Ribonucleotide Solution Mix from New England Biolabs

Product Specs
ItemRibonucleotide Solution Mix
CompanyNew England Biolabs
Price $317.00 Inquire
Catalog NumberN0466L
Quantity50 µmol
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) Ribonucleotide synthesis by NME6 fuels mitochondrial gene expressionThe EMBO journalSeptember 18, 2023Grotehans N, McGarry L, Nolte H, Xavier V, Kroker M, Narbona-Pérez ÁJ, Deshwal S, Giavalisco P, Langer T, MacVicar T.FBS,Gibco,26400‐044 Galactose,Serva,22020.02 Glucose,Sigma,G7021 Glutamine,Gibco,25030‐081 Sodium Pyruvate,Gibco,11360 rNTPs,New England BioLabs,N0466S dNTPs,New England BioLabs,N0446S EmbryoMax 100× Nucleosides,Millipore,ES‐008‐D Adenosine,Sigma,A4036 Guanosine,Sigma,G6264 Cytidine,Sigma,C4654 Uridine,Sigma,U3003 Lipofectamine

(22) Double-stemmed and split structural variants of fluorescent RNA Mango aptamersRNASeptember 1, 2023Jeremy Herrera-Gutierrez, Steven J. Burden, Sarah E. Kobernat, Nicholas H. Shults, Mark Smith, Daniel Fologea, Eric J. Haydenadded to a transcription reaction containing 1× T7 buffer, 2.5 mM ribonucleotide triphosphate mix (NEB: N0466S), and ∼800 units of T7 RNA polymerase (Thermo Fisher Scientific: EP0113). RNA was purified on a 12%

(23) Single-cell massively-parallel multiplexed microbial sequencing (M3-seq) identifies rare bacterial populations and profiles phage infectionNature MicrobiologyAugust 31, 2023Bruce Wang, Aaron E. Lin, Jiayi Yuan, Katherine E. Novak, Matthias D. Koch, Ned S. Wingreen, Britt Adamson, Zemer Gitailibraries were mixed with 8 μl 5× transcription buffer (Thermo Fisher, EP0112), 6 μl 2.5 mM rNTPs (NEB, N0466L), 1.5 μl of T7 RNA polymerase (Thermo Fisher, EP0112) and 1 μl of RNase-Out. Reactions were incubated

(24) Bacterial drug-resistance and viability phenotyping upon disinfectant exposure revealed by single-nucleotide resolved-allele specific isothermal RNA amplificationJournal of Hazardous MaterialsApril 15, 2023Xinyu Liao, Xuhan Xia, Hao Yang, Yulin Zhu, Ruijie Deng, Tian Dingdeoxynucleotide (dNTP) solution mix (10 mM) (NO. N0447S), and ribonucleotide (rNTPs) solution mix (25 mM) (NO. N0466S) were procured from New England Biolabs (Ipswich, MA, USA). MgCl2 (1 M) (NO. M1028), KCl (1 M) (NO.

(25) Rapid, adaptable and sensitive Cas13-based COVID-19 diagnostics using ADESSONature CommunicationsDecember 1, 2022Beatrice Casati, Joseph Peter Verdi, Alexander Hempelmann, Maximilian Kittel, Andrea Gutierrez Klaebisch, Bianca Meister, Sybille Welker, Sonal Asthana, Salvatore Di Giorgio, Pavle Boskovic, et al.RNase inhibitor (ThermoFisher Scientific, #AM2694), 0.4 μl of rNTP solution mix (25 mM each, NEB, #N0466), 0.3 μl of NxGen T7 RNA Polymerase (Lucigen, #30223-2) and 0.5 μl of MgCl2 (120 mM). 1 μl of the RT-RPA-amplified

(26) Polyvalent guide RNAs for CRISPR antiviralsiScienceNovember 18, 2022Rammyani Bagchi, Rachel Tinker-Kulberg, Mohammad Salehin, Tinku Supakar, Sydney Chamberlain, Ayalew Ligaba-Osena, Eric A. JosephsT7 RNA polymerase,New England Biolabs,Cat#M0251S Ribonucleotide Solution Mix,New England Biolabs,Cat#N0466S SuperScript™ III First-Strand Synthesis System,Invitrogen,Cat#18080051 Leptotrichia wadeii Cas13a

(27) Dual-gene detection in a single-tube system based on CRISPR-Cas12a/Cas13a for severe fever thrombocytopenia syndrome virusFront MicrobiolNovember 3, 2022Yating Zhu, Chen Xing, Li Yang, Qian Li, Xiaofeng Wang, Jing Zhou, Cong Zhang, Cuiping Ren, Fahu Liu, Jun He, et al.μl MgCl2 (1 M; Invitrogen, cat. no. AM9530G), 2 μl Ribonucleotide Solution Mix (25 mM; NEB, cat. no. N0466L), 2 μl LwaCas13a (1 μM; Genscript), 1 μl Cas13a-crRNA (1 μM), 0.1 μl T7 RNA polymerase (50 U/μl, Lucigen,

(28) Highly multiplexed selection of RNA aptamers against a small molecule libraryPLOS ONESeptember 15, 2022Brent Townshend, Matias Kaplan, Christina D. Smolketwo separate 1 ml transcription reactions. Each transcription reaction consisted of 9 mM rNTPs (NEB N0466), 10 mM Dithiothreitol (Invitrogen), and 5 U/μl T7 RNA polymerase (NEB M0251) in 1x RNAPol buffer (NEB).

(29) A transcriptional roadblock protects yeast centromeresNucleic Acids ResearchAugust 12, 2022Sabrine Hedouin, Glennis A Logsdon, Jason G Underwood, Sue Bigginssplit into two tubes and resuspended in 20 μl of Buffer L supplemented with 0.5 mM rNTP mix (NEB, #N0466S), 2 μM spermidine (Sigma, #S2501) and 20 U of RNase inhibitor murine (NEB, #M0314L). One tube had an

(30) A suite of PCR-LwCas13a assays for detection and genotyping of Treponema pallidum in clinical samplesNature CommunicationsAugust 9, 2022Wentao Chen et al.LwCas13a reaction contains 40 mM Tris-HCl (pH 7.5), 9 mM MgCl2, 1 mM rNTPs (New England Biolabs, MA, USA; N0466L), 2000 U/ml Murine RNase inhibitor (New England Biolabs, MA, USA; M0314L), 1500 U/ml T7 RNA Polymerase

(31) CRISPR-Cas13a cascade-based viral RNA assay for detecting SARS-CoV-2 and its mutations in clinical samplesSensors and Actuators B: ChemicalJuly 1, 2022Yuxi Wang et al.(Waltham, USA). ProtoScriptⅡ Reverse Transcriptase (200 U/μL, #M0368S), dNTPs (#N0447S) and rNTPs mixes (#N0466S) were procured from NEB (Ipswich, USA). Hifair® Ⅲ 1st Strand cDNA Synthesis SuperMix for qPCR and 10000

(32) Chemoselective restoration of para-azido-phenylalanine at multiple sites in proteinsCell Chemical BiologyJune 1, 2022Pol Arranz-Gibert, Koen Vanderschuren, Adrian Haimovich, Anushka Halder, Kallol Gupta, Jesse Rinehart, Farren J. IsaacspH8.0, 0.01% Triton X, 30 mM MgCl2, 2 mM Spermidine, 1 mM DTT. For a 100 μL reaction, 4 mM riboNTPs (N0466S, NEB), 100U T7 (M0251S, NEB) and 1-2 μM dsDNA template were added. The reaction was incubated at 37°C

(33) Highly Sensitive Detection Method for HV69-70del in SARS-CoV-2 Alpha and Omicron Variants Based on CRISPR/Cas13aFrontiers in Bioengineering and BiotechnologyApril 12, 2022Mengwei Niu, Yao Han, Xue Dong, Lan Yang, Fan Li, Youcui Zhang, Qiang Hu, Xueshan Xia, Hao Li, Yansong Sunbe preserved by Academy of Military Medical Sciences. Reagents and Instruments NTP mix (art. No. N0466S), and T7 transcription kit (art. No. E2050S) and T7 RNA polymerase (art. No. E2050S), and RNase inhibitors

(34) CryoEM Structures of MDA5-dsRNA Filaments at Different Stages of ATP HydrolysisSSRN Electronic JournalApril 5, 2022Qin Yu, Kun Qu, Yorgo ModisTocris Bioscience 4287 Polyethylenimine Polysciences 24765 Ribonucleotide Solution Mix New England Biolabs N0466 Superdex 200 Increase 10/300 GL column GE Healthcare 28990944 T7 RNA Polymerase New England Biolabs M0251L Critical

(35) Degenerate sequence-based CRISPR diagnostic for Crimean–Congo hemorrhagic fever virusPLOS Neglected Tropical DiseasesMarch 10, 2022Hongzhao Li, Alexander Bello, Greg Smith, Dominic M. S. Kielich, James E. Strong, Bradley S. Pickering(ThermoFisher Scientific, 15630080), MgCl2 (ThermoFisher Scientific, AM9530G), rNTP mix (New England BioLabs, N0466L), NxGen T7 RNA Polymerase (Lucigen, 30223–1) and RNase Alert v2 (ThermoFisher Scientific, 4479768).

(36) Building an RNA-Based Toggle Switch Using Inhibitory RNA AptamersACS Synthetic BiologyFebruary 18, 2022Alicia Climent-Catala, Thomas E. Ouldridge, Guy-Bart V. Stan, Wooli Baeprepared with 200 ng of each DNA template, 1X RNA polymerase Buffer (NEB #B9012S), 8 mM rNTPs (NEB #N0466S), 250 U T7 RNA Polymerase (NEB #M0251L), and/or 100 U SP6 RNA Polymerase (NEB #M0207L), 15 μM of DFHBI-1T

(37) Study on RNAi-based herbicide for Mikania micranthaSynthetic and Systems BiotechnologyDecember 1, 2021Jiantao Mai, Lingling Liao, Rongsong Ling, Xiaolong Guo, Jingying Lin, Beixin Mo, Weizhao Chen, Yu YuChina). The T4 DNA ligase reaction buffer (Code No. B0202S) and ribonucleotide solution mix (Code No. N0466L) were purchased from New England Biolabs (MA, USA). Qubit RNA BR assay kit-100 assays was purchased

(38) Engineering SARS-CoV-2 using a reverse genetic systemNature ProtocolsMarch 1, 2021Xuping Xie, Kumari G. Lokugamage, Xianwen Zhang, Michelle N. Vu, Antonio E. Muruato, Vineet D. Menachery, Pei-Yong ShiR3150S) Restriction enzyme SnaBI (NEB, cat. no. R0130L) Ribonucleotide solution mix (rNTP solution mix; NEB, cat. no. N0466L) SOC outgrowth medium (10 mL; Invitrogen, cat. no. 15544034) Sodium acetate (Sigma-Aldrich, cat. no.

(39) Computationally Reconstructing Cotranscriptional RNA Folding Pathways from Experimental Data Reveals Rearrangement of Non-Native Folding IntermediatesMolecular CellFebruary 1, 2021Angela M Yu, Paul M. Gasper, Luyi Cheng, Lien B. Lai, Simi Kaur, Venkat Gopalan, Alan A. Chen, Julius B. Lucks,New England Biolabs,Cat#M0551S Gln111 (EcoRI E111Q Mutant),Lab preparation,N/A Ribonucleotide Solution Set,New England Biolabs,Cat#N0466 Benzoyl Cyanide,Sigma-Aldrich,Cat#115959 Dimethyl Sulfoxide,Sigma-Aldrich,Cat#276855 TRIzol Reagent,Thermo Fisher

(40) Sequential Activation of Guide RNAs to Enable Successive CRISPR-Cas9 ActivitiesMolecular CellJanuary 1, 2021Ryan Clarke, Alexander R. Terry, Hannah Pennington, Cody Hasty, Matthew S. MacDougall, Maureen Regan, Bradley J. Merrillendonuclease,NEB,ER1011 Calf intestinal phosphatase (CIP),NEB,M0525 Ribonucleotide solution mix,NEB,N0466 T7 RNAP,Dr. Miljan Simonovic,N/A 0.1% Gelatin,Millipore,ES-006-B Knockout DMEM,GIBCO,10829–018 L-Glutamine,GIBCO,25030–081 HEPES,Thermo

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