SNAP-Surface Block from New England Biolabs

Description

SNAP-Surface Block™ (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity of the SNAP-tag™ on the surface of live cells. It can be used to generate inactive controls in live cell labeling experiments performed with SNAP-tag fusion proteins. SNAP-Surface Block reacts with the SNAP-tag irreversibly, inactivating it for subsequent labeling steps. This blocker is largely membrane impermeable essentially limiting blocking to cell surface-exposed SNAP-tags

Product Specs
ItemSNAP-Surface Block
CompanyNew England Biolabs
Price $158.00 Inquire
Catalog NumberS9143S
ApplicationsCellular Biology
Temperature-20 C
Quantity200 nmol

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Lysosomal down-regulation of the mu opioid receptor is opposed by the Retromer complexSci AdvMarch 20, 2026Aleksandra Dagunts, Hayden Adoff, Brandon Novy, Lamya Ben Ameur, Monica De Maria, Arpiar Saunders, Braden T. Lobingierresuspended at 100 μM in anhydrous DMSO, and stored as frozen aliquots at −20°C. SNAP-Surface Block (S9143S) was purchased from New England Biolabs (NEB), resuspended at 1 mM in anhydrous DMSO, and stored as

(2) Posttranslational microtubule modification alters podocalyxin-trafficking in epithelial cellsFrontiers in Cell and Developmental BiologySeptember 22, 2025Lena-Sophie Gorek, Dominik Trauth, Natalia Kamm, Lana Sophie Schiffke, Carlotta Zerbian, Lina-Marie Mende, Ralf JacobStates)" SNAP-Surface Alexa Fluor 647 (S9136S),"New England Biolabs (Ipswich, United States)" SNAP-Surface Block (S9143S),"New England Biolabs (Ipswich, United States)" secondary antibodies/dyes used in this study.

(3) High hedgehog signaling is transduced by a multikinase-dependent switch controlling the apico-basal distribution of the GPCR smoothenedeLifeSeptember 9, 2022Marina GonÃ§alves Antunes, Matthieu Sanial, Vincent Contremoulins, Sandra Carvalho, Anne Plessis, Isabelle Becam,New England Biolabs,Cat#S9132S,3.3 µM "Chemical compound, drug",SNAP-Surface Block,New England Biolabs,Cat#S9143S,2 µM "Chemical compound, drug",SNAP-Surface Alexa Fluor 647,New England Biolabs,Cat#S9136S,3.3 µM "Software,

(4) Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretionMolecular metabolismSeptember 1, 2022Yang L, Fye MA, Yang B, Tang Z, Zhang Y, Haigh S, Covington BA, Bracey K, Taraska JW, Kaverina I, et al.5′-TAACAACTGTGGTCGCTGCGAAGTATGAGGGA-3’. SNAP substrates were purchased from NEB (SNAP-Surface® Block, S9143S; SNAP-Surface® Alexa Fluor® 546, S9132S; SNAP-Surface® Alexa Fluor® 647, S9136S). 2.2 Lentivirus production HEK293FT

(5) Site‐Specific Protein Labeling with SNAP‐TagsCurrent Protocols in Protein ScienceSeptember 24, 2013Nelson B. Coleis to be labeled (i.e. via exocytosis), a cell impermeable blocking agent (SNAP-Surface Block, NEB #S9143S) should be used. Place dish at 4°C for 15 min. For the “pulse” rinse the wells for the 0 time point

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