NotI from New England Biolabs

Product Specs
ItemNotI
CompanyNew England Biolabs
Price $329.00 Inquire
Catalog NumberR0189L
Quantity2500 units
Concentration10-50 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GC/GGCCGC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genomeNucleic Acids ResApril 28, 2026Amruta R Jadhav, Sushmita Sahoo, Yash L Kuber, Krutika Hatwar, Avinash Varanwal, Khushi Tanwar, Shubhankar Palwankar, Gauri Chatrasal, Avinash M Mali, Sharmila A Bapatstrategy. For the full-length and Δ constructs (Δ1 and Δ2) in pUAST, KpnI-HF (NEB, #R3142S) and NotI (NEB, #R0189S) were employed; Δ UP (upstream region) was achieved using NdeI (NEB, #R0111S) and BglII (NEB, #R0144S);

(2) STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondriaThe EMBO JournalFebruary 11, 2026Yolanda Orantos-Aguilera, Irene Sanchez-Lopez, Carlos Pascual-Caro, Patricia Gómez-Suaga, Estela Area-Gomez, Eulalia Pozo-Guisado, Jorge Montesinos, Francisco Javier Martin-Romerokit,BioTools,4542 Nitrocellulose Blotting Membrane,AmershamTM ProtranTM,10600001 NotI,New England Biolabs,R0189 NuPAGE-LDS sample buffer,Invitrogen,NP0007 PBS,Fisher BioReagents,BP3994 Percoll®,GE Healthcare,17-0891-02 Phenylmethylsulphonyl

(3) A genome-scale single-cell CRISPRi map of trans gene regulation across human pluripotent stem cell linesCell GenomicsFebruary 11, 2026Claudia Feng, Elin Madli Peets, Yan Zhou, Luca Crepaldi, Sunay Usluer, Alistair Dunham, Jana M. Braunger, Jing Su, Magdalena E. Strauss, Daniele Muraro, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, BbsI,NEB,R0539L NotI,NEB,R0189S EcoRV-HF,NEB,R3195S ,, Critical commercial assays,Critical commercial assays,Critical commercial

(4) Modular gene tagging in C. elegansResearch SquareFebruary 10, 2026Erik Jorgensen, Kevin Hefel, Kevin Kruse, Soren B. Jorgensen, Kam Hoe Jorgensen, Ryan Stolley, Matthew S. Stolley, Matthew Jorgensento generate pAJH103, after which we cut and added SapI sites by restriction digest with NotI (NEB® R0189) and annealed overhanging oligos (pAJH110). Finally Golden Gate assembly was used to assemble Pmex-5::B3::let-858

(5) Long-read sequencing reveals increased isoform diversity in key transcription factor effectors of intercellular signalling at the invertebrate-vertebrate transitionBMC BiologyJanuary 24, 2026Nuria P. Torres-Aguila, Marika Salonna, Sebastian M. Shimeld, Stefan Hoppler, David E. K. Ferrierresistance and their composition was confirmed by enzyme digestion with NotI (New England Biolabs, R0189) and Sanger sequencing (Oxford Zoology service, Eurofins service). The sequences were compared against

(6) Modular gene tagging in C. elegansbioRxivJanuary 18, 2026Adam Hefel, Kevin Kruse, Kaden Wall, Soren B. Jorgensen, Kam Hoe Ng, Ryan Stolley, Matthew S. Rich, Erik M. Jorgensento generate pAJH103, after which we cut and added SapI sites by restriction digest with NotI (NEB® R0189) and annealed overhanging oligos (pAJH110). Finally Golden Gate assembly was used to assemble Pmex-5::B3::let-858

(7) Complexoform-restricted covalent TRMT112 ligands that allosterically agonize METTL5Nature Chemical BiologyJanuary 8, 2026F. Wieland Goetzke, Steffen M. Bernard, Cheng-Wei Ju, Jonathan Pollock, Kristen E. DeMeester, Jacob Gross, Gabriel M. Simon, Chuan He, Bruno Melillo, Benjamin F. CravattSupplementary Table 3). The PCR product was cloned into a pET21a vector using NdeI (NEB, R0111S) and NotI (NEB, R0189S) restriction and Gibson assembly (NEB, E2621S). His–METTL5_Gibson_FWD: taactttaagaaggagatatacATATGCACCACCACCACCACCACGAAAATTTGTATTTCCAGTCAATGAAGAAGGTACGTCTG His–METTL5_Gibson_REV:

(8) Efficient Fluorescent Labeling of Human Trophoblast Stem Cells via a CRISPR/Cas9-Mediated Knock-In Approach in a Safe Harbor LocusBIO-PROTOCOLJanuary 5, 2026Hengshan Zhang, Jie Zhou, Morgan Orsolini, Anthony Zhao, Askar Takhirov, Danny J. SchustXho I (New England Biolabs, catalog number: R0146S) or Not I (New England Biolabs, catalog number: R0189S) restriction enzymes, which have unique cutting sites for the target plasmid and donor plasmid, respectively. b.

(9) Lymphodepleting chemotherapy potentiates neoantigen-directed T cell therapy by enhancing antigen presentationCell Rep MedDecember 16, 2025Shira Sagie, Tomer Babu, Chen Weller, Claire Tabachnik, Ido Livneh, Nele P. Quast, Nofar Gumpert, Alina Shomuradova, Matthew I. Raybould, Ronen Levy, et al.Aldrich,Cat#78830 octyl-b-D glucopyranoside,Sigma Aldrich,Cat#O8001 KAPA HiFi HotStart ReadyMix,Roche,Cat# KK2602 Not1,NEB,R0189S XBA1,NEB,R0145T CutSmart,NEB,#B6004 TurboFect,Thermo Fisher Scientific,Cat#R0531 DSP,Thermo Fisher Scientific

(10) Molecular interactions of Chd8 in mouse brain highlights a role in chromatin-associated RNA processingbioRxivDecember 12, 2025Emily Smith, Cesar P. Canales, Mathew W. Kenaston, Nicolas Seban, Ayanna A. Wade, Ethan Fenton, Cory Ardekani, Stephanie Lozano, Nickolas C. Chu, Daniela Perla, et al.verified, the GFP sequence was removed using a double restriction digest with enzymes NotI and PmeI (NEB, R0189S and R0560S). The plasmid was reassembled using In-Fusion, introduced into bacteria, and sequence verified

(11) Generation of a scFv Derived from an IgM-Producing Hybridoma for the Detection of REST Expression in Premalignant Lesions and Invasive Squamous Cell CarcinomaInt J Mol SciDecember 11, 2025Cynthia Rodríguez-Nava, Karen Cortés-Sarabia, Lidia Riaño-Umbarila, Baltazar Becerril-Luján, Yolanda Medina-Flores, Olga Mata-Ruíz, Lourdes Lloret-Sánchez, Berenice Illades-Aguiar, Luz Del Carmen Alarcón-Romero, Carlos Ortuño-Pinedawith the enzymes SfiI and NotI (both from New England Biolabs, Ipswich, MA, USA, Cat# R0123S and Cat# R0189S), and ligated to the plasmid pSyn1 previously cut with the same restriction enzymes using T4 ligase. 4.4.

(12) Evolution of the HIV-1 integration site landscape and inducible reservoir in early-treated peoplePLOS PathogensNovember 25, 2025Tine Struyve, Marion Pardons, Jozefien De Clercq, Liesbet Termote, Laurens Lambrechts, Ytse Noppe, Mathias Lichterfeld, Sofie Rutsaert, Linos Vandekerckhoveextracted genomic DNA from CD4 T cells, 2 uL Advantage2 10x buffer (Takara Bio, #639202), 1 uL NotI (NEB, #R0189L) and nuclease-free water to a final volume of 20 uL (30 min at 37°C). Next, linear extension was performed

(13) Effects of SADS‐CoV accessory proteins NS3a, NS7a, and NS7b on viral pathogenicity: A multi‐omics investigationiMetaOmicsSeptember 1, 2025Xiaoling Yan, Xiaoli Zhang, Ling Zhou, Xiaoya Zhao, Qianniu Li, Tian Lan, Jun Fu, Jingyun MaS1). The p15A‐cm‐CAGGS‐HA‐BGH‐polyA plasmid was linearized using the restriction enzyme NotI (NEB, R0189L). PCR amplification and enzyme digestion products were electrophoresed on a 1% agarose gel, and the

(14) Genetic and behavioral analyses suggest that larval and adult stages of Lucilia cuprina employ different sensory systems to detect rotten beefParasit VectorsJuly 9, 2025Juan P. Wulff, Rachel K. Laminack, Maxwell J. Scottplus restriction cutting sites for the enzymes XhoI (NEB, cat. no. R0146S) and NotI (NEB, cat. no. R0189S), was acquired, cloned within the plasmid pUCIDT-AMP GoldenGate (IDT). The ZsGreen fluorescent marker

(15) Tailoring a novel colorectal cancer stem cell-targeted therapy by inhibiting the SMYD3/c-MYC axisSignal transduction and targeted therapyJune 30, 2025Lepore Signorile M, Di Nicola E, Forte G, Sanese P, Fasano C, Disciglio V, De Marco K, Latrofa M, De Cecco L, Ficorilli M, et al.cloned into the p3xFLAG-CMV14 empty plasmid (E7908, Sigma-Aldrich) linearized with NotI and BamHI (R0189, and R0136, New England Biolabs, respectively). The p3xFLAG CMV14 c-MYC-K158A and p3xFLAG CMV14 c-MYC-K163A

(16) The lincRNA Pantr1 is a FOXG1 target gene conferring site-specific chromatin binding of FOXG1Nucleic Acids ResJune 23, 2025Fabian Gather, Tudor Rauleac, Ipek Akol, Ganeshkumar Arumugam, Camila L Fullio, Teresa Müller, Dimitrios Kleidonas, Ruth Geiss-Friedlander, Andre Fischer, Andreas Vlachos, et al.Normal donkey serum,017–000-121,Jackson Immuno Research,United Kingdom Normal goat serum,G6767,Sigma-Aldrich/Merck,Germany Not1,R0189S,New England Biolabs,USA OPTIMEM,31985–062,"Gibco, Thermo Scientific",USA "PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

(17) GPI transamidase complex is required for primordial germ cell migration and development in zebrafishJournal of Molecular Cell BiologyJune 12, 2025Weiying Zhang, Yaqi Li, Jing Chen, Likun Yao, Bingjie Zhang, Lin Zhang, Boqi Liu, Weimin Shen, Anming Meng, Xiaotong Wusynthesis For making gfp-nos3-3′UTR mRNA, the construct pCS2-gfp-nos3-3′UTR was linearized by NotI (NEB, R0189L) digestion. Capped mRNAs were synthesized by using T7/Sp6 mMessage mMachine Kit (Ambion, AM1344 and

(18) Orthogonal replication with optogenetic selection evolves yeast JEN1 into a mevalonate transporterMolecular Systems BiologyJune 11, 2025Scott A Wegner, Virginia Jiang, Jeremy D Cortez, José L Avalosmix,Thermo,F160S T4 Ligase,NEB,M0202S XmaI,NEB,R0180S AscI,NEB,R0558S MreI,Thermo,ER2021 NotI,NEB,R0189S XhoI,NEB,R0146S DpnI,NEB,R0176S Miniprep kit,Epoch Life Science,2160-050 E.N.Z.A. gel extraction

(19) Papillomavirus-like particles as vectors for ex vivo gene therapy of the skinMolecular Therapy Nucleic AcidsJune 10, 2025Francesco Diversi, Juliette Dabin, Elisa Mazza, Mirko Rinaldin, Fernanda de Castro Reis, Jamie A. Hackett, Paul A. Heppenstalldirectly used for producing PVLPs. For this reason, it was digested with XhoI (R0146, NEB) and NotI (R0189, NEB) restriction enzymes for 16 h in NEBuffer r3.1. The digestion mix was separated by electrophoretic

(20) Synergistic optimization enhancing the precision and efficiency of cytosine base editors in poplarCommun BiolJune 10, 2025Han Liu, Mengyu Zhang, Leiqian Sun, Yu Peng, Yu Sun, Yawei Fan, Hui Li, Di Liu, Hai Luas follows: The PYPQ265 vector was digested with XmaI (NEB, catalog #R0180S) and NotI (NEB, catalog #R0189S) enzymes. A gene encoding UGI-T2A-MS2-UGI was synthesized by Tsingke and was codon-optimized for poplar.

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Paired- End diTagging for Transcriptome and Genome Analysis
July 01, 2007

NotI

NotI from New England Biolabs

Sequence

New England Biolabs

Citations (190)

Reviews

Protocols

Paired- End diTagging for Transcriptome and Genome Analysis

NotI from New England Biolabs

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