M-MuLV Reverse Transcriptase from New England Biolabs

Product Specs
ItemM-MuLV Reverse Transcriptase
CompanyNew England Biolabs
Price $316.00 Inquire
Catalog NumberM0253L
Concentration200 u/µl
Quantity50000 units
ApplicationsDNA Analysis
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is the amount of enzyme required to incorporate 1 nmole of TTP into an acid insoluble form in 10 minutes at 37°C using poly(rA)-oligo(dT) as template primer.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasisiScienceMarch 20, 2026Ke-En Tan, Zuag Paj Her, Cheng Zhang, Kok Siong Yeo, Taylor Weiskittel, Cassie Howe, Shyang Hong Tan, Katherine M. Johnson, Gina L. Razidlo, Thomas E. Rubino, et al.NucleoSpin® RNA kit, and random hexamer-converted cDNA was synthesized using M-MuLV reverse transcriptase (NEB, #M0253L) according to the manufacturer’s protocol. Quantitative reverse-transcription polymerase chain reaction

(2) Cell type-specific enhancers regulate IL-22 expression in innate and adaptive type 3 lymphoid cellsNature CommunicationsMarch 14, 2026Ankita Saini, Leone S. Hopkins, Vanida A. Serna, Matthew V. D. McCullen, Nicholas G. Selner, Bishan Bhattarai, José L. Fachi, Rebecca A. Glynn, Katharina E. Hayer, Craig H. Bassing, et al.protocol. The isolated RNA was then reverse transcribed to cDNA using M-MuLV Reverse Transcriptase (NEB, M0253) according to the manufacturer’s protocol. qPCR was performed using SYBR Green mastermix, and gene expression

(3) Evaluating the new product Norroa™ against Varroa destructor in managed honey bee (Apis mellifera) coloniesFront Insect SciMarch 11, 2026Devan Rawn, Cody Prouty, Asmita Gautam, Matthew Jamison, Win Talton, Katie Youngs, Ken Narva, Brian Manley, Cameron Jackconcentration, and equal amounts were used for cDNA synthesis. M-MuLV Reverse Transcriptase (NEB, Cat. No. M0253L) was used in combination with Oligo -d(T)20VN (IDT, Cat. No. 51-01-15-08) for cDNA synthesis. qPCR was

(4) Reduced Akr1b7 signaling drives ovarian aging and reproductive dysfunctioniScienceFebruary 20, 2026Keishiro Isayama, Kenji Watanabe, Masato Ohtsuka, Seisuke Kimura, Tomoaki Murata, Takeshi Honda, Masataka Asagiri, Shun Sato, Hiroshi Tamura, Norihiro Sugino, et al.#S1254S RNase inhibitor,Takara,Catalog # 2311A M-MuLV reverese transcriptase,New England Biolabs,Catalog #M0253S 0.1% poly-L-lysine solution in H2O,Sigma-Aldrich,Catalog #P8920 Paraformaldehyde,FUJIFILM Wako Pure

(5) JAK/STAT1-interferon-ISGylation networks in breast cancer resistance to inhibitors of FOXM1 and CDK4/6npj Breast CancerFebruary 12, 2026Yvonne Ziegler, Sandeep Kumar, Carlos M. Saeh, Blake N. Plotner, Grace O. Pento, Sung Hoon Kim, Alekya Raghavan, Rachel Schiff, John A. Katzenellenbogen, Benita S. Katzenellenbogen#15596018, Invitrogen, Carlsbad, CA, USA) and reverse transcribed using MMTV reverse transcriptase (cat. #M0253S, New England BioLabs, Ipswich, MA, USA, RRID:SCR_013517) or qScript Ultra Supermix (Cat. 95055-02 K,

(6) Therapeutic delivery of albumin-binding siRNA targeting IRS2 to diverse cell types reduces mammary tumor growthMolecular TherapyJanuary 29, 2026Claire E. Tocheny, Julianna E. Buchwald, Christopher D. Dahlke, Hassan H. Fakih, Jennifer S. Morgan, Ashley Summers, Christi A. Silva, Samuel O. Jackson, Ji-Sun Lee, Michael-Anthony Card, et al.reaction buffer (New England Biolabs; B0253S), and M-MuLV reverse transcriptase (New England Biolabs; M0253L) for cDNA synthesis. Synthesis of cDNA was performed using the following protocol: 16°C for 30 minutes

(7) Expression, transport, and storage of fetuin-B in human granulosa cellsScientific ReportsJanuary 23, 2026Bartosz Linek, Ann-Christin Meyer, Carmen Schoppe, Thorsten Enklaar, Dirk Prawitt, Walburgis Brenner, Konstantin Hofmannam Main, Germany), Oligo(dT)161028B008D04 (Metabion, Planegg, Germany), M-MuLV Reverse Transcriptase M0253L (New England BioLabs Inc., Frankfurt am Main, Germany), and deoxynucleotide solution mix (New England

(8) Butyrolactol A enhances caspofungin efficacy via flippase inhibition in drug-resistant fungiCellJanuary 22, 2026Xuefei Chen, H. Diessel Duan, Michael J. Hoy, Kalinka Koteva, Michaela Spitzer, Allison K. Guitor, Emily Puumala, Aline A. Fiebig, Guanggan Hu, Bonnie Yiu, et al.paper,N/A Cryptococcal FLAG-APT1-CDC50,This paper,N/A M-MuLV Reverse Transcriptase,New England Biolabs,M0253L M-MuLV Reverse Transcriptase Reaction Buffer,New England Biolabs,B0253S Difco™ Sabouraud Dextrose

(9) Transcriptome Sequencing and Differential Analysis of Testes of 1-Year-Old and 3-Year-Old Kazakh HorsesBiology (Basel)January 4, 2026Jiahao Liu, Yuting Yang, Liuxiang Wen, Mingyue Wen, Yaqi Zeng, Wanlu Ren, Xinkui Yaomin. First-strand cDNA was synthesized with random hexamer primers and M-MuLV Reverse Transcriptase (NEB #M0253), and second-strand cDNA was generated using DNA Polymerase I (NEB #M0209) and RNase H (NEB #M0297).

(10) Uncoupling Insulin Sensitivity From Longevity: A Sex‐Dependent Effect of Hepatic Glucagon SignalingAging CellJanuary 2, 2026Alexander Tate Lasher, Ben Heckman, Parth Sarker, Kaimao Liu, Liou Y. Sunwas reverse transcribed using M‐MuLV Reverse Transcriptase (New England Biolabs, Ipswich, MA catalog #M0253) following manufacturer recommendations. Quantitative PCR was carried out using a QuantStudio 3 system

(11) Fentanyl blockade of K + channels contributes to wooden chest syndromeThe Journal of PhysiologyJanuary 1, 2026Aguan D. Wei, Nicholas J. Burgraff, Luiz M. Oliveira, Thiago S. Moreira, Jan‐Marino Ramirezfollows. First-strand cDNA synthesis was achieved by standard reactions with M-MuLV reverse transcriptase (NEB cat. no. M0253S) using ~0.25 μg of total RNAs and random hexamer primers, including parallel control reactions without

(12) Protective Effects of Olea europaea L. Leaves and Equisetum arvense L. Extracts Against Testicular Toxicity Induced by Metronidazole Through Reducing Oxidative Stress and Regulating NBN, INSL-3, STAR, HSD-3β, and CYP11A1 Signaling PathwaysToxicsDecember 30, 2025Asmaa A. Azouz, Alaa M. Ali, Mohamed Shaalan, Maha M. Rashad, Manal R. Bakeer, Marwa Y. Issa, Sultan F. Kadasah, Abdulmajeed Fahad Alrefaei, Rehab A. AzouzNanodrop [46]. RT-PCR was performed using M-MuLV Reverse-Transcriptase (NEB, Ipswich, MA, USA, Cat.No.#M0253) [47]. qPCR was performed using SYBR green PCR Master Mix (Thermo Scientific, Waltham, MA, USA, Cat.

(13) DNA polymerase I is an efficient reverse transcriptase that mediates RNA-templated DNA repair synthesisbioRxivDecember 26, 2025Frances C. Lowder, Abigail H. Kendal, Lyle A. Simmonsbased on the 1X M-MuLV Reverse Transcriptase Reaction Buffer (New England Biolabs, B0253S). M-MuLV (M0253S) and E. coli Pol I (M0209S) controls were purchased from New England Biolabs. Commercial protein concentrations

(14) G-quadruplexes in the proximity 3′-UTR enhances alternative polyadenylation of Neogenin 1iScienceDecember 19, 2025Pauline Lejault, François Bolduc, Marc-Antoine Turcotte, Jean Pierre Perreault(NMM),"Frontier Scientific Inc., Logan, Utah",NMM580 MMuLV-reverse transcriptase,New England Biolabs,M0253S Q5 DNA polymerase,New England Biolabs,M0491S Restriction enzymes Xho I and BamH I,New England Biolabs,Lot

(15) The Cell Polarity Protein Scribble Is Involved in Maintaining the Structure of Neuromuscular Junctions, the Expression of Myosin Heavy Chain Genes, and Endocytic Recycling in Adult Skeletal Muscle FibersCellsDecember 16, 2025Lea Gessler, Yongzhi Jian, Nam Anh Ngo, Said Hashemolhosseiniwas synthesized with M-MuLV Reverse Transcriptase (New England Biolabs; Frankfurt am Main, Germany, M0253) following the manufacturer’s protocol. Quantitative PCR is performed using PowerUp SYBR Green Master Mix

(16) USPPAR is a cost-effective, scalable, and highly sensitive single-cell RNA sequencing workflow compatible with diverse specimensPLoS BiolDecember 15, 2025Ya-Wen Hsueh, Po-Min Chiang60%; ProteoGenix, 1114542); SUPERase·In (Thermo Fisher, AM2694); M-MuLV Reverse Transcriptase (NEB, M0253S); SuperScript II Reverse Transcriptase (Thermo Fisher, 18064014);); Maxima H Minus Reverse Transcriptase

(17) Molecular, Physiological, and Histopathological Insights into the Protective Role of Equisetum arvense and Olea europaea Extracts Against Metronidazole-Induced Pancreatic ToxicityLife (Basel)December 13, 2025Manal R. Bakeer, Maha M. Rashad, Asmaa A. Azouz, Rehab A. Azouz, Abdulmajeed Fahad Alrefaei, Sultan F. Kadasah, Mohamed Shaalan, Alaa M. Ali, Marwa Y. Issa, Salma I. El-Samanoudyvalidated using the nanodrop technique [18]. RT-PCR was carried out using M-MuLV Reverse Transcriptase (NEB#M0253). qPCR analysis was carried out using SYBR green PCR Master Mix (Thermo Scientific, Cat. No. K0221)

(18) Effectiveness of Multivitamins vs Folic Acid on Prevention of Neural Tube Defects in Mouse Genetic Models and Human OrganoidsAdvanced ScienceDecember 8, 2025Huili Li, Jing Zhang, Lori Bulwith, Lee Niswanderwas synthesized using Oligo d(T)23VN primer (NEB, #S1327) and First Strand cDNA Synthesis Kit (NEB, #M0253). Quantitative real‐time PCR was performed with the LightCycler 480 Real‐Time PCR System (Roche) against

(19) Analysis of Teg41 and PSMα promoter activity using a divergent fluorescent reporter plasmidmSphereNovember 25, 2025Paul Briaud, Julia R. Tennant, Ryan W. Steere, Richard E. Wiemels, Marvin Whiteley, Ronan K. Carrolland 25 µL for poly(U) reactions] as a template for reverse transcription using 1 µL of M-MulV RT (NEB M0253) and 1.45 µL of 3′ RACE adapter (10 mM) for poly(A) or 1.75 µL of 3′ RACE RT adapter (10 mM) for poly(U)

(20) Recombination between fragile regions associated with chromosomal rearrangements in glioblastoma can be mediated by RAGsiScienceNovember 21, 2025Amita Paranjape, Susmita Kumari, Lipsa Rani Sahu, Amrita Mondal, Swapna Kunhiraman, Arun Sharma M, Namrata M. Nilavar, Bibha Choudhary, Sathees C. RaghavanAcetate,Sigma,P1190 TRIS,Sigma,T1503 Leupeptin,Sigma,L9783 Pepstatin,Sigma,77170 Aprotinin,Sigma,A6279 PMSF,Sigma,P7626 Praformaldehyde,Sigma,158127 DABCO,Sigma,D27802 Trizol,Sigma,T9424 DNase,NEB,M0303 M-MuLV Reverse Transcriptase,NEB,M0253L SYBR Green PCR mix,Takara,4344463 RNAse A,Sigma,10109142001 PIPES,Sigma,P6757 Glycogen,ThermoFisher,AM9510 Ampicilin,Sigma,A61040 Chloramphenicol,Amresco,0230 Kanamycin,Sigma,K1377 Proteinase

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