Nb.BtsI from New England Biolabs

Product Specs
ItemNb.BtsI
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR0707S
Concentration10,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C
Quantity1000 units

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to convert 1 µg of supercoiled plasmid ΦX174 RF I DNA to open circular form in 1 hour at 37°C in a total reaction volume of 50 µl.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A denaturation-free protocol for in situ visualization of short nuclear DNA sequences using padlock probes with rolling-circle amplificationPLoS OneOctober 28, 2025Ryoyo Ikebuchi, Lu Xi, Dimitra Bouri, Valeriia Svintytska, Hanna Davies, Pawel Olszewski, Bozena Bruhn-Olszewska, Jan P. Dumanski, Ulf Landegrenrelax the supercoiled dsDNA, M13mp18 RF I DNA (N4018S, New England Biolabs) was nicked with Nb. BtsI (R0707S, New England Biolabs) according to the manufacturer’s protocol and stored at −20°C until use. One nM

(2) Assessing species-specific neonicotinoid toxicity using cross-species chimeric nicotinic acetylcholine receptors in a Drosophila modelSci RepOctober 16, 2025Anna Lassota, James J. L. Hodge, Matthias Soller(nAChRβ2Amα8LBD). The pUC19 pBac w + Amelα8/Dβ2 plasmid was treated with the nicking endonuclease Nb.BtsI (NEB, R0707S) prior to injection. Transgenic lines were generated by injection into a GFP nosCas9 carrying flies

(3) Efficient assembly of long DNA fragments and multiple genes with improved nickase‐based cloning and Cre/ loxP recombinationPlant Biotechnology JournalOctober 1, 2022Yanchang Zhao et al.with 5 U Nb.BsrDI (NEB catalogue no. R0648S, USA) at 65 °C for 1 h, or 5 U Nb.BtsI (NEB catalogue no. R0707S, USA) at 37 °C for 1 h, and then heat inactivated at 70 °C for 30 min (also melted out the nicked short

(4) Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reportersNature ProtocolsSeptember 1, 2021C. G. Piett, T. J. Pecen, D. J. Laverty, Z. D. NagelVV-00141-03) Appropriate single strand nicking enzyme: Nt.BspQI (NEB, cat. no. RO644S) Nb.BtsI (NEB, cat. no. R0707S), Alternative restriction enzyme which targets only one strand of the DNA DNase/RNase free microcentrifuge

(5) High density of unrepaired genomic ribonucleotides leads to Topoisomerase 1-mediated severe growth defects in absence of ribonucleotide reductaseNucleic acids researchMay 7, 2020Cerritelli SM, Iranzo J, Sharma S, Chabes A, Crouch RJ, Tollervey D, El Hage A.(∼0.5 μg) were incubated in 1× CutSmart buffer in presence of 2.5 u of Nb.BtsI (New England Biolabs R0707S), either in the absence or presence of 0.1 μg μl−1 RNase A, in a total volume of 50 μl, at 37°C for

(6) Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expressionNature communicationsNovember 29, 2019Montaldo NP, Bordin DL, Brambilla A, Rösinger M, Fordyce Martin SL, Bjørås KØ, Bradamante S, Aas PA, Furrer A, Olsen LC, et al.generated as follows; single-stranded DNA was obtained by nicking the plasmid pmax:mPlum with Nb.BtsI (NEB R0707S) for 4 h at 37 °C in NEB Buffer 4 (NEB B7004). After phenol chloroform extraction the DNA was incubated

(7) Quantification of cytosolic plasmid DNA degradation using high-throughput sequencing: implications for gene deliveryThe Journal of Gene MedicineMay 28, 2014Rahul Rattan, Anna U. Bielinska, Mark M. Banaszak HollR0187S), were obtained from New England BioLabs, Inc. (Beverly, MA, USA). Nb.BtsI (catalogue number R0707S) and Nt.CviPII (catalogue number R0626S) were obtained from New England BioLabs Inc. An Illumina HiSeq

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Nb.BtsI

Nb.BtsI from New England Biolabs

Specifications/Features

New England Biolabs

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Nb.BtsI from New England Biolabs

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