GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5004
Quantity500 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) DMAMCL induces ferroptosis in neuroblastoma by targeting HMOX1 in MYCN-amplified subtypes whereas targeting STEAP3 in MYCN-nonamplified subtypesRedox RepDecember 31, 2026Yifan Ma, Simeng Zhang, Dongyang Zhang, Zhongyan Hua, Zhijie Litranscription was performed with 5 μg total RNA using the GoScript™ Reverse Transcription System (catalog no. A5003; Promega, Madison, WI, USA). Quantitative real-time PCR analysis was conducted using TB Green™ Premix

(2) Mitochondria limit coenzyme Q export under cholesterol biosynthetic stressJ Cell BiolAugust 3, 2026Marjana Ndoci, Sharanya Bhattacharya, Ishita Agrawal, Yvonne Hinze, Kathrin Lemke, Anna-Lena Schumacher, Esther Uijttewaal, Ulrich Elling, Steffen Lawo, Patrick Giavalisco, et al.740,955.250). cDNA synthesis was carried out with the Promega GoScript Reverse Transcriptase kit (Cat# A5003) and adjusted to a final concentration of 10 μg/ml. TaqMan Gene Expression Master Mix (Applied Biosystems)

(3) Osteocyte-specific gasdermin D deletion accelerates osteoarthritis via promoting subchondral inflammation and remodelingBone ResJuly 28, 2026Yuki Ogawa, Taku Ebata, Taiki Tokuhiro, Liyile Chen, Ryota Suzuki, Yuki Fujie, Masaya Nakajo, Tsutomu Endo, Hend Alhasan, Masanari Hamasaki, et al.cDNAs using the Go Script reverse transcriptase kit (Promega Corporation, Wisconsin, USA; catalog no. A5003). For extracting RNA from subchondral bone, the knee joints of mice were harvested after 4 weeks of

(4) Epithelial cell expansion drives cyst progression in genetic models of autosomal recessive polycystic kidney diseaseiScienceJune 19, 2026Shuncheng Liu, Xiaole Chen, Zhaoning Liu, Jun Tang, Haoran Tian, Ying Peng, Xiao Mao, Ruping Dai, Bin Zhao, Xugui Li, et al.10606ES60). Complementary DNA (cDNA) was synthesized using GoScript™ Reverse Transcriptase (Promega, A5003). Quantitative PCR (qPCR) was performed with NovoStart®SYBR qPCR SuperMix plus (Novoprotein, E096) and

(5) FERONIA defines intact tissue boundaries through cuticle developmentNature CommunicationsJune 10, 2026Gayeon Kim, Jeongho Choi, Ryeo Jin Kim, Eunkyoo Oh, Seung Yong Shin, Hyun-Soon Kim, Hye Sun Cho, Mi Chung Suh, Hyo-Jun Leewas synthesized with 2 μg RNA as template using GoScriptTM Reverse Transcriptase (Promega, cat. no. A5003) following the manufacturer’s instructions. Quantitative PCR was proceeded with the CFX Opus 96 real-time

(6) Imperfect Maturation of Erythroid Progenitors in Patients with Cirrhosis-Associated AnemiaCurr Issues Mol BiolMay 14, 2026Deepika Lal, Jaswinder Singh Maras, Rakhi Maiwall, Anupam Kumar, Chhagan Biharireverse-transcribed into complementary DNA (cDNA) using the GoScriptTM Reverse Transcription System (A5003; Promega, Madison, WI, USA) according to the manufacturer’s instructions. The cDNA was then amplified

(7) Terahertz Waves Trigger Apoptosis in Cutaneous Squamous Cell Carcinoma via Apoptosis-Inducing Factor Mediated Mitochondrial PathwayCellsApril 29, 2026Liu Sun, Wenxia Wang, Shuocheng She, Lei Wang, Jinwu Zhao, Pandeng Hou, Mingxia Hereverse transcription was performed using the GoScript™ Reverse Transcriptase Kit (Promega, Cat. No: A5003, Madison, WI, USA). QPCR was performed using the GoTaq qPCR Master Mix Kit (Promega, Cat. No: A6001,

(8) MFF budding from mitochondria regulates melanosome size and maturationNature CommunicationsMarch 14, 2026Ana Paula Magalhães Rebelo, Aurora Maracani, Samuele Greco, Federica Dal Bello, Lucia Santorelli, Marco Gerdol, Alberto Pallavicini, Tomas Knedlik, Sara Schiavon, Luca Scorrano, et al.instructions. RNA was reverse transcribed in cDNA using GoScript Reverse Transcriptase (Promega, Cat. No. A5003). For Mff amplification, PCR was performed using melan-a cDNA, PrimeSTAR GXL DNA Polymerase (Takara

(9) A small‑molecule HDAC/PDE modulator activates human adipocyte UCP1 and resolves inflammatory signalingJ Transl MedMarch 5, 2026Mayyasa Rammah, Atilio Reyes Romero, Najeeb Halabi, Sirin Abuaqel, Andrea Guennoun, Alexander Dömling, Jingxuan Shan, Lotfi Chouchanewas reverse transcribed into complementary DNA (cDNA) using GoScript Reverse Transcriptase (Promega, A5003). Quantitative PCR (qPCR) was performed in triplicate on an Applied Biosystems® QuantStudio 6 Flex Real-Time PCR System

(10) Identification and structural characterization of anthrax toxin receptor 2 as the Clostridium perfringens NetF receptorNature CommunicationsFebruary 14, 2026Chang Wang, Filippo Cattalani, Ioan Iacovache, Arunasalam Naguleswaran, Faezeh Farhoosh, Jan Franzen, Laurence Abrami, F. Gisou van der Goot, Horst Posthaus, Benoît Zuberand 1 µg RNA was used for first strand cDNA synthesis using GoScript Reverse Transcriptase (Promega, A5003) with random primers. Real-time PCR was performed using FastStart Universal SYBR Green Master (Roche,

(11) LRRK2R1627P mutation amplifies environmental risk factors induced chronic inflammation and α-synuclein aggregation in the gut of ratsnpj Parkinson's DiseaseFebruary 7, 2026Shimin Pang, Jing Lu, Yanyan Wang, Chao Ying, Chunsong Zhao, Zhenyu Yue, Qiumei Yang, Piu Chantranscription reaction was performed according to the instructions of the Promega reverse transcription kit (A5003). LightCycler® 480 SYBR Green I Master (Roche, 04707516001) to quantify gene expression and GAPDH was

(12) Targeting pre-existing club-like cells in prostate cancer potentiates androgen deprivation therapyEMBO Molecular MedicineFebruary 2, 2026Manon Baurès, Anne-Sophie Vieira Aleixo, Emeline Pacreau, Aysis Koshy, Vanessa Friedrich, Marc Diedisheim, Martin Raigel, Yichao Hua, Charles Dariane, Florence Boutillon, et al.Nucleospin RNA,Macherey-Nagel,740984 SuperScript™ VILO™ cDNA Synthesis Kit,Invitrogen,11754050 GoScript™ Reverse Transcriptase,Promega,A5003 GoTaq(R) qPCR Master Mix,Promega,A6002 In Situ Cell Death Detection Kit (TUNEL),Roche,11 684 809 910 "VECTASTAIN®

(13) Sialic acid–guided spatiotemporal hydrogel therapy for liver cancerMaterials Today BioFebruary 1, 2026Weiqiang Hao, Hyeon Ji Kim, Jumi Kang, Bongkyun Kang, Seoyeon Park, Yuejin Kim, Eunjeong Kim, Kyueui Leeinstructions. cDNA was synthesized from 5 μg of total RNA using the GoScriptTM Reverse Transcriptase kit (A5003; Promega Corporation, Madison, WI, USA). qRT-PCR was performed using SYBR® Green Realtime PCR Master

(14) Construction and Immunogenicity Evaluation of a Recombinant Infectious Bronchitis Virus H120-Based Vaccine in Broiler ChickensAnimals (Basel)January 22, 2026Ali Nayef, Sara Jibreen, Mustafa AbabnehComplementary DNA (cDNA) was synthesized using the GoScript™ Reverse Transcription System (Promega, Cat. No. A5003) (Promega, Madison, WI, USA). For each reaction, two separate mixtures were prepared as follows: Solution

(15) Neutrophil depletion at the early stage of Japanese encephalitis virus infection affects CD8+ T cell infiltration into the mouse brain and causes severe encephalitisFrontiers in ImmunologyJanuary 21, 2026Rohit Soni, Prasanjit Jena, B. Kusuma, Arup Banerjee(15-596-018; Invitrogen, Carlsbad, CA, USA). In two steps, a GoScriptTM Reverse Transcription System from (A5003; Promega, Madison, WI, USA) was used to create cDNA. Gene expression was measured by qRT-PCR in triplicate

(16) Proline transporters balance the salicylic acid-mediated trade-off between regeneration and immunity in plantsbioRxivJanuary 16, 2026Dawei Xu, Zeinu Mussa Belew, Nathália Cássia Ferreira Dias, Li Wang, Andy Zhang, Yun-Fan Stephanie Chen, Carter James Newton, Feng Kong, Yaochao Zheng, Yao Yao, et al.Omega BIO-TEK). First-strand cDNA was synthesized from total RNA using GoScript™ Reverse Transcriptase (A5003, Promega). Real-time RT-PCR was conducted with iQ SYBR Green Supermix (Bio-rad) on the Applied Biosystems QuantStudio 5 real-time PCR system

(17) Glis3 as a critical regulator of Pit1-lineages and renal functionsJ Mol Med (Berl)December 29, 2025Giuditta Rurale, Ilaria Gentile, Luca Persani, Federica MarelliComplementary DNA (cDNA) synthesis was performed using GoScript Reverse Transcription System (Promega, A5003). Quantitative real-time PCR (qRT-PCR) was conducted on an ABI PRISM 7900HT Fast Real-Time PCR System

(18) Unveiling the conserved mechanism of microsporidian vertical transmission: A comparative study of nosema infection across Host speciesVirulenceDecember 24, 2025Chunxia Wang, Yongzhi Kong, Zishen Tang, Tongyu Luo, Xuanang Yang, Yongjun Zhang, Tian Li, Zeyang ZhouThe cDNA was synthesized with 1 μg total RNA using the GoScript™ Reverse Transcription System Kit (A5003, Promega) after DNA digestion with DNase I. Relative ApVg mRNA levels were measured using qPCR with

(19) Regulation of vacuole fusion in stomata by dephosphorylation of the HOPS subunit VPS39Plant JDecember 19, 2025Anne-Marie Pullen, Grant Billings, Charles Hodgens, Gisele White, Belinda S. Akpa, Marcela Rojas-PierceReverse transcription was conducted using GoScript Reverse Transcriptase (Promega, Madison, WI, USA; A5003). qPCR was conducted using the Bio‐Rad CFX Connect real‐time PCR system and SYBR Green Master mix (Bio‐Rad,

(20) B-Cell Receptor-Associated Protein 31 Deficiency Aggravates Ethanol-Induced Liver Steatosis and Liver Injury via Attenuating Fatty Acid Oxidation and Glycogen SynthesisInt J Mol SciDecember 18, 2025Shubin Yu, Yaodong Xia, Chunyan Zhang, Xiangyue Han, Xiaoyue Feng, Liya Li, Hang Ma, Jialin XuSpectrophotometers and gel electrophoresis. Two micrograms of total RNA were converted to single-stranded cDNA (A5003, Promega Corporation; Madison, WI, USA). The relative mRNA levels were quantified using a CFX96 Touch™ Real-Time PCR Detection System

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