ImProm-II Reverse Transcriptase from Promega

Product Specs
ItemImProm-II Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA3803
Quantity500 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) High Iron Exposure from the Fetal Stage to Adulthood in Mice Alters Lipid MetabolismNutrientsJune 13, 2022Minju Kim, Yeon-hee Kim, Sohyun Min, Seung-Min Leesynthesize cDNA using a commercial ImProm-II Reverse Transcriptase kit (Promega, Madison, WI, USA; A3803) with a random primer mixture according to the manufacturer’s instructions. Quantitative real-time PCR

(22) Influence of incubation, diet, and sex on avian uncoupling protein expression and oxidative stress in market age broilers following exposure to acute heat stressPoultry ScienceMay 1, 2022Kelly E. Brannan, Kaylee K. Helfrich, George R. Flentke, Susan M. Smith, Kimberly A. Livingston, Christine Jansen van Rensburgperformed using 1 μg of RNA with random hexamer primers and ImProm-II Reverse Transcriptase (item no. A3803, Promega, Madison, WI) diluted to a concentration of 1 μg/μL, prepared and synthesized as per manufacturer's

(23) YAP/TAZ and ATF4 drive resistance to Sorafenib in hepatocellular carcinoma by preventing ferroptosisEMBO Molecular MedicineDecember 7, 2021Ruize Gao et al.reagent (Sigma T9424), reverse transcription PCR was performed using Reverse Transcriptase kit (Promega A3803), and real‐time PCR was performed using Powerup SYBR Green PCR master mix (A25743) on a Step‐One Plus

(24) Role of laminin and cognate receptors in cholangiocarcinoma cell migrationCell adhesion & migrationDecember 1, 2021Islam K, Thummarati P, Kaewkong P, Sripa B, Suthiphongchai T.

(25) SPX-related genes regulate phosphorus homeostasis in the marine phytoplankton, Phaeodactylum tricornutumCommunications BiologyDecember 1, 2021Kaidian Zhang, Zhi Zhou, Jiashun Li, Jingtian Wang, Liying Yu, Senjie Lin

(26) A PGE₂-MEF2A axis enables context-dependent control of inflammatory gene expressionImmunityAugust 10, 2021Cilenti F, Barbiera G, Caronni N, Iodice D, Montaldo E, Barresi S, Lusito E, Cuzzola V, Vittoria FM, Mezzanzanica L, et al.RLT,QIAGEN,79216 RNeasy Plus Micro kit,QIAGEN,74034 ImProm-II Reverse Transcription System,Promega,A3803 SuperScript IV VILO Master Mix,Thermo Fisher Scientific,11756050 CD14 MicroBeads,Miltenyi,130-050-201 GeneArt Precision gRNA synthesis kit

(27) USP29-mediated HIF1α stabilization is associated with Sorafenib resistance of hepatocellular carcinoma cells by upregulating glycolysisOncogenesisJuly 16, 2021Ruize Gao et al.reagent (Sigma T9424), reverse transcription PCR was performed with Reverse Transcriptase kit (Promega A3803), real-time PCR was performed using Powerup SYBR Green PCR master mix (A25743) and a Step-One Plus real-time PCR

(28) Obtusifolin, an Anthraquinone Extracted from Senna obtusifolia (L.) H.S.Irwin & Barneby, Reduces Inflammation in a Mouse Osteoarthritis ModelPharmaceuticals (Basel, Switzerland)March 10, 2021Nam J, Seol DW, Lee CG, Wee G, Yang S, Pan CH.was reverse transcribed and used to synthesize cDNA using the ImProm-II™ Reverse Transcriptase kit (A3803; Promega, Madison, WI, USA). The primers and temperature conditions used for each gene are summarized

(29) Hyperinsulinemia and insulin resistance in the obese may develop as part of a homeostatic response to elevated free fatty acids: A mechanistic case-control and a population-based cohort studyEBioMedicineMarch 9, 2021Emanuel Fryk et al.guanidinium–thiocyanate extraction method. cDNA was prepared using the ImProm-II™ Reverse Transcriptase (Promega, A3803), and qPCR was performed using the commercial SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad 172-5274).

(30) A fine balance between Prpf19 and Exoc7 in achieving degradation of aggregated protein and suppression of cell death in spinocerebellar ataxia type 3Cell death & diseaseFebruary 2, 2021Chen ZS, Huang X, Talbot K, Chan HYE.Thermo Fisher Scientific). The reverse transcription was performed using ImProm-II™ Reverse Transcription System (A3803, Promega, Madison, WI, USA) according to the manufacturer’s instructions. The PCR primers used were

(31) Dominant-negative NFKBIA mutation promotes IL-1β production causing hepatic disease with severe immunodeficiencyThe Journal of clinical investigationNovember 2, 2020Tan EE, Hopkins RA, Lim CK, Jamuar SS, Ong C, Thoon KC, Koh MJ, Shin EM, Lian DW, Weerasooriya M, et al.RNase-free water. RNA samples were converted to cDNA using ImProm-II Reverse Transcriptase Enzyme (A3803, Promega) and dNTP (U1515m, Promega), according to the manufacturer’s instructions, in a T100 Thermal Cycler

(32) Identification of a Signaling Mechanism by Which the Microbiome Regulates Th17 Cell-Mediated Depressive-Like Behaviors in MiceThe American journal of psychiatryOctober 1, 2020Medina-Rodriguez EM, Madorma D, O'Connor G, Mason BL, Han D, Deo SK, Oppenheimer M, Nemeroff CB, Trivedi MH, Daunert S, et al.

(33) Antisense oligonucleotide modulation of non-productive alternative splicing upregulates gene expressionNature communicationsJuly 9, 2020Lim KH, Han Z, Jeon HY, Kach J, Jing E, Weyn-Vanhentenryck S, Downs M, Corrionero A, Oh R, Scharner J, et al.absorbance. cDNA synthesis cDNA was synthesized using the ImProm-II reverse transcriptase kit (Promega, A3803). In all, 1 µg of the RNA template was mixed with 0.5 µg of Oligo dT in a total volume 11 µL. The mixture

(34) MTFMT deficiency correlates with reduced mitochondrial integrity and enhanced host susceptibility to intracellular infectionScientific reportsJuly 7, 2020Seo JH, Hwang CS, Yoo JY.cDNA synthesis was conducted using 500 ng of RNA with the ImProm-II Reverse Transcription System (Promega, cat. #A3803). To measure mRNA expression levels, quantitative PCR and real-time PCR were performed with a typical

(35) ID1-Mediated BMP Signaling Pathway Potentiates Glucagon-Like Peptide-1 Secretion in Response to Nutrient ReplenishmentInternational journal of molecular sciencesMay 28, 2020Jeong JW, Kim M, Lee J, Lee HK, Ko Y, Kim H, Fang S.manufacturer’s protocol. cDNA was synthesized from 1 µg of total RNA using ImProm-II Reverse Transcriptase (A3803, Promega at Madison, WI, USA). Quantitative PCR (qPCR) was performed using TOPreal qPCR 2X PreMIX (SYBR

(36) A crystal structure of a collaborative RNA regulatory complex reveals mechanisms to refine target specificityeLifeAugust 9, 2019Chen Qiu, Vandita D Bhat, Sanjana Rajeev, Chi Zhang, Alexa E Lasley, Robert N Wine, Zachary T Campbell, Traci M Tanaka Hall#: AM2238, "Peptide, recombinant protein",ImProm-II reverse transcription reaction,Promega,Cat. #: A3803, "Peptide, recombinant protein",GoTaq reaction,Promega,Cat. #: M7123, "Peptide, recombinant protein",T4 polynucleotide kinase

(37) Murine Norovirus Infection Induces T_H1 Inflammatory Responses to Dietary AntigensCell host & microbeNovember 14, 2018Bouziat R, Biering SB, Kouame E, Sangani KA, Kang S, Ernest JD, Varma M, Brown JJ, Urbanek K, Dermody TS, et al.determine MNV genome copies, cDNA was reverse transcribed using IMPROM-II reverse transcriptase (Promega, A3803) with random hexamer according to the manufacturer’s instruction, using 1 μg of total RNA. qPCR was

(38) PyMT-1099, a versatile murine cell model for EMT in breast cancerScientific reportsAugust 14, 2018Saxena M, Kalathur RKR, Neutzner M, Christofori G.Reverse transcription of mRNA was carried out using ImProm-II™ Reverse Transcription System (Promega, A3803) according to the manufacturer’s instructions. mRNA levels were quantified by real-time qPCR using PowerUp SYBR Green Master Mix

(39) In Planta Preliminary Screening of ER Glycoprotein Folding Quality Control (ERQC) ModulatorsInternational Journal of Molecular SciencesJuly 23, 2018Lucia Marti, Andrea Lia, Ida-Barbara Reca, Pietro Roversi, Angelo Santino, Nicole Zitzmann

(40) Viral Replication Complexes Are Targeted by LC3-Guided Interferon-Inducible GTPasesCell host & microbeJuly 12, 2017Biering SB, Choi J, Halstrom RA, Brown HM, Beatty WL, Lee S, McCune BT, Dominici E, Williams LE, Orchard RC, et al.CDNA was reverse transcribed from 1 ug of total RNA using IMPROM-II reverse transcriptase (Promega, A3803) with random hexamer according to the manufacturer’s instruction. qPCR was conducted using SYBR-green

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