NotI-HF from New England Biolabs

Product Specs
ItemNotI-HF
CompanyNew England Biolabs
Price $80.00 Inquire
Catalog NumberR3189S
Quantity500 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GC⇓GGCCGC
CGCCGG⇑GC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translationNucleic Acids ResApril 30, 2026Dorthy Fang, John M Babich, Ryan Stanton, Isaac W Vock, Kyrillos Abdallah, Mingyi Zhu, Raj Letchuman, Richard Li, Matthew D Simon, Wendy V Gilbert, et al.with the native T7 promoter mutated to be nonfunctional (TAATACGAgagACTATA) and cut with NotI-HF (NEB, R3189S). Full 5′UTRs were included for 5′UTR m1A modification sites, while CDSs were truncated to reach a similar

(2) Synthetic budding morphogenesis by optogenetic receptor tyrosine kinase signalingbioRxivApril 2, 2026Louis S. Prahl, Ronald Canlla, Aria Zheyuan Huang, Daniel S. Alber, Sandra L. Shefter, Sachin N. Davis, Samuel H. Grindel, Zikang Dennis Huang, Thomas R. Mumford, William Benman, et al.obtained by double restriction digest using MluI-HF® (#R3198S, New England Biolabs) and NotI-HF® (#R3189S, New England Biolabs). CLPIT retroviral vector was obtained by double restriction digest with NotI-HF®

(3) CBP-IDRs regulate acetylation and gene expressionCell RepMarch 24, 2026Katie L. Gelder, Nicola A. Carruthers, Grace Gilbert, Laura J. Harrison, Brychan V. Evans, Thomas I. Evans, Sophie S. Ball, Mark Dunning, Timothy D. Craggs, Alison E. Twelvetrees, et al.GeneAmp dNTP Blend (10mM),ThermoFisher,N8080260 NEBuilder HiFi DNA Assembly reaction,NEB,E2621L NotI-HF,NEB,R3189S SbfI,NEB,R3642 T4 DNA ligase,NEB,M0202S DpnI,NEB,R0176S T4 polynucleotide kinase (PNK),NEB,EK0031 NruI-HF,NEB,R3192S PmlI,NEB,R0532S Quick

(4) Protocol for blastomere injection to generate bilateral hemimosaic Xenopus tadpolesSTAR ProtocolsMarch 20, 2026Vanessa J. Li, Philip M. Kesner, Anne Schohl, David Foubert, Nicholas Benfey, Edward S. RuthazerHOR-272 "0.9% sodium chloride injection USP, 10 mL vial",Pfizer,NDC: 00409-4888-10 NotI,NEB,Cat# R3189S UltraPure Agarose,Life Technologies,Cat# 16500-100 ,, Deposited data,Deposited data,Deposited data ,, Injection

(5) A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based constructMolecular Therapy OncologyMarch 19, 2026Alexandros Kourtesakis, Hiu Nam Hannah Chow, Eileen Bailey, Sandra Horschitz, Ammar Jabali, Rainer Will, Christoph Schifflers, Abigail K. Suwala, Hannah Rohdjess, Melissa Hahn, et al.destination backbones underwent double digestion with MluI (#R3198S, New England Biolabs) and NotI (#R3189S, New England Biolabs). A non-targeting control construct encoding only tdTomato was also generated (referred

(6) Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecovirusesScientific ReportsMarch 18, 2026John A. Frank, Eric Xu Gan, William Brenham Hooper, Isabel Madeleine Ott, Akiko Iwasakiwere synthesized as gene blocks by Twist Bioscience and subcloned into XbaI (NEB R0145S) and NotI (NEB R3189S) double-digested pCAGGS plasmids by Gibson Assembly (NEB HiFi M5520). pTwist-SARS-CoV-2-BA1-S (UFO69279.1),

(7) PFKM governs metabolic shifts throughout skeletal muscle differentiationNature MetabolismFebruary 24, 2026Melissa Campos, Steven T. Nguyen, Xiangduo Kong, Ying Yang, Richard L. Watson, Anastasia Gromova, Catherine R. Livelo, Carolina N. Franco, Julia E. Cabral, Laurence J. Seabrook, et al.(Supplementary Data 1) was generated from a SNAP-GSK3β plasmid30 by using BamHI/NotI restriction enzymes (R3136S/R3189S, New England BioLabs) and the following PFKM primers (Integrated DNA Technologies): forward, 5′-GATCGGATCCACCCATGAAGAGCACCATGCAG-3′;

(8) Mapping the Mutational Landscape for Streptokinase Binding to PlasminogenbioRxivFebruary 4, 2026Srishti Baid, Matthew L Holding, Taylor Laurin, David Seimeiniak, David Ginsburg, Laura M HaynesGln), between the AscI (Product# R0558S, New England Biolabs, Ipswich, MA,USA) and NotI (Product # R3189S, New England Biolabs) restriction sites (Supplementary table 1) using T4 ligase (Product #M1794, Promega,

(9) Notch Signalling Plays a Role in Patterning the Ventral Mesoderm During Early Embryogenesis in Drosophila melanogasterInternational Journal of Molecular SciencesJanuary 27, 2026Marvel Megaly, Gregory Foran, Arsala Ali, Anel Turgambayeva, Tai Sengsouriya, Samantha Berube, Ryan Douglas Hallam, Ping Liang, Aleksandar NecakovCarlsbad, CA, USA) by cutting the cassette out of pUASP-attb-Cry2-CTEV-mCherry using NotI and XbaI (NEB #R3189S and #R0145S, Whitby, ON, Canada) and ligating it to the linearized pAc5.1/V5-His A. The resultant plasmid

(10) Integrative transcriptome-wide association analyses reveal PRKCG-linked GABAergic dysfunction in Fragile X-associated tremor/ataxia syndromeNature CommunicationsJanuary 8, 2026Yulin Jin, Yiqu Cao, Wenjing Ma, Ronghua Li, Yujing Li, Yunhee Kang, Jing Huang, Michael P. Epstein, Xiangxue Guo, Junghwa Lim, et al.Both the PRKCG insert and the pUAST backbone were digested with Not1 and Kpn1 (New England Biolabs, R3189S and R3142S) at 37 °C for 30 min and ligated using T4 DNA ligase (New England Biolabs, M0202) at 16 °C

(11) Sequence-dependent co-condensation of Lsr2 with DNA elucidates the mechanism of genome compaction in Mycobacterium tuberculosisNucleic Acids ResearchJanuary 8, 2026Prakshi Gaur, Thejas Satheesh, Rohit Kumar Singh, Hussain Beig, Sneha Shahu, Saminathan Ramakrishnan, Mansi Srivastava, Shreyasi Neogi, Ayesha Dash, Amit Singh, et al.biotinylated DNA molecules, the 21-kb plasmid with 3-kb AT-rich in the center was digested with NotI (NEB R3189S) and XhoI (NEB R3189S) restriction enzymes, and clean-up was performed to remove non-specific small

(12) Low-order assemblies drive oncogenic RTK fusion signaling without condensationbioRxivDecember 13, 2025David Gonzalez-Martinez, Thomas R. Mumford, Delaney Wilde, Sofia Wissert, Yuzhi (Carol) Gao, Emily Brackhahn, Richard Kriwacki, Elizabeth Rhoades, Lukasz J. BugajDonor DNA was prepared for transfection into HeLa cells by digesting the plasmid with NotI-HF (NEB, #R3189S) and EcoRI-HF (NEB,#R3101S) which were encoded in the 5’ and 3’ of the donor DNA sequence. Following

(13) The N-terminal region of malaria vaccine candidate Plasmodium falciparum asparagine-rich merozoite antigen is immunodominant and targeted by polyreactive antibodiesbioRxivDecember 13, 2025Rolando Garza, Jeffrey M. Marchioni, Jared D. Honeycutt, Nicholas K. Hurlburt, Caroline Torres, Anakaren Garcia, Eva Loranc, Emily Yemington, Dalton Towers, Isaac Ssewanyana, et al.end, respectively. PCR amplified fragments and the parent plasmid were digested using NotI-HIFI (NEB #R3189S) and AscI (NEB #R0558S) according to manufacturer specifications. The digested plasmid was run on a

(14) A commensal bacterium secretes effector proteins to establish population heterogeneity in the gutCell Host & MicrobeDecember 10, 2025Anna P. Zagieboylo, Ran Mo, Bentley Lim, Andrew L. Goodman,New England Biolabs,Cat#M0491L OneTaq DNA Polymerase,New England Biolabs,Cat#M0480L NotI-HF,New England Biolabs,Cat#R3189S PstI-HF,New England Biolabs,Cat#R3140S NcoI-HF,New England Biolabs,Cat#R3193S SalI-HF,New England Biolabs

(15) Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translationbioRxivDecember 8, 2025Dorthy Fang, John M. Babich, Ryan Stanton, Isaac W. Vock, Kyrillos Abdallah, Mingyi Zhu, Richard Li, Matthew D. Simon, Wendy V. Gilbert, Sigrid Nachtergaelewith the native T7 promoter mutated to be nonfunctional (TAATACGAgagACTATA) and cut with NotI-HF (NEB, R3189S). Full 5'UTRs were included for 5'UTR m1A modification sites, while coding sequences were truncated

(16) Depletion of tryptophanyl-tRNA synthetase and tryptophan accumulation triggers p53-dependent apoptosisCell Death DiscoveryDecember 5, 2025Tayyiba Akbar Ali, Mahmoud Izadi, Raheleh Vazehan, Maryam Al-Mansoob, Ehsan Pourkarimicloned to the L4440 RNAi plasmid using molecular cloning and utilizing ApaI (R0114S, NEB) and NotI HF (R3189S, NEB) restriction enzyme [26]. The coding sequence of tph-1 and afmd-2 were amplified using C. elegans

(17) Discovery of an allosteric binding site for anthraquinones at the human P2X4 receptorNat CommunDecember 4, 2025Jessica Nagel, Vigneshwaran Namasivayam, Stephanie Weinhausen, Juan Sierra-Marquez, Younis Baqi, Hashem Ali M. Al Musawi, Aliaa Abdelrahman, Victoria J. Vaaßen, Jonathan G. Schlegel, Lisa Taplick, et al.by full-length cDNA sequencing (Eurofins Genomics, Germany). Plasmids were linearized with NotI-HF (R3189S, New England Biolabs, USA) and purified using the MinElute Reaction Cleanup Kit (Qiagen, Hilden, Germany).

(18) Strategies to Screen and Evaluate Brain Targeting Antibodies Using an iPSC-Derived Blood–Brain Barrier ModelAntibodies (Basel)November 26, 2025Eun Seo Choi, Sophia Sahota, Emily Burnham, Yunfeng Ding, Eric V. Shustatetracycline resistance, using the restriction sites PstI (NEB R3140S, Ipswich, MA, USA) and NotI (NEB R3189S). QuickCIP (NEB M0525S) was used to dephosphorylate the plasmid backbone during restriction digestion,

(19) Sox8 is essential for vertebrate gastrulationEMBO ReportsNovember 10, 2025Sofia Moreira, Artemis G Korovesi, Elias H Barriga(Appendix Fig. S7 and see Methods for further details) to digest the mixture for 4 h with NotI-HF (NEB, # R3189S). Note 14: We usually use a 100 μl digestion volume to increase the chance of obtaining good HuDisCas7-11-msfGFP

(20) A high-throughput screening approach to discover potential colorectal cancer chemotherapeutics: repurposing drugs to identify novel disruptors of 14-3-3 proteinsCell Death & DiseaseNovember 10, 2025Siyi He, Daniel Meister, Samra Khan, Azam Mohammadzadeh, Luis Delgadillo Silva, Guy A. Rutter, John F. Trant, Gareth E. Limand used to replace the mTurquoise of constructed 14-3-3ζ-mTurquoise using AgeI and NotI-HF (NEB; #R3189S). To conjugate Rluc8 to the N-termini of 14-3-3ζ, Rluc8 was subcloned to the original 14-3-3ζ-containing

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(1)

5/1/2018Diagnostic Restriction Mapping Cloned Plasmid Constructs From E.Coli Cells Using NotI-HF Triveni MahendrappaI have constructed a plasmid called pET-evgS of size 7.12 kb. This plasmid was transformed into E. coli DH5 alpha cells and selected on agar plates with selective antibiotic marker. Selected clones ... Read Review

Paired- End diTagging for Transcriptome and Genome Analysis
July 01, 2007

NotI-HF

NotI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (190)

Reviews

Protocols

Paired- End diTagging for Transcriptome and Genome Analysis

NotI-HF from New England Biolabs

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