Description
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Enhanced incorporation of modified nucleotides
- Single base incorporation of dideoxy and acyclonucleotides
Description:
Therminator™ DNA Polymerase is a 9°N™ DNA Polymerase variant with an enhanced ability to incorporate modified substrates such as dideoxynucleotides, ribonucleotides and acyclonucleotides
Source:
An
E. coli strain that carries the 9°N A485L (exo-) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase from
Thermococcus species 9°N-7.
Applications:
- DNA sequencing by partial ribosubstitution
- DNA sequencing using dideoxy or acyclo chain terminators
- SNP analysis with dideoxy or acyclo chain terminators
Reagents Supplied:
ThermoPol Reaction Buffer (10X)