Description
A fluorescently tagged cholesterol with the hydrophilic NBD fluorophore attached to carbon 3, at the hydrophilic end of cholesterol, separated by a 6-carbon spacer; allows the cholesterol to properly orient in membrane bi-layers while the fluorescent tag is presented outside of the bi-layer; ex/em = 473/536 nm in vesicles comprised of DOPC but will vary depending on membrane composition