M-MLV Reverse Transcriptase Buffer Pack from Promega

Product Specs
ItemM-MLV Reverse Transcriptase Buffer Pack
CompanyPromega
Price $15.00Supplier Page View Company Product Page
Catalog NumberM5313
Quantity2 x 1ml
TypeBuffer

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Targeted long-read RNA sequencing for rare disease diagnosis and variant interpretationSci AdvApril 17, 2026Robert Wang, Feng Wang, Nicole DeBruyne, Xinjun Ji, Nicole M. Engelhardt, Joseph Jee-Hwan Park, Amber Notaro, Samantha Gaerlan, Ryan Park, Matthew J. Schultz, et al.(M-MLV) Reverse Transcriptase (Promega, #M1701), and 1× M-MLV Reverse Transcriptase Buffer (Promega, #M5313) according to the manufacturer’s instructions for 1 hour at 37°C. RT products were then PCR amplified

(2) Metastatic breast cancer cells are vulnerable to fatty acid oxidation inhibition through DDX3-DRP1-mediated mitochondrial plasticityRedox BiologyOctober 1, 2025Wen-Jing Hsu, Ming-Chien Hsu, Cheng-Ying Chu, Yu-Cheng Lee, Ching-Chieh Yang, Zei-Wei Liu, Chi-Ching Lee, Yang-Sen Lin, Cheng-Wei LinGENzolTM TriRNA Pure kit (GZX100, Geneaid). cDNA was synthesized with M-MLV reverse transcriptase (M5313, Promega) according to the manufacturer's instructions. cDNA was analyzed by a qPCR with GoTaq qPCR Master Mix

(3) Andrographolide Mitigates Inflammation and Reverses UVB-Induced Metabolic Reprogramming in HaCaT CellsInt J Mol SciJuly 6, 2025Carolina Manosalva, Pablo Alarcón, Lucas Grassau, Carmen Cortés, Juan L. Hancke, Rafael A. BurgosFor cDNA production, 200 ng of RNA underwent reverse transcription using M-MLV reverse transcriptase (M5313, Promega, Madison, WI, USA) as per the manufacturer’s instructions. RT-qPCR was conducted using Takyon

(4) Hydrogen sulfide alleviates high-salt-stimulated myocardial fibrosis through inhibiting hypoxia-inducible factor-1αFrontiers in PharmacologyJune 26, 2025Qian Peng, Pan Huang, Boyang Lv, Chaoshu Tang, Hongfang Jin, Yaqian HuangDNA was generated using oligo (dT) primers (Cat# C110A; Promega, United States) and an M-MLV enzyme (M5313; Promega, United States). qPCR assays were then conducted using the GoTaq Master Mix (Promega, A600A,

(5) Effects of heterozygous SMG1 mutations on nonsense-mediated mRNA decay in human pluripotent stem cell modelMolecules and CellsMay 20, 2025Chanyoung Lee, Jin Sook Lee, Yejin Kwon, Aeri Shin, Tae Yeong Jeong, Jiyun Yang, Jung Woo Hwang, Hyeong-In Kim, Hee-Jung Choi, Yoon Ki Kim, et al.reverse- transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega, M5313). The resulting cDNA was amplified with gene-specific primers. Quantitative PCR (qPCR) analysis was

(6) Group A Streptococcal asparagine metabolism regulates bacterial virulenceEMBO ReportsApril 14, 2025Abhinay Sharma, Aparna Anand, Miriam Ravins, Xiaolan Zhang, Nicola Horstmann, Samuel A Shelburne, Kevin S McIver, Emanuel HanskiRQ1 RNase-Free DNase,Promega corporation,Cat# M6101 M-MLV Reverse Transcriptase,Promega corporation,Cat# M5313 2x Tamix Fast SyGreen Mix Hi-ROX,Tamar Laboratory Supplies Ltd.,Cat# TA20.12-05 Pan-Bacteria (RNA-Seq)

(7) Different fibroblast subtypes propel spatially defined ileal inflammation through TNFR1 signalling in murine ileitisNature communicationsMarch 28, 2025Iliopoulou L, Tzaferis C, Prados A, Roumelioti F, Koliaraki V, Kollias G.cDNA was synthesized using the Moloney Murine Leukemia Virus Reverse Transcriptase, (M-MLV, Promega, M5313). Real-time polymerase chain reaction was performed in the CFX96 Real-Time System (Bio-Rad) according

(8) Ovarian steroids modulate mRNA expression of ECM associated genes and collagen deposition induced by TGF β1 in equine endometrium in vitroSci RepJanuary 2, 2025Amaral Ana, Sadowska Agnieszka, Cerveira-Pinto Marta, Kordowitzki Pawel, Skarzynski Dariusz, Ferreira-Dias Graça, Anna Szóstek-Mioduchowskaelectrophoresis. The reverse transcription to obtain cDNA was made using M-MLV reverse transcriptase enzyme (M5313; Promega; Madison, USA) from 1000 ng total RNA in a 20 µL reaction volume using oligo(dT) primer (C1101;

(9) Exclusive enteral nutrition initiates individual protective microbiome changes to induce remission in pediatric Crohn’s diseaseCell Host & MicrobeNovember 13, 2024Deborah Häcker, Kolja Siebert, Byron J. Smith, Nikolai Köhler, Alessandra Riva, Aritra Mahapatra, Helena Heimes, Jiatong Nie, Amira Metwaly, Hannes Hölz, et al.Kit,Macherey-Nagel,Cat#740955.250 NucleoSpin® gDNA columns,Macherey-Nagel,Cat#740230 M-MLV Reverse Transcriptase,Promega,Cat#M1705; Cat#M5313 QIAamp DNA Mini Kit,Qiagen,Cat#51306 Deposited data,Deposited data,Deposited data "untargeted metabolomics

(10) Linoleic Acid Induces Metabolic Reprogramming and Inhibits Oxidative and Inflammatory Effects in Keratinocytes Exposed to UVB RadiationInternational Journal of Molecular SciencesSeptember 26, 2024Carolina Manosalva, Claudio Bahamonde, Franco Soto, Vicente Leal, César Ojeda, Carmen Cortés, Pablo Alarcón, Rafael A. BurgosSubsequently, 200 ng of RNA was reverse-transcribed for cDNA synthesis utilizing M-MLV reverse transcriptase (#M5313, Promega, Madison, WI, USA), following the manufacturer’s instructions. RT-qPCR assays were conducted

(11) FOXO1 regulates expression of Neurod4 in the pituitary glandMolecular and Cellular EndocrinologyApril 1, 2024Caitlin E. Stallings, Pratyusa Das, Sandria W. Athul, Arnold E. Ukagwu, Philip J. Jensik, Buffy S. Ellsworthμg) was reversed transcribed to cDNA using the Promega M-MLV kit according to included instructions (M5313, Promega, Madison, WI, USA). Ten ng of cDNA was used for mRNA analysis. All samples were run in duplicate

(12) Cisplatin-induced activation of TGF-β signaling contributes to drug resistanceOncology ResearchJanuary 1, 2024SAYAKA IMATSUJI, YUKIKO UJIE, HIROYUKI ODAKE, MASAYA IMOTO, SUSUMU ITOH, ETSU TASHIROμg) was reverse transcribed into cDNA using MM-LV reverse transcriptase (Promega, Madison, WI, USA, #M5313) according to the manufacturer’s instructions. Quantitative PCR was performed on a Thermal Cycler Dice

(13) Neutralizing antibodies from prior exposure to dengue virus negatively correlate with viremia on re-infectionCommunications MedicineOctober 19, 2023Anbalagan Anantharaj, Tanvi Agrawal, Pooja Kumari Shashi, Alok Tripathi, Parveen Kumar, Imran Khan, Madhu Pareek, Balwant Singh, Chitra Pattabiraman, Saurabh Kumar, et al.reaction mixture contains 11.3 μl of nuclease free water, 5 μl of M-MLV RT 5X buffer (Promega, cat no: M5313), 1.5 µl of 25 mM MgCl2 (NEB, cat no: B9021S), 0.5 μl of 10 mM dNTPs (Promega, cat no: U1330), 1.25

(14) Inhibition of Myeloperoxidase Pro-Fibrotic Effect by Noscapine in Equine EndometriumInternational Journal of Molecular SciencesFebruary 10, 2023Ana Amaral, Nélio Cebola, Anna Szóstek-Mioduchowska, Maria Rosa Rebordão, Paweł Kordowitzki, Dariusz Skarzynski, Graça Ferreira-DiasHayward, CA, USA). Therefore, cDNA synthesis was achieved using the M-MLV reverse transcriptase enzyme (M5313; Promega, Madison, WI, USA) from 1 µg of total RNA in a 20 µL reaction volume, using an oligo (dT) primer

(15) Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cellsMolecular BrainDecember 1, 2022Ju-Hyun Lee, Geon Yoo, Juhyun Choi, Si-Hyung Park, Hyogeun Shin, Renuka Prasad, Yeunehee Lee, Mee Ryung Ahn, Il-Joo Cho, Woong Sunμg) was used to synthesize cDNA using murine Moloney leukemia virus reverse transcriptase (Promega, M5313). cDNA was then amplified using gene-specific primers (primer sequences are listed in Additional file

(16) Depletion of cardiac cardiolipin synthase alters systolic and diastolic functioniScienceNovember 19, 2021Elia Smeir et al.,Worthington Biochemical Corporation,LS004174 Laminin,, PBS,Gibco,14190-094 Ethanol,Merck,CAS 64-17-5 Claycomb medium,Sigma-Aldrich,# 51800C dNTPs,Promega,U1511 M-MLV RT 5x Buffer,Promega,M5313 Random primer,Promega,C1181 Power SYBR green PCR mix,Thermofisher,4367659 XF base media,Agilent Technologies,#

(17) The Inhibitory Effect of Noscapine on the In Vitro Cathepsin G-Induced Collagen Expression in Equine EndometriumLife (Basel)October 19, 2021Ana Amaral, Carina Fernandes, Anna Szóstek-Mioduchowska, Karolina Lukasik, Maria Rosa Rebordão, Pedro Pinto-Bravo, Dariusz Jan Skarzynski, Graça Ferreira-DiasBiotium, Hayward, CA, USA). The synthesis of cDNA was performed using M-MLV reverse transcriptase enzyme (M5313; Promega, Madison, WI, USA) from 1 μg of total RNA in a 20 μL reaction volume using oligo (dT) primer

(18) Noscapine Acts as a Protease Inhibitor of In Vitro Elastase-Induced Collagen Deposition in Equine EndometriumInternational Journal of Molecular SciencesMay 19, 2021Ana Amaral, Carina Fernandes, Anna Szóstek-Mioduchowska, Maria Rosa Rebordão, Dariusz Jan Skarzynski, Graça Ferreira-Diasof 28S and 18S rRNA bands. The synthesis of cDNA was undertaken using reverse transcriptase enzyme (M5313; Promega; Madison, WI, USA) and oligo (dT) primer (C1101; Promega; Madison, WI, USA) from 1000 ng total

(19) Change in Sucrose Cleavage Pattern and Rapid Starch Accumulation Govern Lily Shoot-to-Bulblet Transition in vitroFrontiers in Plant ScienceJanuary 14, 2021Yun Wu et al.reverse-transcribed using M-MLV reverse transcriptase according to the manufacturer’s instruction (M5313, Promega, Madison, WI, United States). qRT-PCR was used to detect the relative expression levels of

(20) Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1Cellular and molecular gastroenterology and hepatologyJanuary 1, 2021Dunbar K, Valanciute A, Lima ACS, Vinuela PF, Jamieson T, Rajasekaran V, Blackmur J, Ochocka-Fox AM, Guazzelli A, Cammareri P, et al.using M-MLV reverse transcriptase (Promega, M1701) and M-MLV reverse transcriptase buffer (Promega, M5313) following manufacturer’s instructions. Quantitative real-time polymerase chain reaction was completed

Be the First to Write a Review!