hTERT RPE-1 from ATCC

Product Specs
ItemhTERT RPE-1
CompanyATCC
Price $8,500.00Supplier Page View Company Product Page
Catalog NumberCRL-4000
TypeCell Biology
SpeciesHomo sapiens
TissuePigmented epithelium:Retina, Eye
Cell LinehTERT RPE-1
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Retina
Cell Typeepithelial cell
DiseaseNormal

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) The UVA-induced single-base mutational signature of CX-5461 in human cellsNucleic Acids ResAugust 25, 2026Elena Zaikova, Damian Yap, Armaghan Sarvar, Auden Hafezi, Jay Tan, Viviana Cerda, Kaylee Li, Daniel Lai, Karen Gelmon, John Hilton, et al.PowerPlex 16 HS kit), which showed that the transduced cells retained the parental RPE1 profile (ATCC CRL-4000) at all tested loci (Supplementary Table S1). Drugs CX-5461 for cell line experiments was purchased

(2) Synthesis and Biological Evaluation of a Series of N-Substituted Pyruvamide 4-Allylthiosemicarbazones and Their Copper(II) Complexes: Antibacterial, Antiradical, and Antiproliferative ActivitiesMoleculesAugust 16, 2026Ianina Graur, Dorin Istrati, Vasilii Graur, Victor Tsapkov, Olga Garbuz, Elena Melnic, Pavlina Bourosh, Jenny Roy, Aurelian GuleaCRL-1435), and HT-29 (ATCC: HTB-38), together with the normal epithelial cell line hTERT-RPE1 (ATCC: CRL-4000), were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). THP-1, PC-3, and

(3) Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screeningCell GenomicsAugust 12, 2026Jethro Langley, Lou Baudrier, Jada Curry, Kiran Narta, Hayley M. Todesco, Kyle Potts, Sorana Morrissy, Douglas J. Mahoney, Pierre Billontested for mycoplasma contamination. Lenti-X 293 T (Takara 632180), HEK293T (ATCC CRL-1573), RPE-1 (ATCC CRL-4000) and A549 (ATCC CCL-185) cells were cultured in DMEM (Corning 10-013-CV) supplemented with 10% Fetalgro

(4) TAK1 operates at the primary cilium in non-canonical TGFB/BMP signaling to control heart developmentPLoS BiolAugust 4, 2026Canan Doganli, Oskar Kaaber Thomsen, Daniel A. Baird, Yeasmeen Ali, Menachem V. K. Sarusie, Enrique Audain, Line Jeanett Jessen, Pauline Munck Truelsen, Johanne Bay Mogensen, Maria Schrøder Holm, et al.cells (laboratory stock; originally derived from the immortalized hTERT-RPE1 cell line, ATCC, clone CRL-4000) were cultured in T75 flasks at 37 °C and 5% CO2 in DMEM (Gibco) supplemented with 10% fetal bovine

(5) CDC7 and APC/CCdh1 gate distinct routes to initiate DNA replicationNature CommunicationsJuly 24, 2026Samsara W. Upadhya, Nalin Ratnayeke, Katherine R. Ferrick, David L. Rosenthal, Tobias MeyerCat# 25300054) and neutralized in DMEM/F12 supplemented with 20% horse serum. hTert-RPE-1 (ATCC Cat#CRL-4000, RRID:CVCL_4388) cells were cultured in DMEM/F12 with HEPES supplemented with 10% FBS (Sigma Cat# TMS-013-B).

(6) Isolation of the new polyketide (-)-R-talaropinophiloic acid guided by an integrated metabolomics-dereplication approachWorld J Microbiol BiotechnolJuly 23, 2026Marcus Vinicius Almeida Marques, Andresa Hiromi Sakai, Lucas Haidar Martorano, Viviani N. Takahashi, Juliana Mara Serpeloni, Fernando Martins Dos Santos Júnior, Jorge M. David, Eliane O. SilvaFor comparison, a normal retinal pigment epithelial cell line immortalized by hTERT (RPE-1) (ATCC® CRL-4000) was also assayed. T24 cells were cultured in Roswell Park Memorial Institute 1640 medium (RPMI 1640,

(7) Centrosome linker protein CEP68 modulates cellular stress responses via nuclear condensate formation and HSP27 interactioniScienceJuly 17, 2026Hui-Fang Hung, Peng-Peng Zhu, Craig BlackstoneRRID:CVCL_0045 HEK293 CRISPRed CEP68-FLAG,This paper,N/A HEK293 CRISPRed Halo-CEP68-FLAG,This paper,N/A RPE,ATCC,ATCC CRL-4000; RRID:CVCL_4388 Mouse primary neuron,The Jackson Laboratory,RRID:IMSR_JAX:000664 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, Guide

(8) hSpindly dynamics modulate spindle assembly checkpoint signaling, promoting resistance to mitotic inhibitorsiScienceJuly 17, 2026Mar Mora-Santos, Joaquín Herrero-Ruiz, Dirk Fennema-Galparsoro, Alejandro Belmonte-Fernández, Nuria Ferrandiz, Francisco RomeroExperimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, RPE-1,ATCC,Cat# CRL-4000; RRID: CVCL_4388 HCT116,ATCC,Cat# CCL-247; RRID: CVCL_0291 RPE-1 Flip-In T-Rex cells,Gift from Jonathon

(9) Cell-cycle-dependent regulation of DNA end resection by PLK1 and PLK3 without CtIP level modulationiScienceJuly 17, 2026Bing Pan, Fanghua Li, Emil Mladenov, Martin Stuschke, Beate Timmermann, George Iliakisa humidified atmosphere containing 5% CO2. Human retinal pigment epithelial RPE-1 hTERT cells (ATCC CRL-4000; female) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS

(10) Annexin A6 controls multi-organelle contact site formation and endolysosomal positioning, and remodels the STARD3 interactomeiScienceJuly 17, 2026Marc Bernaus-Esqué, Yangjing Liu, Eva Prats, Josep M. Estanyol, Gemma Martin, Maria Calvo, Panagiota Areti Gigourtsi, Mai Khanh Linh Nguyen, Alejandra R. Álvarez, Silvana Zanlungo, et al.transfections HeLa (kindly provided by Prof. G. Scita, IFOM, Italy), HEK293T (ATCC, CRL-3216) and hTERT RPE-1 (ATCC, CRL-4000) cell lines were grown in Dulbecco’s Modified Eagle’s Medium (DMEM, high glucose; Diagnovum D009-500)

(11) ESCRT-III assembles around mis-segregated DNA to protect genome stabilityNature Structural & Molecular BiologyJuly 15, 2026James Glover, Nathaniel Talledge, John McCullough, Akram Alian, Jessica B. A. Sadler, Sahil Kamboj, Nathaniel W. M. Dempsey, Thomas G. Laughlin, Henry C. Nguyen, Dawn M. Wenzel, et al.Cell culture Female HeLa (CRM-CCL-2, American Type Culture Collection (ATCC)), 293T (CRL-3216, ATCC) and hTERT RPE-1 (CRL-4000, ATCC) cells were cultured and maintained in DMEM (Gibco) supplemented with 10% FBS (Sigma-Aldrich)

(12) Soft matrix promotes ciliogenesis in human retinal pigment epithelial cellsSci RepJuly 13, 2026Rida Zahra, Natalie Munding, Katrin Domsch, Hannah Braun-Lee, Camila García-Navarrete, Motomu Tanaka, Gislene Pereiraused in this study were: telomerase immortalized human retinal pigment epithelial (RPE1) cells (ATCC, CRL-4000), RPE1 CEP83 KO (lab´s stock) and RPE1 cells stably expressing Tet3G or Tet3G ARL13B-GFP and γ-TUBULIN-mRuby218.

(13) CAD-C: An engineered nuclease enables repair-free in situ proximity ligation and nucleosome-resolution chromosome walks in human cellsbioRxivJuly 10, 2026Jan Soroczynski, Lauren Anderson Westcott, Wu Zuo, Arnold Ou, Hera Canaj, James Hickling, Joanna L. Yeung, Hide A. Konishi, Ellie B. Campbell, Conor Whelan, et al.described below to validate TEVp-dependent activation on nuclei. K562 (ATCC CCL243), hTERT RPE-1 (ATCC CRL-4000), and GM12878 (Coriell) cells were cultured under standard conditions and harvested at defined densities,

(14) Dynamic S-acylation controls CMG2 maturation extracellular matrix regulation and anthrax toxin susceptibility in vivoNature CommunicationsJuly 6, 2026Laurence Abrami, Octave Joliot, Sanja Blaskovic, Guillaume Valentin, Covadonga F. Hevia, Vincent Mercier, Béatrice Kunz, Francisco S. Mesquita, F. Gisou van der Gootnon-essential amino acids, penicillin, and streptomycin (GIBCO). Primary fibroblasts and RPE1 (ATCC CRL-4000) and RPE1 KO APT223 were grown in Dulbecco’s Modified Eagle Medium (Sigma Life Science) supplemented

(15) A viral ORFeome library for systems-level genetic dissection of host-pathogen interactionsCellJune 30, 2026Eric Fujimura, Colin N. O'Leary, Mamie Z. Li, Rachel A. Roberts, Caleb R. Glassman, Joao A. Paulo, Hanjie Jiang, Nouran S. Abdelfattah, Eric C. Wooten, Zachary Mirman, et al.RRID:CVCL_0063 A375,ATCC,Cat# CRL-1619; RRID:CVCL_0132 Immortalized HCEC,Roig et al.74,N/A hTERT-RPE1,ATCC,Cat# CRL-4000; RRID:CVCL_4388 THP-1,ATCC,Cat# TIB-202; RRID:CVCL_0006 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, See

(16) Bora, CEP192 and Cenexin regulate distinct Plk1-dependent cell and centrosome cycle transitionsNature CommunicationsJune 30, 2026Devashish Dwivedi, Crisálida Borges, Daniela Harry, Luca Cirillo, Patrick Meraldigenetically instable cancer cells. Methods Cell culture and drug treatments hTERT-RPE1 cells (ATCC: CRL-4000), hTERT-RPE1 Plk1-EGFP, hTERT-RPE1 USP28−/− (kind gift by Arshad Desai, University of California, San

(17) A microprotein encoded by FERMT3 modulates endothelial cell protein catabolism and induces cell cycle arrest and senescenceCell Commun SignalJune 25, 2026Manav Raheja, Beyza Güven, Witold Szymanski, Stefan Günther, Carsten Kuenne, Vladislav Rakultsev, Marta Segarra, Süleyman Bozkurt, Christian Münch, Manuel Kaulich, et al.AD-293 cells were cultured as described previously [69]. RPE-1 cells were originally obtained from ATCC (CRL-4000), passaged in DMEM/F12 media containing 10% serum, 1% pen/strep, and 0.01 mg/ml hygromycin B (Capricorn

(18) High-risk HPV E6 induces an aneuploidy-prone chromosome congression defect through destabilization of CENP-EJournal of VirologyJune 23, 2026Nanami Seshimo, Kanako Hori, Reiko Furuta, Toru Hirota, Ryu-Suke Nozawaopportunity. MATERIALS AND METHODS Cell culture, cell lines, and lentivirus hTERT-RPE1 cells (ATCC CRL-4000) were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum (FBS) and 10 mM HEPES (pH

(19) MCAK/Kif2C centromeric activity level tunes K-fiber turnover through distinct pathwaysiScienceJune 19, 2026Mike Wagenbach, Juan Jesus Vicente, Linda Wordemanparent,ATCC,CCL-247 Indian Muntjac (Muntiacus muntjak) parent,ATCC,CCL-157 htert-RPE (Homo sapiens) parent,ATCC,CRL-4000 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, "#2-CTGCGTTTCAGTGGAATGGAA, siRNA targeting

(20) Chemical inhibition of a CBASS anti-bacteriophage defenseiScienceJune 19, 2026Chengqian Zhang, Olga Rechkoblit, Shelley A.H. Dixon, Steven P. Angus, Joseph P. Gerdt(DE3),Ikujuni et al.42,N/A Human: immortalized retinal pigment epithelial cells (hTERT RPE-1),ATCC,CRL-4000 Human: primary dermal fibroblasts normal; neonatal (HDFn),ATCC,PCS-201-010 ,, Recombinant DNA,Recombinant

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hTERT RPE-1 from ATCC

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