Lung Dissociation Kit, mouse from Miltenyi Biotec

(1) Modulation of recovery from neonatal hyperoxic lung injury by sex as a biological variableRedox BiologyDecember 1, 2023Abiud Cantu, Manuel Cantu Gutierrez, Xiaoyu Dong, Connor Leek, Montserrat Anguera, Krithika Lingappanand homogenized and dissociated using the Miltenyi MACS lung dissociation kit (Miltenyi Biotec, cat # 130-095-927), using the GentleMacs program m-Lung-1 on the AUTO MaCs. The lungs were incubated for 20min at 37 °C

(2) Furin Regulates the Alveolarization of Neonatal Lungs in a Mouse Model of Hyperoxic Lung InjuryBiomoleculesNovember 16, 2023Shin Kato, Osuke Iwata, Hiroyuki Kato, Satoko Fukaya, Yukari Imai, Shinji Saitohby the mincing of the peripheral lung parenchyma, and processing using a lung cell dissociation kit (130-095-927, Miltenyi, Auburn, CA, USA) at 37 °C for 30 min with agitation. The lung digests were centrifuged at

(3) Glutamine metabolism inhibition has dual immunomodulatory and antibacterial activities against Mycobacterium tuberculosisNature CommunicationsNovember 16, 2023Sadiya Parveen, Jessica Shen, Shichun Lun, Liang Zhao, Jesse Alt, Benjamin Koleske, Robert D. Leone, Rana Rais, Jonathan D. Powell, John R. Murphy, et al.individual lobes, and single-cell suspension was prepared using the mouse lung dissociation kit (Cat: 130-095-927; Miltenyi Biotec, Gaithersburg, MD) and GentleMACS™ Dissociator following the manufacturer’s protocol.

(4) Intravenous BCG vaccination reduces SARS-CoV-2 severity and promotes extensive reprogramming of lung immune cellsiScienceOctober 20, 2023Singh AK, Wang R, Lombardo KA, Praharaj M, Bullen CK, Um P, Gupta M, Srikrishna G, Davis S, Komm O, et al.used in single cell preparation and single cell RNA sequencing We used Mouse lung dissociation kit (130-095-927) and Mouse spleen dissociation kit (130-095-926) that were purchased from Miltenyi Biotec and are commercially

(5) Nr4a1 marks a distinctive ILC2 activation subset in the mouse inflammatory lungBMC BiologyOctober 13, 2023Shasha Xu, Yu Zhang, Xingjie Liu, Huisheng Liu, Xinya Zou, Linlin Zhang, Jing Wang, Zhiwei Zhang, Xiang Xu, Mingxia Li, et al.the lung were prepared according to the instructions of the Lung Dissociation kit (Miltenyi Biotec, 130–095-927). The tissues were digested in a shaking water bath at 37 °C for 12 min. Flow cytometry assay and cell sorting

(6) Combinatorial design of nanoparticles for pulmonary mRNA delivery and genome editingNature BiotechnologyOctober 1, 2023Bowen Li, Rajith Singh Manan, Shun-Qing Liang, Akiva Gordon, Allen Jiang, Andrew Varley, Guangping Gao, Robert Langer, Wen Xue, Daniel Andersonsingle cells The single cells were generated with the lung dissociation kit (Miltenyi Kit: Catalog #: 130-095-927). Briefly, the mouse lung was perfused with PBS and placed in a 10cm cell culture dish. The lung tissue

(7) Blockade of IL-10 Signaling Ensures Mifamurtide Efficacy in Metastatic OsteosarcomaCancers (Basel)September 27, 2023Nicoletta Nastasi, Amada Pasha, Gennaro Bruno, Angela Subbiani, Laura Pietrovito, Angela Leo, Lucia Scala, Lorena de Simone, Gabriella Casazza, Federica Lunardi, et al.24 h, mice were sacrificed, lungs isolated, and dissociated with the Lung Dissociation Kit mouse (#130-095-927, Miltenyi Biotec®). In the staining protocol, during IL-6 and IL-10 blockade, Fc blocking reagents were

(8) Hyperactive KRAS/MAPK signaling disrupts normal lymphatic vessel architecture and functionFrontiers in Cell and Developmental BiologySeptember 25, 2023Lorenzo M. Fernandes, Jeffrey Tresemer, Jing Zhang, Jonathan J. Rios, Joshua P. Scallan, Michael T. Dellinger(Miltenyi Biotec, #130-093-237), and then digested with a mouse lung dissociation kit (Miltenyi Biotec, #130-095-927). A gentleMACS tissue dissociator was used to create a single-cell suspension. CD31 microbeads (Miltenyi Biotec

(9) Immunotherapy targeting different immune compartments in combination with radiation therapy induces regression of resistant tumorsNat CommunAugust 24, 2023Nils-Petter Rudqvist, Maud Charpentier, Claire Lhuillier, Erik Wennerberg, Sheila Spada, Caroline Sheridan, Xi Kathy Zhou, Tuo Zhang, Silvia C. Formenti, Jennifer S. Sims, et al.Dissociation Kit (Miltenyi Biotec, cat #130-096-730) and Lung Dissociation Kit (Miltenyi Biotec, cat #130-095-927) on a gentleMACS Octo Dissociator (Miltenyi Biotec). Tissue homogenates were resuspended in 10% FBS-RPMI-1640

(10) Streptococcus pneumoniae drives specific and lasting Natural Killer cell memoryPLOS PathogensJuly 24, 2023Tiphaine M. N. Camarasa, Júlia Torné, Christine Chevalier, Orhan Rasid, Melanie A. Hamon(Miltenyi Biotec) in cTubes containing lung dissociation kit reagents (DNAse and collagenase ref no. 130-095-927, Miltenyi Biotec). Lungs, BALF, nasal lavage, blood, spleen homogenates were plated in serial dilutions

(11) Monitoring Group 2 Innate Lymphoid Cell Biology in Models of Lung InflammationBIO-PROTOCOLJuly 20, 2023Jana H. Badrani, Allyssa N. Strohm, Yung-An Huang, Taylor A. Doherty14-959-5)C57BL/6 mice (6–8 weeks old; female)Miltenyi Lung Digest kit (Miltenyi Biotec, catalog number: 130-095-927)Mouse recombinant IL-33 (Thermo Fisher, catalog number: 14-8332-80)Alternaria alternataextract (Fisher

(12) Single-cell transcriptome sequencing–based analysis: probing the mechanisms of glycoprotein NMB regulation of epithelial cells involved in silicosisParticle and Fibre ToxicologyJuly 19, 2023Shaoqi Yang, Yuheng Sun, Min Long, Xinbei Zhou, Mengqin Yuan, Liliang Yang, Wei Luo, Yusi Cheng, Xinxin Zhang, Wei Jiang, et al.approximately 10-mm pieces and dissociated into single cells using a Lung Dissociation Kit (Miltenyi Biotech, 130-095-927, Germany). Using Single-Cell 5’ Library and Gel Bead Kit (10x Genomics, 1,000,169) and Chromium Single-Cell

(13) Multi-omic assessment shows dysregulation of pulmonary and systemic immunity to e-cigarette exposureRespiratory ResearchMay 25, 2023David P. Scieszka, Devon Garland, Russell Hunter, Guy Herbert, Selita Lucas, Yan Jin, Haiwei Gu, Matthew J. Campen, Judy L. Cannonmagnesium until batch dissociation could be performed. The Miltenyi Biotec Lung Dissociation Kit (cat. No. 130-095-927) was employed, and the associated protocol followed, as it has been developed to minimize cell death

(14) Endothelial cell senescence exacerbates pulmonary hypertension by inducing juxtacrine Notch signaling in smooth muscle cellsiScienceMay 19, 2023Risa Ramadhiani, Koji Ikeda, Kazuya Miyagawa, Gusty Rizky Tough Ryanto, Naoki Tamada, Yoko Suzuki, Yuhei Kirita, Satoaki Matoba, Ken-ichi Hirata, Noriaki EmotoInc.). After 30 min incubation, homogenization of lungs were performed using gentleMACS Dissociators (#130-095-927, Miltenyi Biotec Inc.). The homogenate was filtered sequentially through 70 μm MACS Smart Strainer and

(15) Single cell transcriptomics clarifies the basophil differentiation trajectory and identifies pre-basophils upstream of mature basophilsNature CommunicationsMay 18, 2023Kensuke Miyake, Junya Ito, Jun Nakabayashi, Shigeyuki Shichino, Kenji Ishiwata, Hajime Karasuyamausing gentleMACS dissociator (Miltenyi Biotec) and Lung Dissociation Kit (Miltenyi Biotec, catalog #: 130-095-927) or Multi Tissue Dissociation Kit 1 (Miltenyi Biotec, catalog #: 130-110-201). Cells were stained with

(16) Extracellular Vesicle ITGAM and ITGB2 Mediate Severe Acute Pancreatitis-Related Acute Lung InjuryACS nanoApril 25, 2023Hu Q, Zhang S, Yang Y, Li J, Kang H, Tang W, Lyon CJ, Wan M.method. Single cells were prepared from mouse lung tissue using a mouse lung dissociation kit (cat. no.130-095-927, Miltenyi Biotec). CD45-CD90.2-CD326 cells were screened by depletion with CD45 MicroBeads (cat. no.

(17) LMAN1 is a receptor for house dust mite allergensCell reportsMarch 28, 2023Miller MH, Swaby LG, Vailoces VS, LaFratta M, Zhang Y, Zhu X, Hitchcock DJ, Jewett TJ, Zhang B, Tigno-Aranjuez JT.digested and homogenized using a lung dissociation kit and GentleMacs (Miltenyi Biotec; Auburn, CA) (Cat # 130-095-927) and stained with PE anti-mouse LMAN1 (OTI1A8) and APC anti-mouse CD326 (G8.8). All cells were blocked

(18) Age-associated microenvironmental changes highlight the role of PDGF-C in ER+ breast cancer metastatic relapseNature CancerMarch 13, 2023Frances K. Turrell, Rebecca Orha, Naomi J. Guppy, Andrea Gillespie, Matthew Guelbert, Chris Starling, Syed Haider, Clare M. Isackelungs were dissociated using the mouse tumor or lung dissociation kit (Miltenyi Biotec, 130-096-730 or 130-095-927), collected in Buffer S and dissociated into single-cell suspensions using the gentleMACS Octo Dissociator

(19) Diversity of arterial cell and phenotypic heterogeneity induced by high-fat and high-cholesterol dietFrontiers in Cell and Developmental BiologyFebruary 23, 2023Jieqi Wen, Rongsong Ling, Ruiyue Chen, Siyan Zhang, Yarong Dai, Tingtao Zhang, Fanyu Guo, Qingxin Wang, Guixin Wang, Yizhou JiangThe aorta were then harvested and incubated using the enzyme mix in the Lung Dissociated Kit (Cat# 130-095-927, Miltenyi Biotec). The aortic arch was cut into pieces before being immersed with the enzyme mix, and

(20) Macrophage-derived GPNMB trapped by fibrotic extracellular matrix promotes pulmonary fibrosisCommunications biologyFebruary 2, 2023Wang J, Zhang X, Long M, Yuan M, Yin J, Luo W, Wang S, Cai Y, Jiang W, Chao J.(approximately 1 mm) and dissociated into single cells using a Lung Dissociation Kit (Miltenyi Biotech, 130-095-927, Germany). With the Single-Cell 5’ Library and Gel Bead Kit (10x Genomics, 1000169) and Chromium Single-Cell

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5/5/2016Good for Brain Tissue Dissociation Jesús CirizaWe have dissociated lung tissue with this kit and it works very well.
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4/12/2016Acceptable Level of Single Cell Isolation from Lung Sivaraman NatarajanThe lung Dissociation kit yielded decent number single cells from the mouse lung following protocol comes with kit. We used this kit to study isolated the single cells from the mouse lung for ... Read Review

Lung Dissociation Kit, mouse (for 50 preparations)

Lung Dissociation Kit, mouse from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Lung Dissociation Kit, mouse from Miltenyi Biotec

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