ImProm-II Reverse Transcriptase from Promega

Product Specs
ItemImProm-II Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3802
Quantity100 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) WWP1 gain-of-function drives developmental anoikis through TGFβ pathway during neurodevelopmentCell Death DiscoveryMarch 6, 2026Ki Hurn So, Seungbok Lee, Jiayi Wong, Hyunsik Lee, Eun-Jin Yun, Se Song Jang, Hee-Jung Choi, Jong-Hee Chae, Seung Tae Baekusing LunaScript reverse transcriptase (NEB, #E3010L) or ImProm-II™ Reverse Transcriptase (Promega, #A3802). qPCR experiments were conducted with Luna Universal SYBR reagent (NEB, #M3003L) on a MIC qPCR Cycler

(2) 1,1-Diethoxyethane enhances aerobic respiration in human mitochondria via activation of AMP-activated protein kinaseCommun BiolMarch 3, 2026Thang Nguyen Huu, Hien Duong Thanh, Min-Kyu Kim, Dhiraj Kumar Sah, Vu Hoang Trinh, Hyun Joong Yoon, Jin Myung Choi, Geun-Haeng Lee, Seon-Young Kim, Seung-Rock Leeindicating purity. Complementary DNA (cDNA) was synthesized using Improm-IITM Reverse Transcriptase (A3802; Promega, Madison, WI, USA) following the manufacturer’s protocol. Semiquantitative PCR was performed

(3) IRF2 deficiency disrupts pyroptosis, NK cell interferon-γ production and resistance to FrancisellaEMBO ReportsJanuary 28, 2026Maxence Cornut, Sophia Djebali, Elena Rondeau, Sarah Dayet, Théo Fayolle, Julie Haagen, Lucie Fallone, Noémi Rousseaux, Emmanuelle Caspar, Mélissa Marcotte, et al.ELISA,R&D,DY410 ProcartaPlex Cytokine Panel,Thermo Fisher,PPX-15 ImProm-II Reverse Transcriptase,Promega,A3802 FastStart Universal SYBR Green Master (Rox),Roche,3736468 Reagents and tools table

(4) Genotype‐Specific Small EVs Released by Giardia lamblia Act as Mediators of Phenotypic Adaptation Under Metronidazole‐Induced StressJournal of Extracellular VesiclesSeptember 1, 2025Gabriel Luna Pizarro, Jerónimo Laiolo, Nehuén Salas, Rocío G. Patolsky, Luciano Díaz Pérez, Camilo Cotelo, Constanza Feliziani, Andrea Silvana Rópolo, María Carolina Touzprotocol. Two micrograms of total RNA were reverse‐transcribed using M‐MLV Reverse Transcriptase (Cat. No. A3802, Promega). The cDNA was analysed for the pyruvate oxidoreductase (PFOR) (gene ID GL50803_114609), Nitroreductase‐1

(5) Titin is a new factor regulating arterial stiffness through vascular smooth muscle cell tone in male ratsPhysiol RepMarch 22, 2025Chaoqun Zhu, Terrance Bishop, Zachery R. Gregorich, Wei GuoDNA contamination. 1 μg of total RNA was reverse transcribed with the ImProm‐II™ Reverse Transcriptase System (cat# A3802; Promega) using random primers. The reaction was used as a template for PCR using Taq DNA polymerase

(6) IFN-γ licenses normal and pathogenic ALPK1/TIFA pathway in human monocytesiScienceJanuary 17, 2025Amandine Martin, Solène Caron, Mélissa Marcotte, Pauline Bronnec, Etienne Garneret, Nora Martel, Georgina Maalouf, Pascal Sève, David Saadoun, Yvan Jamilloux, et al.site-directed,Agilent,Cat#200522 Nucleospin RNA kit,Macherey Nagel,Cat#740955 ImProm-II™ Reverse Transcription System,Promega,Cat#A3802 FastStart Universal SYBR Green Master Mix,Sigma-Aldrich,Cat#4913914001 BD Cytofix/Cytoperm™ Fixation/Permeabilization

(7) Cognitive impairment caused by compromised hepatic ketogenesis is prevented by endurance exerciseThe Journal of PhysiologyJanuary 14, 2025Taylor J. Kelty, Nathan R. Kerr, Chih H. Chou, Grace E. Shryack, Christopher L. Taylor, Alexa A. Krause, Alexandra R. Knutson, Josh Bunten, Tom E. Childs, Grace M. Meers, et al.as per the manufacturer’s instructions (Qiagen #74104) and a cDNA library was synthesized (Promega #A3802. Madison, WI, USA). A Nanodrop spectrometer was used to ensure RNA and cDNA purity. Quantitative real-time PCR

(8) Protein profile changes during priming explants to embryogenic response in Coffea canephora: identification of the RPN12 proteasome subunit involved in the protein degradationPeerJNovember 11, 2024Evelyn A. Carrillo-Bermejo, Ligia Brito-Argáez, Rosa M. Galaz-Ávalos, Felipe Barredo-Pool, Víctor M. Loyola-Vargas, Victor Aguilar-Hernándezμg of RNA was used to synthesize cDNA with 200 units of the ImProm-II™ reverse transcriptase (Cat # A3802; Promega, Madison, WI, USA) and 0.5 μg oligo dT18 primers (Cat # SO131; Thermo Fisher Scientific, Waltham,

(9) Modified mRNA-Mediated CCN5 Gene Transfer Ameliorates Cardiac Dysfunction and Fibrosis without Adverse Structural RemodelingInternational journal of molecular sciencesJune 6, 2024Song MH, Yoo J, Kwon DA, Chepurko E, Cho S, Fargnoli A, Hajjar RJ, Park WJ, Zangi L, Jeong D.RNA purification kit (Qiagen, Hilden, Germany, #74004). cDNA was synthesized using ImProm-II reverse transcriptase (#A3802; Promega, Madison, WI, USA) with oligo-dT priming. PCR was performed using a Thermal Cycle Dice Real-Time

(10) Activation of endogenous retroviruses and induction of viral mimicry by MEK1/2 inhibition in pancreatic cancerScience advancesMarch 29, 2024Cortesi A, Gandolfi F, Arco F, Di Chiaro P, Valli E, Polletti S, Noberini R, Gualdrini F, Attanasio S, Citron F, et al.R2062), and 100 to 500 ng were reverse-transcribed using ImProm-II Reverse Transcriptase kit (Promega, A3802). For the amplification of repetitive elements, a reaction control was assembled, which did not contain

(11) IGFBP3 promotes resistance to Olaparib via modulating EGFR signaling in advanced prostate canceriScienceFebruary 16, 2024Leslie AR, Ning S, Armstrong CM, D'Abronzo LS, Sharifi M, Schaaf ZA, Lou W, Liu C, Evans CP, Lombard AP, et al.Scientific (Milton Park, UK). The cDNA was prepared using ImProm-II reverse transcriptase (Cat#: PR-A3802) purchased from Promega (Madison, WI, USA). The cDNAs were subjected to quantitative PCR (qPCR) using

(12) Western diet-induced obesity results in brain mitochondrial dysfunction in female Ossabaw swineFront Mol NeurosciDecember 14, 2023Taylor J. Kelty, Chris L. Taylor, Nicole E. Wieschhaus, Pamela K. Thorne, Amira R. Amin, Christina M. Mueller, T. Dylan Olver, Darla L. Tharp, Craig A. Emter, Alexander W. Caulk, et al.per manufacturer’s instructions (Qiagen, Cat#74104) and a cDNA library was synthesized (Promega, Cat#A3802). A nanodrop spectrometer was used to assess RNA and cDNA purity. Quantitative real-time PCR (qRT-PCR)

(13) Defining diurnal fluctuations in mouse choroid plexus and CSF at high molecular, spatial, and temporal resolutionNature CommunicationsJune 22, 2023Ryann M. Fame, Peter N. Kalugin, Boryana Petrova, Huixin Xu, Paul A. Soden, Frederick B. Shipley, Neil Dani, Bradford Grant, Aja Pragana, Joshua P. Head, et al.reverse-transcribed into cDNA using the Promega™ ImProm-II™ Reverse Transcription System (Fisher PR-A3802) following manufacturer’s specifications with random hexamer primers (Promega PR-C1181). RT-qPCRs were

(14) Primary cilia sense glutamine availability and respond via asparagine synthetaseNature metabolismMarch 1, 2023Steidl ME, Nigro EA, Nielsen AK, Pagliarini R, Cassina L, Lampis M, Podrini C, Chiaravalli M, Mannella V, Distefano G, et al.(Promega, #C1101) or Random Primers (Promega, #C1181) and ImProm-II Reverse Transcriptase (Promega, #A3802). Quantitative real-time PCR analysis was performed on technical duplicates using iTaq Univer SYBR Green

(15) Transcriptional expression of CXCL10 and STAT1 in lupus nephritis and the intervention effect of triptolideClinical rheumatologyFebruary 1, 2023Shi D, Li Y, Shi X, Yao M, Wu D, Zheng Y, Lin Q, Yang Y.from Japan Tongren Institute of Chemistry. The RNA extraction kit(#LS1040), reverse transcription kit(#A3802), and real-time fluorescence quantitative PCR(#A1250) kit were purchased from Promega Company. The upstream

(16) Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibitionNeuronJuly 6, 2022Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, Bischofberger J, Scheiffele P.Oligo(dT)15 primer (Promega C1101) for flanking primer analysis and ImProm II reverse transcriptase (Promega A3802). For qPCR assays, two technical replicates were run per experiment and the mean was calculated. The

(17) Repurposing of FDA Approved Drugs Against SARS-CoV-2 Papain-Like Protease: Computational, Biochemical, and in vitro StudiesFrontiers in MicrobiologyMay 10, 2022Rajkumar Kulandaisamy et al.per manufacturer's instructions to synthesise the cDNA using ImProm-II Reverse Transcriptase (Cat # A3802, Promega Corporation, India). One-fourth of the cDNA preparation and the primer set, MBA134 (Forward)

(18) Impairment of Glucose Metabolism and Suppression of Stemness in MCF-7/SC Human Breast Cancer Stem Cells by NootkatonePharmaceuticsApril 21, 2022Yen Thi-Kim Nguyen, Ngoc Bao To, Vi Nguyen-Phuong Truong, Hee Young Kim, Meran Keshawa Ediriweera, Yoongho Lim, Somi Kim Cho(Invitrogen) reagent and reversed-transcribed into cDNA using ImProm-IITM Reverse Transcriptase (cat. no. A3802; Promega, Madison, WI, USA) following the manufacturer’s instructions. Then, two-step quantitative real-time PCR

(19) Spatiotemporal analysis identifies ABF2 and ABF3 as key hubs of endodermal response to nitrateProceedings of the National Academy of SciencesJanuary 25, 2022Orlando Contreras-López et al.using ImProm-II Reverse Transcription System according to the instruction of the manufacturer (Catalog #A3802, Promega). qRT-PCR was carried out using the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix following

(20) Kaposi's sarcoma herpesvirus activates the hypoxia response to usurp HIF2α-dependent translation initiation for replication and oncogenesisCell reportsDecember 28, 2021Méndez-Solís O, Bendjennat M, Naipauer J, Theodoridis PR, Ho JJD, Verdun RE, Hare JM, Cesarman E, Lee S, Mesri EA.79254 Amplification grade DNase I,Sigma,Cat.# AMPD1 Im-PromII Reverse Transcriptase,Promega,Cat.# A3802 Lipofectamine 2000,Life Technologies,Cat.# 11668-027 Lipofectamine RNAiMAX reagent,Life Technologies,Cat.#

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