Nuclear & Cytoplasmic Extraction Kit from G Biosciences

Product Specs
ItemNuclear & Cytoplasmic Extraction Kit
CompanyG Biosciences
Price $216.00Supplier Page View Company Product Page
Catalog Number786-182
Quantity100 Preps
ApplicationsExtraction of cytosolic and nuclear protein fractions for transport studies, electrophoresis and other applications

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G Biosciences

9800 Page Avenue

St. Louis, Missouri 63132

United States

Phone: +800-628-7730
Fax: 314-991-1504

Company Profile

Website: http://www.gbiosciences.com

(1) An N‐terminal and ankyrin repeat domain interactome of Shank3 identifies the protein complex with the splicing regulator Nono in miceGenes to CellsSeptember 1, 2024Sayaka Okuzono, Fumihiko Fujii, Daiki Setoyama, Ryoji Taira, Yohei Shinmyo, Hiroki Kato, Keiji Masuda, Kousuke Yonemoto, Satoshi Akamine, Yuki Matsushita, et al.cytosolic fractions were prepared using the MinuteTM Cytoplasmic and Nuclear Extraction Kit for Cells (786–182; G‐Bioscience, St. Louis, MO, USA). In brief, logarithmically growing Neuro2a cells (1 × 105 cells)

(2) Focal Adhesion Kinase (FAK)-Hippo/YAP transduction signaling mediates the stimulatory effects exerted by S100A8/A9-RAGE system in triple-negative breast cancer (TNBC)Journal of experimental & clinical cancer research : CRJune 3, 2022Rigiracciolo DC, Nohata N, Lappano R, Cirillo F, Talia M, Adame-Garcia SR, Arang N, Lubrano S, De Francesco EM, Belfiore A, et al.subcellular fractionated lysates was performed using The Nuclear & Cytoplasmic Extraction Kit (Cat. #786–182, G-Biosciences) following the manufacturer instructions. Briefly, after treatments, MDA-MB231 TNBC cells

(3) (+)-JJ-74-138 is a Novel Noncompetitive Androgen Receptor AntagonistMolecular cancer therapeuticsApril 1, 2022Cole RN, Chen W, Pascal LE, Nelson JB, Wipf P, Wang Z.PMSF. Nucleocytoplasmic fractionation was performed according to the manufacturers (G-Biosciences, 786–182) protocol. Protein extracts were boiled in SDS sample buffer and bands were separated by size by SDS-PAGE.

(4) β-actin dependent chromatin remodeling mediates compartment level changes in 3D genome architectureNature CommunicationsDecember 1, 2021Syed Raza Mahmood, Xin Xie, Nadine Hosny El Said, Tomas Venit, Kristin C. Gunsalus, Piergiorgio Percipallecollected for nuclear and cytoplasmic fraction using Nuclear & Cytoplasmic Extraction Kit (G-Bioscience, 786-182, St. Louis, USA). Briefly, cells were trypsinized and counted by hemocytometer and the collected cell

(5) In vitro effects of resistin on epithelial to mesenchymal transition (EMT) in MCF-7 and MDA-MB-231 breast cancer cells - qRT-PCR and Westen blot analyses dataData in briefAugust 1, 2019Avtanski D, Garcia A, Caraballo B, Thangeswaran P, Marin S, Bianco J, Lavi A, Poretsky L.protein fractures were extracted by using Nuclear & Cytoplasmic Extraction Kit (G Biosciences®, Cat. # 786-182) following manufacturer’s protocol. Protein concentrations were quantified by using Pierce™ BCA Protein Assay Kit

(6) Renal Damaging Effect Elicited by Bicalutamide Therapy Uncovered Multiple Action Mechanisms As Evidenced by the Cell ModelScientific reportsMarch 4, 2019Peng CC, Chen CY, Chen CR, Chen CJ, Shen KH, Chen KC, Peng RY.were used for extraction of nuclear proteins according to the manufacturer’s instructions (cat. no. 786–182., G-Biosciences, St. Louis, MO, USA). Western Blot Analysis The lysate containing an approximate amount

(7) Chloroquine Protects Human Corneal Epithelial Cells from Desiccation Stress Induced Inflammation without Altering the Autophagy FluxBioMed research internationalJanuary 1, 2018Shivakumar S, Panigrahi T, Shetty R, Subramani M, Ghosh A, Jeyabalan N.CQ/CsA using RIPA lysis buffer and nuclear/cytoplasmic extraction kit respectively (Cat no-786-489 and 786-182, G-Biosciences, USA). Protease and phosphatase inhibitors [(phosphostop cat no0490687001, cOmplete EDTA

(8) NSD1 Mitigates Caspase-1 Activation by Listeriolysin O in MacrophagesPLoS ONESeptember 18, 2013Olivia S. Sakhon, Kaitlin A. Victor, Anthony Choy, Tokuji Tsuchiya, Thomas Eulgem, Joao H. F. PedraNuclear protein was extracted using the G-Biosciences: Nuclear and Cytoplasmic Extraction Kit (Catalog# 786–182). The protocol was scaled down appropriately and extraction was done according to the protocol provided.

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Nuclear & Cytoplasmic Extraction Kit from G Biosciences

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Nuclear & Cytoplasmic Extraction Kit from G Biosciences

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