hTERT RPE-1 from ATCC

Product Specs
ItemhTERT RPE-1
CompanyATCC
Price $8,500.00Supplier Page View Company Product Page
Catalog NumberCRL-4000
TypeCell Biology
SpeciesHomo sapiens
TissuePigmented epithelium:Retina, Eye
Cell LinehTERT RPE-1
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Retina
Cell Typeepithelial cell
DiseaseNormal

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(61) Comparative analysis of deep mutational scanning datasets in enteroviruses A and B identifies functional divergence and therapeutic targetsNature Ecology & EvolutionFebruary 23, 2026Beatriz Álvarez-Rodríguez, William Bakhache, Lauren McCormick, Ron Geller, Patrick T. Dolanpressures. Methods Cell lines and viruses Rhabdomyosarcoma (RD, CCL-136), HeLa-H1 (CRL-1958; RRID: CVCL_3334) and RPE (CRL-4000) cells were obtained from ATCC. All cell lines were periodically validated to be free of mycoplasma.

(62) CTCF/cohesin-binding sites are susceptible to replication-associated DNA damage and genomic instability in cancer cellsiScienceFebruary 20, 2026Elangoli Ebrahimkutty Faseela, Dimple Notani, Radhakrishnan Sabarinathan1% penicillin-streptomycin (Gibco, Invitrogen #15140163). hTERT RPE-1 cells (purchased from ATCC, #CRL-4000) were cultured in DMEM/F12 (Gibco, Thermofisher #10565042) with 10% FBS and 1% penicillin-streptomycin.

(63) The TRIP12’s intrinsically disordered region induces chromatin condensates and interferes with nuclear processesiScienceFebruary 20, 2026Claire Vargas, Alban Ricard, Damien Varry, Fernando Muzzopappa, Manon Brunet, Dorian Larrieu, Fabian Erdel, Guillaume Labrousse, Naïma Hanoun, Laetitia Ligat, et al.,Experimental models: Cell lines ,, HeLa S3,ATCC,CCL-2 HEK-293T,ATCC,CRL-3216 U2OS,ATCC,HTB-96 hTERT RPE-1,ATCC,CRL-4000 H2B-dsRed HeLa S3,Larrieu et al.15,N/A VHL-NbGFP4-FLAG HeLa S3,This study,N/A ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, Primers

(64) Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stabilityMolecular CellFebruary 19, 2026Carlos Origel Marmolejo, Celina Sanchez, Erin Helms, Melissa J. McEvoy, Juyoung Lee, Marcel Werner, Paige Roberts, Stephan Hamperl, Joshua C. SaldivarhTERT RPE-1 cells Cells obtained from an hTERT-immortalized retinal pigment epithelial cell line (ATCC, CRL-4000). RPE-1 cells were cultured in DMEM:F12 base medium supplemented with 10% fetal bovine serum (Thermo

(65) High-throughput ligand diversification to discover chemical inducers of proximityNature chemical biologyFebruary 16, 2026Shaum JB, Muñoz I Ordoño M, Steen EA, Wenge DV, Cheong H, Janowski J, Hunkeler M, Bilotta EM, Rutter ZJ, Barta PA, et al.University of Montreal) and EOL1 (DSMZ, ACC 386) cells were cultured in 10% FBS in RPMI 1640. RPE1 (ATCC, CRL-4000), HEK293T (ATCC, CRL-3216) and Lenti-X 293T (Takara Bio, 632180) cells were cultured in 10% FBS in DMEM.

(66) Acute NIPBL depletion reveals in vivo dynamics of loop extrusion and its role in transcription activationNature GeneticsFebruary 16, 2026Tessa M. Popay, Ami Pant, Femke Munting, Melodi Tastemel, Morgan E. Black, Nicholas Haghani, Jesse R. Dixonprograms through this organization. Methods Cell culture hTERT RPE-1 cells were obtained from ATCC (CRL-4000) and maintained in DMEM/F12 (Thermo Fisher #11330–057) supplemented with 10% FBS (Gibco #A5256701) and

(67) Chemical inhibition of a bacterial immune systemCell Host & MicrobeFebruary 11, 2026Zhiyu Zang, Olivia K. Duncan, Dziugas Sabonis, Iana Fedorova, Yun Shi, Gause Miraj, Shuai Le, Jun Deng, Yuhao Zhu, Yanyao Cai, et al.lines,Experimental models: Cell lines Human: immortalized retinal pigment epithelial cells (hTERT RPE-1),ATCC,Cat#CRL-4000,Cat#CRL-4000 Human: primary dermal fibroblasts normal; neonatal (HDFn),ATCC,Cat#PCS-201–010,Cat#PCS-201–010 Experimental models

(68) McIdas localizes to centrioles and controls centriole numbers through PLK4-dependent phosphorylationEMBO ReportsFebruary 5, 2026Marina Arbi, Margarita Skamnelou, Lydia Koufoudaki, Vasiliki Bakali, Spyridoula Bournaka, Sihem Zitouni, Stavroula Tsaridou, Ozge Karayel, Catherine G Vasilopoulou, Aikaterini C Tsika, et al.(Gibco) supplemented with 10% fetal bovine serum (FBS) in 5% CO2 at 37 °C. hTERT RPE-1 cells (ATCC, CRL-4000) were cultured in DMEM/F12 (Sigma-Aldrich) supplemented with 10% FBS under the same conditions. U2OS

(69) Transient proliferation by reversible YAP and mitogen control of the cyclin D1/p27 ratioCommunications BiologyJanuary 29, 2026Katherine R. Ferrick, Samsara W. Upadhya, Yilin Fan, Nalin Ratnayeke, Mary N. Teruel, Tobias MeyerCell culture Experiments were performed with human retinal pigmented epithelial cells (RPE1-hTERT immortalized, ATCC Cat# CRL-4000) unless specified otherwise. RPE1 were cultured in DMEM/F12 growth media with HEPES (Thermo Scientific

(70) PAF15–PCNA exhaustion governs the strand-specific control of DNA replicationNatureJanuary 28, 2026Gita Chhetri, Sugith Babu Badugu, Narcis-Adrian Petriman, Mikkel Bo Petersen, Aylin Seren Güller, Nora Fajri, Manon Coulée, Ganesha Pandian Pitchai, Jan Novotný, Frederik Tibert Larsen, et al.carcinoma cell line (CVCL_1922), primary immortalized retinal epithelial cell line hTERT-RPE1 (ATCC, CRL-4000), primary immortalized foreskin fibroblast BJ cells (ATCC, CRL-2522) and their derivatives were grown

(71) Proteomic profiling of UV damage repair patches uncovers histone chaperones with central functions in chromatin repairNature CommunicationsJanuary 28, 2026Alexandre Plessier, Audrey Chansard, Eliane Petit, Julia Novion Ducassou, Yohann Couté, Sophie E. Polostress. Methods Cell culture U2OS (ATCC HTB-96, human osteosarcoma, female), RPE-1 hTERT (ATCC CRL-4000, human retinal pigment epithelial cell, female), and NIH/3T3 cells (ATCC CRL-1658, mouse embryonic fibroblast,

(72) GMCL1 controls 53BP1 stability and modulates taxane sensitivityeLifeJanuary 19, 2026Yuki Kito, Tania J González-Robles, Sharon Kaisari, Juhee Pae, Sheena Faye Garcia, Juliana Ortiz-Pacheco, Beatrix Ueberheide, Antonio Marzio, Gergely Róna, Michele PaganoMycoplasma Detection Kit (Invivogen). HEK293T (ATCC CRL-3216), HeLa (ATCC CCL-2), and hTERT RPE-1 (ATCC CRL-4000) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco). U-2 OS (ATCC HTB-96), HCT-116

(73) GMCL1 controls 53BP1 stability and modulates taxane sensitivityElifeJanuary 19, 2026Yuki Kito, Tania J González-Robles, Sharon Kaisari, Juhee Pae, Sheena Faye Garcia, Juliana Ortiz-Pacheco, Beatrix Ueberheide, Antonio Marzio, Gergely Róna, Michele PaganoMycoplasma Detection Kit (Invivogen). HEK293T (ATCC CRL-3216), HeLa (ATCC CCL-2), and hTERT RPE-1 (ATCC CRL-4000) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco). U-2 OS (ATCC HTB-96), HCT-116

(74) Characterization of membrane structures regulating primary ciliogenesis by quantitative isotropic ultrastructure imagingbioRxivJanuary 16, 2026Quanlong Lu, Huijie Zhao, Ziam Khan, Adam Harned, Erina Kamiya, Valentin Magidson, Abhi Senthilkumar, Sumeth Perera, Kedar Narayan, Christopher J. Westlakeobtained from Sigma and used according to manufacturer’s instruction. Cell lines Human hTERT-RPE1 (CRL-4000) and 293T (CRL-3216) cell lines were obtained from ATCC. Human fibroblast cell line was obtained and

(75) Genetically encoded tool for manipulation of ATP/ADP ratio in human cellsbioRxivJanuary 16, 2026Alex E. Ekvik, Megan M. Kober, Denis V. Titovpolybrene and the mixture was used immediately. Cell generation and culture hTERT-RPE1 cells (ATCC #CRL-4000) were obtained from UC Berkeley Cell Culture Facility and maintained using DMEM/F12 (Gibco #12500062)

(76) 1-deoxysphingolipids dysregulate membrane properties and cargo trafficking in the early secretory pathwayCell Chemical BiologyJanuary 15, 2026Yi-Ting Tsai, Nicolas-Frédéric Lipp, Olivia Seidel, Riya Varma, Aurelie Laguerre, Kristina Solorio-Kirpichyan, Adrian M. Wong, Roberto J. Brea, Grace H. McGregor, Thekla Cordes, et al.Mito Stress Test,Agilent,Cat# 103015 Experimental models: Cell lines,, hTERT-RPE-1 cells,ATCC,ID# CRL-4000 RPE-1 SPTWT,This paper,N/A RPE-1 SPTC133W,This paper,N/A Recombinant DNA,, mCherry-Sec23A,Weigel

(77) Loss of ADAMTS9 disrupts ciliogenesis and collagen homeostasis resulting in Nephronophthisis-like polycystic kidneysbioRxivJanuary 6, 2026Sydney Fischer, Karyn L. Robert, Manu Ahmed, Griffin I. Kane, Matthew A. Kavanaugh, Wei Wang, Pamela V. Tran, Prabhani U. Atukorale, Sumeda Nandadasa3,3,5’-Triiodo-Thyronine 2 nM at 37°C, 5% CO2 as previously described [66]. Wild type HTERT-RPE-1 (ATCC, CRL-4000) and ADAMTS9 KO RPE-1 cells [15], were maintained in DMEM/F12 (Gibco;11330–032;) with 10% FBS and 200

(78) Single-cell RNA sequencing reveals a quiescence-senescence continuum and distinct senotypes following chemotherapyNature CommunicationsDecember 23, 2025Brianna Fernandez, Victor J. Passanisi, Humza M. Ashraf, Sabrina L. Spencerhydrocortisone, 100 ng/ml cholera toxin, and 100 μg/mL of penicillin and streptomycin. RPE-hTERT (ATCC CRL-4000) were obtained from ATCC and grown in DMEM/F12 supplemented with 10% FBS, 1x Glutamax, and 100 μg/mL

(79) Role of the SAF-A/HNRNPU ATPase and RGG domains in X chromosome inactivation, nuclear dynamics, transcription, splicing, and cell proliferationbioRxivDecember 20, 2025Judith A. Sharp, Rachael Thomas, Emily Sparago, Wei Wang, Michael D. Blowertreatment hTERT-immortalized RPE-1 cells were a gift from Brian Chadwick (Florida State University, Tallahassee, FL; ATCC CRL-4000) and were cultured as described [29]. The SAF-A degron cell line in RPE-1 cells has been described [29].

(80) A new model for coordinating the functions of TIMELESS at the replication forkbioRxivDecember 20, 2025Sameera Vipat, Rohan Harolikar, Karina Šapovalovaitė, Naga Raviteja Chavata, Syed Shahid Musvi, Arthur Morgunov, Sigvard Vällo, Tatiana N. Moiseevasupplemented with 10% FBS (GIBCO) and 1% penicillin-streptomycin (Invitrogen). RPE-1 hTERT cells (ATCC CRL-4000) were grown in DMEM/F12 medium (GIBCO) supplemented with 10% FBS (GIBCO) and 1% penicillin-streptomycin

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hTERT RPE-1 from ATCC

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