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Polymerase Chain Reaction (PCR)
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Random Primers
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Random Primers
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Random Primers from Promega
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Citations:
(237)
Figures:
(1)
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Description
Use for first-strand cDNA synthesis with a reverse transcriptase
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Random Primers
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
C1181
Target
Random
Quantity
20microg
Primer Type
Random Primers
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (237)
(1)
Embryonic thermal programming combined with fractionated feeding induces early activation of hepatic lipid storage pathways in mule ducks
Poultry Science
September 1, 2026
Laura-Lou Zwick, Joséphine Huot, Cécile Heraud, Marie Lasserre, Anne Surget, Karine Gontier, Jérôme Roy, Stéphane Panserat, Marianne Houssier
µl RNase-free water. The reaction included
Random Primers
(
Promega
, Madison, WI; product reference:
C1181
, 0.2 µg/ml final), a
PCR
nucleotides mix (
Promega
, Madison, WI; product reference:
C1145
, 0.004 mM final),
(2)
Postprandial dynamics of sensory receptor and neural mechanisms underlying feeding regulation in mule ducks
Poultry Science
August 1, 2026
Marie Lasserre, Yan Fericelli, Cécile Heraud, Laura-Lou Zwick, Karel Sasso, Sandra Biasutti, Anne Surget, Marianne Houssier, Stéphane Panserat, Jérôme Roy,
et al.
SuperScript III Reverse Transcriptase
(
Invitrogen
;
18080044
),
Random Primers
(
Promega
, Madison, WI, USA;
C1181
), and
PCR nucleotide mix
(
Promega
,
C1145
). Negative controls (no-RT and no-template) were included to
(3)
Protocol for quantifying silent ribosome induction in yeast and mammalian cells using polysome profiling
STAR Protocols
June 19, 2026
Sayanur Rahaman, Samuel Mondal, Nicole Schiffelholz, Attila Becskei
Scientific,Cat#18090200 Nuclease Free Water,
Thermo Scientific
,Cat#
AM9932
Random hexamers,
Promega
,Cat#
C1181
oligodT15,
Promega
,Cat#
C1101
KAPA SYBR Fast,Sigma Aldrich,Cat#KK4611 Tris-HCl pH 8.0,
Thermo Scientific
,Cat#
AM9856
MgCl2,
Thermo Scientific
(4)
Protocol for massively parallel RNA assay combined with immunoprecipitation for high-throughput analysis of RNA-protein interactions in cells
STAR Protoc
June 19, 2026
Yu Hsuan Lee, John L. Rinn, Taeyoung Hwang
,
Thermo Scientific
,
R0861
Proteinase K,
Invitrogen
,
AM2546
RNaseOUT,
Invitrogen
,
10777019
Random Primers
,
Promega
,
C1181
"PBS (10X), pH 7.4",Quality Biological,119-069-131 "
PBS, pH 7.4
",
Gibco
,
10010023
"Tris-HCl (1 M),
(5)
Short- and Long-Term Chrono-Immune Consequences of Dim Light at Night Exposure in Male Mice at Different Life Stages
Clocks Sleep
June 17, 2026
Carlos A. Trujillo, Fernando Miranda, José Sarmiento
manufacturer’s instructions. First-strand cDNA was synthesized using random primers (
Promega
® Cat. #
C1181
, Madison, WI, USA), a
dNTP mix
(dATP, dCTP, dGTP, dTTP
Promega
® Cat. #
U1330
, Madison, WI, USA), and
(6)
The RNA helicase DDX53 (CAGE) contributes to RNA metabolism in a human germ cell model
bioRxiv
May 25, 2026
Agata J. Barszcz, Katarzyna Tutak, Agnieszka Malcher, Małgorzata Dąbrowska, Joanna Zyprych-Walczak, Erik Dassi, Erkut Ilaslan, Marta Olszewska, Dominik Cysewski, Michał Hrab,
et al.
SuperScript IV Reverse Transcriptase
kit (
Thermo Fisher Scientific
,
18090050
) with the use of random primers (
Promega
,
C1181
) using 1 μg of total RNA for each reaction. Ten ng of cDNA was used for
qPCR
, performed with
PowerUp SYBR Green Master Mix
(7)
Macrophage-mediated refinement of the dural lymphatic regulates social behavior
Neuron
April 24, 2026
Gabriel A. Tavares, Liam O'Reilly, Natalie M. Frederick, David A. Shapiro, Ashleigh Vicchiarelli, Megan Sullivan, Nathan Jiang, Maximillian Mattes, Coby Goelz, Nnadozie Uchegbu,
et al.
dNTP (10mM) (
Promega
, cat.
U1515
), Oligo DT (
Promega
, cat.
C1101
), and random primers (
Promega
, cat.
C1181
). The reverse transcription was performed with the
SuperScript™ III Reverse Transcriptase
kit (
Invitrogen
™,
(8)
Lymphotoxin-beta receptor controls the development of chronic pain
Cell Reports
April 20, 2026
Alexei V. Tumanov, Jennifer M. Mecklenburg, Sergei Belugin, George T. Naratadam, Priscilla A. Barba-Escobedo, Austin W. Todd, Ekaterina Koroleva, Anahit H. Hovhannisyan, Sergey A. Shein, Koji Tamada,
et al.
Superscript II reverse transcriptase
,
Invitrogen
,Cat# 18064-014 10mM dNTP mix,
Invitrogen
,Cat#
18427013
Random Primers
,
Promega
,Cat#
C1181
Deposited data,Deposited data,Deposited data Bulk RNAseq,This paper GEO,GSE302112; GSE302021; GSE302020;
(9)
Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxin
Infection and immunity
April 15, 2026
Schaaf D, Dresen M, Weldearegay YB, Biermann J, Brockmeier SL, Baumgärtner W, Jarek M, Valentin-Weigand P.
M3682
), dNTP Mix (10 mM each; Carl Roth, Cat. No. K039.1) and 500 ng
Random Primers
(
Promega
, Cat. No.
C1181
). For no-RT controls, the reverse transcriptase was replaced by
RNase-free water
. NoRT- and cDNA samples
(10)
Regulation of murine follicle-stimulating hormone β subunit transcription by newly identified enhancers
Endocrinology
April 7, 2026
Yangfan Jin, Hailey Schultz, Luisina Ongaro, Gauthier Schang, Xiang Zhou, Carlos Agustin Isidro Alonso, Michel Zamojski, German Nudelman, Natalia Mendelev, Shinsuke Onuma,
et al.
RNA were treated with RQ1 DNase and then reverse-transcribed into cDNA using random hexamer primers (
C1181
,
Promega
) and
Moloney murine leukemia virus
reverse transcriptase (
M1701
,
Promega
). Each reverse transcription
(11)
A regulatory circuit operated by KDELR1 and KDELR3 fine-tunes the composition of the early secretory pathway
Cellular and Molecular Life Sciences
February 18, 2026
Federica Cecilia Palazzo, Marco Dalla Torre, Yuta Amagai, Xue Han, Tiziana Tempio, Caterina Valetti, Jose Garcia Manteiga, Masaki Matsumoto, Matthias Feige, Michele Sallese,
et al.
to cDNA was performed with
M-MLV Reverse Transcriptase
and random primers (
Promega
, Cat#
M1701
; Cat#
C1181
) following the manufacturer’s protocol for 2 μg RNA. Real-time semi-
quantitative PCR
was performed with
(12)
The Intracellular C5a–mtC5aR1 Axis Promotes Necroptosis in Dry Eye Through DRP1-Mediated Mitochondrial Dysfunction
Investigative Ophthalmology & Visual Science
February 18, 2026
Limei Wang, Haijing Yan, Yetao Shen, Songlin Xin, Chenyu Dong, Jiuxiao Li, Yueping Ren, Wei Chen, Qinxiang Zheng
µg total RNA was used for reverse transcription into cDNA using a random primer (70°C, 5 minutes) (
C1181
;
Promega
, Madison, WI, USA),
M-MLV reverse transcriptase
(
M1701
;
Promega
), and
Recombinant RNasin Ribonuclease Inhibitor
(13)
MYC modulates TOP2A diffusion to promote substrate detection and activity
Nature Communications
February 9, 2026
Donald P. Cameron, Kathryn Jackson, Alessia Loffreda, Carl Möller, Vladislav Kuzin, Matteo Mazzocca, Evanthia Iliopoulou, Hallgerdur Kolbeinsdottir, Andrej Paluda, Evgeniya Pavlova,
et al.
dATP, dCTP, dGTP, dTTP;
Promega
,
U1205
,
U1215
,
U1225
,
U1235
) and 0.5 ul of random hexamers (
Promega
,
C1181
). This mixture was heated at 65 °C for 5 min, then put on ice for 1 min. 4 ul RT buffer, 0.3 ul
SuperScript IV Reverse Transcriptase
(14)
Combinations of Favipiravir with Doxycycline, Azithromycin or Ivermectin Exert Synergistic Effects Against Influenza A H3N2 Virus Replication
Pathogens
February 4, 2026
Kuan Chien Tan, Julia H. Y. Neo, Thai Tran, Vincent T. K. Chow
(150 ng) was topped up to 6.25 μL with nuclease-free water, and 0.5 μL of random primers (
Promega
,
C1181
) was added. The mixture was first placed in the thermocycler at 70 °C for 5 min and then placed in ice
(15)
Human Liver Organoids as an Experimental Tool to Investigate Lipocalin-2 in Hepatic Inflammation
Cells
January 23, 2026
Hardt KS, Pohlberger RF, Keller DT, Buhl EM, Vondran FWR, Roeth AA, Weiskirchen R, Schröder-Lange SK.
Superscript II reverse transcriptase
(
18064022
,
Invitrogen
, Thermo Fisher Scientific) and random primers (
C1181
,
Promega
, Madison, WI, USA). For
RT-qPCR
, 25 ng of cDNA and 20 pmol of forward and reverse primers diluted
(16)
Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells
Communications Biology
January 19, 2026
Jorik Frederik Bot, Zhihan Zhao, Mengyuan Li, Darnell Kammeron, Peng Shang, Niels Geijsen
min. The NEBNext second strand synthesis kit (
NEB
,
E6111
) with 100 ng random hexamer primers (
Promega
,
C1181
) per sample was used for second strand synthesis. Next, dsDNA was purified using AMPure XP beads (
Beckman Coulter
(17)
Long non-coding RNA UCA1 modulates SMARCA2-containing SWI/SNF chromatin remodeling complexes in human colorectal cancer
iScience
January 16, 2026
Bernadette Neve, Elsa Hadj Bachir, Belinda Duchêne, Mouloud Souidi, Martin Figeac, Jean-Pascal Meneboo, Emmanuelle Leteurtre, Christophe Mariette, Guillaume Piessen, François Corfiotti,
et al.
recombinant proteins" ,,
M-MLV reverse transcriptase
,
Promega
,Cat#
M1705
random hexamer primers,
Promega
,Cat#
C1181
DMEM,Thermo Fisher Scientific,Cat#41965-039 Fetal Bovine Serum (FBS),Thermo Fisher Scientific,Cat#10270106 Gibco™
(18)
Spatial heterogeneity of lactylation: insights into gene expression, metabolism, and lactate transport in human embryonic stem cells
Biology Open
January 1, 2026
Alexandra M. Kozlov, Zuleika C. L. Leung, Rachel B. Wilson, Sukhdeep Bhangal, Karen Nygard, Andrew M. Powell, Nica M. Borradaile, Dean H. Betts, Robert C. Cumming
Moloney's-
Murine Leukemia Virus
(M-MLV) Reverse Transcriptase (#28025-021;
Invitrogen
),
Random Primers
(#
C1181
;
Promega
), 10 mM Nucleotide-dNTP's (#
R0181
;
Thermo Fisher Scientific
), and HyPure™ Molecular Biology
(19)
Refeeding with different levels of dietary carbohydrate modulates epigenetic stability through global DNA (de)methylation and histone modifications in juvenile and adult Nile tilapia ( Oreochromis niloticus )
Epigenetics
December 31, 2025
Sirijanya Thongchaitriwat, Suksan Kumkhong, Cécile Heraud, Karine Dias, Stephane Panserat, Surintorn Boonanuntanasarn, Lucie Marandel
10777019
),
dNTP mix
(
Promega
, France; cat. no.
U1511
), and random primers (
Promega
, France; cat. no.
C1181
), following the manufacturer’s protocol. Reverse transcription (duplicate for each sample) was performed,
(20)
Shaping 3D minimal model tissues with mechanical constraints to orchestrate muscle differentiation
Communications Biology
November 28, 2025
Irène Nagle, Lorijn van der Spek, Paul Gesenhues, Thierry Savy, Laurent Réa, Alain Richert, Mathieu Receveur, Florence Delort, Sabrina Batonnet-Pichon, Claire Wilhelm,
et al.
SuperScript II Reverse Transcriptase
kit (
18064022
,
Thermofischer
Scientific) and random hexamers (
C1181
,
Promega
) according to the manufacturer’s instructions.
Quantitative PCR
was performed with SYBR Green
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