Nt.BspQI from New England Biolabs

Product Specs
ItemNt.BspQI
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR0644S
Concentration10,000 units/ml
Quantity1000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to convert 1 µg of supercoiled pUC19 DNA to open circular form in 1 hour at 50°C in a total reaction volume of 50 µl.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A non-catalytic role for RFC in PCNA-mediated processive DNA synthesisCellFebruary 19, 2026Gabriella N. L. Chua, Emily C. Beckwitt, Victoria Miller-Browne, Olga Yurieva, Dan Zhang, Bryce J. Katch, Nina Y. Yao, John W. Watters, Kaitlin Abrantes, Ryogo Funabiki, et al.TCEP1 DTT (Dithiothreitol) (> 99% pure) protease free,Goldbio,Cat# DTT10 Nt.BspQI,New England Biolabs,Cat# R0644S PCNA,"Bauer and Burgers, 198857",N/A PCNA-S6,This paper,N/A RFC,"Finkelstein et al., 200340",N/A RFC-S6,This

(2) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce Demplesynthesize the substrate, 100 pmol of N(3–1) Ver 1 X:A was nicked using 120 units of Nt.BspQI (NEB, R0644S) in NEB r3.1 (NEB, B6003S) at 50°C for 1 h in a total reaction volume of 500 μl. Subsequently, 100 units

(3) CLAE: A High‐Fidelity Nanopore Sequencing Strategy for Read‐Level Viral Variant Detection and Environmental RNA Virus DiscoveryAdvanced ScienceNovember 1, 2025Hannah Yu, Sarah Golconda, Ga‐Eun Lee, Dantong Xue, Guillermo Domínguez‐Huerta, James M. Wainaina, Benjamin Bolduc, Shashanka Murthy, Shihyoung Kim, Seth Faith, et al.Nt.BspQI‐, Nt.BbvCI‐, or Nb.BbvCI‐hairpin sequences were then digested with 10 units of Nt.BspQI (NEB R0644S), Nt.BbvCI (NEB R0632S), or Nb.BbvCI (NEB R0631S) nicking enzymes in a 50 µL reaction with enzyme‐specific

(4) Genome integrity sensing by the broad-spectrum Hachiman antiphage defense complexCellNovember 27, 2024Owen T. Tuck, Benjamin A. Adler, Emily G. Armbruster, Arushi Lahiri, Jason J. Hu, Julia Zhou, Joe Pogliano, Jennifer A. Doudna(AMPPNP),Roche,Cat#10102547001 BamHI-HF,New England Biolabs,Cat# R3136T Nt.BspQI,New England Biolabs,Cat#R0644S Proteinase K,New England Biolabs,Cat#P8107S FM4-64,Fisher Scientific,Cat#T13320 "4’,6-diamidino-2-phenylindole,

(5) TEQUILA-seq: a versatile and low-cost method for targeted long-read RNA sequencingNature communicationsAugust 8, 2023Wang F, Xu Y, Wang R, Zhang B, Smith N, Notaro A, Gaerlan S, Kutschera E, Kadash-Edmondson KE, Xing Y, et al.Nickase-induced linear SDA was then performed at 37 °C for 4–16 h using 3 nM (0.04 U/μl) of Nt.BspQI (NEB, #R0644S). Synthesized probes were purified with 1.8× volumes of AMPure XP beads and quantified by NanoDrop 2000

(6) Putative Signals of Generalist Plant Species Adaptation to Local Pollinator Communities and Abiotic FactorsMolecular Biology and EvolutionMarch 4, 2023Léa Frachon, Luca Arrigo, Quint Rusman, Lucy Poveda, Weihong Qi, Giovanni Scopece, Florian P Schiestl80001). In detail, 300 ng of purified gDNA was nicked with Nb.BspQI (New England BioLabs, cat. no. R0644S) in NEB Buffer 3. The nicked DNA was labeled with a fluorescent- deoxyuridine triphosphate (fluorescent-dUTP)

(7) Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subuniteLifeDecember 13, 2022Shoji Tane, Keishi Shintomi, Kazuhisa Kinoshita, Yuko Tsubota, Makoto M Yoshida, Tomoko Nishiyama, Tatsuya Hiranousing Nucleobond PC100 (MACHERREY-NAGEL GmbH & Co KG) was treated with the nicking enzyme Nt.BspQI (R0644S, New England Biolabs). The linearized DNA was prepared by digesting the negatively supercoiled DNA with

(8) Protocol to detect RNAs from tissue sections in mice using Y-branched probe in situ hybridizationSTAR ProtocolsDecember 1, 2022Yunming Wu, C. Ron Yuformamide,VWR,CAT: 97062-008; CAS: 75-12-7 Tissue-Tek O.C.T. Compound,VWR,CAT: 25608-930 nt.BspQi,NEB,CAT: R0644S DMSO,MilliporeSigma,CAT: 276855; CAS: 67-68-5 GelRed Nucleic Acid Gel Stain,Biotium,CAT: 41003 "PBS

(9) DNA mechanical flexibility controls DNA potential to activate cGAS-mediated immune surveillanceNature communicationsNovember 19, 2022Wang L, Li S, Wang K, Wang N, Liu Q, Sun Z, Wang L, Wang L, Liu Q, Song C, et al.Nucleasome protein (Cat. number: 52015) was from BPS bioscience (San Diego, CA, USA). Nt.BspQI (Cat. number: R0644S) was from New England BioLabs (Beijing, PRC). DNA looping assays DNA looping assays were performed

(10) Temperature dependent in vitro binding and release of target DNA by Cas9 enzymeScientific ReportsSeptember 9, 2022Serene Rose David, Sumanth Kumar Maheshwaram, Divya Shet, Mahesh B. Lakshminarayana, Gautam V. Sonicircular and linear forms of this plasmid were generated by nicking the plasmid with Nt.BspQ1 (NEB, R0644S) and digesting with ScaI (NEB, R3122S) or NotI (NEB R0189S) enzymes, respectively. The target sequence

(11) Robust and sensitive in situ RNA detection using Yn-situCell Rep MethodsApril 25, 2022Wu Y, Xu W, Ma L, Yu Z, Wang Y, Yu CR.Compound,VWR,CAT: 25608-930 SfiI,New England Biolabs,CAT: R0123S nt.BspQi,New England Biolabs,CAT: R0644S DMSO,MilliporeSigma,CAT: 276855; CAS: 67-68-5 Midori Green Direct,Bulldog Bio,CAT: MG06 GelRed Nucleic

(12) Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assemblyNature CommunicationsDecember 1, 2021Keishi Shintomi, Tatsuya HiranoDNA substrate, a 3.0-kb plasmid DNA (pBlueScript SK(-)) was treated with the nicking enzyme Nt.BspQI (R0644S, New England Biolabs). The nicked DNA (100 ng) was mixed with 400 ng of recombinant topo IIα in a 10-µl

(13) Single-strand DNA breaks cause replisome disassemblyMolecular CellMarch 1, 2021Kyle B. Vrtis, James M. Dewar, Gheorghe Chistol, R. Alex Wu, Thomas G. W. Graham, Johannes C. Waltercocktail,Roche,Cat# 11873580001 Nb.BbvCI,NEB,Cat# R0631S Nt.BbvCI,NEB,Cat# R0632S Nt.BspQI,NEB,Cat# R0644S AflII,NEB,Cat# R0520S NcoI,NEB,Cat# R0193L MfeI-HF,NEB,Cat# R3589S "Klenow Fragment, exo-",NEB,Cat#

(14) Regulation of DNA Binding and High-Order Oligomerization of the DnaB Helicase LoaderJournal of BacteriologyAugust 17, 2020Lindsay A. Matthews, Lyle A. Simmonsusing BamHI-HF (catalog number R3136S; New England Biolabs) or nicked with Nt.BspQI (catalog number R0644S; New England Biolabs) as previously described (23). EMSAs. The EMSA reaction mixtures (10 ␮l) consisted

(15) Microscale Objects via Restructuring of Large, Double-Stranded DNA MoleculesACS Applied Materials & InterfacesDecember 5, 2018Samuel J. W. Krerowicz, Juan P. Hernandez-Ortiz, David C. Schwartzthen created through the addition of five units of the nicking enzyme Nt.BspQI (New England Biolabs, R0644S) in the presence of 1X NEBuffer 3.1 buffer and incubating at 16 °C for 4 hours. To add the biotin to

(16) Establishment of DNA-DNA Interactions by the Cohesin RingCellJanuary 25, 2018Murayama Y, Samora CP, Kurokawa Y, Iwasaki H, Uhlmann F.1021A XbaI,TaKaRa,Cat# 1093A Nb.BssSI,New England BioLabs,Cat# R0681S Nt. BspQI,New England BioLabs,Cat# R0644S T4 DNA polymerase,TaKaRa,Cat# 2040A E. coli exonuclease I,TaKaRa,Cat# 2650A E. coli exonuclease III,TaKaRa,Cat#

(17) Efficient modification of λ-DNA substrates for single-molecule studiesScientific ReportsDecember 1, 2017Yoori Kim, Armando de la Torre, Andrew A. Leal, Ilya J. Finkelsteinmodified and purified as described above, and 25 μg of the DNA was incubated with 150 U of Nt.BspQI (NEB# R0644S) in a 250 µL reaction with 1X buffer 3.1 (NEB #B7203) at 55 °C for 1 hour. The reaction was halted with

(18) Evolution of a Biomass-Fermenting Bacterium To Resist Lignin PhenolicsApplied and Environmental MicrobiologyJune 1, 2017Tristan Cerisy et al.labeled, repaired, and stained (NLRS) DNA. Brieﬂy, 300 ng of DNA was nicked with 10 U Nt.BspQI (NEB R0644S) for 2 h at 37°C. Nicked DNA was incubated for 1 h at 72°C with ﬂuorescently labeled Alexa 546-dUTP

(19) Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of DNA Mismatch RepairMethods in enzymologyJanuary 1, 2017Brown MW, de la Torre A, Finkelstein IJ.electrophoresis (Fig. 3c). In a 250 μL reaction, incubate 25 μg of recombinant λ-DNA with 50 U of Nt.BspQI (NEB #R0644s) and 1× buffer 3.1 (NEB #B7203) at 55°C for 1 h. Add 1 U of proteinase K (NEB #P8107S) for 1 h, at 55°C

(20) Reconstituting ParA/ParB-mediated transport of DNA cargoMethods in Cell BiologyMay 23, 2015Anthony G. Vecchiarelli, James A. Taylor, Kiyoshi Mizuuchiplasmid (4.4 Kb) pBR322::sopC plasmid (4.7 Kb) Nt BspQI Nicking Enzyme (New England Biolabs, Cat. # R0644S) Phenol:CHCl3:Isoamyl Alcohol (UltraPure 25:24:1 v/v) Ethanol Deoxyribonucleotide (dNTP) mix (Life Technologies

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Nt.BspQI

Nt.BspQI from New England Biolabs

Description

Specifications/Features

New England Biolabs

Citations (20)

Reviews

Nt.BspQI from New England Biolabs

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