AKTA pure™ Protein Purification System from Cytiva

Product Specs
ItemAKTA pure™ Protein Purification System
CompanyCytiva Life Sciences
Price Get Pricing Get Quote for this Product
Catalog Number29018224 / 29018226 / 29046665 / 29046694 / 29302479 / 29707636 / 29707638
QuantityEA
Flow Rate25 mL/min or 150 mL/min
Pressure Range0 to 20 MPa
Wavelength RangeVaries

Cytiva Life Sciences

(1) Digital seed amplification assay for TDP-43 aggregate quantification in CSFAlzheimers DementMay 5, 2026Ella Borberg, Zoe Swank, Tal Gilboa, Lily A. Wickland Shearer, Arek V. Melkonian, Justin C. Rolando, David R. Walt, Andrew M. SternpH 8.0). The target protein was further purified using size exclusion chromatography (SEC) with an AKTA Pure protein purification system on a HiPrep 16/60 Sephacryl S‐300 HR (Cytiva, #17116701) column with Tris‐buffered saline (TBS, 10 mM

(2) A practical guide for characterization of novel CRISPR-Cas systems with Pro-CRISPR factorsBiophysics ReportsApril 30, 2026Ao Sun, Shu-Lin Jin, Jun-Jie Gogo Liu(VWR, Cat. #490005-908) • JY99-IIDN Ultrasonic Homogenizer (SCIENTZ) • AKTA Pure 25M (Cytiva, Cat. #29018226) • AKTA Micro (Cytiva, Cat. #29302479) • JY-SCZ8 Vertical Electrophoresis Tank (JUNYI Electrophoresis

(3) Mechanistic design of cell-penetrating disruptors for phospho-dependent TACC3-CHC interactionStructureApril 27, 2026Vanda Gunning, Matthew Batchelor, Krista K. Alexander, Martin Walko, Taylor C. Dill, Selena G. Burgess, Stephen J. Royle, Eileen J. Kennedy, Richard Bayliss7.0.3.,Dotmatics,RRID:SCR_015052 BioRender,https://BioRender.com/2jqchpm,RRID:SCR_018361 Other,Other,Other Akta pure protein purification system,Cytiva,N/A 5 ml HisTrap HP column,Cytiva,Cat# 17524802 5 ml HiTrap Q HP column,Cytiva,Cat# 17115301 HiLoad

(4) RNA virus polymerase-helicase coupling enables rapid elongation through duplex RNACell ReportsApril 16, 2026Pim P.B. America, Subhas C. Bera, Arnab Das, Thomas K. Anderson, John C. Marecki, Flávia S. Papini, Jamie J. Arnold, Robert N. Kirchdoerfer, Craig E. Cameron, Kevin D. Raney, et al.study,https://doi.org/10.48338/VU01-DY3HI3 Other,Other,Other 3130XL Genetic Analyzer,Applied Biosystems,Cat # 106MI60-01 Akta Pure 25 M FPLC,Cytiva,29018226 Amicon Ultra-15 centrifugation filter,MilliporeSigma,Cat # UFC9010D Centrifugal concentrator,Vivaspin,Cat

(5) Antibacterial microcins self-assemble to promote biofilm formation in Acinetobacter baumanniiCell ReportsApril 1, 2026Mady S. Telford, Michelle Mikesh, Jennifer K. Parker, Robert L. Beckman, Robert W. Newberry, Renee M. Fleeman, Bryan W. Daviesadditional alanine was added to the N terminus of the purified microcin. Protein was isolated using the Akta Pure Protein Purification System (Cytiva). To eliminate MBP contamination, fractions were rotated with amylose for 1 h at 4°C. Protein

(6) Simultaneous inhibition of bacterial virulence and anti-phage defense systems by synergistic bacteriophage counter-defense proteinsThe EMBO JournalMarch 18, 2026Jingru Zhao, Yuhao Zhu, Chenchen Wang, Fan Tian, Jun Deng, Jianglin Liao, Zhuojun Zhong, Jiazhen Liu, Nannan Guo, Shuai Le, et al.V9,N/A,https://www.graphpad-prism.cn/ SnapGene software V8.0.3,N/A,https://www.snapgene.cn/ Other,Other,Other ÄKTA pure,GE Healthcare/Cytiv,29018224 Reagents and tools table

(7) Reduced glycoprotein hormone β-5 links male aging and testosterone decline to increased adiposityJournal of Clinical InvestigationMarch 16, 2026Gengmiao Xiao, Aijun Qian, Zhuo Gao, Tingting Dai, Hui Liang, Shuai Wang, Mulan Deng, Yunjing Yan, Xindan Zhang, Xuedi Zhang, et al.with CTP linker and His-tag. The recombinant GPHA2/GPHB5 (CGH) protein was purified from medium using AKTA pure Protein Purification System (GE Healthcare, now Cytiva). For desalting, we used a 3 kDa ultrafiltration tube (MilliporeSigma, UFC900396)

(8) The oncogenic CCDC6-RET fusion protein is a dual ATP- and ADP-dependent kinaseNature CommunicationsMarch 6, 2026Ana Martín-Hurtado, Julia Contreras, Jana Sánchez-Wandelmer, Eduardo Zarzuela, Fernando García, Johanne Le Coq, Jasminka Boskovic, Inés G. Muñoz, Federico Gago, Javier Muñoz, et al.measured by absorbance using a Nanodrop. When required, a size exclusion chromatography (SEC) step using AKTA pure protein purification system (GE Healthcare) was performed. The sample was concentrated up to 2 mL with a Millipore concentrator

(9) A gated hydrophobic funnel within BAX binds bioactive lipids to potentiate pro-apoptotic functionNature CommunicationsFebruary 25, 2026Jesse D. Gelles, Yiyang Chen, Mark P. A. Luna-Vargas, Ariele Viacava Follis, Md Abdullah Al Noman, Md Kabir, Stella G. Bayiokos, Jarvier N. Mohammed, Tara M. Sebastian, Ngoc Dung Pham, et al.(Cat. No. 29148721, Cytiva) equilibrated with Gel Filtration Buffer using an ÄKTA pure™ 25 L1 (Cat. No. 29018224, Cytiva) at 4 °C according to the manufacturer’s instructions. 0.5 ml fractions were collected and fractions

(10) On-Column Dual-Gradient Refolding for Efficient Recovery of Insoluble Affinity-Tagged Recombinant ProteinsBio ProtocFebruary 5, 2026Anna Vlaskina, Dmitry Petrenko, Yulia Agapova, Anastasia Kuzminkova, Marta Evteeva, Maxim Patrushev(Smart-Lifesciences, catalog number: SLM011) 11. Chromatography system ÄKTA Pure (Cytiva, catalog number: 29018226) 12. Magnetic stirrer Heidolph MR Hei-Standard (Heidolph, catalog number: 505-20000-00) 13. Vertical

(11) On-Column Dual-Gradient Refolding for Efficient Recovery of Insoluble Affinity-Tagged Recombinant ProteinsBIO-PROTOCOLFebruary 5, 2026Anna Vlaskina, Dmitry Petrenko, Yulia Agapova, Anastasia Kuzminkova, Marta Evteeva, Maxim Patrushev(Smart-Lifesciences, catalog number: SLM011) 11. Chromatography system ÄKTA Pure (Cytiva, catalog number: 29018226) 12. Magnetic stirrer Heidolph MR Hei-Standard (Heidolph, catalog number: 505-20000-00) 13. Vertical

(12) Reduced glycoprotein hormone β5 links male aging and testosterone decline to increased adiposityJ Clin InvestFebruary 3, 2026Gengmiao Xiao, Aijun Qian, Zhuo Gao, Tingting Dai, Hui Liang, Shuai Wang, Mulan Deng, Yunjing Yan, Xindan Zhang, Xuedi Zhang, et al.with CTP linker and His-tag. The recombinant GPHA2/GPHB5 (CGH) protein was purified from medium using AKTA pure Protein Purification System (GE Healthcare, now Cytiva). For desalting, we used a 3 kDa ultrafiltration tube (MilliporeSigma, UFC900396)

(13) Bora bridges Aurora-A activation and substrate recognition of PLK1EMBO ReportsJanuary 28, 2026Jennifer A Miles, Matthew Batchelor, Martin Walko, Vanda Gunning, Andrew J Wilson, Megan H Wright, Richard Baylisssoftware,https://www.thermofisher.com/uk/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-imaging-analysis/ibright-systems/software.html#ibright-analysis-software-secure, Other,Other,Other Hidex Sense plate reader,Hidex, Akta pure protein purification system,Cytiva, 5 ml HisTrap HP,Cytiva,Cat #17524802 Amicon Ultra Centrifuge Filters 10 kDa MWCO,Millipore,Cat

(14) Granzyme B-mimetic nanozyme for nanovesicle targeted anticancer applicationsNature CommunicationsJanuary 26, 2026Xueyan Hu, Qiqi Liu, Helong Kang, Ming Zhao, Zheng Zhou, Yuanke Li, Weicheng Peng, Tianyi Qi, Ruming Liu, Li Jiao, et al.10 min. The supernatant was concentrated with an Amicon-Ultra (30 kDa, Millipore) and purified with AKTA pure protein purification system (GE Healthcare) equipped with Superose 6 increase (GE Healthcare). The protein concentration was determined

(15) Argonaute-mediated RNA editing selectively repairs point mutationsNucleic Acids ResearchJanuary 6, 2026Zhiwei Zhang, Jinyue Wang, Tongyu Guo, Xiaolan Yu, Fei Wang, Hang Zhang, Yang Liu, Wenqiang Li, Yibin Cheng, Yanhong Peng, et al.through a 0.22-μm filter membrane and loaded onto a 5-ml Heparin HiTrap column (GE Life Sciences) by the AKTA pure protein purification system (Cytiva). The heparin column was washed with wash buffer A for 5 column volumes, then followed by a

(16) The solute-binding proteins DppA1-5 of Pseudomonas aeruginosa have distinct substrate profilesSci RepDecember 23, 2025Konstantin Plöchl, Thomas Böttchervolume 5 mL, Cytiva 28–9075−48) connected to an Äkta pure chromatograph (flow rate 5 mL/min, Cytiva 29018226) at 4 °C. After equilibration with 5 CV PBS, sample loading, and washing with 5 CV PBS, the target protein

(17) Targeting highly attenuated IL-18 to PD-1 for enhanced anti-tumor activityFront ImmunolDecember 18, 2025Xueyuan Zhou, Felix Klaus Geyer, Jeffrey Takimoto, Harald Kolmar, Brian RabinovichSuperdex 200 Increase column (Cytiva, Cat No. 17104302) using an ÄKTA pure system (Cytiva, Cat No. 29018226). Monomeric fractions were pooled and formulated in 20 mM sodium citrate, 50 mM NaCl, 3% mannitol, 20

(18) Evaluating the Antifungal Potential of Autophagy-Related Protein 4 (ATG4) Inhibitors against Human Fungal PathogensJ Microbiol BiotechnolDecember 15, 2025Seungmee Jung, Jongchan Woo, Hyunjin Cha, Seung-Heon Lee, Sagar Dahal, Yong-Sun Bahn, Eunsook Park(Millipore, USA). Concentrated CnAtg4T was gel filtrated using a Superdex 200 Increase 10/300 GL on an AKTA pure chromatography system (Cytiva, USA). In Vitro Cleavage Assay and Western Blot Chemical application for the in vitro cleavage assay

(19) De novo design of a two-step approach targeting Claudin-6 for enhanced drug delivery to solid tumorsJournal of Translational MedicineNovember 20, 2025Jiayao Yan, Liqing Zhong, Xiaotong Chen, Lin Li, Fangcen Liu, Lei Lei, Mengchao An, Xiao Wei, Ying Wang, Tianran Chen, et al.centrifugation, the supernatant was collected for purification via an AKTA HisTrap HP column and an AKTA pure protein purification system (Cytiva, Danaher, Uppsala, Sweden) according to the manufacturer’s instructions. After dialysis against

(20) KLF5 enables dichotomous lineage programs in pancreatic cancer via the AAA+ ATPase coactivators RUVBL1 and RUVBL2Nature CommunicationsNovember 15, 2025Patrick J. Cunniff, Nicole Sivetz, Damianos Skopelitis, Olaf Klingbeil, Daniel Toobian, Diogo Maia-Silva, Mikala Egeblad, Christopher R. VakocNanoDrop. A size exclusion purification step followed each purification using an AKTA® Pure 25 M (Cytiva 29018226) using a Superdex® 200 Increase 10/300 GL (for MBP, MBP-KLF5, and RUVBL2) or a Superose® 6 Increase 10/300 GL

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AKTA pure™ Chromatography System

AKTA pure™ Protein Purification System from Cytiva

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Cytiva Life Sciences

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AKTA pure™ Protein Purification System from Cytiva

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