMarch 1, 2025Yao Zhang, Shixiong Li, Jingyou Yupeptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, PacⅠ,NEB,Cat# R0547 Sodium deoxycholate (DOC),Sigma,Cat# D6750 1 M MgCl2,LEAGENE,Cat# R00482 DNaseⅠ,Roche,Cat# 104159 CsCl,Macklin,Cat#

(26) Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell developmentNat CommunFebruary 23, 2025Feifei Yuan, Jihong Yang, Fanglin Ma, Zhe Hu, Vikas Malik, Ruge Zang, Dan Li, Xianle Shi, Xin Huang, Hongwei Zhou, et al.above modified pJet1.2 plasmid were digested with restriction enzymes AgeI (NEB, R0552S) and PacI (NEB, R0547S) and then ligated using T4 DNA ligase (NEB, M0202S) to get pAW63-Tex10 plasmid. The Tex10 guide RNA

(27) Alzheimer-mutant γ-secretase complexes stall amyloid β-peptide productioneLifeFebruary 11, 2025Arafi P, Devkota S, Williams E, Maesako M, Wolfe MS.Laboratories,Cat. No. NLM-467, "Chemical compound, drug",PacI restriction enzyme,New England Biolabs,Cat. No. R0547S, "Chemical compound, drug",SwaI restriction enzyme,New England Biolabs,Cat. No. R0604S, "Chemical

(28) Protocol for generating splice isoform-specific mouse mutants using CRISPR-Cas9 and a minigene splicing reporterSTAR ProtocJanuary 4, 2025Yudong Teng, Kelsey Arbogast, Harald Junge, Zhe Chen,New England Biolabs,R0145L ApaI restriction enzyme,New England Biolabs,R0114L PacI restriction enzyme,New England Biolabs,R0547L EcoRI-HF restriction enzyme,New England Biolabs,R3101L RiboRuler high range RNA ladder,Thermo Fisher Scientific

(29) Alzheimer-mutant γ-secretase complexes stall amyloid β-peptide productionbioRxivNovember 27, 2024Parnian Arafi, Sujan Devkota, Emily Williams, Masato Maesako, Michael S. WolfeCLM-1396 15NH4Cl,Cambridge Isotope Laboratories,Cat. No. NLM-467 PacI restriction enzyme,New England Biolabs,Cat. No. R0547S SwaI restriction enzyme,New England Biolabs,Cat. No. R0604S T4 DNA Polymerase,New England Biolabs,Cat.

(30) Fas Apoptosis Inhibitory Molecule 2 Inhibits Pathological Cardiac Hypertrophy by Regulating the MAPK Signaling PathwayJournal of the American Heart AssociationNovember 19, 2024Huaping Zhong, Minyu Wu, Heping Xie, Xu Chen, Jiayi Li, Zhisheng Duan, Hong Chen, Ziyou Liu, Wei Liao, Yijian Chenrecombinant adenoviral vector (pENTER‐CMV‐Faim2‐ATG‐Flag, pENTER‐U6‐shFaim2) was linearized by PacI (R0547L, NEB) before co‐transfected into HEK293 cells with a polyethyleneimine transfection reagent. The primary

(31) Enhanced Humoral and Cellular Immune Responses Elicited by Adenoviral Delivery of SARS-CoV-2 Receptor-Binding Motif Fused to Human FcVaccines (Basel)November 1, 2024Yea-Jin Lee, Maheswaran Easwaran, Yong-Sam Jung, Yingjuan Qian, Hyun-Jin Shinshuttle vector containing RBM_hFc (Ad/RBM_hFc) were linearized with PacI (New England BioLabs: USA; #R0547S) for 6 h at 37 °C. The linearized vectors were purified using DNA purification kit (QIAGEN: Hilden,

(32) Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid systemWellcome Open ResearchOctober 17, 2024Domenico Modaffari, Aimée Finlayson, Yuyang Miao, Edward W. J. Wallace, Kenneth E. Sawin(Addgene #87845), and pFC333 (Addgene #87844) with restriction enzymes PacI (New England Biolabs, USA; #R0547) and BglII (New England Biolabs, USA; #R0144) and combining them with synthesized gene fragment gDM002

(33) TMEM100 acts as a TAK1 receptor that prevents pathological cardiac hypertrophy progressionCell Communication and SignalingSeptember 11, 2024Bin-Bin Zhang, Yi-Lin Zhao, Yan-Yu Lu, Ji-Hong Shen, Hui-Yong Li, Han-Xue Zhang, Xiao-Yue Yu, Wen-Cai Zhang, Gang Li, Zhan-Ying Han, et al.adenoviral plasmid was obtained according to the above procedure. After linearization with the PacI enzyme (R0547L, NEB), the plasmid was transfected into 293A cells. After 3 rounds of expansion, TMEM100-overexpressing

(34) An improved SNAP-ADAR tool enables efficient RNA base editing to interfere with post-translational protein modificationNature CommunicationsAugust 5, 2024Karthika Devi Kiran Kumar, Shubhangi Singh, Stella Maria Schmelzle, Paul Vogel, Carolin Fruhner, Alfred Hanswillemenke, Adrian Brun, Jacqueline Wettengel, Yvonne Füll, Lukas Funk, et al.Recombinant adenoviral plasmids were verified as containing the gene of interest by Pac-I (NEB, no. R0547L) control digestion. For larger-scale production of recombined plasmids, these were retransformed and

(35) Phosphorylation of GCN2 by mTOR confers adaptation to conditions of hyper-mTOR activation under stressJournal of Biological ChemistryAugust 1, 2024Odai Darawshi, Olaya Yassin, Miri Shmuel, Ronald C. Wek, S. Jalil Mahdizadeh, Leif A. Eriksson, Maria Hatzoglou, Boaz Tiroshare: 5′-AGCTTAATTAAGTCCAAC and GGAGGCAGCGCGGCCGCGGCGA-3′. This plasmid was restricted with PacI (NEB, R0547S) and NotI (NEB, R3189L) and GCN2 was purified and cloned in-frame with 3xFLAG into pLJC2 (Addgene, #87975)

(36) Brewing Coral Terpenes – A Yeast Based Approach to Soft Coral Terpene CyclasesMethods in EnzymologyJune 28, 2024Paul D. Scesa, Eric W. SchmidtGenewiz, etc.) Restriction enzymes: BamHI, PacI, SpeI and XhoI and rCutsmart buffer (NEB # R0136, # R0547, #R3133 and #R0146, respectively) T4 DNA ligase and buffer (NEB # M0202) High fidelity DNA polymerase,

(37) Reconstructing axial progenitor field dynamics in mouse stem cell-derived embryoidsDevelopmental CellJune 1, 2024Adriano Bolondi, Benjamin K. Law, Helene Kretzmer, Seher Ipek Gassaloglu, René Buschow, Christina Riemenschneider, Dian Yang, Maria Walther, Jesse V. Veenvliet, Alexander Meissner, et al.tracing piggyBac library,This study,N/A LentiBC library,This study,N/A PacI,New England Biolabs,Cat# R0547S XbaI,New England Biolabs,Cat# R0145S NotI,New England Biolabs,Cat# R0189S BsmbI,New England Biolabs,Cat#

(38) The complete Kaposi sarcoma-associated herpesvirus genome induces early-onset, metastatic angiosarcoma in transgenic miceCell host & microbeMay 8, 2024Sin SH, Eason AB, Kim Y, Schneider JW, Damania B, Dittmer DP.AB_2533552 "Chemicals, peptides, and recombinant proteins",, PacI restriction enzyme,New England Biolabs,Cat#R0547 Valganciclovir hydrochloride,Avachem Scientific,Cat#2602S Critical commercial assays,, Wizard SV Genomic DNA Purification System

(39) A novel in vivo system to study coral biomineralization in the starlet sea anemone, Nematostella vectensisiScienceMarch 15, 2024Brent Foster, Fredrik Hugosson, Federica Scucchia, Camille Enjolras, Leslie S. Babonis, William Hoaen, Mark Q. Martindaleactivity, DNA fragments were cloned into the pNvT-MHCmCherry vector (RRID:Addgene_67943) using PacI (NEB #R0547) and AscI (NEB #R0558) sites, thereby replacing the myosin heavy chain (MHC) promoter. Confirmed plasmids

(40) Familial Alzheimer mutations stabilize synaptotoxic γ-secretase-substrate complexesCell reportsFebruary 27, 2024Devkota S, Zhou R, Nagarajan V, Maesako M, Do H, Noorani A, Overmeyer C, Bhattarai S, Douglas JT, Saraf A, et al.CLM-1396 15NH4Cl,Cambridge Isotope Laboratories,Cat. No. NLM-467 Pac1 restriction enzyme,New England Biolabs,Cat. No. R0547S Swa1 restriction enzyme,New England Biolabs,Cat. No. R0604S BamH1 restriction enzyme,New England Biolabs,Cat.

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PacI

PacI from New England Biolabs

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PacI from New England Biolabs

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