PNGase F (Glycerol-free) from New England Biolabs

Product Specs
ItemPNGase F (Glycerol-free)
CompanyNew England Biolabs
Price $214.00 Inquire
Catalog NumberP0705S
Quantity15000 units
ApplicationsProteomics & Glycomics
Temperature4 C
TypeGlycosidases

Specifications/Features

Storage:
Storage Conditions:
20 mM Tris-HCl
50 mM NaCl
5 mM Na2EDTA
pH 7.5 @ 25°C

Storage Temperature: 4°C
Store at 4°C, do not freeze.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) α-Halothioamide warheads with enhanced cysteine reactivity and specificity for covalent protein labellingNature CommunicationsMay 14, 2026László Petri, Ronen Gabizon, Nikolett Péczka, Péter Ábrányi-Balogh, József Simon, Tímea Imre, György G. Ferenczy, Nir London, György M. KeserűTrastuzumab samples (n = 1) were deglycosylated with 1 μL PNGase F (glycerol free; New England Biolabs GmbH, P0705) at 37 °C overnight. Chromatographic separation was carried out on a PLRP-S, 1000 Å, 8 µM, 150 mM ×

(2) A posttranslational proteomic survey of a single anatomically preserved human 20‐week postconception brainJournal of AnatomyMay 3, 2026S. Bandiera, H. Bogetofte, P. Jensen, R. Hussain, S. A. Nischal, L. Rihova, G. J. Clowry, M. R. Larsen, Z. Molnár, B. C. Carlyleresolubilized in 150 μL 100 mM HEPES (pH 8.5). The sample was incubated with PNGaseF (New England Biolabs #P0705L) and Sialidase A (Agilent Technologies #GK80040) at 37°C ON. The next day, the TiO2‐enriched peptides

(3) Structurally exclusive Teneurin complexes orchestrate divergent programs in early cortical developmentNature CommunicationsApril 16, 2026Miguel Berbeira-Santana, Claudia Peregrina, Kosuke Okuda, Jin Chuan Zhou, Maria Carrasquero-Ordaz, Amy V. Roberts, Anne E. Thomas, Evert Haanappel, Matthieu Chavent, Kamel el Omari, et al.17.5 µg protein present in 20 mM Tris, 300 mM NaCl buffer, were treated with 500 U of PNGaseF (NEB P0705S) for 1 h at 37 °C. Subsequently, the samples were denatured in 4 M Urea/0.1 M ammonium bicarbonate buffer

(4) Impact of afucosylation strategy on antibody function: a comparative study of glycoengineered anti-CD20 antibodies Obinutuzumab and Obinutuzumab betaMAbsApril 13, 2026Qing Shuang, Mingze Sun, Guangzhong Lin, Lunfeng Zhang, Shuo Huang, Qunfang Shen, Lingling Jiang, Lijing Yang, Jicui Sun, Hong Chen, et al.using a 10 kDa or 30 kDa ultrafiltration tube. Following enzymatic release with PNGase F (NEB, Cat. No. P0705L) overnight at 37°C, the N-glycans were ethanol-precipitated. The purified glycans were then fluorescently

(5) Deletion of SPI1 in microglia exacerbates amyloid pathology by impairing microglial response in Alzheimer's disease modelsNeuronApril 1, 2026Byungwook Kim, Mason Douglas Tate, Hande Karahan, H.R. Sagara Wijeratne, Ahmad Daniel Sharify, Selena S. Wang, Justin R. Kim, Md Mamun Al-Amin, Kelly Hartigan, Sunwoo Chung, et al.chloroacetamide. The samples were digested with Lys-C (Promega), then PNGase F (New England BioLabs, cat # P0705L), then Trypsin Platinum (Promega), and quenched with trifluoroacetic acid68,69. The peptides were desalted,

(6) Effects of feeding strategies on culture performance and product quality in NISTCHOnpj Systems Biology and ApplicationsMarch 19, 2026Veronika Schäpertöns, Larissa Hofer, Thomas Berger, Jerneja Štor, Nicolas Marx, Wolfgang Esser-Skala, Thomas Rauter, Dominik Hofreither, Laura Liesinger, Peter Filzmoser, et al.placed in the HPLC auto-sampler for analysis. For the PNGase F digests, 1 μL of PNGase F (500 units, NEB P0705S, PNGase F Glycerol-free, New England Laboratories, Frankfurt am Main, Germany) was added to the remaining

(7) Profiling the pancreatic cancer secretome with metabolic glycoengineeringJournal of Biological ChemistryMarch 1, 2026Kris Dammen-Brower, Stanley Zhu, Christian Agatemor, Safiya Aafreen, Vrinda Dharharma, Christopher T. Saeui, Hui Li, Jian Song, Matthew J. Buettner, Keith R. Kwagala, et al.into three groups: a control group with no glycosidase treatment, PNGase F (New England Biolabs [NEB], P0705S) treated, and Protein Deglycosylation Mixture II (NEB, P6044S)–treated samples following the manufacturer’s

(8) Multi-omic analysis of guided and unguided forebrain organoids reveals differences in cellular composition and metabolic profilesCell Reports MethodsFebruary 23, 2026Marie Sejberg Øhlenschlæger, Pia Jensen, Jesper Foged Havelund, Sissel Ida Schmidt, Fadumo Abdullahi Mohamed, Magdalena Sutcliffe, Sofie Blomberg Elmkvist, Lucrezia Criscuolo, Steven W. Wingett, Ilaria Chiaradia, et al.,Thermo Fisher Scientific,A52046 "Titanium dioxide beads, 5 μm",GL Science,5020–75010 PNGaseF,New England BioLabs,P0705L Sialidase A,Prozyme,GK80040 SPLASH LIPIDOMIX MS standard internal standard mix,Merck,330707-1EA NuPage

(9) Identification of a novel apoB variant in a family exhibiting hypocholesterolemia: Mechanistic insightsJournal of Clinical LipidologyFebruary 20, 2026Rachid Essalamani, Alexandra Evagelidis, Anna Roubtsova, Michael Chong, Nazia Pathan, Guillaume Pare, Evgeniy V. Petrochenko, Mart Reimund, Alan T. Remaley, Lorraine Chalifour, et al.were digested for 90 min at 37°C with endoglycosidase H (endo H; P0702L) or endoglycosidase F (endo F; P0705S) as recommended by the manufacturer (New England BioLabs, ON, Canada). Plasma collection and circulating

(10) Protocol for simultaneous profiling of N- and O-glycans on glycoproteins using a one-pot formatSTAR ProtocFebruary 5, 2026Kevan T. Knizner, Darren T. Gass, Yasuyuki Matsumoto, Uriel Ortega-Rodriguez, John Q. Bettinger, Tongzhong JuA from fetal bovine seruma,New England Biolabs,P6042S Glycerol-Free PNGase Fa,New England Biolabs,P0705L Proteinase Ka,New England Biolabs,P8107S Iodomethanea,Millipore Sigma,289566 LCMS-Grade Watera,Fisher

(11) Antibody-induced internalization and degradation of PLA2R amplifies CD4+ T cell activationTheranosticsJanuary 1, 2026Yuning Liu, Yinyin Li, Hui Zhou, Jieli Yu, Pei Deng, Weiwei Xu, Bingqian Pan, Lei Zhang, Hong Zhou, Jing Zhang, et al.purchased from Sigma-Aldrich. MG-132(2194S, 10 μM) were purchased from Cell Signaling Technology. PNGase-F (P0705S), XbaI (R0145S) and EcoRI (R3101S) were purchased from New England Biolabs; Bafilomycin A1 (Baf-A1,

(12) IgG binding characteristics of ferret Fcγ receptorsmAbsDecember 31, 2025Xiaoxuan Ge, Matthew Stoner, Joshua A. Weiner, Melanee E. Balderas Hernández, Noor Taher, Urjeet S. Khanwalkar, Margaret C. Carpenter, Jiwon Lee, Margaret E. Ackerman10 μL of 100 mM ammonium bicarbonate with heavy water (O18). Glycerol free PNGase (2,500 units, NEB, P0705) resuspended in heavy water (O18) was then added to the samples and incubated at 37°C for 1 hour. The

(13) Development of predictable and high-yield CHO cell line by site-specific integrationJournal of Biological EngineeringDecember 30, 2025Wanjun Lan, Xiaoli Yang, Juanjuan Yao, Lung-JR Lin, Wenzheng Li, Weihai Chen, Jiangping Li, Qi Shen, Kang Li, Huiling Li, et al.ultrafiltration centrifugal filters. N-glycan in glycoprotein was hydrolyzed by N-glycosidase F (PNGase F) (NEB, P0705S) at 37℃ for 2 h. The hydrolyzed N-glycan was recovered through PVDF microplate (Agilent, 200945-100)

(14) Deletion of neuronal Idol ameliorates Alzheimer's disease-related pathologies via APOE receptorsAlzheimers DementDecember 12, 2025Hande Karahan, Kelly Hartigan, Md Mamun Al-Amin, Sutha K. John, Brianne McCord, H. R. Sagara Wijeratne, Dominic J. Acri, Daniel C. Smith, Luke C. Dabin, Hannah M. Rondon Cordero, et al.chloroacetamide. Samples were digested with Lys‐C for 12 h at 37°C, treated with PNGase F (New England BioLabs, P0705L), and digested with Trypsin Platinum (Promega, VA9000) and quenched with trifluoroacetic acid. 29 ,

(15) Multi-omic profiling of intraductal papillary neoplasms of the pancreas reveals distinct expression patterns and potential markers of progressionCancer CellNovember 10, 2025Yuefan Wang, Tung-Shing M. Lih, Jae W. Lee, Takao Ohtsuka, Yuto Hozaka, Mari Mino-Kenudson, Nazmi Volkan Adsay, Claudio Luchini, Aldo Scarpa, Ajay V. Maker, et al.(TFA),Sigma,Catalog: 302031 Triethylammonium acetate buffer,Sigma,Catalog: 90358 PNGaseF,New England Biolabs,Catalog:P0705L Triethylammonium bicarbonate (TEAB),Fisher Scientific,Catalog: 90114 Methanol,Fisher Scientific,Catalog:

(16) Altered Cell Surface N -Glycosylation Implicates Hypersialylation in Breast Cancer Brain MetastasisJournal of Proteome ResearchNovember 7, 2025Judith Nwaiwu, Wenjing Peng, Akhila Reddy, Xue Dong, Parisa Ahmadi, Jingfu Zhao, Yifan Huang, Waziha Purba, Peilin Jiang, Oluwatosin Daramola, et al.spin columns were purchased from Harvard Apparatus (Cambridge, MA). PNGase F (glycerol-free) cat. No. P0705L was purchased from New England Biolabs. Paraformaldehyde was purchased from Fisher Scientific (Fair

(17) Sequencing of Polyclonal Antibodies by Integrating Intact Mass, Middle–Down, and De Novo Bottom–Up Mass SpectrometryMolecular & Cellular ProteomicsNovember 1, 2025Lei Xin, Wenting Li, Shuyang Zhang, Ngoc Hieu Tran, Zheng Chen, Jun Ma, Chao Peng, Ailee Aihemaiti, Kyle Hoffman, Xiyue Zhang, et al.N-linked glycans on the antibody constant region were removed by adding 0.2 μl PNGase F (NEB; 15000U, P0705S) at 37 °C, incubated overnight, dried down, and then resuspended in 0.1% formic acid prior to LC–MS analysis

(18) Spatial N-glycan rearrangement on α5β1 integrin nucleates galectin-3 oligomers to determine endocytic fateNat CommunOctober 27, 2025Massiullah Shafaq-Zadah, Estelle Dransart, Ilyes Hamitouche, Christian Wunder, Valérie Chambon, Cesar A. Valades-Cruz, Ludovic Leconte, Nirod Kumar Sarangi, Jack Robinson, Siau-Kun Bai, et al.Arthrobacter ureafaciens, Roche Ref. 10269611001), glycerol-free PNGase F (New England Biolabs, Ref. P0705L), Alexa488-conjugated PHA-L lectin (Thermofisher, ref. L11270), Alexa488-conjugated SiaFind Pan-specific

(19) Abnormal O-glycan sialylation in the mPFC contributes to depressive-like behaviors in male miceSci AdvOctober 3, 2025Youngsuk Seo, Inwoong Song, Ki Jung Kim, Bomi Chang, Prajitha Pradeep, Woo Suk Roh, Woojin Won, Jinhyeong Joo, Myeongju Kim, Jae Cheol Jeong, et al.by heating at 95°C for 5 min. N-glycans were removed using peptide N-glycosidase F (PNGase F) (NEB, P0705L), and O-glycopeptides were enriched using C18-based solid phase extraction (SPE) (Waters, 186000308),

(20) GlycoRNA-L and glycoRNA-S mediate human monocyte adhesion via binding to Siglec-5Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchOctober 1, 2025Yong Li, Yisong Qian, Evan Huang, Zain Schwarz, Hannah Tai, Katherine Tillock, Tianhua Lei, Xiaofeng Yang, Mingui Fu(Zymo Research, Cat#:E1009), Proteinase K (ThermoFisher Scientific, Cat#:100005393) or PNGase F (NEB, Cat#:P0705) was added into 20 μl of total RNA at 37 °C for 10 mins. The reaction mixture was purified by Zymo column.

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