Liver Digest Medium from Thermo Fisher Scientific

Product Specs
ItemLiver Digest Medium
CompanyThermo Fisher Scientific
Price $173.00Supplier Page View Company Product Page
Catalog Number17703034
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Quantity1 unit
TypeMedium

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) mRNA vaccination overcomes haemozoin-mediated impairment of whole-parasite malaria vaccines in miceNature MicrobiologyFebruary 2, 2026Mariah Hassert, Lisa L. Drewry, Lecia L. Pewe, Lisa S. Hancox, Rui He, Sahaana Arumugam, Madison R. Mix, Aliasger K. Salem, John T. Hartyminced by GentleMACS processing (Miltenyi) and then digested in liver digest medium (Thermo Fisher 17703034). Homogenized livers were pushed through 100-µm filters, and hepatocytes were collected by pelleting

(2) The origin of hepatocellular carcinoma depends on metabolic zonationScienceJanuary 29, 2026Jason Guo, Roger Liang, Andrew Chung, Zhijie Li, Boyuan Li, Eric Chen, Lin Li, Jingjing Wang, Meng-Hsiung Hsieh, Ivy Xiangyi Fang, et al.Perfusion Medium (Life Technologies #17701038) followed by 30 ml of Liver Digest Medium (Life Technologies #17703034). The liver cells were suspended in 10 ml of Liver Digest Medium and filtered through a 70 μM cell strainer.

(3) Liver sinusoidal endothelial cells constitute a major route for hemoglobin clearanceEMBO ReportsJanuary 6, 2026Gabriela Zurawska, Zuzanna Sas, Aneta Jończy, Raghunandan Mahadeva, Patryk Slusarczyk, Marta Chwałek, Daniel Seehofer, Georg Damm, Rafał Mazgaj, Marcin Skórzyński, et al.the portal vein. Next, the medium was exchanged for prewarmed (37 °C) Liver Digest Medium (Gibco, #17703034), and perfusion continued for the next 15 min. Digested livers were gently disintegrated in Hepatocyte Wash Medium

(4) Cysteine 129 in Pla2g12b Is Critical for Intestinal and Hepatic Lipoprotein Secretion in MiceCellular and Molecular Gastroenterology and HepatologyJanuary 1, 2026Binu Prakash, Sujith Rajan, Bhargavi Gangula, Thomas Palaia, Chandana Prakashmurthy, Pradeep Kumar Yadav, Swati Valmiki, Xiaoyue Pan, M. Mahmood Hussain#17701038) containing 1% penicillin/streptomycin mixture, followed by 35 mL liver digestion medium (Gibco, #17703034) containing 0.25% collagenase II (Thermo Fisher Scientific, #17101015). The liver was collected and

(5) Adipose microsomal triglyceride transfer protein deficiency protects against hepatic steatosis by upregulating PPARα activityJHEP ReportsDecember 1, 2025Sujith Rajan, Michael Verano, Thomas Palaia, Chandana Prakashmurthy, Jay Chung, Shahidul Islam, Lili Lee, Antonisamy William James, José O. Alemán, Ira J. Goldberg, et al.#17701038) containing 1% penicillin/streptomycin followed by 35 ml liver digestion medium (Gibco, #17703034) containing 0.25% collagenase II (Thermo Fisher Scientific, #17101015). The liver was collected and

(6) The G1/S transition in mammalian stem cells in vivo is autonomously regulated by cell sizeNat CommunOctober 13, 2025Shicong Xie, Shuyuan Zhang, Gustavo de Medeiros, Prisca Liberali, Jan M. Skotheimperfusion medium (Thermo Fisher Scientific, 17701038), liver digest medium (Thermo Fisher Scientific, 17703034) and hepatocyte wash medium (Thermo Fisher Scientific, 17704024). Unless otherwise noted, cells were

(7) TYK2 Promotes Immunosurveillance of Colorectal Cancer Liver MetastasisCancer ResearchSeptember 24, 2025Bernadette Mödl, Daniela Zwolanek, Katharina Schwertner, Dana Krauß, Stefan Moritsch, Irene Scharf, Anne-Sophie Ebner, Veronica Moreno-Viedma, Cristiano de Sa Fernandes, Philipp Novoszel, et al.(17701038, Thermo Fisher Scientific) to flush out blood, followed by further liver digestion medium (17703034, Thermo Fisher Scientific). The digested liver tissues were ground and passed into a 50 ml tube through

(8) HBO1 functions as an epigenetic barrier to hepatocyte plasticity and reprogramming during liver injuryCell Stem CellJune 5, 2025Wei-Chien Yuan, Andrew S. Earl, Sai Ma, Karel Alcedo, Jacquelyn O. Russell, Fabiana M. Duarte, Yen-Ting Chu, Pei-Chi Chang, Hsin-Yi Chen, Hsin-Hui Chi, et al."HBSS, no calcium, no magnesium",Gibco,Cat#14170112 Collagenase Type IV,Invitrogen,Cat#17104019 Liver digest medium,Gibco,Cat#17703034 Accutase,Millipore,Cat#SCR005 Optiprep,Axis-Shield,Cat#1114542 0.25% Trypsin-EDTA,Gibco,Cat#25200–114 RBC

(9) Multi-zonal liver organoids from human pluripotent stem cellsNatureMay 29, 2025Hasan Al Al Reza, Connie Santangelo, Kentaro Iwasawa, Abid Al Al Reza, Sachiko Sekiya, Kathryn Glaser, Alexander Bondoc, Jonathan Merola, Takanori Takebepieces were chopped into a fine paste like consistency and submerged in Liver Digest Medium (Gibco, 17703034) for 15 min at 37 °C to isolate single cells44. The cells were then passed through a 100-μm strainer

(10) Hepatocyte-specific CLSTN3B ablation impairs lipid droplet maturation and alleviates diet-induced steatohepatitis in micebioRxivMay 28, 2025Chuanhai Zhang, Dengbao Yang, Hiroyuki Suzuki, Jingxuan Chen, Jingjing Wang, Mengchen Ye, Jin Zhou, Qiyu Zeng, Meijuan Bai, Mei-Jung Lin, et al.with Liver Perfusion Medium, the liver was perfused with Liver Digestion Medium (Life Technologies, #17703034) at the same flow rate, using an additional 30–50 mL. After digestion, the liver was carefully removed

(11) Harnessing macrophage-drug conjugates for allogeneic cell-based therapy of solid tumors via the TRAIN mechanismNature communicationsFebruary 4, 2025Taciak B, Bialasek M, Kubiak M, Marszalek I, Gorczak M, Osadchuk O, Kurpiel D, Strzemecki D, Barwik K, Skorzynski M, et al.vein transection. The medium was then replaced with pre-warmed (37 °C) Liver Digest Medium (Gibco, 17703034), and perfusion continued for another 15 min. Digested livers were disaggregated in Hepatocyte Wash Medium

(12) Lithocholic acid phenocopies anti-ageing effects of calorie restrictionNatureDecember 18, 2024Qi Qu, Yan Chen, Yu Wang, Shating Long, Weiche Wang, Heng-Ye Yang, Mengqi Li, Xiao Tian, Xiaoyan Wei, Yan-Hui Liu, et al.from Thermo: DMEM-high glucose (12800082), Liver Perfusion Medium (17701038), Liver Digest Medium (17703034), William’s E Medium (12551032), Ham’s F-10 medium (11550043), basic fibroblast growth factor (bFGF;

(13) Reciprocal tumor‐platelet interaction through the EPHB1‐EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinomaCancer CommunicationsDecember 8, 2024Lin‐Li Yao, Wei‐Ting Qin, Li‐Peng Hu, Tie‐Zhu Shi, Jian Yu Yang, Qing Li, Hui‐Zhen Nie, Jun Li, Xu Wang, Lei Zhu, et al.liver was perfused using a perfusion medium (#17701038, Gibco), dissociated with Liver Digest Medium (#17703034, Gibco) and filtered through a 30‐µm strainer. Single‐cell suspensions were centrifuged at 60 ×g at

(14) Genome dilution by cell growth drives starvation-like proteome remodeling in mammalian and yeast cellsNature Structural & Molecular BiologyDecember 1, 2024Michael C. Lanz, Shuyuan Zhang, Matthew P. Swaffer, Inbal Ziv, Luisa Hernández Götz, Jacob Kim, Frank McCarthy, Daniel F. Jarosz, Joshua E. Elias, Jan M. Skotheimperfusion medium (Thermo Fisher Scientific, 17701038), liver digest medium (Thermo Fisher Scientific, 17703034), and hepatocyte wash medium (Thermo Fisher Scientific, 17704024) were used for this process. After

(15) Selection promotes age-dependent degeneration of the mitochondrial genomebioRxivOctober 3, 2024Ekaterina Korotkevich, Daniel N. Conrad, Zev J. Gartner, Patrick H. O’Farrellwith liver perfusion medium (Gibco, 17701038) for 3 minutes and then with liver digest medium (Gibco, 17703034) for 7 minutes using a peristaltic pump (Gilson, Minipuls 3). Pump was set to 4.4 ml per minute and

(16) Etomoxir repurposed as a promiscuous fatty acid mimetic chemoproteomic probeiScienceSeptember 20, 2024Choi J, Smith DM, Lee YJ, Cai D, Hossain MJ, O'Connor TJ, Deme P, Haughey NJ, Scafidi S, Riddle RC, et al.performed with 25mL of 0.5mM EGTA in PBS, 25mL of PBS, and then 20mL of Liver Digest Media (Gibco, 17703034) supplemented with 0.15 mg/mL collagenase (Sigma, C5138) at a rate of 2 mL/min. Perfused livers were

(17) Remodelling of the translatome controls diet and its impact on tumorigenesisNatureSeptember 5, 2024Haojun Yang, Vincenzo Andrea Zingaro, James Lincoff, Harrison Tom, Satoshi Oikawa, Juan A. Oses-Prieto, Quinn Edmondson, Ian Seiple, Hardik Shah, Shingo Kajimura, et al.rate of 5 mL/min for three minutes, subsequently followed by digestion with Liver Digest Medium (GIBCO 17703034) for seven minutes. All solutions were maintained at a temperature of 37°C for the duration of the perfusion

(18) Self-Assembled Generation of Multi-zonal Liver Organoids from Human Pluripotent Stem CellsbioRxivSeptember 4, 2024Hasan Al Reza, Connie Santangelo, Abid Al Reza, Kentaro Iwasawa, Sachiko Sachiko, Kathryn Glaser, Alexander Bondoc, Jonathan Merola, Takanori Takebepieces were chopped into a fine paste like consistency and submerged in Liver Digest Medium (Gibco, 17703034) for 15 min at 37°C to isolate single cells. The cells were then passed through a 100 μm strainer on

(19) PKD1 mutant clones within cirrhotic livers inhibit steatohepatitis without promoting cancerCell MetabolismAugust 6, 2024Min Zhu, Yunguan Wang, Tianshi Lu, Jason Guo, Lin Li, Meng-Hsiung Hsieh, Purva Gopal, Yi Han, Naoto Fujiwara, Darren P. Wallace, et al.liver perfusion medium (Life Technologies #17701038) and liver digestion medium (Life Technologies # 17703034) and spun down at 50 g for 5 min at 4°C. Hepatocytes were washed twice with hepatocyte washing medium

(20) HSDL2 links nutritional cues to bile acid and cholesterol homeostasisScience AdvancesMay 31, 2024Nolwenn Samson, Cristina R. Bosoi, Christian Roy, Laurie Turcotte, Laura Tribouillard, Mathilde Mouchiroud, Line Berthiaume, Jocelyn Trottier, Heitor C. G. Silva, Thomas Guerbette, et al.perfused and digested using liver perfusion medium (Gibco, #17701038) and liver digestion medium (Gibco, #17703034), respectively. Livers were then collected, disassembled, filtered, and lastly centrifuged with Percoll

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Liver Digest Medium from Thermo Fisher Scientific

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Liver Digest Medium from Thermo Fisher Scientific

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