Luciferase Assay Reagent from Promega

Product Specs
ItemLuciferase Assay Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1483
Quantity100ml

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Crystal structure of the Legionella pneumophila effector SidL (Lpg0437) in complex with its metaeffector LegA11 (Lpg0436)VirulenceMarch 17, 2026Dominik A. Machtens, Carissa A. Hutchison, Ashley M. Stein, Jan Eberhage, Jonas M. Willerding, Susanne Eschenburg, Stephanie R. Shames, Thomas F. Reuboldwere run in triplicate for 90 min at 30°C followed by addition of Luciferase assay reagent (Promega, E1483) and luminescence (arbitrary units) was quantified using a BioTek Synergy HTX multimode plate reader

(2) The serotonin receptor 2b (5-HT2B) modulates heart remodeling following myocardial infarction via regulation of Hippo pathwayiScienceFebruary 20, 2026Ryan Potter, Min Zi, Sukhpal Prehar, Tara McNulty, Dowan Kwon, Ellie England, Maram Almutairi, Nicholas Stafford, Ardiansah Bayu Nugroho, Efta Triastuti, et al.assays,Critical commercial assays,Critical commercial assays ,, luciferase assay reagent,Promega,Cat. #E1483 Click-iT EdU Cell Proliferation Kit for Imaging,Thermo Fisher Scientific,Cat. #C10337 In Situ Cell

(3) Strong and early immune responses against SARS-CoV-2 in mice and rhesus macaques after BNT162b3 vaccinationnpj VaccinesJanuary 13, 2026Annette B. Vogel, Bonny G. Lui, Kristin Tompkins, Isis Kanevsky, Alexander Muik, Stefanie A. Krumm, Alptekin Güler, Mohan S. Maddur, Kerstin C. Walzer, Sonja Witzel, et al.h. Supernatants were removed, and the cells were lysed with luciferase assay reagent (Promega, cat# E1483). Luminescence was recorded on a CLARIOstar® Plus microplate reader (BMG Labtech), and neutralization

(4) Gut microbiota promotes immune tolerance at the maternal-fetal interfaceCellJanuary 8, 2026Julia A. Brown, Mohammed Amir, Shui Yu, Daniel S.H. Wong, Jinghua Gu, Uthra Balaji, Christopher N. Parkhurst, Seunghee Hong, Lucy R. Hart, Hannah C. Carrow, et al.in cell culture and measuring luciferase expression using the Promega Luciferase Assay Reagent (Promega E1483). To measure abundance of AhR-activating ligands, transduced cells were cultured in the presence of

(5) Complete neutralizing antibody evasion by serodivergent non-mammalian AAVs enables gene therapy redosingCell Reports MedicineDecember 16, 2025Ezra J. Loeb, Sophia A. Fergione, Vivian Yudistyra, Marco M. Fanous, Abigail R. Benkert, Delaney G. Fisher, Joshua A. Hull, Mai K. ElMallah, Aravind AsokanAssays,Critical Commercial Assays ,, Passive Lysis 5X Buffer,Promega,E1941 Luciferase Assay Reagent,Promega,E1483 TRIzol Reagent,Thermo Fisher,15596026 High-Capacity cDNA Reverse Transcription Kit,Thermo Fisher,4368814 TURBO

(6) DNA methylation of the endothelin receptor B makes blue fish yellowNature CommunicationsDecember 7, 2025Wei Fang, Danielle Blakkan, Alexandra Byrne, Gail Lee, Roya Bashier, Russell D. Fernald, Sebastian G. Alvaradoextracts using a 1X luciferase lysis solution (E1531, Promega) and adding to 100ul of luciferase reagent (E1483, Promega) followed by measurements in 96 well format on a Cytation 3 multi-mode plate reader (Biotek)

(7) Buffer Valency Engineering Enables High-concentration and Shelf-stable DNA Transfection Particles for Viral Vector ProductionResearch SquareOctober 23, 2025Hai-Quan Mao, Jinghan Lin, Turash H. Pial, Kailei D. Goodier, Kailei Ding, Marine Guise, Marine Brailsford, Paetra Brailsford, Sixuan Choi-Ali, Sixuan Zhu, et al.For luciferase measurements, 20 μL of each sample was mixed with 100 μL luciferase substrate (Cat. #E1483, Promega Co.) and the luminescence signal was measured by a luminometer. For tdTomato fluorescence assessment,

(8) A dual-mechanism antimicrobial peptide with antimutagenic activity targets the replisome and induces cell envelope stressmSphereSeptember 30, 2025Amanda Holstad Singleton, Olaug Elisabeth Torheim Bergum, Jana Scheffold, Synnøve Brandt Ræder, Lilja Brekke Thorfinnsdottir, Lisa Marie Røst, Caroline Krogh Søgaard, Per Bruheim, Marit Otterleih. After stopping the reaction for 5 min in an ice bath, 25 µL of Luciferase Assay Reagent (Promega, E1483) was added to each reaction. Twenty microliters of each reaction was then added to 50 µL Luciferase Assay Reagent

(9) Metformin attenuates alveolar bone destruction in mice with apical periodontitis and inhibits pro-inflammatory cytokine synthesis in lipopolysaccharide-stimulated RAW264.7 through the AMPK-mTOR-NF-κB pathwayFrontiers in ImmunologyJuly 31, 2025Chunmei Ren, Kento Tazawa, Nobuyuki Kawashima, Risa Ohshima, Yamato Okada, Shihan Wang, Ziniu Yu, Peifeng Han, Yujin Ohsugi, Sayaka Katagiri, et al.from Thermo Fisher Scientific. The cells were lysed using a luciferase cell culture lysis reagent (#E1483, Promega). Subsequently, the activity of the light-producing enzyme luciferase was measured using a

(10) A transcription factor ensemble orchestrates bundle sheath expression in riceNature CommunicationsJuly 31, 2025Lei Hua, Na Wang, Susan Stanley, Ruth M. Donald, Satish Kumar Eeda, Kumari Billakurthi, Ana Rita Borba, Julian M. Hibberdabove, LUC activity was measured with 20 μl of protein sample and 100 μl of LUC assay reagent (Promega, E1483) using Clariostar plate reader. Transcriptional activity from the promoter was calculated as LUC luminescence

(11) Ginsenoside Rb2 demonstrates potent antiviral activity against Zika virus infectionJournal of Ginseng ResearchJuly 1, 2025Tong Wu, Ting Wang, Hanlin Chen, Zhongyuan Tan, Yuanjiu Miao, Ning Chen, Zhenhua Zhengconcentrations of G-Rb2, and cultures were maintained for 12, 24 or 48 h. Luciferase Assay Reagent (E1483, Promega, Madison, WI, USA) was used to measure luciferase activity after transfection with subsequent

(12) Neuroactive steroids activate membrane progesterone receptors to induce sex specific effects on protein kinase activityiScienceMay 16, 2025Abigail H.S. Lemons, Briana Murphy, Jake S. Dengler, Seda Salar, Paul A. Davies, Joshua L. Smalley, Stephen J. Mossassays,Critical commercial assays,Critical commercial assays ,, Luciferase Assay Reagent,Promega,E1483 Luciferase Cell Culture Lysis Reagent,Promega,E1500 Mycoplasma Detection Kit,Assay Genie,M0RV001 ,, Experimental models

(13) Expression of Prooncogenic Nuclear Receptor 4A (NR4A)-Regulated Genes β1-Integrin and G9a Inhibited by Dual NR4A1/2 LigandsInt J Mol SciApril 21, 2025Lei Zhang, Victoria Gatlin, Shreyan Gupta, Michael L. Salinas, Selim Romero, James J. Cai, Robert S. Chapkin, Stephen Safelysed using a freeze–thaw protocol, and 30 μL of cell extract was used for determining luciferase (E1483, Promega, Madison, WI, USA) and β-gal (T1007, Invitrogen, Waltham, MA, USA) activities. LumiCount (Packard,

(14) TGM6 is a helminth secretory product that mimics TGF-β binding to TGFBR2 to antagonize signaling in fibroblastsNat CommunFebruary 21, 2025Stephen E. White, Tristin A. Schwartze, Ananya Mukundan, Christina Schoenherr, Shashi P. Singh, Maarten van Dinther, Kyle T. Cunningham, Madeleine P. J. White, Tiffany Campion, John Pritchard, et al.(Promega, Cat#E4030). To measure luciferase activity, 30 μL Luciferase Assay Reagent (Promega, Cat# E1483) was added to 20 μL of lysate. The protein concentration of each lysate was analyzed using Bio-Rad protein assay

(15) Design principles to tailor Hsp104 therapeuticsCell ReportsDecember 24, 2024JiaBei Lin, Peter J. Carman, Craig W. Gambogi, Nathan M. Kendsersky, Edward Chuang, Stephanie N. Gates, Adam L. Yokom, Alexandrea N. Rizo, Daniel R. Southworth, James Shorteral.135,N/A pE-His6-SUMO-Ydj1,Michalska et al.135,N/A pFGET19-Ulp1,Addgene,Plasmid #64697 Luciferase Assay Reagent,Promega,Cat# E1483 ATPase Activity Kit (Colorimetric),Innova Bioscieces,Cat# 601–0120 QuikChange Site-Directed Mutagenesis Kit

(16) Ocular toxicity, distribution, and shedding of intravitreal AAV-eqIL-10 in horsesMolecular Therapy - Methods & Clinical DevelopmentDecember 12, 2024Kim Young, Tomoko Hasegawa, Naveen Vridhachalam, Nichol Henderson, Jacklyn H. Salmon, Trace F. McCall, Matthew L. Hirsch, Brian C. Gilger(E194A, Promega, Fitchburg, WI) and tested for luciferase activity using luciferase assay reagent (E1483, Promega). Neutralizing antibody titers were defined as the highest dilution that demonstrated decreased

(17) A peptide derived from sorting nexin 1 inhibits HPV16 entry, retrograde trafficking, and L2 membrane spanningTumour Virus ResDecember 1, 2024Li S, Williamson ZL, Christofferson MA, Jeevanandam A, Campos SK.lysed in 100 μl reporter lysis buffer (RLB, Promega E397A). 100 μl luciferase assay reagent (Promega E1483) was added into 20 μl cell lysate and luciferase activity was measured using a DTX800 Multimode plate

(18) RNA-modifying enzyme Alkbh8 is involved in mouse embryonic developmentiScienceSeptember 20, 2024Manami Nakai, Hiroaki Hase, Yutong Zhao, Katsuya Okawa, Kohei Honda, Kaori Ikuma, Kaori Kitae, Kazutake TsujikawaMix,TOYOBO,QPS-201 Flexi® Rabbit Reticulocyte Lysate System,Promega,Cat#L4540 Luciferase Assay Reagent,Promega,Cat#E1483 BCA Protein Assay,Thermo Fisher SCIENTIFIC,Cat#23227 "Clariom™ S Pico Assay, mouse",Thermo Fisher

(19) Engineered IgM and IgG cleaving enzymes for mitigating antibody neutralization and complement activation in AAV gene transferMolecular therapy : the journal of the American Society of Gene TherapyJuly 3, 2024Smith TJ, Elmore ZC, Fusco RM, Hull JA, Rosales A, Martinez M, Tarantal AF, Asokan A.assessed by measuring luciferase transgene expression with 25 μL Luciferase Assay Reagent (Promega, E1483). Luminescence was measured using a Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific)

(20) Short- and long-term outcomes of pulmonary exposure to a sublethal dose of ricin in miceScientific ReportsMay 21, 2024Anita Sapoznikov, Yentl Evgy, Amir Ben-Shmuel, Arieh Schwartz, Ron Alcalay, Moshe Aftalion, Alon Ben David, Noam Erez, Reut Falachwell was determined by mixing equal volumes of the cell lysate and Luciferase assay reagent (Promega, E1483, Israel) followed by immediate measurement of luminescence levels using Victor3 plate reader (Perkin-Elmer,

Write a Review

(1)

4/16/2019Luciferase Assay Systems (E1483) Liyong Z.Worked very well

Luciferase Assay System by Promega
The Luciferase Assay System from Promega is available in two different formats. You can buy the ... Read More