M-MLV Reverse Transcriptase from Promega

Product Specs
ItemM-MLV Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM1705
Concentration200u/μl
Quantity50,000u

Specifications/Features

Unit Definition:
One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl (pH 8.3 at 25°C), 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H]dTTP, 0.025mM oligo(dT)50, 0.25mM poly(A)400 and 0.01% NP-40.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A distinct TCR+CD19+ population in the peritoneal cavity: B1a cells as precursors of extrathymic T cellsiScienceApril 17, 2026Su-Min Yee, Jeong-Eun Seon, Jae-Eun Seo, Hae-Jin Seo, Ja-Hun Seo, Seo-Hee Moon, Hyun-Su Koo, Ha-Rim Choi, Su-Man Kim, Hyung-Sik Kang,Sino Biological Inc.,Cat#50137-MNAE TRI Reagent®,MRC,Cat#TR 118 M-MLV Reverse Transcriptase,Promega,Cat#M1705 AccuPower® 2X GreenStar™ qPCR Master Mix,Bioneer,Cat#K-6253 PMA,Merck,Cat#P1585 Ionomycin,Merck,Cat#I0634 Protein Transport Inhibitor (containing Brefeldin A

(2) Modification of internal RNA domains from the long non-coding RNA XIST refines roles in silencing and heterochromatin recruitmentHum Mol GenetApril 2, 2026Maria Jose Navarro-Cobos, Bronwyn J Posynick, Christine Yang, Carolyn J Brownwas used to generate the cDNA by reverse transcription using M-MLV Reverse Transcriptase (Promega #M1705), with the following conditions for each 20 μL reaction: 4 μL of 5x buffer, 0.4 μL of 25 μM dNTPs, 1

(3) X-linked cancer-associated polypeptide (XCP) from lncRNA1456 modulates PHF8 histone demethylase activity to regulate the epigenome, gene expression, and cellular pathways in breast cancerOncogeneApril 1, 2026Shrikanth S. Gadad, Cristel V. Camacho, Xuan Gong, Micah Thornton, Venkat S. Malladi, Anusha Nagari, Aishwarya Sundaresan, Tulip Nandu, Sneh Koul, Yan Peng, et al.RNA was subjected to reverse transcription using Oligo-dT and MMLV reverse transcriptase (Promega, M1705). The resulting cDNA pool was treated with three units of RNase H (Enzymatics, Y9220L) for 30 min at

(4) Modeling Chronic BaP Exposure in Bronchial Epithelial Cells Reveals Multi-Scale Drivers of Early Preneoplastic ReprogrammingCellsMarch 22, 2026Cristian Andrade-Madrigal, Cecilia Rojas-Fuentes, Javier Díaz-Mijares, Gloria M. Calaf, Pablo M. Santoro, Alejandro H. Corvalán, Francisca J. Medina, Cristian G. Torres, Paula Romero-Vicencio, Julio C. Tapia, et al.DNA contamination. Complementary DNA (cDNA) synthesis was then performed using MMLV Reverse Transcriptase (M1705, Promega, Madison, WI, USA), utilizing oligo(dT) primers (C110A, Promega, Madison, WI, USA), dNTPs (U1515,

(5) The REDD1–NF-κB–miRNAs–eNOS/SIRT1 axis mediates obesity-induced endothelial cell senescence and hypertensionNature CommunicationsMarch 12, 2026Yoon Kyung Choi, Dong-Keon Lee, Minsik Park, Junyoung Byeon, Woo-Young Nam, Thuy Linh Lai, Kyu Nam Kim, Okhwa Kim, Sungwoo Ryoo, Jeong-Hyung Lee, et al.TRIzol reagent (#15596018, Invitrogen). First-strand cDNA was synthesized using M-MLV Reverse Transcriptase (#M1705; Promega), followed by qPCR for the quantification of target gene mRNA levels using target-specific

(6) FBXO39 knockdown promotes spermatogenesis impairment by inducing mitochondrial dysfunction and ferroptosis via inhibiting KDM5A ubiquitination and regulating H3K4me3 demethylationCell Biol ToxicolMarch 4, 2026Tao Li, Kun Wang, Yuxiang Chen, Zhuocheng Li, Shanda Li, Yu Zhang, Xuyuan Zhu, Haoran Shi, Liang Gao, Hongtao Jiangcytotoxicity. RT-qPCR Total RNA, extracted with TRIzol (Invitrogen, AM9738), was reverse-transcribed by a Promega kit (M1705). RT-qPCR amplification was carried out on an ABI 7500 system via GoTaq Master Mix (A6002, Promega).

(7) The conserved noncoding sequence CNS11 is a master control region for Rorc transcription in type 17 immune cellsScience AdvancesFebruary 27, 2026Hao Zhang, Dehui Chang, Shiyuan Xie, Ruifeng Li, Qian Zhang, Qi Xing, Tian Xie, Gongrui Zhang, Xinyi Lou, Shijie Li, et al.manufacturer’s protocol and reverse transcribed to cDNA using M-MLV Reverse Transcriptase (Promega, M1705). Relative gene expression was assessed using Hieff qPCR SYBR Green Master Mix (Yeasen, 11201ES03) with

(8) Step-by-Step Protocol for In Situ Profiling of RNA Subcellular Localization Using TATA-seqBIO-PROTOCOLFebruary 20, 2026Xiao Jiang, Chu Xu, Lulu HuT4 RNA ligase 2 truncated KQ (NEB, catalog number: M0373) 26. M-MLV reverse transcriptase (Promega, catalog number: M1705) 27. NEBNext Ultra II Q5 master mix (NEB, catalog number: M0544) 28. AMPure XP beads (Beckman, catalog

(9) PTEN-AKT2 Regulates Mixed Lineage Liver Cancer Development and Sensitizes Cancer Cells to TGFβ TreatmentResearch SquareJanuary 31, 2026Bangyan Stiles, Ielyzaveta Slarve, Ielyzaveta Slarve, Jingyu Chen, Yiwei Zeng, Yiwei Gu, Shunan He, Shunan Alhousari, Diala Alhousari, Brittney Hua, et al.#15596018). Reverse transcription was performed with the M-MLV reverse transcriptase system (Promega, PR-M1705). Quantitative PCR (qPCR) was then conducted using the APExBIO qPCR kit (#K1070). Gene-specific primers

(10) Long non-coding RNA UCA1 modulates SMARCA2-containing SWI/SNF chromatin remodeling complexes in human colorectal canceriScienceJanuary 16, 2026Bernadette Neve, Elsa Hadj Bachir, Belinda Duchêne, Mouloud Souidi, Martin Figeac, Jean-Pascal Meneboo, Emmanuelle Leteurtre, Christophe Mariette, Guillaume Piessen, François Corfiotti, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, M-MLV reverse transcriptase,Promega,Cat#M1705 random hexamer primers,Promega,Cat#C1181 DMEM,Thermo Fisher Scientific,Cat#41965-039 Fetal Bovine

(11) Increased caecal Intestinimonas abundance inhibits E. tenella gametogenesis via EtGFAT regulation and alleviates infection through immunityMicrobiomeJanuary 12, 2026Jun-Yi Li, Hai-Bin Huang, Chun-Wei Shi, Tian-Xu Pan, Ming-Han Li, Nan Wang, Jia-Jin Shan, Yan-Long Jiang, Wen-Tao Yang, Xin Cao, et al.(15,596,026; Invitrogen). Complementary DNA (cDNA) was synthesised using M-MLV reverse transcriptase (M1705; Promega) with random hexamer and oligo (dT)15 primers. qPCR amplification was performed using SYBR

(12) Modelling EWS::FLI1 protein fluctuations reveal determinants of tumor plasticity in Ewing sarcomaEMBO Molecular MedicineJanuary 3, 2026Veveeyan Suresh, Christoph Hafemeister, Andri Konstantinou, Sarah Grissenberger, Caterina Sturtzel, Martha M Zylka, Florencia Cidre-Aranaz, Andrea Wenninger-Weinzierl, Karla Queiroz, Dorota Kurek, et al.First-strand cDNA synthesis was performed using 1 µg of RNA, M-MLV Reverse Transcriptase (Promega, Cat#M1705), and Oligo(dT)15 primers (Promega, Cat#C1101). PCR amplification was performed using the resulting

(13) 3′UTR shortening alleviates miRNA repression of mRNAs critical for muscle stem cell differentiationThe EMBO JournalDecember 22, 2025Yi Zhu, Jianshu Wang, Deng Tong, Peixuan Jia, Suli Chen, Yangyang Li, Jiaying Fu, Qiming Li, Ping Hu, Yu Zhou, et al.15596018). cDNA synthesis was performed using random primers and M-MLV reverse transcriptase (Promega, M1705) or HiScript II 1st Strand cDNA Synthesis Kit (Vazyme, R212-01). For isoform-specific RT-qPCR, cDNA

(14) Baicalein Inhibits Proliferation, Migration, and Invasion of Mesothelioma Cells Through the p53-FOXM1 Signaling AxisCancer Manag ResDecember 19, 2025Xin-Yi Zheng, Chao Guo, Jie Peng, Ping-Xiang Tang, Ze Xia, Wen-Fei Xu, Xu-Hui Li(DP430). cDNA synthesis was conducted with an M-MLV (Moloney murine leukemia virus) system (Promega, M1705), utilizing a 1 μg portion of total RNA, and the resulting complementary DNA was subjected to qPCR.

(15) miR-338-3p targets WAPL to suppress proliferation and invasion in cervical cancer cells: experimental insightsWorld Journal of Surgical OncologyDecember 7, 2025Bo Li, Yuye Fu, Ruoyu Wang, Qian Wang, Li Wang, Wenkang Liuused for RNA extraction. Reverse transcription was performed using the Promega M-MLV kit (Cat. No. M1705). The lentiviral expression vector GV369/miR-338-3p, luciferase expression vector GV272, and viral packaging

(16) Decoding IFN-mediated immunity and cell dynamics in viral encephalitis: Insights from coxsackievirus B3 infectioniScienceDecember 3, 2025Lisa Marie Stach, Lisa Gerarda Maria Huis in ‘t Veld, Theres Schaub, Marina Jendrach, Marta Ornaghi, Marius Schwabenland, Antje Beling, Sandra Pinkert,Stemcell Technologies,Cat.#73782 murine Interferon-β,Sigma Aldrich,Cat.#I9032-1VL MLV Reverse Transcriptase,Promega,Cat.#M1705 random hexamer primers,Roche,Cat.#11034731001 pHrodo™ Red Zymosan BioParticles™,Thermo Fisher Scientific,Cat.#P35364 Cytochalasin D

(17) Long-term TCDD oral exposure changes gut microbial glycosylation patterns in female but not male C57BL/6 miceArchives of ToxicologyNovember 1, 2025Doreen Reichert, Stefan Janssen, Andrea Rossi, Charlotte Esserto the manufacturer’s instructions. cDNA synthesis was prepared with M-MLV Reverse Transcriptase (#M1705, Promega), RiboLock RNase Inhibitor (#EO0382, Thermo Fisher Scientific), and ROTH Hexanucleotide Random-Primer

(18) The designer cytokine IC7Fc attenuates atherosclerosis development by targeting hyperlipidemia in miceSci AdvOctober 24, 2025Wietse In Het Panhuis, Ellen Thiemann, Daisy M. A. H. van Dijk, Bibian Been, Kelsey E. Jarrett, Amber Meurs, Sander Kooijman, Milaine V. Hovingh, Melanie Modder, Bram W. van Os, et al.Ana, CA, USA). cDNA was subsequently synthesized from 1 μg of RNA using M-MLV Reverse Transcriptase (M1705, Promega, Madison, WI, USA) and quantitative polymerase chain reaction was performed using the SYBR

(19) citrOgen: a synthesis-free polysaccharide and protein antigen-presentation to antibody-induction platformNat CommunOctober 6, 2025Joshua L. C. Wong, Julia Sanchez-Garrido, Jaie Rattle, Jonathan Bradshaw, Vishwas Mishra, Gad FrankelcDNA was then synthesized using a Moloney murine leukemia virus (M- reverse transcriptase (M-MLV, #M1705) with random primers (#C1181) following the manufacturer´s instructions (all from Promega). To confirm

(20) The PP2A-B55α phosphatase is a master regulator of mitochondrial degradation and biogenesisSci AdvOctober 3, 2025Valentina Cianfanelli, Monica Nanni, Samantha Corrà, Sofia Mauri, David Sumpton, Sergio Lilla, Rossella De Cegli, Matteo Bordi, Giacomo Milletti, Caterina Ferraina, et al.was used for reverse transcription with M-MLV reverse transcriptase (Promega, WI, USA, catalog no. M1705) following the manufacturer’s instructions. The resulting cDNA was diluted to 5 ng/μl and stored at

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