PacI from New England Biolabs

Product Specs
ItemPacI
CompanyNew England Biolabs
Price $76.00 Inquire
Catalog NumberR0547S
Concentration4 u/µL
Quantity250 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

TTAAT/TAA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(41) Hepatocyte Deubiquitinating Enzyme OTUD5 Deficiency is a Key Aggravator for Metabolic Dysfunction-Associated Steatohepatitis by Disturbing Mitochondrial HomeostasisCellular and Molecular Gastroenterology and HepatologyJanuary 1, 2024Jingjing Dai, Liren Zhang, Ruizhi Zhang, Jing Ge, Feifan Yao, Suiqing Zhou, Jiali Xu, Kai Yu, Jing Xu, Longfeng Jiang, et al.VDAC2-overexpressing, or OTUD5-knockdown adenoviral vectors. The recombinant adenoviral vector was linearized by PacI (R0547L; New England Biolabs, Ipswich, MA) before co-transfection into 293 cells with polyethyleneimine (24765-1;

(42) A secreted form of chorismate mutase (Rv1885c) in Mycobacterium bovis BCG contributes to pathogenesis by inhibiting mitochondria-mediated apoptotic cell death of macrophagesJ Biomed SciDecember 18, 2023Mi-Hyun Lee, Hye Lin Kim, Hyejun Seo, Sangkwon Jung, Bum-Joon Kiminstructions. To introduce the selection markers that were needed to select mutants, the PacI (NEB, R0547L)-digested 8-kbp fragment of pGOAL19 was ligated with the p2NIL construct in which the TBCM flanking

(43) Generation and application of endogenously floxed alleles for cell-specific knockout in zebrafishDevelopmental CellNovember 1, 2023Masahiro Shin, Hui-Min Yin, Yu-Huan Shih, Takayuki Nozaki, Daneal Portman, Benjamin Toles, Amy Kolb, Kevin Luk, Sumio Isogai, Kinji Ishida, et al.,New England Biolabs,R3122 BstEII-HF,New England Biolabs,R3162 NcoI-HF,New England Biolabs,R3193 PacI,New England Biolabs,R0547 SspI-HF,New England Biolabs,R0132 BbsI-HF,New England Biolabs,R3539 BclI-HF,New England Biolabs,R3160 MscI,New England Biolabs

(44) Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degenerationiScienceOctober 20, 2023Selena Wei-Zhang, Bohao Cui, Man Xing, Jiaojiao Liu, Yingying Guo, Kai He, Tinghui Bai, Xue Dong, Yi Lei, Wei Zhou, et al.,New England Biolabs,Cat#R0629S PI-SceI,New England Biolabs,Cat#R0696S I-CeuI,New England Biolabs,Cat#R0699S PacI,New England Biolabs,Cat#R0547L ApaI,New England Biolabs,Cat#R0114S MfeI-HF,New England Biolabs,Cat#R3589L XhoI,New England Biolabs,Cat#R0146S 2×RealStar

(45) Versatile generation of precise gene edits in bovines using SEGCPNBMC BiologyOctober 20, 2023Ming Wang, Fangrong Ding, Haiping Wang, Ling Li, Yunping Dai, ZhaoLin Sun, Ning Lisynthesized by Sangon Biotech, and the product was cloned into the pCAG-stop2-EGFP vector by using PacI (R0547S, NEB). To construct the universal gene-targeting vector including the ESSEE, the loxP-TmOCT4-Cre-pA-POlII-Neo-pA-loxp

(46) RING finger protein 13 protects against nonalcoholic steatohepatitis by targeting STING-relayed signaling pathwaysNature CommunicationsOctober 20, 2023Zhibin Lin, Peijun Yang, Yufeng Hu, Hao Xu, Juanli Duan, Fei He, Kefeng Dou, Lin WangAdenoviral Expression System (V493-20; Invitrogen; Carlsbad, CA, USA) were used. After linearized by PacI (R0547L; NEB; MA, USA), the recombinant adenoviral vector was transfection into 293 cells with polyethyleneimine

(47) Microhomology-mediated circular DNA formation from oligonucleosomal fragments during spermatogenesiseLifeOctober 17, 2023Jun Hu, Zhe Zhang, Sai Xiao, Yalei Cao, Yinghong Chen, Jiaming Weng, Hui Jiang, Wei Li, Jia-Yu Chen, Chao Liubuffer (10 mM Tris-Cl, pH 8.0; 1 mM EDTA, pH 8.0) and treated with AsiSI (NEB, R0630S) and PacI (NEB, R0547S) endonucleases at 37°C for genomic DNA and mtDNA fragmentation. The remaining linear DNA was treated

(48) The FoxO1-ATGL axis alters milk lipolysis homeostasis through PI3K/AKT signaling pathway in dairy goat mammary epithelial cellsJournal of Animal ScienceAugust 28, 2023Qiuya He, Liangjiahui GaoLiang, Fuhong Zhang, Weiwei Yao, Jiao Wu, Ning Song, Jun Luo, Yong Zhangcells for recombination with pAdEasy-1 vector. The recombinant plasmids were linearized with PacI (R0547, NEB, Ipswich, MA, USA) and transfected into 293A cell lines to generate Ad-FoxO1 or Ad-GFP adenoviruses.

(49) Investigating Mitotic Inheritance of Histone Modifications Using Tethering StrategiesHistone MethyltransferasesJune 20, 2023Ajay Larkin, Amanda Ames, Melissa Seman, Kaushik Ragunathantagging vector for subcloning), BglII restriction enzyme (NEB R0144). PacI restriction enzyme (NEB R0547). AscI restriction enzyme (NEB R0558). Custom plasmid, containing binding site: specific example

(50) Development of virus-like particles with inbuilt immunostimulatory properties as vaccine candidatesFrontiers in MicrobiologyJune 7, 2023Simon Collett, Linda Earnest, Julio Carrera Montoya, Melissa A. Edeling, Ashley Yap, Chinn Yi Wong, Dale Christiansen, Jason Roberts, Jamie Mumford, Valerie Lecouturier, et al.Genome Research Facility (AGRF, WEHI). Digestion of the selected plasmid was then set up with PacI (R0547S, NEB, United States), ethanol precipitated and quantitated for transfection into 293A cells. DEN VLPs

(51) Enhanced fluorescent imaging of proteins in live yeast cells using fluorescently labeled scFvSTAR ProtocolsJune 2, 2023Ioannis Tsirkas, Tomer Zur, Daniel Dovrat, Zohar Paleiov, Lior Ravkaie, Amir Aharoni7732-18-5 SrfI + rCutsmart,New England Biolabs,Cat# R0629S PacI + rCutsmart,New England Biolabs,Cat# R0547S Agarose (powder),Life Gene,Cat# LAG0701 SYBR Safe DNA Gel Stain,Invitrogen,Cat# S33102 DNA Ladder

(52) HPV integration generates a cellular super-enhancer which functions as ecDNA to regulate genome-wide transcriptionNucleic Acids ResearchMay 22, 2023Rui Tian, Zheying Huang, Lifang Li, Jingping Yuan, Qinghua Zhang, Lirong Meng, Bin Lang, Yongfeng Hong, Chaoyue Zhong, Xun Tian, et al.remaining DNA was further digested at 37°C for 16 h using 2 U of rare-cutting endonuclease PacI (NEB, R0547) to remove the mitochondrial circular DNA. The remaining circular DNA was amplified using the φ29 DNA

(53) Lysosomal-associated protein transmembrane 5 ameliorates non-alcoholic steatohepatitis by promoting the degradation of CDC42 in miceNature communicationsMay 8, 2023Jiang L, Zhao J, Yang Q, Li M, Liu H, Xiao X, Tian S, Hu S, Liu Z, Yang P, et al.were used to generate mouse LAPTM5-overexpressing adenoviral vectors. After linearization with Pacl (R0547L; NEB, MA, USA), the adenoviral vectors were transfected into 293 cells using polyethyleneimine (24765-1;

(54) Optimized metrics for orthogonal combinatorial CRISPR screensScientific reportsMay 6, 2023Cetin R, Wegner M, Luwisch L, Saud S, Achmedov T, Süsser S, Vera-Guapi A, Müller K, Matthess Y, Quandt E, et al.manufacturer's protocol. 3 µg of pre-library was digested with PacI and I-SceI (New England Biolabs, R0547S and R0694S) restriction enzymes to remove the residual template plasmid from the library which has recognition

(55) Overexpression of Krüppel-Like Factor 9 Enhances the Antitumor Properties of Paclitaxel in Malignant Melanoma-Derived Cell LinesPharmaceuticals (Basel)April 6, 2023Mohammed O. Altonsy, George X. Song-Zhao, Mahmoud M. Mostafa, Paule Régine Mydlarskirecombination of the two vectors. Recombinant pAd-KLF9 vector was then linearized using PacI (catalog no. R0547S; New England Biolabs) and transfected in HEK293A (catalog no. R70507; Thermo, Markham, ON, Canada) cells

(56) The pluripotency factor Tex10 finetunes Wnt signaling for PGC and male germline developmentbioRxivFebruary 28, 2023Dan Li, Jihong Yang, Fanglin Ma, Vikas Malik, Ruge Zang, Xianle Shi, Xin Huang, Hongwei Zhou, Jianlong Wangmodified pJet1.2 plasmid were digested with the restriction enzymes AgeI (NEB, R0552S) and PacI (NEB, R0547S) and then ligated using T4 DNA ligase (NEB, M0202S) to obtain the plasmid pAW63-Tex10. The Tex10 guide

(57) Human JAK1 gain of function causes dysregulated myelopoeisis and severe allergic inflammationJCI insightDecember 22, 2022Biggs CM, Cordeiro-Santanach A, Prykhozhij SV, Deveau AP, Lin Y, Del Bel KL, Orben F, Ragotte RJ, Saferali A, Mostafavi S, et al.these PCR products into pCS2+MCS-P2A-sfGFP vector (Addgene, 74668) using digestion with PacI (NEB, R0547S) and AscI (NEB, R0558S) enzymes and standard cloning techniques. The resulting pCS2+JAK1-WT-P2A-sfGFP

(58) Use of a Bacterial Artificial Chromosome to Generate Recombinant SARS-CoV-2 Expressing Robust Levels of Reporter GenesMicrobiology SpectrumDecember 21, 2022Chengjin Ye, Luis Martinez-SobridoOpti-MEM (Thermo Fisher Scientific, catalog no. 31985-070), PacI (New England Biolabs, catalog no. R0547L), PciI (New England Biolabs, catalog no. R0655L), PCR cleanup system (Promega, catalog no. A9285), plasmid

(59) The mechanism of replication stalling and recovery within repetitive DNANature CommunicationsDecember 1, 2022Corella S. Casas-Delucchi, Manuel Daza-Martin, Sophie L. Williams, Gideon Costerrepetitive sequence. Briefly, 100 ng of plasmid was digested with 0.5U of NotI (NEB, R0189L) and PacI (NEB, R0547L) in 1× Cutsmart buffer (NEB, B7204S) at 37 °C for 30 min. Reactions were stopped by adding 50 mM EDTA.

(60) Systematic decomposition of sequence determinants governing CRISPR/Cas9 specificityNature CommunicationsDecember 1, 2022Rongjie Fu, Wei He, Jinzhuang Dou, Oscar D. Villarreal, Ella Bedford, Helen Wang, Connie Hou, Liang Zhang, Yalong Wang, Dacheng Ma, et al.Q5 site-directed mutagenesis (NEB #E0552). The resulting plasmids were then linearized by PacI (NEB #R0547), and undergone Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, NEB #E2621) with the amplified

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PacI

PacI from New England Biolabs

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PacI from New England Biolabs

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