M-MuLV Reverse Transcriptase from New England Biolabs

Product Specs
ItemM-MuLV Reverse Transcriptase
CompanyNew England Biolabs
Price $79.00 Inquire
Catalog NumberM0253S
Concentration200 u/µl
Quantity10000 units
ApplicationsDNA Analysis
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is the amount of enzyme required to incorporate 1 nmole of TTP into an acid insoluble form in 10 minutes at 37°C using poly(rA)-oligo(dT) as template primer.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) The Receptor for Advanced Glycation End-Products (RAGE) Regulates Cell Adhesion Through Upregulation of ITGA8CellsNovember 17, 2025Swetha Thiyagarajan, Estelle Leclerc, Stefan W. Vetterreverse transcription using Moloney Murine Leukemia Virus (M-MulV) Reverse Transcriptase (NEB cat# M0253, Ipswich, MA, USA) and an oligo(dT) primer. 2.2.5. Quantitative Real-Time PCR (qRT-PCR) A total of

(22) Cinchona-based liquid formulation exhibits antifungal activity through Tryptophan starvation and disruption of mitochondrial respiration in Rhizoctonia SolaniScientific ReportsNovember 7, 2025Simanta Das, Srinjoy Ghosh, Kumaresh Pal, Moumita Chakraborty, Surajit Khalko, Sk. Masudul Islam, Samuel Rai, Ashok Choudhury, Nandita Sahana, Somnath Mandalgene expression analysis, TRIzol™ reagent (Invitrogen, 15596026), M-MLV reverse transcriptase (New England Biolabs, M0253), dNTP mix (New England Biolabs, N0447), RNaseOUT™ inhibitor (Invitrogen, 10777019), SYBR™ Green Master

(23) Histone Deacetylase Inhibitors orchestrate epigenetic signalling and alter the nucleoporins and nuclear envelope in cervical cancerTranslational OncologyNovember 1, 2025Harsha Rani, Shabir Ahmad Ganai, Vijayalakshmi MahadevancDNA synthesis was performed using random hexamer primers (NEB #S1330S) and M-MuLV Reverse Transcriptase (NEB M0253S) as per manufacturer instructions. Real-time-PCR analysis was performed using Universal SYBR master

(24) Whitening fruit by CRISPR/Cas9-mediated homoeolog-specific gene editing of MYB10-1B in strawberry (F. × ananassa)Horticulture ResearchOctober 15, 2025Man Bo Lee, Yoon Jeong Jang, Hyeondae Han, Kanika Saxena, Youngjae Oh, Jae Yoon Kim, Seonghee LeeRNA was then reverse-transcribed to cDNA using oligo (dT) primers and M-MLV reverse transcriptase (M0253, New England Biolabs, MA, USA). To validate the gene expression associated with anthocyanin biosynthesis

(25) Transcriptional regulation of Ligase IV by an intronic regulatory element directs thymocyte developmentGenes & ImmunitySeptember 5, 2025Matthew D. Estrada, Christopher J. Gebhardt, Mariam A. Salem, Christina N. Rau, Kruthika Sharma, Rebecca A. Glynn, Craig H. Bassing, Eugene M. Oltz, Patrick L. Collinsreverse transcription (RT) control or processed into cDNA using M-MuLV Reverse Transcriptase (NEB, M0253S) with a poly A primer (IDT). Relative abundances of Beta-2 microglobulin (B2M) and Lig4 transcripts

(26) Simultaneous Capture of Chromatin-Associated RNA and Global RNA–RNA Interactions With Reduced Input RequirementsBIO-PROTOCOLSeptember 5, 2025Cheng Ding, Guoting Chen, Shiping Luan, Yuanyuan Gong, Cuilin Gui, Chen Yang, Zihe Xiang, Junjie Du, Mohamed F. Foda, Jiapei Yan, et al.(Thermo Fisher, catalog number: T2015) 44. M-MuLV reverse transcriptase (200 U/μL) (NEB, catalog number: M0253L) 45. M-MuLV reverse transcriptase reaction buffer (10×) (NEB, catalog number: B0253L) 46. RNase IF (NEB,

(27) C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerinElifeAugust 28, 2025Jessica Mella, Regan F Volk, Balyn W Zaro, Abigail BuchwalterBiolabs,E5520,High-efficiency DNA assembly Commercial assay or kit,MMLV reverse transcriptase,New England Biolabs,M0253,cDNA generation Commercial assay or kit,ZymoPURE II Midiprep Kit,Zymo Research,D4202,High-purity plasmid

(28) Epithelial-Mesenchymal Transition Activates YAP to Drive Malignant Progression and Immune EvasionCancers (Basel)August 25, 2025Xi Huang, Mingyan Zhang, Alexander D. Pearce, Matthew D. Gibbons, Dan Jin, Lu Li, Dongxin Hu, Renbin Liu, Mu Yu, Ming Tan, et al.total RNA using Moloney murine leukemia virus (M-MuLV) reverse transcriptase (NEB, Ipswich, MA, USA, M0253). Real-time PCR was then performed with PowerUp SYBR Green master mix (Applied Biosystems, Foster City,

(29) Thyme-synthesized silver nanoparticles mitigate immunosuppression, oxidative damage, and histopathological alterations induced by multidrug-resistant Enterococcus faecalis in Oreochromis niloticus: in vitro and in vivo assaysFish Physiol BiochemAugust 15, 2025Dalia A. Abdel-Moneam, Hanan S. Khalefa, Maha M. Rashad, Ghada E. Ali, Yasmine H. Ahmed, Eman Ragab, Osama A. Fouad, Ramadan A. Geioushy, Sahr B. Mahmoudpurity of RNA were confirmed, and then, RT-PCR was performed using M-MuLV Reverse Transcriptase (NEB #M0253). A fluorescence-based real-time detection method using the fluorescent SYBR green dye (Thermo Scientific,

(30) Tanshinone IIA suppresses fibro-adipogenic progenitor differentiation and attenuates fat infiltration in rotator cuff injury via Wnt/β-catenin pathwayRegen TherAugust 13, 2025Yumei Zhang, Xinghua Li, Rui Zhang, Minhui Wang, Tihui Wang, Guanfeng Liu, Amila Kuati, Wenhua MaoThe extracted RNA was then reverse transcribed into cDNA using NEB's reverse transcriptase (NEB, cat#M0253L). A reaction system of 40 μl containing 1∼0.5 μg RNA, 0.5 μM random primer, 0.5 μM oligo dT and 0.4

(31) Unravelling the Role of PIEZO1 in Adipogenesis of Fibrogenic/Adipogenic Progenitors for Muscle Fat Infiltration After Rotator Cuff TearJ Cachexia Sarcopenia MuscleJuly 29, 2025Xingzuan Lin, Qingfa Song, Amila Kuati, Zhanghua Li, Hao Zhou, Shujing Feng, Minhui Wang, Siyuan Zhu, Guoqing Cui, Jianhua Wang, et al.microgram of RNA was reverse transcribed into cDNA using MuLV reverse transcriptase (NEB, Cat. No. M0253L). The cDNA products were applied as templates of real‐time qPCR using Genious 2X SYBR Green Fast qPCR Mix

(32) Identification and Characterization of Static Craniofacial Defects in Pre-Metamorphic Xenopus laevis TadpolesJ Dev BiolJuly 25, 2025Emilie Jones, Jay Miguel Fonticella, Kelly A. McLaughlinM-MuLV Reverse Transcriptase according to New England Biolab’s first strand cDNA synthesis quick protocol (NEB #M0253, Ipswich, MA, USA) using d(T)23 VN oligo primers. Concentrations for both RNA and cDNA were assessed

(33) Knocking out ARL13B completely abolishes primary ciliogenesis in cell linesSci RepJuly 24, 2025Divyanshu Mahajan, Hui Min Chia, Lei Luand reverse-transcribed, primed by random nonamer primers using M-MuLV Reverse Transcriptase (NEB, #M0253), following the manufacturer’s standard protocol. The qPCR reactions were performed using the iTaq Universal SYBR Green Supermix

(34) Chronic ethanol administration exacerbates memory loss by altering N6-methyladenosine-mediated epigenetic signalingFront ImmunolJuly 1, 2025Yuanhang Liao, Fu Xu, Yuqing Yan, Sicheng Zhou, Na Liu, Baomin Dou, Nivetha Srinivasan, Weizheng Wang, Xiongwei Zhu, Jianghong Ye, et al.underwent reverse transcription using 6-nucleotide random primers and M-MLV reverse transcriptase (M0253, NEB). Quantitative PCR reactions were executed with SYBR™ Green Master Mix (43–856-12, Applied Biosystems)

(35) Functional analysis of CYP4B1 enzymes from apes and humans uncovers evolutionary hot spots for adaptations of the catalytical functionPLOS GeneticsJune 27, 2025Saskia Hüsken, Annika Röder, Johannes Ptok, Anne E. Meyer, Mats Georg, Yannick Schwarz, Christian Roos, Kerstin Mätz-Rensing, Michael C. Hutter, Doreen M. Floss, et al.manufacture’s protocol. cDNA was synthesized from 1 µg RNA using M-MuLV Reverse Transcriptase (NEB, #M0253L) with random hexamer primers. For amplification of CYP4B1 genes PCR assays were performed CYP4B1 specific

(36) Restoring endogenous Dlg4/PSD95 expression by an artificial transcription factor ameliorates cognitive and motor learning deficits in the R6/2 mouse model of Huntington's diseaseClin EpigeneticsJune 12, 2025Germán Fernández, Kevin Leiva, Fernando J. Bustos, Brigitte van Zundertperformed in a reaction mixture containing 2 µL RT buffer, 0.5 µL reverse transcriptase (NEB, Cat. No. M0253S), 0.5 µL RNase inhibitor (New England biolabs, Cat. No. M0307S), 0.5 µL dNTP mix, and 6.5 µL nuclease-free

(37) The Neurogenic Abnormities of Paraspinal Muscles Lead to Asymmetry of Fibre Types in Adolescent Idiopathic ScoliosisJ Cell Mol MedMay 24, 2025Tianyuan Zhang, Bin Li, Wenyuan Sui, Xiexiang Shao, Yaolong Deng, Zifang Zhang, Jingfan Yang, Zifang Huang, Sheng Li, Xin Fu, et al.according to the manufacturer's instructions and reverse transcribed by MuLV reverse transcriptase (NEB, #M0253L) at 42°C for 60 min. Quantitative PCR reactions were performed with FastStart Universal SYBR Green Master

(38) STAT3, MYC, and EBNA1 cooperate through a ZC3H18 transcriptional network to regulate survival and proliferation of EBV-positive lymphomasPLoS PathogMay 12, 2025Huanzhou Xu, Siva Koganti, Chenglong Li, Michael T. McIntosh, Sumita Bhaduri-McIntoshtranscriptase-quantitative PCR RT-qPCR was performed as described previously [57]. Briefly, MuLV Reverse Transcriptase (M0253L, New England Biolabs) was used for cDNA synthesis using 1 µg RNA as template. Quantitative PCR was then

(39) Ameliorative effects of date palm kernel extract against fenpropathrin induced male reproductive toxicityBiol ResMay 6, 2025Maher M. Soliman, Marsail S. Nashed, Eman I. Hassanen, Marwa Y. Issa, Abdelbary M. Prince, Ahmed M. Hussien, Adel F. Tohamyto the manufacturer’s instructions. cDNA was synthesized utilizing M-MuLV Reverse Transcriptase (NEB#M0253) according to the specified technique. The following primer sequences were used in the experiment for

(40) Leveraging the interconnected unfolded protein response and NLRP3 inflammasome pathways to reactivate Epstein-Barr virus in diffuse large B-cell lymphomasNAR CancerMay 5, 2025Huanzhou Xu, Tarun E Hutchinson, Siva Koganti, Beth A Rousseau, Daniel Xia, Michael T McIntosh, Sumita Bhaduri-McIntoshpreviously described [17]. Briefly, cDNA was synthesized using 1 μg of RNA and MuLV Reverse Transcriptase (M0253L, New England Biolabs). Quantitative PCR (qPCR) data were analyzed by normalizing to 18S rRNA using the

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