Random Primers from Promega

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ItemRandom Primers
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC1181
TargetRandom
Quantity20microg
Primer TypeRandom Primers

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Macrophage-mediated refinement of the dural lymphatic regulates social behaviorNeuronApril 24, 2026Gabriel A. Tavares, Liam O'Reilly, Natalie M. Frederick, David A. Shapiro, Ashleigh Vicchiarelli, Megan Sullivan, Nathan Jiang, Maximillian Mattes, Coby Goelz, Nnadozie Uchegbu, et al.dNTP (10mM) (Promega, cat. U1515), Oligo DT (Promega, cat. C1101), and random primers (Promega, cat. C1181). The reverse transcription was performed with the SuperScript™ III Reverse Transcriptase kit (Invitrogen™,

(2) Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxinInfection and ImmunityApril 15, 2026Désirée Schaaf, Muriel Dresen, Yenehiwot Berhanu Weldearegay, Jeannine Biermann, Susan L. Brockmeier, Wolfgang Baumgärtner, Michael Jarek, Peter Valentin-WeigandM3682), dNTP Mix (10 mM each; Carl Roth, Cat. No. K039.1) and 500 ng Random Primers (Promega, Cat. No. C1181). For no-RT controls, the reverse transcriptase was replaced by RNase-free water. NoRT- and cDNA samples

(3) Regulation of murine follicle-stimulating hormone β subunit transcription by newly identified enhancersEndocrinologyApril 7, 2026Yangfan Jin, Hailey Schultz, Luisina Ongaro, Gauthier Schang, Xiang Zhou, Carlos Agustin Isidro Alonso, Michel Zamojski, German Nudelman, Natalia Mendelev, Shinsuke Onuma, et al.RNA were treated with RQ1 DNase and then reverse-transcribed into cDNA using random hexamer primers (C1181, Promega) and Moloney murine leukemia virus reverse transcriptase (M1701, Promega). Each reverse transcription

(4) The Intracellular C5a–mtC5aR1 Axis Promotes Necroptosis in Dry Eye Through DRP1-Mediated Mitochondrial DysfunctionInvestigative Ophthalmology & Visual ScienceFebruary 18, 2026Limei Wang, Haijing Yan, Yetao Shen, Songlin Xin, Chenyu Dong, Jiuxiao Li, Yueping Ren, Wei Chen, Qinxiang Zhengµg total RNA was used for reverse transcription into cDNA using a random primer (70°C, 5 minutes) (C1181; Promega, Madison, WI, USA), M-MLV reverse transcriptase (M1701; Promega), and Recombinant RNasin Ribonuclease Inhibitor

(5) A regulatory circuit operated by KDELR1 and KDELR3 fine-tunes the composition of the early secretory pathwayCellular and Molecular Life SciencesFebruary 18, 2026Federica Cecilia Palazzo, Marco Dalla Torre, Yuta Amagai, Xue Han, Tiziana Tempio, Caterina Valetti, Jose Garcia Manteiga, Masaki Matsumoto, Matthias Feige, Michele Sallese, et al.to cDNA was performed with M-MLV Reverse Transcriptase and random primers (Promega, Cat#M1701; Cat#C1181) following the manufacturer’s protocol for 2 μg RNA. Real-time semi-quantitative PCR was performed with

(6) MYC modulates TOP2A diffusion to promote substrate detection and activityNature CommunicationsFebruary 9, 2026Donald P. Cameron, Kathryn Jackson, Alessia Loffreda, Carl Möller, Vladislav Kuzin, Matteo Mazzocca, Evanthia Iliopoulou, Hallgerdur Kolbeinsdottir, Andrej Paluda, Evgeniya Pavlova, et al.dATP, dCTP, dGTP, dTTP; Promega, U1205, U1215, U1225, U1235) and 0.5 ul of random hexamers (Promega, C1181). This mixture was heated at 65 °C for 5 min, then put on ice for 1 min. 4 ul RT buffer, 0.3 ul SuperScript IV Reverse Transcriptase

(7) Combinations of Favipiravir with Doxycycline, Azithromycin or Ivermectin Exert Synergistic Effects Against Influenza A H3N2 Virus ReplicationPathogensFebruary 4, 2026Kuan Chien Tan, Julia H. Y. Neo, Thai Tran, Vincent T. K. Chow(150 ng) was topped up to 6.25 μL with nuclease-free water, and 0.5 μL of random primers (Promega, C1181) was added. The mixture was first placed in the thermocycler at 70 °C for 5 min and then placed in ice

(8) Human Liver Organoids as an Experimental Tool to Investigate Lipocalin-2 in Hepatic InflammationCellsJanuary 23, 2026Katharina S. Hardt, Robert F. Pohlberger, Diandra T. Keller, Eva M. Buhl, Florian W. R. Vondran, Anjali A. Roeth, Ralf Weiskirchen, Sarah K. Schröder-LangeSuperscript II reverse transcriptase (18064022, Invitrogen, Thermo Fisher Scientific) and random primers (C1181, Promega, Madison, WI, USA). For RT-qPCR, 25 ng of cDNA and 20 pmol of forward and reverse primers diluted

(9) Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cellsCommunications BiologyJanuary 19, 2026Jorik Frederik Bot, Zhihan Zhao, Mengyuan Li, Darnell Kammeron, Peng Shang, Niels Geijsenmin. The NEBNext second strand synthesis kit (NEB, E6111) with 100 ng random hexamer primers (Promega, C1181) per sample was used for second strand synthesis. Next, dsDNA was purified using AMPure XP beads (Beckman Coulter

(10) Long non-coding RNA UCA1 modulates SMARCA2-containing SWI/SNF chromatin remodeling complexes in human colorectal canceriScienceJanuary 16, 2026Bernadette Neve, Elsa Hadj Bachir, Belinda Duchêne, Mouloud Souidi, Martin Figeac, Jean-Pascal Meneboo, Emmanuelle Leteurtre, Christophe Mariette, Guillaume Piessen, François Corfiotti, et al.recombinant proteins" ,, M-MLV reverse transcriptase,Promega,Cat#M1705 random hexamer primers,Promega,Cat#C1181 DMEM,Thermo Fisher Scientific,Cat#41965-039 Fetal Bovine Serum (FBS),Thermo Fisher Scientific,Cat#10270106 Gibco™

(11) Spatial heterogeneity of lactylation: insights into gene expression, metabolism, and lactate transport in human embryonic stem cellsBiology OpenJanuary 1, 2026Alexandra M. Kozlov, Zuleika C. L. Leung, Rachel B. Wilson, Sukhdeep Bhangal, Karen Nygard, Andrew M. Powell, Nica M. Borradaile, Dean H. Betts, Robert C. CummingMoloney's-Murine Leukemia Virus (M-MLV) Reverse Transcriptase (#28025-021; Invitrogen), Random Primers (#C1181; Promega), 10 mM Nucleotide-dNTP's (#R0181; Thermo Fisher Scientific), and HyPure™ Molecular Biology

(12) Refeeding with different levels of dietary carbohydrate modulates epigenetic stability through global DNA (de)methylation and histone modifications in juvenile and adult Nile tilapia ( Oreochromis niloticus )EpigeneticsDecember 31, 2025Sirijanya Thongchaitriwat, Suksan Kumkhong, Cécile Heraud, Karine Dias, Stephane Panserat, Surintorn Boonanuntanasarn, Lucie Marandel10777019), dNTP mix (Promega, France; cat. no. U1511), and random primers (Promega, France; cat. no. C1181), following the manufacturer’s protocol. Reverse transcription (duplicate for each sample) was performed,

(13) Humanizing Mice for Human Immunodeficiency Virus Infection StudiesCurrent ProtocolsDecember 29, 2025Chandra Nath Roy, Christopher Kline, Ashley Gnanasekaran, Zandrea Ambrose10297018) 100 mM USB DTT (Invitrogen, cat. no. 707265ML) 500 μg/ml random hexamers, (Promega, cat. no. C1181) 40 U/μl RNasin ribonuclease inhibitor (Promega, cat. no. N2515) 200 U/μl M-MLV reverse transcriptase

(14) Shaping 3D minimal model tissues with mechanical constraints to orchestrate muscle differentiationCommunications BiologyNovember 28, 2025Irène Nagle, Lorijn van der Spek, Paul Gesenhues, Thierry Savy, Laurent Réa, Alain Richert, Mathieu Receveur, Florence Delort, Sabrina Batonnet-Pichon, Claire Wilhelm, et al.SuperScript II Reverse Transcriptase kit (18064022, Thermofischer Scientific) and random hexamers (C1181, Promega) according to the manufacturer’s instructions. Quantitative PCR was performed with SYBR Green

(15) Restoration of BAP1 activity via base editing suppresses anchorage-independent survival in kidney cancerCancer Cell InternationalNovember 25, 2025Daye Lee, Boram Lee, Jiyeon Lee, Jongbum Kwonusing HelixCript thermo reverse transcriptase (Nanohelix, RT50K) and random hexamer primers (Promega, C1181). The resulting cDNA was used as a template for PCR amplification, and the PCR products were resolved

(16) MicroRNA response in insect salivary glands to plant virus infectionJournal of VirologyNovember 25, 2025Yan Xiao, Guohua Liang, Jiaming Zhu, Feng Cui, Wan ZhaoSuperScript III First-Strand Synthesis System (Thermo Scientific; 18080051) with random hexamer primers (Promega, Madison, WI; C1181). qPCR was performed on a LightCycler 480 Instrument II (Roche, Basel, Switzerland). miRNA expression

(17) Regulation of the mechanoresponsive Neat1 and PSPC1 by substrate stiffness in TGF-β1-induced renal progenitor cell fateJournal of Biomedical ScienceNovember 17, 2025Hsiao-Ning Huang, Lun-Wei Lee, Cheng-Hsiang Kuo, Tzyy Yue Wong, Wen-Tai Chiu, Ming-Jer TangSuperScript IV reverse transcriptase (Life Technologies, Cat. No.18090050) and random hexamer primers (Promega, Cat. No. C1181). qRT-PCR was conducted in triplicate on a StepOne™ real-time PCR instrument, and the data were analyzed

(18) Wnt signalling maintains self-renewal of human hepatoblasts without blocking their differentiationDevelopmentNovember 15, 2025Ekaterini D. Zacharis, Carola M. Morell, Rute A. Tomaz, Arash Shahsavari, Charlotte Grey-Wilson, Maïa Pesic, Vasileios Galanakis, Eleanor C. Williams, Irina Mohorianu, Irene Talon, et al.transcription Five-hundred nanograms of total RNA was added to 250 µg/µl of random primers (Promega, C1181) and 0.5 mM dNTPs (Promega, U1511). This mixture was heated to 65°C for 5 min and immediately transferred

(19) Jpx RNA controls Xist induction through spatial reorganization of the mouse X-inactivation centerDevelopmental CellNovember 3, 2025Hyun Jung Oh, Priyojit Das, Roy Blum, Andrea J. Kriz, Hun-Goo Lee, Yong-Woo Lee, Jeannie T. Lee(Thermo Fisher Scientific, AM1907), the resulting RNA was subjected to cDNA synthesis with random primers (Promega, C1181) using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific, 18080085). RNA expression was

(20) Differential stress responses of immunoisolated human islets embedded in pancreatic extracellular matrix under static and free-fall dynamic conditionsJ Tissue EngOctober 27, 2025Isaura Borges-Silva, Marluce da Cunha Mantovani, Minh Danh Anh Luu, Alan Gorter, Theo Borghuis, Naschla Gasaly, Mari Cleide Sogayar, Paul deVos, Marina Trombetta-Limadeoxynucleoside triphosphate (dNTP; Promega, cat# U1511) and 0.5 μg/μL random primers (Promega, Netherlands cat#C1181) were added into the RNA sample and heated at 70°C for 5 min using MJ Research PTC-200 Thermal Cycler

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Random Primers

Random Primers from Promega

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Random Primers from Promega

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