PNGase F from New England Biolabs

Product Specs
ItemPNGase F
CompanyNew England Biolabs
Price $853.00 Inquire
Catalog NumberP0704L
Quantity75000 units
ApplicationsProteomics & Glycomics
Temperature-20 C
TypeGlycosidases

Specifications/Features

Storage:
Storage Conditions:
20 mM Tris-HCl
50 mM NaCl
5 mM EDTA
50% Glycerol
pH 7.5 @ 25°C

Storage Temperature: -20°C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Identification and Functional Characterization of Novel and Recurrent NTRK1 Variants in Chinese Families With Congenital Insensitivity to Pain With Anhidrosis: A Combined Clinical, Genetic, and Functional StudyEur J NeurolApril 25, 2026Yaqiong Ren, Yue Cao, Fangfang Cheng, Jin Dai, Yuan Zhang, Xinxin Wang, Jiali Chen, Lijun Zhou, Xiaoxiang Song, Hongying WangAssessment To evaluate alterations in protein glycosylation, we employed PNGase F (New England Biolabs, P0704) to treat protein extracts, following the manufacturer's protocol. The deglycosylated and non‐deglycosylated

(2) Improved functional JAG1 and NOTCH2 variant testing in patients with clinical or suspected Alagille syndrome using new low-Notch activity cellsHum GenetApril 18, 2026Nicole Buhl, Eva-Doreen Pfister, Daniel V. Oliveira, Fabio Turetti, Eberhard Lurz, Ulrich Baumann, Nataliya Di Donato, Thomas Illig, Britta Skawran, Emma R. Andersson, et al.glycosylation was analyzed by digestion with Peptide-N-glycosidase F (PNGase F) (New England Biolabs, #P0704S). Proteins were separated on an SDS-PAGE and transferred onto a PVDF or nitrocellulose membrane. Membranes

(3) Breast milk antibody Fc signatures track with HIV transmission during breastfeeding in ART-naïve mothersiScienceApril 17, 2026Nadege Nziza, Wonyeong Jung, Yunjoo Lee, Jeff C. Hsiao, Tina Chen, Fan Li, Caroline Atyeo, Douglas A. Lauffenburger, Louise Kuhn, Nicole H. Tobin, et al.,Thermo Scientific,CAT # PIA39257 IdeZ protease,New England Biolabs,CAT #P0770S PNGase F,New England Biolabs,CAT #P0704S "gp120 (ConC, HIV-1 consensus clade C)",Immune Technology,CAT # IT-001–003p "gp140 (clade C, isolate

(4) Enzymatically Controlled Release of Proteins and Peptides (ENCOREP): A Promising Alternative Secretion ApproachiScienceApril 17, 2026Daniel Ivanusic, Gregor Zipf, Josef Maier, Hubert Bernauer, Norbert Bannert,New England Biolabs,Cat# M0202 TEV Protease,New England Biolabs,Cat# P8112S PNGase F,New England Biolabs,Cat# P0704S PNGase F,Promega,Cat# V4831 Benzonase Nuclease,Millipore,Cat# 70746-10KU "Sambucus nigra lectin (SNA, EBL

(5) ADAM10-Mediated Proteolytic Remodelling of Signalling and Adhesion Proteins on Brain Cell-Derived Small Extracellular VesiclesJ Extracell BiolApril 17, 2026Christopher C. Reimann, Hermann C. Altmeppen, Tomas Koudelka, Michaela Schweizer, Andreas Tholey, Behnam Mohammadi, Julia Bär, Lesley Cheng, Markus Glatzel, Marina Mikhaylova, et al.glycans were removed from sEV and cell lysate samples using the PNGase F kit (New England Biolabs, Cat# P0704). Cell lysates and sEVs in RIPA buffer with PIs were thawed from −80°C, and 1.5 µg of protein was denatured

(6) Mouse TAPBPR shows functional similarity to human TAPBPR in shaping the MHC-I immunopeptidomeFront ImmunolApril 15, 2026Juliana Bernardi Aggio, Marcel Wacker, William Guo, Arwen F. Altenburg, Ida Hafstrand, Reem Satti, Alison McDowall, Aure Aflalo, Andreas Neerincx, Jack Welland, et al.deglycosylation N-linked oligosaccharides from 20 µg of recombinant soluble TAPBPR were removed by PNGase F (CAT: P0704S, New England Biolabs (UK) Ltd., Hitchin, Hertfordshire, UK) treatment in denaturing reaction conditions,

(7) OGT mediates O-GlcNAcylation of MEIS2 and affects palatal osteogenic developmentInt J Oral SciApril 6, 2026Zhongyin Zhang, Zerui Shan, Xinyu Chen, Yu Xia, Li Meng, Yuxin Zhang, Caihong Wu, Lichan Yuan, Junqing Madenaturing in glycoprotein denaturing buffer and digestion with Peptide N-Glycosidase (New England Biolabs, P0704S) for the removal of N-linked glycoproteins. After being centrifuged, the supernatants underwent 2-h

(8) Structural basis of GAIN domain autoproteolysis and cleavage-resistance in the adhesion G-protein coupled receptorsNat CommunApril 6, 2026Fabian Pohl, Florian Seufert, Yin Kwan Chung, Robin Schick, Björn Kieslich, Torsten Schöneberg, Tobias Langenhan, Peter W. Hildebrand, Norbert Sträterexamining N-glycosylation of receptors, 17 μl of the lysates was treated with 500 U PNGase F (NEB, cat# P0704S), 500 U Endo H (NEB, cat# P0702S) or distilled water (1 μl each), supplemented with 2 μl of their respective

(9) A self-complementary recombinant adeno-associated virus vector coding for an anchorless prion protein carrying the G127V mutation extends survival in a rodent prion disease modelbioRxivMarch 30, 2026Thomas Zerbes, Claire Verkuyl, Cunjie Zhang, Sophie Grunnesjoe, Shehab Eid, Hamza Arshad, Wenda Zhao, Zahra Nasser, Teaghan O’Shea, Ari Belotserkovsky, et al.TAGTAGGCCTGGGACTC. PNGase F digest To remove N-linked glycans by digestion with PNGase F (catalog number P0704S, New England Biolabs, Ipswich. MA, USA), we made use of supplier provided buffers. Specifically, equal

(10) Glutamine-dependent changes in fibroblast-derived extracellular matrix dictate cancer cell behaviorMatrix BiologyMarch 25, 2026Julien Guillard, Jessica Stradley, Kristof Turan, Marta R. Storl-Desmond, Shan Lin, Yi-Chia Huang, Kevin Muñoz Forti, Christopher R. Weber, Simon Schwӧrertemperature, then diluted to 2 M urea in 100 mM NH4HCO3. 2000 U/100 μL PNGase F (New England Biolabs, P0704S) was added and incubated for 2 hours at 37°C, followed by addition of 2 μg/100 μL Lys-C (Thermo, 90307)

(11) ONX-0914 Suppresses Hormone-Sensitive Prostate Cancer by Promoting O-GlcNAcylation-Mediated Stabilization of TCF7L1Oncology ResearchMarch 23, 2026Peng Xian, Zhenwei Feng, Haitao Yu, Hubin Yin, Haonan Chen, Tenglin Shi, Xilai Li, Chunlin Zhang, Xuesong Bai, Xin Gou, et al.denaturation buffer at 100°C for 10 min and treated with PNGase F (500 U per reaction, New England Biolabs, P0704S, Ipswich, MA, USA) at 37°C for 1 h to remove N-linked glycosylation. The treated samples were incubated

(12) Cancer cell-derived sialylated IgG interacting with Siglec-7/9/10 is a potential immunotherapeutic target in pancreatic cancerCell Rep MedMarch 17, 2026Shenghua Zhang, Ming Cui, Xinmei Huang, Xiaoyao Feng, Ruiling Xiao, Qijia Liu, Jialu Bai, Xianlin Han, Xiaoding Liu, Weiyan Xu, et al.100-18B B27,Gibco,Cat# 17504044 Collagenase IV,Solarbio,Cat# C8160 DNase I,Solarbio,Cat# D8070 PNGase F,NEB,Cat# P0704 "α 2-3,6,8 Neuraminidase",NEB,Cat# P0720 α 2-3 Neuraminidase,NEB,Cat# P0743 "Human Siglec-9, His

(13) Scope of Microbial Transglutaminase for Site-Specific and Oriented Immobilization of Native Antibodies from Various Host SpeciesLangmuirMarch 10, 2026Emily Beitello, Kwame Osei, Faith E. Breausche, Jon A. Friesen, Jeremy D. DriskellZedira (catalog #T300). A PNGase F deglycosylation kit was purchased from New England Biolabs (catalog #P0704L). The following custom peptides were purchased from AAPPTec: dansyl-KKCC–COOH and dansyl-KKKCCC–COOH.

(14) The APOL1 variant p.N264K is predicted to block ion flow by occluding a pore at the cell surfaceLife Sci AllianceMarch 10, 2026Verena Höffken, Lara Console, Niklas Nelde, Hermann Pavenstädt, Cesare Indiveri, Thomas WeideGlycosylation status was investigated via digestion of N-linked oligosaccharides in RIPA lysates with PNGase F (P0704L; New England Biolabs) according to the manufacturer’s instructions. Digested samples were analyzed by Western blot

(15) Disrupted O-GalNAc glycosylation as a mechanism and biomarker of SLC35A2 -associated epilepsybioRxivMarch 4, 2026Robert G. Mealer, James J. Anderson, Sheridan L. Smith, Brianna M. Masters, Samuel H. Barth, Kaitlyn D. J. Huizar, Sahibjot Sran, Hyojung Yoon, Amanda Ringland, Savannah J. Muron, et al.overnight following manufacturers protocols (NEB, Neuraminidase A #P0722L, O-Glycosidase #P0733L, PNGase F #P0704L, and α1–3,4 Fucosidase #P0769S; Chondroitinase ABC, Sigma #C2905–5UN). Following transfer to nitrocellulose

(16) Biochemical analysis of a TrkB receptor mutation that causes a developmental epileptic encephalopathyJournal of Biological ChemistryMarch 1, 2026Yang Zhong Huang, Cormic McNamara, James O. McNamaraand geneticin (11811-031) were purchased from Gibco. PNgase F was purchased from New England Biolabs (P0704). Additional reagents were obtained from Sigma, unless specified otherwise. The mammalian expression

(17) SEL1L-HRD1 ER-associated degradation facilitates prohormone convertase 2 maturation and glucagon production in islet α cellsNature CommunicationsFebruary 25, 2026Wenzhen Zhu, Linxiu Pan, Xianwei Cui, Anna Chiara Russo, Rohit Ray, Brent Pederson, Xiaoqiong Wei, Liangguang Leo Lin, Mauricio Torres, Hannah Hafner, et al.Sigma-Aldrich C7698), endoglycosidase H (New England Biolabs P0702S), or PNGase F (New England Biolabs P0704S) per manufacturer’s instructions. In siRNA treatment experiments, control αTC cells were transfected

(18) Structures of proteinase 3 and the CD177 receptor complex reveal a major autoantibody epitopeEMBO ReportsFebruary 17, 2026Céline Zheng-Gérard, Jana Joha, Maria Carrasquero, Kamel El Omari, Edward Lowe, Shirish Dubey, Simon J Draper, Yu-Chi Chang, Hsi-Hsien Lin, Alan D Salama, et al.2005). PNGase F deglycosylation assay Glycosylated proteins were incubated with PNGase F (NEB, Cat.#P0704S) in accordance with the protocol supplied with the enzyme. In total, ∼20 μg of protein were incubated

(19) Generation and characterization of human iPSC-derived NPC1 I1061T/I10161T i 3 Neurons as a model for NPC1 diseasebioRxivFebruary 17, 2026Shikha Salhotra, Niamh X. Cawley, Christian White, Insung Kang, Anika Prabhu, Cristin D. Davidson, Christopher A. Wassif, Forbes D. Porterenzymes. For both Endo H (New England Biolabs, Ipswich, MA, #P0702S) and PNGase F (New England Biolabs, #P0704S) treatment, 100 μg of total protein for each cell type was combined with 5 μl of 10X glycoprotein denaturing

(20) Alpha-methyl-para-tyrosine and amphetamine ameliorate hyperactivity in a novel mouse model of dopamine transporter deficiency syndromeEMBO Molecular MedicineFebruary 16, 2026Emma E Russo, Ameneh Rezayof, Conner Wallace, Erin Q Williams, Pieter Beerepoot, Marija Milenkovic, Maria Novalen, Aled Blundell, Tatiana V Lipina, Jason Locke, et al.digested with the glycosidases peptide N-glycosidase F (PNGase F) and EndoH (New England Biolabs, catalog #P0704S and #P0702S, respectively). Sample proteins were then separated by 10% SDS-PAGE and transferred to PVDF

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PNGase F

PNGase F from New England Biolabs

Description

Specifications/Features

New England Biolabs

Citations (626)

Reviews

PNGase F from New England Biolabs

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