Nt.CviPII from New England Biolabs

Product Specs
ItemNt.CviPII
CompanyNew England Biolabs
Price $141.00 Inquire
Catalog NumberR0626S
Concentration3,000 units/ml
Quantity40 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 µg of pUC19 DNA resulting in a stable pattern of fragments between 25 and 200 bp in 1 hour at 37°C in a total reaction volume of 50 µl.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Altered chromatin accessibility and nucleosome positioning landscape upon HDAC and LSD1 inhibition in cancer cellbioRxivApril 11, 2026Sagnik Sen, Pierre O. Estève, Devesh Tarasia, Rachel Danneberg, Ashmita Dey, Ujjwal Maulik, Sanghamitra Bandyopadhyay, Sriharsa Pradhanchromatin DNA labeling was performed by incubating the nuclei in the presence of 2 U of Nt.CviPII (NEB R0626S), 10 U of DNA polymerase I (M0209S) and 30 μM of each dNTP, including 6 μM of Fluorescein-12-dATP (Perkin Elmer

(2) Normozoospermic infertile men possess subpopulations of sperm varying in DNA accessibility, relating to differing reproductive outcomesHuman ReproductionJuly 1, 2025Mark E Gill, Manuel Fischer, Christian De Geyter, Antoine H F M PetersNo. R0181), 6 µM Fluorescein-dATP (Jena Biosciences, No. NU-1611-FAMX), 3.2 U/ml Nt.CviPII (NEB No. R0626S), and 64 U/ml E. coli DNA Polymerase I (NEB No. M0209L). For Nick translation, the nicking endonuclease

(3) Distinct structural and functional heterochromatin partitioning of lamin B1 and lamin B2 revealed using genome-wide nicking enzyme epitope targeted DNA sequencingNucleic Acids ResJanuary 16, 2025Sagnik Sen, Pierre-Olivier Estève, Karthikeyan Raman, Julie Beaulieu, Hang Gyeong Chin, George R Feehery, Udayakumar S Vishnu, Shuang-Yong Xu, James C Samuelson, Sriharsa PradhanT3050). After DNA purification, genomic DNA was cleaved (digested) using two units of Nt.CviPII (NEB # R0626S) in 500 μl of 1X NEBufferTM 2 at 37°C for 4 h to overnight (This ensures the nick translated 5mC conjugated

(4) NicE-C efficiently reveals open chromatin-associated chromosome interactions at high resolutionGenome researchMarch 1, 2022Luo Z, Zhang R, Hu T, Zhu Y, Wu Y, Li W, Zhang Z, Yao X, Liang H, Song X.800g for 3 min at 4°C. The pellet was resuspended in the following reaction: 0.5 μL of Nt.CviPII (NEB R0626S), 2 μL of DNA Polymerase I (NEB M0209L), 3 μL of 1 mM dTTP, dGTP, biotin-14-dATP (Jena Bioscience NU-835-BIO14-S)

(5) One-pot universal NicE-seq: all enzymatic downstream processing of 4% formaldehyde crosslinked cells for chromatin accessibility genomicsEpigenetics & chromatinDecember 11, 2021Vishnu US, Estève PO, Chin HG, Pradhan S.chromatin DNA labeling was performed by incubating the nuclei in the presence of 2.5 U of Nt.CviPII (NEB R0626S), 50 U of DNA polymerase I (M0209S) and 30 μM of each dNTP including 6 μM of Fluorescein-12-dATP (Perkin Elmer

(6) Universal NicE-seq for high-resolution accessible chromatin profiling for formaldehyde-fixed and FFPE tissuesClinical EpigeneticsDecember 1, 2020Hang Gyeong Chin, Zhiyi Sun, Udayakumar S. Vishnu, Pengying Hao, Paloma Cejas, George Spracklin, Pierre-Olivier Estève, Shuang-yong Xu, Henry W. Long, Sriharsa Pradhanchromatin DNA was labeled with biotin by incubating the nuclei in the presence of 2.5 U of Nt.CviPII (NEB, R0626S), 50 U of DNA polymerase I (NEB, M0209S) and 30 μM of each dGTP and dTTP, 24 μM of 5-mdCTP (NEB, NO356S)

(7) Northern lights assay: a versatile method for comprehensive detection of DNA damageNucleic Acids ResearchJuly 24, 2018Bjarki Gudmundsson et al.using either a range of 0.1–1 U Nt.BstNB I (NEB, cat no. R0607S) or 0.008–10 U Nt.CviPII (NEB, cat no. R0626S) in buffer NEB 3 at 55°C for 1 h in total volume of 50 μl. Cy5-labeling of DNA ladder GeneRuler 100

(8) NicE-seq: high resolution open chromatin profilingGenome BiologyDecember 1, 2017V. K. Chaithanya Ponnaluri, Guoqiang Zhang, Pierre-Olivier Estève, George Spracklin, Stephanie Sian, Shuang-yong Xu, Touati Benoukraf, Sriharsa Pradhanchromatin DNA was labeled with biotin by incubating the nuclei in the presence of 2.5 U of Nt.CviPII (NEB R0626S), 10 U of DNA polymerase I (M0209S) and 30 μM of each dNTP including 6 μM of biotin-14-dATP (Invitrogen,

(9) Quantification of cytosolic plasmid DNA degradation using high-throughput sequencing: implications for gene deliveryThe Journal of Gene MedicineMay 28, 2014Rahul Rattan, Anna U. Bielinska, Mark M. Banaszak HollNew England BioLabs, Inc. (Beverly, MA, USA). Nb.BtsI (catalogue number R0707S) and Nt.CviPII (catalogue number R0626S) were obtained from New England BioLabs Inc. An Illumina HiSeq 2000 (Illumina, Inc., San Diego, CA,

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Nt.CviPII

Nt.CviPII from New England Biolabs

Specifications/Features

New England Biolabs

Citations (9)

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Nt.CviPII from New England Biolabs

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