GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5003
Quantity100 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) MFF budding from mitochondria regulates melanosome size and maturationNature CommunicationsMarch 14, 2026Ana Paula Magalhães Rebelo, Aurora Maracani, Samuele Greco, Federica Dal Bello, Lucia Santorelli, Marco Gerdol, Alberto Pallavicini, Tomas Knedlik, Sara Schiavon, Luca Scorrano, et al.instructions. RNA was reverse transcribed in cDNA using GoScript Reverse Transcriptase (Promega, Cat. No. A5003). For Mff amplification, PCR was performed using melan-a cDNA, PrimeSTAR GXL DNA Polymerase (Takara

(2) A small‑molecule HDAC/PDE modulator activates human adipocyte UCP1 and resolves inflammatory signalingJ Transl MedMarch 5, 2026Mayyasa Rammah, Atilio Reyes Romero, Najeeb Halabi, Sirin Abuaqel, Andrea Guennoun, Alexander Dömling, Jingxuan Shan, Lotfi Chouchanewas reverse transcribed into complementary DNA (cDNA) using GoScript Reverse Transcriptase (Promega, A5003). Quantitative PCR (qPCR) was performed in triplicate on an Applied Biosystems® QuantStudio 6 Flex Real-Time PCR System

(3) Identification and structural characterization of anthrax toxin receptor 2 as the Clostridium perfringens NetF receptorNature CommunicationsFebruary 14, 2026Chang Wang, Filippo Cattalani, Ioan Iacovache, Arunasalam Naguleswaran, Faezeh Farhoosh, Jan Franzen, Laurence Abrami, F. Gisou van der Goot, Horst Posthaus, Benoît Zuberand 1 µg RNA was used for first strand cDNA synthesis using GoScript Reverse Transcriptase (Promega, A5003) with random primers. Real-time PCR was performed using FastStart Universal SYBR Green Master (Roche,

(4) LRRK2R1627P mutation amplifies environmental risk factors induced chronic inflammation and α-synuclein aggregation in the gut of ratsnpj Parkinson's DiseaseFebruary 7, 2026Shimin Pang, Jing Lu, Yanyan Wang, Chao Ying, Chunsong Zhao, Zhenyu Yue, Qiumei Yang, Piu Chantranscription reaction was performed according to the instructions of the Promega reverse transcription kit (A5003). LightCycler® 480 SYBR Green I Master (Roche, 04707516001) to quantify gene expression and GAPDH was

(5) Targeting pre-existing club-like cells in prostate cancer potentiates androgen deprivation therapyEMBO Molecular MedicineFebruary 2, 2026Manon Baurès, Anne-Sophie Vieira Aleixo, Emeline Pacreau, Aysis Koshy, Vanessa Friedrich, Marc Diedisheim, Martin Raigel, Yichao Hua, Charles Dariane, Florence Boutillon, et al.Nucleospin RNA,Macherey-Nagel,740984 SuperScript™ VILO™ cDNA Synthesis Kit,Invitrogen,11754050 GoScript™ Reverse Transcriptase,Promega,A5003 GoTaq(R) qPCR Master Mix,Promega,A6002 In Situ Cell Death Detection Kit (TUNEL),Roche,11 684 809 910 "VECTASTAIN®

(6) Sialic acid–guided spatiotemporal hydrogel therapy for liver cancerMaterials Today BioFebruary 1, 2026Weiqiang Hao, Hyeon Ji Kim, Jumi Kang, Bongkyun Kang, Seoyeon Park, Yuejin Kim, Eunjeong Kim, Kyueui Leeinstructions. cDNA was synthesized from 5 μg of total RNA using the GoScriptTM Reverse Transcriptase kit (A5003; Promega Corporation, Madison, WI, USA). qRT-PCR was performed using SYBR® Green Realtime PCR Master

(7) Construction and Immunogenicity Evaluation of a Recombinant Infectious Bronchitis Virus H120-Based Vaccine in Broiler ChickensAnimals (Basel)January 22, 2026Ali Nayef, Sara Jibreen, Mustafa AbabnehComplementary DNA (cDNA) was synthesized using the GoScript™ Reverse Transcription System (Promega, Cat. No. A5003) (Promega, Madison, WI, USA). For each reaction, two separate mixtures were prepared as follows: Solution

(8) Neutrophil depletion at the early stage of Japanese encephalitis virus infection affects CD8+ T cell infiltration into the mouse brain and causes severe encephalitisFrontiers in ImmunologyJanuary 21, 2026Rohit Soni, Prasanjit Jena, B. Kusuma, Arup Banerjee(15-596-018; Invitrogen, Carlsbad, CA, USA). In two steps, a GoScriptTM Reverse Transcription System from (A5003; Promega, Madison, WI, USA) was used to create cDNA. Gene expression was measured by qRT-PCR in triplicate

(9) Glis3 as a critical regulator of Pit1-lineages and renal functionsJ Mol Med (Berl)December 29, 2025Giuditta Rurale, Ilaria Gentile, Luca Persani, Federica MarelliComplementary DNA (cDNA) synthesis was performed using GoScript Reverse Transcription System (Promega, A5003). Quantitative real-time PCR (qRT-PCR) was conducted on an ABI PRISM 7900HT Fast Real-Time PCR System

(10) Unveiling the conserved mechanism of microsporidian vertical transmission: A comparative study of nosema infection across Host speciesVirulenceDecember 24, 2025Chunxia Wang, Yongzhi Kong, Zishen Tang, Tongyu Luo, Xuanang Yang, Yongjun Zhang, Tian Li, Zeyang ZhouThe cDNA was synthesized with 1 μg total RNA using the GoScript™ Reverse Transcription System Kit (A5003, Promega) after DNA digestion with DNase I. Relative ApVg mRNA levels were measured using qPCR with

(11) Regulation of vacuole fusion in stomata by dephosphorylation of the HOPS subunit VPS39Plant JDecember 19, 2025Anne-Marie Pullen, Grant Billings, Charles Hodgens, Gisele White, Belinda S. Akpa, Marcela Rojas-PierceReverse transcription was conducted using GoScript Reverse Transcriptase (Promega, Madison, WI, USA; A5003). qPCR was conducted using the Bio‐Rad CFX Connect real‐time PCR system and SYBR Green Master mix (Bio‐Rad,

(12) B-Cell Receptor-Associated Protein 31 Deficiency Aggravates Ethanol-Induced Liver Steatosis and Liver Injury via Attenuating Fatty Acid Oxidation and Glycogen SynthesisInt J Mol SciDecember 18, 2025Shubin Yu, Yaodong Xia, Chunyan Zhang, Xiangyue Han, Xiaoyue Feng, Liya Li, Hang Ma, Jialin XuSpectrophotometers and gel electrophoresis. Two micrograms of total RNA were converted to single-stranded cDNA (A5003, Promega Corporation; Madison, WI, USA). The relative mRNA levels were quantified using a CFX96 Touch™ Real-Time PCR Detection System

(13) Anti‐melanogenic effects of Medicago sativa seed extracts on melanocytesInternational Journal of Cosmetic ScienceDecember 1, 2025Chae Rin Kim, Jung Woo, Kyu Lim Kim, Minah Choi, Hee Jung Shin, Junoh Kim, Kyung Min Lim, Chang‐Seok Leeinstructions. cDNA was synthesized from total mRNA using the GoScript Reverse Transcription System (A5003; Promega, Madison, WI, USA) and a thermal cycler (BioRad, Hercules, CA, USA). Tyrosinase (qMmuCED0045245,

(14) Olfactory gene dynamics in invasive Indian and non-invasive African malaria vectors at the crossroads of development, infection and resistanceSci RepOctober 28, 2025Arvind Sharma, Bhuvan Dixit, Bharti Goyal, Renuka Harit, Jatin Kumar, Shibani Biswas, Ritu Goswami, Kailash C. Pandey, S. Noushin Emami, Soumyananda Chakraborticharacterized using quantitative RT-PCR (qRT-PCR). GoScript™ Reverse Transcriptase cDNA synthesis kit (Promega, A5003) was used to convert 1ug of total RNA to cDNA following manufacturer protocol. Briefly, cDNA from different

(15) COG6 is an essential host factor for influenza A virus infectionMicrobiology SpectrumOctober 7, 2025Daobin Feng, Jiaohan Guo, Jingjing Yan, Jian Chen, Longfei Ding, Xinyu Zhu, Zhilu Chen, Yangyang Hu, Miaomiao Zhang, Jian Liu, et al.synthesized from total RNA isolated from WT A549 cells using GoScript Reverse Transcriptase (Promega, A5003). The coding sequence of COG6, fused with either a Flag or Myc tag, was cloned into pCAGGS vector for

(16) Biofloc system with synbiotic supplementation improves Penaeus vannamei immunity against AHPNDAppl Microbiol BiotechnolOctober 3, 2025Magdalena Lenny Situmorang, Anggie Nur Fadillah, Atian Azizy, Gede Suantikavisualization (Supplementary File). Next, the total RNA was converted into cDNA using the GoScript™ RT System (A5003, Promega) according to the manufacturer’s instructions. The cDNA samples obtained were then used as

(17) Glycans on non-structural protein 1 prevent premature T-cell mediated dengue virus clearanceEMBO Molecular MedicineSeptember 17, 2025Fakhriedzwan Idris, Justin Seng Geap Ooi, Donald Heng Rong Ting, Eunice Tze Xin Tan, Corrine Wan, Peter I Benke, Jan K Marzinek, Jack M Copping, Qin Hui Li, Lu Yi Ng, et al.kits,Molecular biology kits QIAamp Viral RNA,Qiagen,52904 GoScript™ Reverse Transcriptase,Promega,A5003 NEB Q5 Hot-Start high-fidelity 2× Master Mix,New England Biolabs,M0494S pCR™-Blunt II-TOPO™ Vector,Invitrogen,K280002 MinElute

(18) Establishing a pediatric solid tumor PDX biobank for precision oncology researchCancer Biol TherAugust 13, 2025Larissa Akemi Kido, Milena Rodrigues Marusco, Ellen Aparecida da Silva, Laís Do Carmo, Ana Beatriz Teodoro Borges, Felipe Luz Torres Silva, Juliana Silveira Ruas, Dieila Giomo de Lima, Larissa de Abreu Fernandes, Camila Maia Martin Daiggi, et al.synthesized following the GoScript Reverse Transcription System protocol (Promega™, Madison, WI, USA, Cat#A5003). The quality of synthesized cDNA was verified through control PCR for commonly expressed housekeeping

(19) Filamented Light (FLight) Bioprinting of Mini‐Muscles with Self‐Renewal PotentialAdvanced MaterialsJuly 23, 2025Hao Liu, Michael Winkelbauer, Jakub Janiak, Patrick Weber, Ali Kerem Kalkan, Inseon Kim, Parth Chansoria, Ori Bar‐Nur, Marcy Zenobi‐Wongtranscribed to complementary DNA following the instruction of GoScript Reverse Transcriptase kit (Promega, A5003). The relative gene expression levels were determined on real‐time PCR System (Applied Biosystems, QuantStudio

(20) High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminaseNature BiotechnologyJuly 7, 2025Yuan Wu, Yu-Lan Xiao, Weixin Tangcooled down on ice. Following primer annealing, 0.5 μL of the GoScript reverse transcriptase (Promega, A5003) was added together with 2 μL of 5× GoScript RT buffer, 1 μL of 25 mM MgCl2, 0.5 μL of 10 mM dNTPs,

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