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Polymerase Chain Reaction (PCR)
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Random Primers
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Random Primers
Random Primers
Random Primers from Promega
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Citations:
(246)
Figures:
(2)
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Description
Use for first-strand cDNA synthesis with a reverse transcriptase
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Random Primers
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
C1181
Target
Random
Quantity
20microg
Primer Type
Random Primers
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (246)
(1)
TCR γδ cell-specific STAT5 gain of function induces a druggable chronic human immune dysregulation
J Hum Immun
September 7, 2026
Benedikt J. Meyer, José Pedro Loureiro, Vladimir Nosi, Robin Hupfer, Adhideb Ghosh, Fabio Poletti, Annaïse Jauch, Julia Hirsiger, Caroline Berkemeier, Ingmar Heijnen,
et al.
Concentrations of 100 ng/μl to 1 μg/μl RNA were used for
cDNA synthesis
. We annealed random primers (#
C1181
;
Promega
) at 70°C for 5 min.
cDNA synthesis
was done according to the Qiagen GoScript Reverse Transcription
(2)
Embryonic thermal programming combined with fractionated feeding induces early activation of hepatic lipid storage pathways in mule ducks
Poultry Science
September 1, 2026
Laura-Lou Zwick, Joséphine Huot, Cécile Heraud, Marie Lasserre, Anne Surget, Karine Gontier, Jérôme Roy, Stéphane Panserat, Marianne Houssier
µl RNase-free water. The reaction included
Random Primers
(
Promega
, Madison, WI; product reference:
C1181
, 0.2 µg/ml final), a
PCR
nucleotides mix (
Promega
, Madison, WI; product reference:
C1145
, 0.004 mM final),
(3)
Growth Plate Morphogens as Potential Modulators of Cellular Tissue Remodeling by Annulus Fibrosus Cells
JOR Spine
August 22, 2026
K. Stradovnik-Poikilidis, M. Teunissen, F. M. Riemers, M. van Doeselaar, J. Foolen, F. Abinzano, M. A. Tryfonidou, S. J. P. Callens, K. Ito
M‐MLV Reverse Transcriptase Kit (Invitrogen, #10338842, Waltham, MA, USA) and random primers (
Promega
, #
C1181
, Madison, WI, USA).
RT‐qPCR
was performed using SYBR Green Master Mix (Bio‐Rad, #170‐8887, Hercules,
(4)
Seedless 3' pairing enables miR-17 family miRNAs to seize let-7 target sites
Nucleic Acids Res
August 14, 2026
Artur Laski, Anneke Brümmer, Matije Lucic, Elisa Federici, Iris Mestres-Pascual, Jochen Imig, Alexander Kanitz, Mihaela Zavolan, Luca F R Gebert, Ian J MacRae,
et al.
TaqMan MicroRNA RT Kit (
Thermo Fisher Scientific
, #
4366596
) with a
1:1
mix of random hexamer (
Promega
, #
C1181
) and oligo (dT)15 primers (
Promega
, #
C1101
).
qPCR
was performed using KAPA SYBR
FAST qPCR
master mix
(5)
Postprandial dynamics of sensory receptor and neural mechanisms underlying feeding regulation in mule ducks
Poultry Science
August 1, 2026
Marie Lasserre, Yan Fericelli, Cécile Heraud, Laura-Lou Zwick, Karel Sasso, Sandra Biasutti, Anne Surget, Marianne Houssier, Stéphane Panserat, Jérôme Roy,
et al.
SuperScript III Reverse Transcriptase
(
Invitrogen
;
18080044
),
Random Primers
(
Promega
, Madison, WI, USA;
C1181
), and
PCR nucleotide mix
(
Promega
,
C1145
). Negative controls (no-RT and no-template) were included to
(6)
Identification of Enhancers and Transcription Factors Regulating Postnatal Growth of Skeletal Muscle in Cattle
Biology (Basel)
July 29, 2026
Pengcheng Lyu, Binod Pokhrel, Jianguo Zhao, Honglin Jiang
[26,27]. 2.5.
RT-qPCR
and Data Analysis Reverse transcription was performed using random primers (catalog #
C1181
,
Promega
, Madison, WI, USA), IImProm-IIReverse Transcriptase (catalog #
A3803
,
Promega
, Madison, WI,
(7)
Retargeted adenoviruses for local IgA and CD47 blocker production as a novel cancer therapy
EMBO Molecular Medicine
July 7, 2026
Mariya Chernyavska, K Patricia Hartmann, J H Marco Jansen, Niklas Baumann, Jonas Kolibius, Dominik Brücher, Theodora Kristoforus, Rens H W Peters, Lucas Huijs, Daphne Laarveld,
et al.
Scientific), and 1000 ng of RNA was used for
cDNA synthesis
. After
incubation
with random primers (#
C1181
,
Promega
) and deoxynucleotides (#
N0447L
,
New England BioLabs
) at 65 °C for 5 min, a mix of RNAse inhibitor
(8)
Protocol for massively parallel RNA assay combined with immunoprecipitation for high-throughput analysis of RNA-protein interactions in cells
STAR Protoc
June 19, 2026
Yu Hsuan Lee, John L. Rinn, Taeyoung Hwang
,
Thermo Scientific
,
R0861
Proteinase K,
Invitrogen
,
AM2546
RNaseOUT,
Invitrogen
,
10777019
Random Primers
,
Promega
,
C1181
"PBS (10X), pH 7.4",Quality Biological,119-069-131 "
PBS, pH 7.4
",
Gibco
,
10010023
"Tris-HCl (1 M),
(9)
Protocol for quantifying silent ribosome induction in yeast and mammalian cells using polysome profiling
STAR Protocols
June 19, 2026
Sayanur Rahaman, Samuel Mondal, Nicole Schiffelholz, Attila Becskei
Scientific,Cat#18090200 Nuclease Free Water,
Thermo Scientific
,Cat#
AM9932
Random hexamers,
Promega
,Cat#
C1181
oligodT15,
Promega
,Cat#
C1101
KAPA SYBR Fast,Sigma Aldrich,Cat#KK4611 Tris-HCl pH 8.0,
Thermo Scientific
,Cat#
AM9856
MgCl2,
Thermo Scientific
(10)
Activation of AMPK as a therapeutic strategy for FBXL4-related mitochondrial DNA depletion syndrome
EMBO Molecular Medicine
June 17, 2026
Aniketh Bishnu, Juan Zapata-Muñoz, Robert W Taylor, Kei Sakamoto, Ian G Ganley
manufacturer’s instructions. About 1 μg of RNA was then converted to CDNA using
Random primers
(
Promega
#
C1181
) using the
SuperScript III reverse transcriptase
(
Thermo Fisher
#
18080044
) following the manufacturer’s
(11)
Short- and Long-Term Chrono-Immune Consequences of Dim Light at Night Exposure in Male Mice at Different Life Stages
Clocks Sleep
June 17, 2026
Carlos A. Trujillo, Fernando Miranda, José Sarmiento
manufacturer’s instructions. First-strand cDNA was synthesized using random primers (
Promega
® Cat. #
C1181
, Madison, WI, USA), a
dNTP mix
(dATP, dCTP, dGTP, dTTP
Promega
® Cat. #
U1330
, Madison, WI, USA), and
(12)
Multi-omic screening identifies RBMXL3 as a primate-specific RNA-binding protein and candidate regulator of RNA metabolism in human spermatogenesis
Cellular & Molecular Biology Letters
May 27, 2026
Agata J. Barszcz, Katarzyna Tutak, Joanna Zyprych-Walczak, Erik Dassi, Erkut Ilaslan, Małgorzata Dąbrowska, Agnieszka Malcher, Marta Olszewska, Dominik Cysewski, Agnieszka Nadel,
et al.
SuperScript IV Reverse Transcriptase
kit (
Thermo Fisher Scientific
,
18090050
) with the use of random primers (
Promega
,
C1181
). For quantitative polymerase chain reaction (qPCR) 10 ng of complementary cDNA was mixed with
PowerUp SYBR Green Master Mix
(13)
The RNA helicase DDX53 (CAGE) contributes to RNA metabolism in a human germ cell model
bioRxiv
May 25, 2026
Agata J. Barszcz, Katarzyna Tutak, Agnieszka Malcher, Małgorzata Dąbrowska, Joanna Zyprych-Walczak, Erik Dassi, Erkut Ilaslan, Marta Olszewska, Dominik Cysewski, Michał Hrab,
et al.
SuperScript IV Reverse Transcriptase
kit (
Thermo Fisher Scientific
,
18090050
) with the use of random primers (
Promega
,
C1181
) using 1 μg of total RNA for each reaction. Ten ng of cDNA was used for
qPCR
, performed with
PowerUp SYBR Green Master Mix
(14)
Macrophage-mediated refinement of the dural lymphatic regulates social behavior
Neuron
April 24, 2026
Gabriel A. Tavares, Liam O'Reilly, Natalie M. Frederick, David A. Shapiro, Ashleigh Vicchiarelli, Megan Sullivan, Nathan Jiang, Maximillian Mattes, Coby Goelz, Nnadozie Uchegbu,
et al.
dNTP (10mM) (
Promega
, cat.
U1515
), Oligo DT (
Promega
, cat.
C1101
), and random primers (
Promega
, cat.
C1181
). The reverse transcription was performed with the
SuperScript™ III Reverse Transcriptase
kit (
Invitrogen
™,
(15)
Lymphotoxin-beta receptor controls the development of chronic pain
Cell reports
April 20, 2026
Tumanov AV, Mecklenburg JM, Belugin S, Naratadam GT, Barba-Escobedo PA, Todd AW, Koroleva E, Hovhannisyan AH, Shein SA, Tamada K,
et al
.
Superscript II reverse transcriptase
,
Invitrogen
,Cat# 18064-014 10mM dNTP mix,
Invitrogen
,Cat#
18427013
Random Primers
,
Promega
,Cat#
C1181
Deposited data,Deposited data,Deposited data Bulk RNAseq,This paper GEO,GSE302112; GSE302021; GSE302020;
(16)
Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxin
Infection and immunity
April 15, 2026
Schaaf D, Dresen M, Weldearegay YB, Biermann J, Brockmeier SL, Baumgärtner W, Jarek M, Valentin-Weigand P.
M3682
), dNTP Mix (10 mM each; Carl Roth, Cat. No. K039.1) and 500 ng
Random Primers
(
Promega
, Cat. No.
C1181
). For no-RT controls, the reverse transcriptase was replaced by
RNase-free water
. NoRT- and cDNA samples
(17)
Regulation of murine follicle-stimulating hormone β subunit transcription by newly identified enhancers
Endocrinology
April 7, 2026
Yangfan Jin, Hailey Schultz, Luisina Ongaro, Gauthier Schang, Xiang Zhou, Carlos Agustin Isidro Alonso, Michel Zamojski, German Nudelman, Natalia Mendelev, Shinsuke Onuma,
et al.
RNA were treated with RQ1 DNase and then reverse-transcribed into cDNA using random hexamer primers (
C1181
,
Promega
) and
Moloney murine leukemia virus
reverse transcriptase (
M1701
,
Promega
). Each reverse transcription
(18)
A regulatory circuit operated by KDELR1 and KDELR3 fine-tunes the composition of the early secretory pathway
Cellular and Molecular Life Sciences
February 18, 2026
Federica Cecilia Palazzo, Marco Dalla Torre, Yuta Amagai, Xue Han, Tiziana Tempio, Caterina Valetti, Jose Garcia Manteiga, Masaki Matsumoto, Matthias Feige, Michele Sallese,
et al.
to cDNA was performed with
M-MLV Reverse Transcriptase
and random primers (
Promega
, Cat#
M1701
; Cat#
C1181
) following the manufacturer’s protocol for 2 μg RNA. Real-time semi-
quantitative PCR
was performed with
(19)
MYC modulates TOP2A diffusion to promote substrate detection and activity
Nature Communications
February 9, 2026
Donald P. Cameron, Kathryn Jackson, Alessia Loffreda, Carl Möller, Vladislav Kuzin, Matteo Mazzocca, Evanthia Iliopoulou, Hallgerdur Kolbeinsdottir, Andrej Paluda, Evgeniya Pavlova,
et al.
dATP, dCTP, dGTP, dTTP;
Promega
,
U1205
,
U1215
,
U1225
,
U1235
) and 0.5 ul of random hexamers (
Promega
,
C1181
). This mixture was heated at 65 °C for 5 min, then put on ice for 1 min. 4 ul RT buffer, 0.3 ul
SuperScript IV Reverse Transcriptase
(20)
Combinations of Favipiravir with Doxycycline, Azithromycin or Ivermectin Exert Synergistic Effects Against Influenza A H3N2 Virus Replication
Pathogens
February 4, 2026
Kuan Chien Tan, Julia H. Y. Neo, Thai Tran, Vincent T. K. Chow
(150 ng) was topped up to 6.25 μL with nuclease-free water, and 0.5 μL of random primers (
Promega
,
C1181
) was added. The mixture was first placed in the thermocycler at 70 °C for 5 min and then placed in ice
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