MmeI from New England Biolabs

Product Specs
ItemMmeI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0637S
Quantity100 units
ApplicationsDNA Manipulation
Concentration2,000 U/ml
TypeRestriction Enzyme
Temperature-20 C

Sequence

TCCRAC(20/18)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) CRISPRi-seq in Haemophilus influenzae reveals genome-wide and medium-specific growth determinantsPLOS PathogensOctober 31, 2025Celia Gil-Campillo, Johann Mignolet, Asier Domínguez-San Pedro, Beatriz Rapún-Araiz, Axel B. Janssen, Vincent de Bakker, Jan-Willem Veening, Junkal Garmendia(New England BioLabs, R0107S) and RsrII (New England BioLabs, R0501S); (ii) MmeI (New England BioLabs, R0637S) and RsrII, for 1 h at 37ºC, and restriction enzymes were further inactivated for 20 min at 80ºC or

(2) ComK-induced cell death is reversed by upregulating the SigB or Spx pathway in Bacillus subtilisMicrobiology SpectrumSeptember 2, 2025Emma E. Wiesler, Qin Liao, Zhongqing Ren, Kathy F. Zhang, Jin Dai, Yinuo Ma, Gail G. Hardy, Xindan Wangusing QIAGEN DNeasy Blood & Tissue Kit (69504). Three micrograms of gDNA was digested with MmeI (NEB R0637S) for 90 min, then treated with quick calf intestinal alkaline phosphatase (CIP) (NEB M0525L) for 60

(3) Linear dicentric chromosomes in bacterial natural isolates reveal common constraints for replicon fusionmBioJune 11, 2025Ram Sanath-Kumar, Arafat Rahman, Zhongqing Ren, Ian P. Reynolds, Lauren Augusta, Clay Fuqua, Alexandra J. Weisberg, Xindan Wangusing QIAGEN DNeasy Blood and Tissue kit (69504). Three micrograms of gDNA was digested with MmeI (NEB R0637S) for 90 min and treated with calf intestinal alkaline phosphatase (Quick CIP, NEB M0525L) for 60 min

(4) Morphine Re-Arranges Chromatin Spatial Architecture of Primate Cortical NeuronsGenomics, Proteomics & BioinformaticsJune 1, 2023Liang Wang, Xiaojie Wang, Chunqi Liu, Wei Xu, Weihong Kuang, Qian Bu, Hongchun Li, Ying Zhao, Linhong Jiang, Yaxing Chen, et al.Hi-C DNA fragments after MmeI digestion Dissolved DNA was digested with 0.1 U/μl MmeI (Catalog No. R0637S, NEB) and 1.6 mM S-adenosyl-methionine (SAM; Catalog No. B9003S, NEB) at 37 °C for 1 h. Then, a native polyacrylamide gel electrophoresis

(5) A dicentric bacterial chromosome requires XerC/D site-specific recombinases for resolutionCurrent BiologyAugust 1, 2022Qin Liao, Zhongqing Ren, Emma E. Wiesler, Clay Fuqua, Xindan Wangisolation using QIAGEN DNeasy blood & tissue kit (69504). 3 μg of gDNA was digested with MmeI (NEB R0637S) for 90 min, and quick CIP (NEB M0525L) for 60 min at 37°C. The DNA was extracted using Phenol-Chloroform,

(6) Elucidation of the MicroRNA Transcriptome in Western Corn Rootworm Reveals Its Dynamic and Evolutionary ComplexityGenomics, Proteomics & BioinformaticsOctober 1, 2021Xiaozeng Yang, Elane Fishilevich, Marcelo A. German, Premchand Gandra, Robert E. McEwan, AndrÃ© Billion, Eileen Knorr, Andreas Vilcinskas, Kenneth E. Narvacleaved 20 bp downstream of the 3′-adapter with Mme1 type II restriction endonuclease (Catalog No. R0637S, New England Biolabs, Ipswich, MA) and ligated onto a double-stranded 3′-DNA adapter. Ligated fragments

(7) GRID-seq for comprehensive analysis of global RNA–chromatin interactionsNature ProtocolsJuly 1, 2019Bing Zhou, Xiao Li, Daji Luo, Do-Hwan Lim, Yu Zhou, Xiang-Dong FuMEM non-essential amino acid solution (100×; Gibco, cat. no. 11140076) MmeI (New England Biolabs, cat. no. R0637S) Dynabeads MyOne Streptavidin C1 (Thermo Fisher Scientific, cat. no. 65001) New England Biolabs

(8) High-salt–recovered sequences are associated with the active chromosomal compartment and with large ribonucleoprotein complexes including nuclear bodiesGenome ResearchNovember 1, 2018Marie-Odile Baudement et al.volume of 325 µL) are added, as well as 76 µL of water and 4 µL of MmeI restriction enzyme (NEB, ref. R0637S; final volume of 100 µL). This reaction is incubated 90 min at 37°C under agitation. The supernatant

(9) Decoding a Salmonella Typhi Regulatory Network that Controls Typhoid Toxin Expression within Human CellsCell Host & MicrobeJanuary 10, 2018Casey C. Fowler, Jorge E. Galán10236276001 Terminal Transferase,New England BioLabs,Cat # M0315S MmeI,New England BioLabs,Cat # R0637S Sau3AI,New England BioLabs,Cat # R0169S BamHI,New England BioLabs,Cat # R3136S ONPG,Sigma-Aldrich,Cat

(10) Genome‐Wide Fitness and Genetic Interactions Determined by Tn‐seq, a High‐Throughput Massively Parallel Sequencing Method for MicroorganismsCurrent Protocols in MicrobiologyFebruary 2, 2015Tim van Opijnen, David W. Lazinski, Andrew Camilli1E.3.1)1 mM Tris·Cl, pH 8.3 (APPENDIX 2A)2 U/μl MmeI restriction enzyme (New England Biolabs, cat. no. R0637S)32 mM S-adenosyl methionine (SAM; New England Biolabs)10× Buffer 4 (New England Biolabs)Calf intestinal

(11) Genome‐Wide Fitness and Genetic Interactions Determined by Tn‐seq, a High‐Throughput Massively Parallel Sequencing Method for MicroorganismsCurrent Protocols in Molecular BiologyApril 14, 2014Tim van Opijnen, David W. Lazinski, Andrew Camilli(Table 1) 1 mM Tris Cl, pH 8.3 (APPENDIX 2A) MmeI restriction enzyme (New England Biolabs, cat. no. R0637S) 32 mM S-adenosyl methionine (SAM; New England Biolabs) CutSmart buffer (New England Biolabs) Calf intestinal

(12) miRNA regulation in the early development of barley seedBMC Plant BiologyDecember 1, 2012Julien Curaba, Andrew Spriggs, Jen Taylor, Zhongyi Li, Chris Helliwell(5'-GTTCAGAGTTCTACAGTCCGAC-3') and P2 (5'-CGAGCACAGAATTAATACGACT-3'). Amplicons were digested by MmeI (New England Biolabs, #R0637S) and dephosphorylated by Shrimp Alkaline Phosphatase treatment (Roche #11758250001). Samples were run

(13) Fitness analyses of all possible point mutations for regions of genes in yeastNature ProtocolsJuly 1, 2012Ryan Hietpas, Benjamin Roscoe, Li Jiang, Daniel N. A. Bolon

(14) Identifying microbial fitness determinants by Insertion Sequencing (INSeq) using genome-wide transposon mutant librariesNature ProtocolsDecember 1, 2011Andrew L. Goodman, Meng Wu, Jeffrey I. GordonFragment (3’ - 5’ exo-; 5 U/μL) (New England Biolabs, Cat. No. M0212S)MmeI (New England Biolabs, Cat. No. R0637S)T4 DNA ligase (2,000,000 U/mL) (New England Biolabs, Cat. No. M0202T)Agarose (Roche, Cat. No. 11388991-001)Electrophoresis buffer

(15) Genome‐Wide Fitness and Genetic Interactions Determined by Tn‐seq, a High‐Throughput Massively Parallel Sequencing Method for MicroorganismsCurrent Protocols in MicrobiologyNovember 6, 2010Tim van Opijnen, Andrew Camilli(Table 1E.3.1) 1 mM Tris·Cl, pH 8.3 (APPENDIX 2A) MmeI restriction enzyme (New England Biolabs, cat. no. R0637S) HHMI Author Manuscript 32 mM S-adenosyl methionine (SAM; New England Biolabs) Buffer 4 (New England

(16) Chromatin Interaction Analysis Using Paired-End Tag SequencingCurrent Protocols in Molecular BiologyJanuary 1, 2010Melissa J. , Yuyuan , Chia-Lin , Xiaoan , Yijun

(17) Polony DNA sequencingCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]November 1, 2006Gregory J Porreca, Jay Shendure, George M Church

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4/3/2018Diagnostic Restriction Mapping Of Plasmid Constructs Triveni MahendrappaI have constructed plasmid for cloning purpose and it was 4010 bp in size. After transforming this plasmid into recipient E. coli cells, plasmid was prepped from few colonies and checked presence of ... Read Review

Chromatin Interaction Analysis Using Paired- End Tag Sequencing
January 01, 2010
Polony DNA Sequencing
October 01, 2006

MmeI

MmeI from New England Biolabs

Sequence

New England Biolabs

Citations (17)

Reviews

Protocols

Chromatin Interaction Analysis Using Paired- End Tag Sequencing

Polony DNA Sequencing

MmeI from New England Biolabs

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