ImProm-II Reverse Transcriptase from Promega

Product Specs
ItemImProm-II Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3803
Quantity500 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccinesMol Ther Nucleic AcidsJune 16, 2026Han Young Seo, Haewon Jung, Se-Young Lee, Hae-Gwang Jung, Hee Cho, Yu-Min Son, Yeonju Bak, Seo-Yeon Hwang, Jung-Hee Kim, In Ho Park, et al.NM_004095.4) was performed using ImProm-II Reverse Transcriptase (Promega, Madison, WI, USA; cat. no. A3803) and a reverse primer 5′-TTA AAT GTC CAT CTC AAA CTG TGA CTC TTC-3′. The resulting cDNA encoding 4E-BP1

(2) ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinomaCell DiscovApril 21, 2026Lijie Han, Lina Gan, Balázs Schäfer, Dillon M. Voss, Mathias Danielsen, Ondrej Kostov, Jia Liu, Ting Wang, Marvin H. Caruthers, Hao Chen, et al.manufacturer’s protocol. RT-PCR was subsequently performed using ImProm-II Reverse Transcriptase (Promega, A3803) according to the manufacturer’s protocol. Radioactive PCR was conducted using 32P-α-dCTP and 1.25 U

(3) Flavivirus NS1-triggered endothelial dysfunction promotes virus disseminationPLOS PathogensDecember 31, 2025Henry Puerta-Guardo, Scott B. Biering, Bryan Castillo-Rojas, Michael J. DiBiasio-White, Nicholas T. N. Lo, Diego A. Espinosa, Colin M. Warnes, Chunling Wang, Thu M. Cao, Dustin R. Glasner, et al.cDNA was reverse-transcribed from 1 µg of total RNA using IMPROM-II reverse transcriptase (Promega, A3803) with a random hexamer primer according to the manufacturer’s instructions. qPCR was conducted using

(4) PR inhibition stimulates G6PD expression to enhance malignancy in luminal breast cancerCell Death & DiseaseDecember 21, 2025Jae Woong Jeong, Janghee Lee, Soong June Bae, Yoon Jin Cha, Sungsoon Fang, Hae-kyung Lee, Sung Gwe Ahnprotocol. Complementary DNA (cDNA) was established using ImProm-II Reverse Transcriptase (Promega, A3803). Real-time qPCR was performed using TOP Real qPCR 2X Pre-MIX (Enzynomics, RT501S) with specific primers

(5) Depletion of myeloid cells in AG129 mice reduces the infection-mediated oxidative stress and restrains dengue virus-induced thymic atrophyCell Communication and SignalingDecember 6, 2025B. Kusuma, Surender Rawat, Oindrila Saha, Sakshi Nimesh, Srikanth Sadhu, Amit Awasthi, Tushar K. Maiti, Arup Banerjeeµg of total RNA was then reverse-transcribed to cDNA using the Promega cDNA synthesis kit (Promega, #A3803)). Diluted cDNAs to a final concentration of 100 ng were used for qPCR by using SYBR green DNA-binding

(6) Presenilin 2 regulates corticosterone-induced autophagic death of adult hippocampal neural stem cellsAnim Cells Syst (Seoul)December 4, 2025Jihyun Hong, Hyun-Kyu An, Hyeri Nam, Jieun Choi, Seong-Woon Yu(Sigma-Aldrich, 2432) and reverse-transcribed into cDNA using ImProm-II Reverse Transcriptase kit (Promega, A3803). Real-time qPCR was performed with TOPrealTM qPCR 2X PreMIX (Enzynomics, RT500M) in the CFX96 Real-Time

(7) TLR9 signaling requires ligand-induced phosphorylation of two specific tyrosine residues by EGFR and SykmBioNovember 12, 2025Manoj Veleeparambil, Chenyao Wang, Patricia M. Kessler, Pracheta Sengupta, Santanu Das, Ritu Chakravarti, Belinda Willard, Ganes C. Sen, Saurabh Chattopadhyaykit (11828665001, Roche), and used for cDNA preparation using Impromp-II Reverse Transcription Kit (A3803, Promega). The cDNA (0.5 ng) was applied to a 384-well plate for real-time PCR using SYBR Green PCR

(8) Mutant huntingtin induces neuronal apoptosis via derepressing the non-canonical poly(A) polymerase PAPD5Nat CommunApril 9, 2025Zhefan Stephen Chen, Shaohong Isaac Peng, Lok I Leong, Terence Gall-Duncan, Nathan Siu Jun Wong, Tsz Ho Li, Xiao Lin, Yuming Wei, Alex Chun Koon, Junzhe Huang, et al.respectively. The reverse transcription (RT) was performed using the ImProm-IITM Reverse Transcription System (A3803, Promega) according to the manufacturer’s instructions. The TB Green master mix (RR420A, Takara Bio Inc

(9) Homeostatic antiviral protection of the neonatal gut epithelium by interferon lambdaCell ReportsFebruary 25, 2025Bryan Ramirez Ramirez Reyes, Shelby Madden, Kimberly A. Meyer, Brenden Bartsch, Austin P. Wright, David A. Constant, Timothy J. NiceR2050 TURBO DNA-free™ Kit,Thermo Fisher,Cat# AM1907 ImProm-II™ Reverse Transcriptase Kit,Promega,Cat# A3803 PerfeCTa qPCR FastMix II,Quantabio,Cat# 95119 PowerUp™ SYBR™ Green Master Mix for qPCR,Thermo Fisher,Cat#

(10) Ablation of PI3Kγ in neurons protects mice from diet-induced obesity MASLD and insulin resistanceiScienceJanuary 17, 2025Angela Molinaro, Arianna Mazzoli, Andrea Usseglio Gaudi, Amit Chand Gupta, Vagner Ramon Rodrigues Silva, Damien Ramel, Muriel Laffargue, Johan Ruud, Barbara Becattini, Giovanni Solinas"Hematoxylin Solution (Mayer's, Modified)",Abcam,AB220365 Eosin,Sigma,HT110116 Glucose strips,Contour Next,Ascensia ImProm-II™ Reverse Transcriptase,Promega,A3803 SsoAdvanced Universal SYBR® Green Supermix,Bio-Rad,1725270 ,, Deposited data,Deposited data,Deposited

(11) Comparative Analysis of the Therapeutic Effects of MSCs From Umbilical Cord, Bone Marrow, and Adipose Tissue and Investigating the Impact of Oxidized RNA on Radiation‐Induced Lung InjuryStem Cells InternationalOctober 24, 2024Rui Zhai, Fumin Tai, Kexin Ding, Xin Tan, Hujie Li, Zhengyue Cao, Changhui Ge, Xiaofei Zheng, Hanjiang Fuexperimental conditions, reverse transcription was performed using 0.1 μL ImProm-II Reverse Transcriptase (A3803, Promega, USA), which is 10% of the recommended dosage of enzyme, according to our experience. Experiment

(12) Tisochrysis lutea Fucoxanthin Suppresses NF-κB, JNK, and p38-Associated MMP Expression in Arthritis Pathogenesis via Antioxidant ActivityAntioxidants (Basel)August 2, 2024Hyemi Lee, Hahyeong Jang, Dahyoon Heo, Jae-In Eom, Cheol-Ho Han, Se-Min Kim, Yoo-Seob Shin, Cheol-Ho Pan, Siyoung Yangtotal RNA was converted into complementary DNA (cDNA) using an Im-Prom-IITM Reverse Transcriptase kit (A3803; Promega, Madison, WI, USA) and oligo-dT primers. The primer and PCR conditions used for each gene were

(13) Long non‑coding RNA lung cancer‑associated transcript 1 regulates ferroptosis via microRNA‑34a‑5p‑mediated GTP cyclohydrolase 1 downregulation in lung cancer cellsInternational Journal of OncologyMay 10, 2024Fumin Tai, Rui Zhai, Kexin Ding, Yaocang Zhang, Hexi Yang, Hujie Li, Qiong Wang, Zhengyue Cao, Changhui Ge, Hanjiang Fu, et al.RNA was reverse transcribed into cDNA using an ImProm-II™ Reverse Transcriptase Reagent Kit (cat. no. A3803; Promega Corporation) according to the manufacturer's instructions. qPCR was performed in a Mx3000P

(14) WGA-M001, a Mixture of Total Extracts of Tagetes erecta and Ocimum basilicum, Synergistically Alleviates Cartilage Destruction by Inhibiting ERK and NF-κB SignalingInt J Mol SciDecember 14, 2023Eunjeong Oh, Hahyeong Jang, Subin Ok, Jiwon Eom, Hyunyong Lee, Sung Hun Kim, Jong Hwa Kim, Yu Mi Jeong, Kyeong Jin Kim, Seung Pil Yun, et al.RNA was reverse transcribed and cDNA was synthesized using the ImProm-IITM Reverse Transcriptase kit (A3803; Promega, Madison, WI, USA) and oligo-dT primers. Primer and PCR conditions (annealing temperature and

(15) Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone lossExperimental & molecular medicineDecember 1, 2023Tran TT, Lee G, Huh YH, Chung KH, Lee SY, Park KH, Kwon SH, Kook MS, Chun JS, Koh JT, et al.The synthesis of cDNA was performed from 0.25 to 0.5 μg of RNA via reverse transcription (Promega, A3803) and subjected to PCR (Geneall, 501-025). The Supplementary Table lists the PCR primers and experimental

(16) Regulation of sexually dimorphic placental adaptation in LPS exposure-induced intrauterine growth restrictionMolecular MedicineSeptember 18, 2023Da Som Jeong, Ji-Yeon Lee, Myoung Hee Kim, Ji Hoon Ohperformed with 1 µg of total RNA using ImProm-II Reverse Transcriptase (Promega, Madison, WI, USA, A3803) and RNase inhibitor (Promega, N2515). For quantitative real-time polymerase chain reaction (RT-qPCR),

(17) Co-inhibition of glutaminolysis and one-carbon metabolism promotes ROS accumulation leading to enhancement of chemotherapeutic efficacy in anaplastic thyroid cancerCell Death & DiseaseAugust 12, 2023Yeseong Hwang, Hyeok Jun Yun, Jae Woong Jeong, Minki Kim, Seyeon Joo, Hae-Kyung Lee, Hang-Seok Chang, Seok-Mo Kim, Sungsoon Fang15596018). cDNA was synthesized using 1 ug total RNA and ImProm-II™ Reverse Transcriptase (Promega, A3803). Real-time RT-PCR was performed using TOP Real ™ qPCR 2X Pre-MIX (Enzynomics, RT501S) and specific

(18) RNA-Sequencing Reveals Upregulation and a Beneficial Role of Autophagy in Myoblast Differentiation and FusionCellsNovember 10, 2022Lyu P, Jiang H.(1620147) from BIO-RAD (Hercules, CA, USA). Random primers (C1181), ImProm-II Reverse Transcriptase (A3803), RNasin Ribonuclease Inhibitor (N2615), and Deoxyribonucleoside Triphosphate (dNTP) Mix (U1515) were

(19) Multifaceted investigation underlies diverse mechanisms contributing to the downregulation of Hedgehog pathway-associated genes INTU and IFT88 in lung adenocarcinoma and uterine corpus endometrial carcinomaAging (Albany NY)October 15, 2022Ho Yin Edwin Chan, Zhefan Stephen ChenThermo Fisher Scientific). The reverse transcription was performed using ImProm-II™ Reverse Transcription System (A3803, Promega) and random hexamer (N8080127, Thermo Fisher Scientific) according to the manufacturers’ instructions.

(20) Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progressioneLifeAugust 1, 2022Liu Q, Luo Q, Feng J, Zhao Y, Ma B, Cheng H, Zhao T, Lei H, Mu C, Chen L, et al.reagent (Sigma T9424), reverse transcription PCR was performed with a Reverse Transcriptase kit (Promega A3803), and real-time PCR was performed using Powerup SYBR Green PCR master mix (A25743) and a Step-One Plus

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