AKTA start™ Protein Purification System from Cytiva

Product Specs
ItemAKTA start™ Protein Purification System
CompanyCytiva Life Sciences
Price Get Pricing Get Quote for this Product
Catalog Number29022094
QuantityEA
Electrical Requirements100 to 240 VAC , 50 to 60 Hz
Dimensions(WxDxH) 340 x 280 x 360 mm
Flow Rate0.5 to 5 ml/min (operating range), 10 ml/min (wash flow)
Wavelength(s)280 nm ± 3 nm, single wavelength (UV monitor)

Cytiva Life Sciences

(1) Protocol for large-scale, high-yield, high-purity extracellular vesicle purification from human plasmaSTAR ProtocMarch 20, 2026Saba Zanganeh, Gabriel F. Arias, Allaura S. Cone, Runjie Yuan, Dirk P. Dittmercartridges,Cytiva,UFP-750-C-H24LA ÄKTA™ flux tangential flow filtration system,Cytiva,29038437 ÄKTA start™ chromatography system,Cytiva,29022094 ZetaView® Multi-laser TWIN Nanoparticle Tracking Analyzer,Particle Metrix,PMX-230-Z-488/640 3000 Series

(2) STARD3 regulates lysosome positioning and contacts via a GSK3-controlled phosphorylation switchThe EMBO JournalFebruary 25, 2026Julie Eichler, Corinne Wendling, Sophie Huver, Mehdi Zouiouich, Victor Hanss, Anna Cardinal, Victoria Fimbel, Catherine Birck, Alastair G McEwen, Céline Knorr, et al.2025)", ZeroCostDL4Mic,"(von Chamier et al, 2021)", Other,Other,Other ÄKTA Start chromatography system,Cytiva,29022094 ÄKTA go chromatography system,Cytiva,29383015 NanoDrop 2000,Thermo Scientifics,ND-2000 Cell Disruptor

(3) Production of Novel Thermostable Esterases from Thermus thermophilus Strain ET-1 in Escherichia coli and Thermus thermophilus HB27 Using the Bifunctional Expression System pTGT-1 and Characterization of the Recombinant EnzymesInternational Journal of Molecular SciencesJanuary 29, 2026Bernardita Valenzuela, Mayra Cayo, Francisco Solís-Cornejo, María-Belen Reyes, Ignacia Palma, Elena Uribe, Pedro ZamoranoParameters Ces1-ET, Est1-ET and Plp1-ET were partially purified by means of NTA-Ni2+ affinity chromatography in the AKTA Start Protein Purification System (Cytiva Company, Marlborough, MA, USA). Proteins were eluted with 100–200 mM imidazole and subsequently

(4) A pyridoxal radical carboligase and imine reductase photobiocatalytic cascade for stereoselective synthesis of unnatural prolinesNature ChemistryJanuary 1, 2026Chen Zhang, Jun Zhou, Binh Khanh Mai, Zijun Qin, James Finnigan, Samantha Gittings, Peng Liu, Yang Yang6×His tag were purified with a Ni-NTA column (5 mL HisTrap HP, GE Healthcare, Piscataway, NJ) using an AKTA Start protein purification system (GE healthcare). Proteins were eluted on a linear gradient from Histrap buffer A (100 mM KPi, 25 mM

(5) Novel monoclonal antibodies for immunodetection of AmpC β-lactamasesPeerJOctober 2, 2025Karolina Bielskė, Martynas Simanavičius, Julie Nuttens, Julija Armalytė, Justas Dapkūnas, Lukas Valančauskas, Aurelija Žvirblienėdiluted with purification buffer. Purification was performed using an ÄKTA™ start chromatography system (29022094; Cytiva, Marlborough, MA, USA) and 1 mL HisTrap™ HP column (29051021; Cytiva, Marlborough, MA, USA)

(6) Antineoplastic activity of a novel tri-specific single chain antibody targeting the hERG1/β1 integrin complex and TRAIL receptorsMolecular Cancer TherapeuticsOctober 1, 2025Claudia Duranti, Jessica Iorio, Chiara Capitani, Tiziano Lottini, Michele Martinelli, Julia Roosz, Nicole Anderle, Tengku Ibrahim Maulana, Peter M. Loskill, Rossella Colasurdo, et al.were purified by affinity chromatography using an ÄKTA start protein purification system (Cytiva, cat. 29022094) with HisTrap HP 1 mL columns (Cytiva, cat. GE29-0485-86) at a running speed of 1 mL/minute. Elution

(7) Sensitive Detection of Nitrogen-Fixing Soil Bacteria by Impedimetric ImmunosensingACS OmegaSeptember 30, 2025Napolean Bonaparte Thanapaul, Shrijana Bista, Panlada Tittabutr, Vincent Blay, Piyanut Pinyou, Montarop Yamabhaimin. Purification of IgG was performed using a 1 mL HiTrap MabSelect Prism A column (Cytiva) on an AKTA start protein purification system. The purified antibody fraction was dialyzed against PBS (7 mL) using an Amicon Ultra Centrifugal device

(8) Sensitive Detection of Nitrogen-Fixing Soil Bacteria by Impedimetric ImmunosensingACS OmegaSeptember 30, 2025Napolean Bonaparte Thanapaul, Shrijana Bista, Panlada Tittabutr, Vincent Blay, Piyanut Pinyou, Montarop Yamabhaimin. Purification of IgG was performed using a 1 mL HiTrap MabSelect Prism A column (Cytiva) on an AKTA start protein purification system. The purified antibody fraction was dialyzed against PBS (7 mL) using an Amicon Ultra Centrifugal device

(9) Crosstalk between chromatin state and ATM signalling in DNA damage-induced transcription stressThe EMBO JournalAugust 26, 2025Irene Salas-Armenteros, Maarten Klunder, Wim Vermeulen, Maria TresiniMembrane,Millipore/Merck,Cat# IPVH00010 ÄKTA start,CYTIVA (https://www.cytivalifesciences.com/en/us),Cat# 29022094 Promega Glomax® Multimode reader,Promega,N/A LI-COR Odyssey CLx Imaging System,Li-COR,N/A Leica TCS

(10) Broadly reactive monoclonal antibodies against beta-lactamases for immunodetection of bacterial resistance to antibioticsSci RepMay 30, 2025Karolina Bielskė, Rasa Petraitytė-Burneikienė, Aliona Avižinienė, Justas Dapkūnas, Ieva Plikusienė, Silvija Juciutė, Miglė Stančiauskaitė, Aurelija Žvirblienė, Indrė Kučinskaitė-Kodzėpurification buffer I or II. Purification was carried out with an ÄKTA start chromatography system (Cytiva, 29022094). Proteins rDHA-1, rACT-14, rPDC-195, and rCMY-34 were purified using metal chelate affinity chromatography

(11) A streamlined tandem affinity purification of His-MBP-SpyCas9, without buffer exchange, suitable for in vitro cleavage applicationsMethodsXMay 12, 2025Filippo Fronza, Roberto Verardo, Claudio Schneidercentrifuge at ∼3′000x g for 1 h. Purification All chromatographic purification steps are done with an AKTA Start protein purification system (Cytiva, Danaher, Washington D.C.), or equivalent fast protein liquid chromatography (FPLC) system,

(12) A single amino acid mutation alters multiple neutralization epitopes in the respiratory syncytial virus fusion glycoproteinNpj VirusesApril 22, 2025Ahmed K. Oraby, Aleksandra Stojic, Farah Elawar, Leanne M. Bilawchuk, Ryley D. McClelland, Kaci Erwin, Madison J. Granoski, Cameron D. Griffiths, Justin D. Frederick, Elena Arutyunova, et al.DS-Cav1 protein via western blot. The protein was purified on an AKTAstart (Cytiva Life Science, Cat # 29022094) using a HisPur Ni-NTA chromatography cartridge (Thermo Fisher Scientific, Cat # 90099) and an isocratic

(13) Lasso peptides sviceucin and siamycin I exhibit anti-virulence activity and restore vancomycin effectiveness in vancomycin-resistant pathogensiScienceMarch 21, 2025Abdelhakim Boudrioua, Benjamin Baëtz, Solenn Desmadril, Christophe Goulard, Anne-Claire Groo, Carine Lombard, Sabrina Gueulle, Marie Marugan, Aurélie Malzert-Fréon, Axel Hartke, et al.120 sonic dismembrator,Fisher Scientific,Cat# 12337338 ÄKTA start chromatography system,Cytiva,Cat# 29022094 CFX96 Real-Time PCR system,BioRad,N/A POLARstar OMEGA,BMG Labtech,N/A

(14) The Pseudoenzyme β‐Amylase9 From Arabidopsis Activates α‐Amylase3: A Possible Mechanism to Promote Stress‐Induced Starch DegradationProteins: Structure, Function, and BioinformaticsJanuary 23, 2025Christopher E. Berndsen, Amanda R. Storm, Angelina M. Sardelli, Sheikh R. Hossain, Kristen R. Clermont, Luke M. McFather, Mafe A. Connor, Jonathan D. Monroe4°C. This supernatant was then diluted to 175 mL with Buffer A. Protein was then purified using an AKTA Start protein purification system with a 1 mL TALON cobalt‐affinity column using washes of 2%, 10%, 20%, and 100% Buffer B (Cytiva Life

(15) Evaluation of resazurin phenoxazine dye as a highly sensitive cell viability potency assay for natural killer cell‐derived extracellular vesicle‐based cancer biotherapeuticsJournal of Extracellular BiologyJuly 17, 2024Frederic St‐Denis‐Bissonnette, Shirley Qiu, Sarah E. Cummings, Melanie Kirkby, Yohannes Haile, Sarah Wassmer, Gauri Muradia, Jelica Mehic, Andrew Stalker, Amit Shrestha, et al.purification. SEC was performed on the AKTA Fast Protein Liquid Chromatograph (GE Lifesciences, cat#29022094) using the HiScale™ chromatography column 10/40 (Cytiva, cat#29360550) packed with CaptoCore 700 resin

(16) Capsid virus-like particle display improves recombinant influenza neuraminidase antigen stability and immunogenicity in miceiScienceJune 21, 2024Hyeog Kang, Mira Rakic Martinez, Kara-Lee Aves, Anna Kathrine Okholm, Hongquan Wan, Sylvie Chabot, Tahir Malik, Adam F. Sander, Robert Danielsversion 10,GraphPad Software,https://www.graphpad.com ,, Other,Other,Other ,, AKTA start,CYTIVA,Cat# 29022094 Bac-to-Bac™ Baculovirus Expression System,Thermo Fisher Scientific,Cat# 10359016 BioTek Cytation 5

(17) Optimizing Recombinant Cas9 Expression: Insights from E. coli BL21(DE3) Strains for Enhanced Protein Purification and Genome EditingBiomedicinesMay 31, 2024Agrawal S, Padmaswari MH, Stokes AL, Maxenberger D, Reese M, Khalil A, Nelson CE.(500 mL and 50 mL) (Eppendorf, Enfield, CT, USA)AKTA Start for protein purification (Cytiva, cat. no. 29022094)Oakridge Tubes (Thermofisher Scientific)Both 50 and 15 mL falcon tubes (Genessee Scientific, cat. no.

(18) Folding molecular origami from ribosomal RNAJournal of NanobiotechnologyMay 2, 2024Anastasia Shapiro, Noah Joseph, Nadav Mellul, Almogit Abu-Horowitz, Boaz Mizrahi, Ido Bacheletribosomal RNA subunits were purified by running the total RNA in the AKTA explorer (Cytiva AKTA Start 29022094) or by gel extraction. Total RNA wes run on 1 × TAE, 1% agarose gel (Bio-Lab 000171235900) in 1 × TAE

(19) Streamlining the Detection of Human Thyroid Receptor Ligand Interactions with XL1-Blue Cell-Free Protein Synthesis and Beta-Galactosidase Fusion Protein BiosensorsLife (Basel)September 27, 2023J. Porter Hunt, Tyler J. Free, Jackelyn Galiardi, Kevin M. Watt, David W. Wood, Bradley C. Bundywas expressed in E. coli BL21 DE3 STARTM cells with a pHT-T7RNAP-HIS plasmid and purified with the Akta Start Protein Purification System (GE Healthcare Bio-Sciences, Uppsala, Sweden) according to the manufacturer’s instructions. The purified

(20) Different SWI/SNF complexes coordinately promote R-loop- and RAD52-dependent transcription-coupled homologous recombinationNucleic Acids ResearchSeptember 22, 2023Carlota Davó-Martínez, Angela Helfricht, Cristina Ribeiro-Silva, Anja Raams, Maria Tresini, Sidrit Uruci, Wiggert A van Cappellen, Nitika Taneja, Jeroen A A Demmers, Alex Pines, et al.column chromatography, using a HiTrap™ MabSelect SuRe™ column (GE Healthcare, cat. 29-0491-04), on an AKTA START protein purification system equipped with an automated fraction collector (Cytiva), as recommended by the manufacturers. Eluted

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ÄKTA start™ Protein Purification System

AKTA start™ Protein Purification System from Cytiva

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Cytiva Life Sciences

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AKTA start™ Protein Purification System from Cytiva

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