ImProm-II Reverse Transcriptase from Promega

Product Specs
ItemImProm-II Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3801
Quantity10 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Ex vivo and in vivo CRISPR/Cas9 screenings identify the roles of protein N-glycosylation in regulating T-cell activation and functionseLifeMarch 20, 2026Yu Hong, Xiaofang Si, Wenjing Liu, Xueying Mai, Yu Zhang(Ambion, Cat# 15596018). cDNA was synthesized by the ImProm-II Reverse Transcriptase system (Promega, Cat# A3801) using 100 ng RNA per reaction. The qPCR reactions were prepared with TB Green Premix Ex Taq (Takara,

(2) Personality-based intergenerational effects of prenatal THC exposure in an inherited mouse model of social dominance and submissivenessSci RepAugust 20, 2025Mohamed Mari, Anastasia Bagaev, Debpali Sur, Beatriz G. S. Rocha, Dilorom Begmatova, Natalia Zemliana, Abdalla Bowirrat, Kenneth Blum, Panayotis K. Thanos, Natalya M. Kogan, et al.spectrophotometer. Reverse transcription was performed using the GoScript™ Reverse Transcription System (Promega, A3801, Madison, WI) according to the manufacturer’s instructions. Quantitative real-time PCR (qRT-PCR) was

(3) Maternal oral sodium propionate supplementation restores gut integrity and mitigates stress-induced metabolic and behavioral outcomes in offspringTransl PsychiatryJuly 9, 2025Anastasia Bagaev, Debpali Sur, Oryan Agranyoni, Naamah Pe'er, Brajesh Kumar Savita, Beatriz Gonçalves Silva Rocha, Panayotis K. Thanos, Shiri Navon-Venezia, Albert Pinhasov(Qiagen, USA, cat. 74004). Reverse transcription was performed with cDNA Kit GoScript™ (Promega, USA, cat. A3801). RT-PCR was performed using SYBR™ Green PCR Master Mix (Applied Biosystems, USA, cat. 4309155) on the

(4) Differentiating Peromyscus leucopus bone marrow-derived macrophages for characterization of responses to Borrelia burgdorferi and lipopolysaccharideInfection and immunityJuly 8, 2025Wells CC, Petnicki-Ocwieja T, Tan S, Bunnell SC, Telford SR 3rd, Hu LT, Bourgeois JS.217084). cDNA was synthesized from isolated RNA using the ImProm-II Reverse Transcriptase kit (Promega A3801). qPCR was completed using the iTaq Universal SYBR Green Supermix (Biorad 1725124). Reactions were performed

(5) The XPO1 Inhibitor Eltanexor Modulates the Wnt/β-Catenin Signaling Pathway to Reduce Colorectal Cancer TumorigenesisCancer Res CommunJuly 2, 2025Andrew E. Evans, Sahida Afroz, Alexa Magstadt, Anup Kasi, Dan A. Dixon1 μg of total RNA in combination with olgio(dT) and ImProm-II Reverse Transcriptase (Promega, cat. #A3801). qPCR using the indicated primers (Table 1) was performed as previously described (20) with SYBR green PCR master mix

(6) The dsRNA-dependent kinase (PKR) inhibits the growth of Leishmania major via NF-κB-mediated genesParasitologyJuly 1, 2025Áislan de Carvalho Vivarini, Bianca Cristina Duarte Vivarini, Yuri Nunes Oliveira, Flavia Thiebaut, Evelize Folly das ChagasGermany). Ad 1 µg of the extracted RNA was then reverse-transcribed to first-strand cDNA using ImProm-II (#A3801 – Promega) and oligo(dT) primer, following the manufacturer’s instructions. The DNA sequences of the

(7) Nanopore ReCappable sequencing maps SARS-CoV-2 5′ capping sites and provides new insights into the structure of sgRNAsNucleic Acids ResearchApril 8, 2022Camilla Ugolini et al.(Qiagen, #1038703) and then retrotranscribed using the ImProm-II™ Reverse Transcription System (Promega, #A3801), according to the vendor's instructions. PCR products were separated on an agarose gel and the distinct

(8) Role of laminin and cognate receptors in cholangiocarcinoma cell migrationCell adhesion & migrationDecember 1, 2021Islam K, Thummarati P, Kaewkong P, Sripa B, Suthiphongchai T.

(9) SPX-related genes regulate phosphorus homeostasis in the marine phytoplankton, Phaeodactylum tricornutumCommunications BiologyDecember 1, 2021Kaidian Zhang, Zhi Zhou, Jiashun Li, Jingtian Wang, Liying Yu, Senjie Lin

(10) RBM20-Mediated Pre-mRNA Splicing Has Muscle-Specificity and Differential Hormonal Responses between Muscles and in Muscle Cell CulturesInternational Journal of Molecular SciencesMarch 13, 2021Rexiati Maimaiti, Chaoqun Zhu, Yanghai Zhang, Qiyue Ding, Wei Guogenomic DNA. Reverse transcriptase assay was conducted with ImProm-II reverse transcription system (#A3801, Promega, Madison, WI, USA). PCR was carried out using a C1000 thermal cycler. Primers for the amplification

(11) Reverse Transcriptase: From Transcriptomics to Genome EditingTrends in BiotechnologyFebruary 1, 2021Samara Martín-Alonso, Estrella Frutos-Beltrán, Luis Menéndez-Arias(https://www.qiagen.com/us/products/discovery‐and‐translational‐ research/pcr‐qpcr/real‐time‐pcr‐enzymes‐and‐kits/probe‐based‐ qpcr/quantinova‐pathogen‐ic‐kit/?clear=true#orderinginformation) ImProm™‐II (Promega) (1) Promega. (https://www.promega.es/products/pcr/rt‐pcr/improm_ii‐ reverse‐transcriptase/?catNum=A3801) (2) Lv, G. et al. (2014). MicroRNA‐451 regulates activating transcription factor 2 expression and inhibits

(12) Identification of a Signaling Mechanism by Which the Microbiome Regulates Th17 Cell-Mediated Depressive-Like Behaviors in MiceThe American journal of psychiatryOctober 1, 2020Medina-Rodriguez EM, Madorma D, O'Connor G, Mason BL, Han D, Deo SK, Oppenheimer M, Nemeroff CB, Trivedi MH, Daunert S, et al.

(13) MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cellsBiological researchSeptember 29, 2020Cao D, Zhu H, Zhao Q, Huang J, Zhou C, He J, Liang Y.RQ1 RNase-free DNase (M6101; Promega, Madison, WI, USA), cDNA was synthesized with the Prime-Script RT reagent kit (A3801; Promega, Madison, WI, USA) using the specific reaction system (Total RNA 0.625 μg in 4.25 μl, ImProm

(14) The transcription factor OsSUF4 interacts with SDG725 in promoting H3K36me3 establishmentNature CommunicationsDecember 1, 2019Bing Liu, Yuhao Liu, Baihui Wang, Qiang Luo, Jinlei Shi, Jianhua Gan, Wen-Hui Shen, Yu Yu, Aiwu Dongcollected under SD conditions. Reverse transcription was performed using Improm-II reverse transcriptase (A3801, Promega, Madison, WI, USA) according to the manufacturer’s protocol. Quantitative PCR was performed

(15) Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in miceGenome biologyNovember 8, 2018Duan J, Lu G, Hong Y, Hu Q, Mai X, Guo J, Si X, Wang F, Zhang Y.(Zymo Research, Cat#R2051). cDNA was synthesized by the ImProm-II™ Reverse Transcriptase system (Promega, Cat#A3801) using 100 ng of RNA per reaction. The qPCR reactions were prepared with the KAPA SYBR® FAST qPCR Kit

(16) Integrating Rio1 activities discloses its nutrient-activated network in Saccharomyces cerevisiaeNucleic acids researchSeptember 6, 2018Iacovella MG, Bremang M, Basha O, Giacò L, Carotenuto W, Golfieri C, Szakal B, Dal Maschio M, Infantino V, Beznoussenko GV, et al.indicated above, was reverse transcribed into cDNA with ImProm-II reverse transcriptase (Promega, Cat # A3801) and random primers (Life Technologies, Cat # 48190-011). A total of 5 ng of the cDNA were then submitted

(17) In Planta Preliminary Screening of ER Glycoprotein Folding Quality Control (ERQC) ModulatorsInternational Journal of Molecular SciencesJuly 23, 2018Lucia Marti, Andrea Lia, Ida-Barbara Reca, Pietro Roversi, Angelo Santino, Nicole Zitzmann

(18) Identification of Ice Plant (Mesembryanthemum crystallinum L.) MicroRNAs Using RNA-Seq and Their Putative Roles in High Salinity Responses in SeedlingsFrontiers in Plant ScienceAugust 9, 2016Chih-Pin Chiang, Won C. Yim, Ying-Hsuan Sun, Miwa Ohnishi, Tetsuro Mimura, John C. Cushman, Hungchen E. Yen

(19) Short Communication An efficient method for simultaneous extraction of high-quality RNA and DNA from various plant tissuesGenetics and Molecular ResearchDecember 29, 2015R.R. Oliveira, A.J.C. Viana, A.C.E. Reátegui, M.G.A. Vincentzwas synthesized by reverse transcription with the kit ImPromII (Promega, Madison, WI, USA, cat. No. #A3801), using oligo-dT as a primer. For qRT-PCR, 0.5 μL cDNA was used as a template together with forward

(20) Regulation of Human Adenovirus Replication by RNA InterferenceActa NaturaeOctober 21, 2015N. A. Nikitenko, T. Speiseder, E. Lam, P. M. Rubtsov, Kh. D. Tonaeva, S. A. Borzenok, T. Dobner, V. S. Prassolov

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