Fig 1: T cell-specific mRNA expression levels and cytokine secretion by mice with GVHD(A–C) Levels of mRNAs encoding (A) Th1, (B) Th17, and (C) the Treg cell transcription factor were determined via real-time PCR on day 35 after BMT. The control and DSC-EV-injected groups exhibited lower levels of mRNAs encoding T-bet and Rorc than did the other groups, but higher levels of mRNA encoding Foxp3. The GVHD group and the group injected with DSC-IL-6i exhibited the opposite behavior.(D–F) Serum levels of secreted inflammatory cytokines and chemokines measured via ELISA 35 days after BMT. The GVHD group and the DSC-IL-6i-injected group exhibited higher levels than did the other groups. The results are representative of those of two independent experiments. The columns represent the mean values of three independent experiments and the error bars represent SDs. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.