Ribonucleotide Solution Mix from New England Biolabs

Product Specs
ItemRibonucleotide Solution Mix
CompanyNew England Biolabs
Price $79.00 Inquire
Catalog NumberN0466S
Quantity10 µmol
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Protocol for gene knockdown in Trichoplax adhaerens using a silica nanoparticle-mediated RNA interference techniqueSTAR ProtocApril 2, 2026Qiuyao Guo, Wanqing Li, Weiqiang Zheng, Meng Qiu, Chengtian ZhaoqPCR Master Mix,Vazyme,Cat#Q711 Ribonucleotide Solution Mix,New England Biolabs (https://www.neb.cn/),Cat#N0466S T7 RNA Polymerase,New England Biolabs,Cat#M0251S RNase Inhibitor,New England Biolabs,Cat#M0314S DNase

(2) Transcriptional competence defines the heterochromatin nucleating potential of isolated MSR unitsNat CommunMarch 20, 2026Yi-Hsuan Lo, Nicholas Shukeir, Galina Erikson, Raghu Ram Edupuganti, Reagan Ching, Deepika Puri, Lisa Jerabek, Ramin Shiekhattar, Thomas JenuweinCountess 3 Cell Counter (Thermo Fisher Scientific). 1 × 107 nuclei were incubated with 25 mM rNTPs (NEB, N0466S) and 160 U T7 RNAP (NEB, M0658S) at 37 °C for 1 h. The reaction was stopped and RNA was extracted using

(3) m6A reader Ythdf proteins control retrotransposon B2 repeat expression and safeguard early embryo developmentThe EMBO JournalMarch 11, 2026Ruibao Su, Di Gao, Zongchang Du, Tie-Gang Meng, Chao Li, Lei-Ning Chen, Xiaoting Lin, Changchang Cao, Li-Hua Fan, Yanbin Dong, et al.,Thermo Scientific,AM9624 dNTP Solution Mix,New England BioLabs,N0447 Ribonucleotide Solution Mix,New England BioLabs,N0466 Qubit RNA HS Assay Kit,Thermo Scientific,Q32852 Qubit dsDNA HS Assay Kit,Thermo Scientific,Q32854 Glycogen,Thermo Scientific

(4) Oxidized MIF is an Alzheimer’s disease drug target relaying external risk factors to tau pathologyCell Reports MedicineJanuary 20, 2026Andreas Müller-Schiffmann, Felix Torres, Anatoliy Kitaygorodskyy, Anand Ramani, Argyro Alatza, Sarah K. Tschirner, Julien Orts, Arthur Haltrich, Ingrid Prikulis, Shaofeng Yu, et al.milk,Oxoid,LP0033B ProLong Gold with DAPI,EMS,17984–24 Mowiol,Sigma-Aldrich,81381 Ribonucleotide triphosphate mix,NEB,N0466S Creatine phosphate,Sigma-Aldrich,2380 Creatine kinase,Sigma-Aldrich,C3755 Instant Blue Coomassie

(5) A handheld microfluidic manifold for massively multiplexed CRISPR-based nucleic acid detectionbioRxivAugust 12, 2025Sijbren Kramer, Ryungeun Song, Yujia Huang, Soonwoo Hong, Ibrahim Motlani, Howard A. Stone, Cameron Myhrvoldwith 60mM KCl and 3.5% PEG-8000), 0.3 mM of each ribonucleotide triphosphate (rNTP) (NEB, cat. no. N0466L), 1 U μl–1 murine RNase inhibitor (NEB, cat. no. M0314L), 45 nM Lwa Cas13a purified as described below,

(6) A streamlined CRISPR-based test for tuberculosis detection directly from sputumSci AdvAugust 8, 2025Alexandra G. Bell, Owen R. S. Dunkley, Nisha H. Modi, Yujia Huang, Soleil Tseng, Robert Reiss, Naranjargal Daivaa, J. Lucian Davis, Deninson Alejandro Vargas, Padmapriya Banada, et al.7.5) and 1 mM dithiothreitol], 0.3 mM of each ribonucleotide triphosphate (rNTP) (New England Biolabs, N0466L), T7 RNAP (1 U/μl; Biosearch Technologies Inc. (Lucigen, NC2089983), 62.5 nM FAM 6U quenched reporter

(7) Structural stabilization of the intrinsically disordered SARS-CoV-2 N by binding to RNA sequences engineered from the viral genome fragmentNat CommunJuly 15, 2025Sara Landeras-Bueno, Chitra Hariharan, Ruben Diaz Avalos, Andrew S. Norris, Dalton T. Snyder, Kathryn M. Hastie, Stephanie Harkins, Michelle Zandonatti, Roshan R. Rajamanickam, Eduardo Olmedillas, et al.gene block as a template, T3 polymerase and RNApol reaction buffer (NEB M0378S), 0.5 mM rNTP Mix (NEB #N0466S), 5 mM DTT, and RNAse inhibitors. The sample was incubated for 3 h at 37 °C. The template DNA was digested

(8) A rapid LASV detection method based on CRISPR-Cas13a and recombinase aided amplification with special lateral-flow test stripsSci RepJuly 1, 2025Tong Wei, Yujie Yan, Mengwei Niu, Xue Dong, Hao Li, Yansong Sun, Yunzhi Facorresponding inactivated viruses stored in our laboratory. Main reagents and devices NTP mix (art. No. N0466S), and T7 transcription kit (art. No. E2050S) and T7 RNA polymerase (art. No. E2050S), and RNase inhibitors

(9) Generation, Propagation, and Titering of Dicistrovirus From an Infectious CloneBIO-PROTOCOLFebruary 20, 2025Junzhou Shen, Jibin Sadasivan, Eric Jan(Thermo Fisher Scientific, catalog number: EO0382) 48. Ribonucleotide solution mix (New England Biolabs, catalog number: N0466) 49. RNA Clean-Up kit (New England Biolabs, catalog number: T2040) 50. SafeView DNA stains (Applied

(10) Field-deployable porcine epidemic diarrhea virus diagnostics utilizing CRISPR-Cas13aVirulenceDecember 31, 2024Yuanyuan Wang, Dalin He, Weihua Li, Yaqin Dong, Linlin Fang, Deju Liu, Yi Tang, Shaobo XiaocrRNA, RPA pre-amplification reaction sample, MgCl2, recombinant nucleotide triphosphates (cat. no. N0466L) and T7 polymerase (cat. no. E2050S), RNase inhibitor (New England Biolabs, cat. no. M0314S), buffer

(11) Reconstructing signaling history of single cells with imaging-based molecular recordingbioRxivOctober 15, 2024Kai Hao, Mykel Barrett, Zainalabedin Samadi, Amirhossein Zarezadeh, Yuka McGrath, Amjad Askaryincubated with 50 μl of T3 transcription mix at 37 °C for 3 h, which consisted of 0.5 mM NTP (NEB, N0466S), 0.8 U/μl RNase inhibitor (NEB, M0314S), 5 units/μl T3 RNA polymerase (NEB, M0378S) and 1 × RNAPol reaction buffer

(12) Simultaneous probing of transcription, G-quadruplex, and R-loopMethods in EnzymologyOctober 10, 2024Tapas Paul, Leya Yang, Chun-Ying Lee, Sua Myong(Sigma-Aldrich, P3911) RNase Inhibitor, Murine (NEB, M0314S) Ribonucleotide Solution Mix (rNTP) (NEB, N0466S) RNase H (NEB, M0297S) Sodium bicarbonate (Sigma-Aldrich, #S5761) Sodium chloride (Sigma-Aldrich,

(13) Short-term cold exposure induces persistent epigenomic memory in brown fatCell MetabolismAugust 6, 2024Shin-ichi Inoue, Matthew J. Emmett, Hee-Woong Lim, Mohit Midha, Hannah J. Richter, Isaac J. Celwyn, Rashid Mehmood, Maria Chondronikola, Samuel Klein, Amy K. Hauck, et al.AM2696 Br-UTP,Sigma-Aldrich,Cat# B7166 "Ribonucleotide Solution Set (ATP, GTP, CTP)",New England Biolabs,Cat# N0466 SuperScript III Reverse Transcriptase,Thermo Fisher Scientific,Cat# 18080–051 Deoxynucleotide (dNTP) Solution Mix

(14) Citrate serves as a signal molecule to modulate carbon metabolism and iron homeostasis in Staphylococcus aureusPLOS PathogensJuly 30, 2024Feifei Chen, Qingmin Zhao, Ziqiong Yang, Rongrong Chen, Huiwen Pan, Yanhui Wang, Huan Liu, Qiao Cao, Jianhua Gan, Xia Liu, et al.respectively. Transcription was initiated by the addition of 1 μL nucleotide mixtures (NEB, Catalog no. N0466S), and the reaction mixtures were incubated at 37°C for 15 min. Subsequently, 1U RNase free DNase RQ1

(15) Characterization of the dual regulation by a c-di-GMP riboswitch Bc1 with a long expression platform from Bacillus thuringiensisMicrobiology SpectrumJuly 2, 2024Lu Liu, Dehua Luo, Yongji Zhang, Dingqi Liu, Kang Yin, Qing Tang, Shan-Ho Chou, Jin Heas follows: First, 2 U of E. coli RNAP holoenzyme (M0551S, New England Biolabs), 2 uL of rNTP mix (N0466S, New England Biolabs, 25 mM of each), and 40 U of recombinant RNase inhibitor (2313A, Takara) were mixed

(16) Linearly Amplified Single-Stranded RNA-Derived Transcriptome Sequencing (LAST-seq)BIO-PROTOCOLJune 5, 2024Jun Lyu, Chongyi Chen(New England BioLabs, catalog number: M0212L)Ribonucleotide solution mix (New England BioLabs, catalog number: N0466S)T7 RNA polymerase (New England BioLabs, catalog number: M0658S)DL-dithiothreitol solution (DTT) (MilliporeSigma,

(17) CRISPR/Cas13a-based supersensitive circulating tumor DNA assay for detecting EGFR mutations in plasmaCommunications BiologyMay 28, 2024Li Wang, Xiaosha Wen, Yang Yang, Zheng Hu, Jing Jiang, Lili Duan, Xiaofen Liao, Yan He, Yaru Liu, Jing Wang, et al.included 25 mM Tris HCl, 9 mM MgCl2, 100 ng Cas13a, 20 ng crRNA, 10 mM rNTP (NEB, Shanghai, China, Cat# N0466S), 15 U T7 RNA polymerase (NEB, Cat# M0251S), 8 U RNA inhibitor (NEB, Cat# M0314S), 0.25 μM FAM-labeled

(18) Human PKD1 sequences form R-loop structures in vitroMicroPubl BiolFebruary 2, 2024Agata M Parsons, Kemin Su, Maya Daniels, Gerrit J Bouma, Gregory B Vanden Heuvel, Erik D Larson20-microliter transcription reaction included 400 ng of plasmid, 0.5 mM each rNTP (New England Biolabs, N0466S), 1 X manufacturer buffer, and 100 units of the indicated RNA polymerase, incubated at 37 °C for 1 hour.

(19) A rapid isothermal CRISPR-Cas13a diagnostic test for genital herpes simplex virus infectioniScienceJanuary 19, 2024Xiaona Yin, Hao Luo, Han Zhou, Ziyan Zhang, Yinyuan Lan, Zhanqin Feng, Wentao Chen, Heping Zheng7.5),"Invitrogen, CA, USA",Cat# 15567-027 Ribonucleotide Solution Mix,"New England Biolabs, MA, USA",Cat# N0466L Murine RNase inhibitor,"New England Biolabs, MA, USA",Cat# M0314L T7 RNA Polymerase,"New England Biolabs,

(20) An isothermal CRISPR-based diagnostic assay for Neisseria gonorrhoeae and Chlamydia trachomatis detectionMicrobiology SpectrumDecember 12, 2023Hao Luo, Lihong Zeng, Xiaona Yin, Yuying Pan, Jianjiang Yang, Mingjing Liu, Xiaolin Qin, Zhanqin Feng, Wentao Chen, Heping ZhengCutSmart Buffer (New England Biolabs, MA, USA; B7204), 1 µL of rNTPs Mix (New England Biolabs, MA, USA; N0466 L), 1.25 µL of Murine RNase inhibitor (New England Biolabs, MA, USA; M0314 L), 0.75 µL of T7 RNA Polymerase

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