hTERT RPE-1 from ATCC

Product Specs
ItemhTERT RPE-1
CompanyATCC
Price $8,500.00Supplier Page View Company Product Page
Catalog NumberCRL-4000
TypeCell Biology
SpeciesHomo sapiens
TissuePigmented epithelium:Retina, Eye
Cell LinehTERT RPE-1
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Retina
Cell Typeepithelial cell
DiseaseNormal

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) NDST3 suppression restores lysosomal acidification and ameliorates amyloid-β and MAPT/tau pathology in Alzheimer's diseaseTransl NeurodegenerApril 21, 2026Chuanhua Ge, Kun Wang, Huiyuan Tang, Yiling Ke, Huai Wang, Qiang Fu, Yun Xiu, Yongzheng Guo, Yun-Fang Jia, Zhimin Long, et al.Identifier: CSTR:19375.09.3101MOUSCSP5419) and human retina pigmented epithelial (RPE1) cells (ATCC CRL-4000) were cultured at 37 °C in a humidified incubator with 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM

(2) Novel ADPRS Missense Variant (p.Leu162Pro) Causes Stress-Induced Childhood-Onset Neurodegeneration With Ataxia and SeizuresNeurol GenetApril 17, 2026Priscilla Doria de Mattos, Rafael Dias de Moura, Murilo Fígaro Bertolino, Penélope Ferreira Valente, Isaac Araujo Matos, Vitor Marques Caldas, Paulo Emidio Lobao Cunha, Fernando Kok, Nicolas Carlos Hochfibroblasts were obtained from skin biopsies and grown in MEM (Thermo Fisher, MA). hTERT-RPE1 cells (ATCC: CRL-4000) were maintained in DMEM/F-12 (Thermo Fisher, MA) containing 15 mM HEPES (Merck, NJ). Media were also

(3) Melanocortin 3 Receptors Do Not Specifically Localize to Primary Cilia in Cultured Human and Rodent NeuronsCell Biochem FunctApril 16, 2026Niels Vos, Alessandro Moro, Wim Van Hul, Lotte Kleinendorst, Ralph J. Florijn, Susanne E. la Fleur, Matthijs Verhage, Mieke M. van Haelst, Ruud F. Toonenhypothalamic neurons, and NGN2‐induced human iNeurons. HEK293T (ATCC, CRL‐3216) and hTERT‐RPE1 cells (ATCC, CRL‐4000) were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco), penicillin–streptomycin,

(4) Nano-encapsulated Senecio glaucus L. essential oil with potent antioxidant, anticancer, and wound-healing propertiesRSC AdvApril 8, 2026Zainab H. Hussein, Sherif F. Hammad, Kuniyoshi Shimizu, Amr A. Nassrallah, Hesham S. M. SolimanCCL-247™), HepG-2 (ATCC® HB-8065™), MCF-7 (ATCC® HTB-22™), PC3 (ATCC® CRL-1435™), and RPE-1 (ATCC® CRL-4000™) cells using the MTT assay with slight modifications.42 Cells were seeded in 96-well plates at 1 ×

(5) ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell-Type Dependent and Tied to Ceramide HomeostasisFASEB JMarch 31, 2026Michal Mrkacek, Magda Tumova, Alex Puskasu, Pavol Utekal, Marek Vrbacky, Ladislav Kuchar, Petr Draber, Viktor Bugajevtranscriptase–immortalized retinal pigment epithelial cells (hTERT RPE‐1, hereafter referred to as RPE‐1; Cat# CRL‐4000; RRID: CVCL_4388) were obtained from ATCC, Manassas, VA, USA. RPE‐1 cells were maintained in DMEM of

(6) Protocol to enhance pre-sexual and sexual differentiation of Toxoplasma gondii using retinal cells and intestinal organoid-derived monolayersSTAR ProtocolsMarch 20, 2026Saira Cancela, Florencia Sena, Romina Pagotto, María E. Francia, Mariela Bollati-Fogolín(african green monkey kidney epithelial cells),ATCC,CCL-81 hRPE (human retinal pigmented epithelial),ATCC,CRL-4000 "L-WRN (murine fibroblast line producing Wnt-3A, R-spondin 3, and noggin-conditioned medium)",ATCC,CRL-3276 C57BL/6J

(7) Fructose-1,6-bisphosphate couples glycolytic activity to cell adhesionNature Cell BiologyMarch 16, 2026Lennart Hoffmann, Marlen Duchmann, Katina Lazarow, Yun-Hsuan Huang, Fabian Lukas, Wen-Ting Lo, Regina Feil, Christopher Schmied, Martin Lehmann, John E. Lunn, et al.female) and the telomerase-immortalized human retinal pigment epithelium cell line hTERT RPE-1 (ATTC: CRL-4000; female). Telomerase-immortalized HFF (male) were a kind gift of Professor Martin J. Humphries (University

(8) Tumour specific HORMAD1 expression perturbs mitotic arrest and drives sensitivity to mitotic kinase inhibitorsNat CommunMarch 11, 2026Callum Walker, Gabriel Kollarovic, Daniel Weekes, Jennifer Trendell, Ricarda M. Hoffmann, Alexia Martin, Riccardo Ferro, Blanca Navarro-Llinas, Luke Hitchen, Mercedes Pardo Calvo, et al.collected, filtered, and stored at −80 °C. Cell culture Cell lines obtained from ATCC: RPE1 hTERT (CRL-4000), HeLa (CCL-2), MCF10A (CRL-10317), MDA-MB-436 (HTB-130) NCI-H1299 (CRL-5803). Cell lines obtained from

(9) Comparative analysis of deep mutational scanning datasets in enteroviruses A and B identifies functional divergence and therapeutic targetsNature Ecology & EvolutionFebruary 23, 2026Beatriz Álvarez-Rodríguez, William Bakhache, Lauren McCormick, Ron Geller, Patrick T. Dolanpressures. Methods Cell lines and viruses Rhabdomyosarcoma (RD, CCL-136), HeLa-H1 (CRL-1958; RRID: CVCL_3334) and RPE (CRL-4000) cells were obtained from ATCC. All cell lines were periodically validated to be free of mycoplasma.

(10) The TRIP12’s intrinsically disordered region induces chromatin condensates and interferes with nuclear processesiScienceFebruary 20, 2026Claire Vargas, Alban Ricard, Damien Varry, Fernando Muzzopappa, Manon Brunet, Dorian Larrieu, Fabian Erdel, Guillaume Labrousse, Naïma Hanoun, Laetitia Ligat, et al.,Experimental models: Cell lines ,, HeLa S3,ATCC,CCL-2 HEK-293T,ATCC,CRL-3216 U2OS,ATCC,HTB-96 hTERT RPE-1,ATCC,CRL-4000 H2B-dsRed HeLa S3,Larrieu et al.15,N/A VHL-NbGFP4-FLAG HeLa S3,This study,N/A ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, Primers

(11) CTCF/cohesin-binding sites are susceptible to replication-associated DNA damage and genomic instability in cancer cellsiScienceFebruary 20, 2026Elangoli Ebrahimkutty Faseela, Dimple Notani, Radhakrishnan Sabarinathan1% penicillin-streptomycin (Gibco, Invitrogen #15140163). hTERT RPE-1 cells (purchased from ATCC, #CRL-4000) were cultured in DMEM/F12 (Gibco, Thermofisher #10565042) with 10% FBS and 1% penicillin-streptomycin.

(12) Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stabilityMolecular CellFebruary 19, 2026Carlos Origel Marmolejo, Celina Sanchez, Erin Helms, Melissa J. McEvoy, Juyoung Lee, Marcel Werner, Paige Roberts, Stephan Hamperl, Joshua C. SaldivarhTERT RPE-1 cells Cells obtained from an hTERT-immortalized retinal pigment epithelial cell line (ATCC, CRL-4000). RPE-1 cells were cultured in DMEM:F12 base medium supplemented with 10% fetal bovine serum (Thermo

(13) Acute NIPBL depletion reveals in vivo dynamics of loop extrusion and its role in transcription activationNature GeneticsFebruary 16, 2026Tessa M. Popay, Ami Pant, Femke Munting, Melodi Tastemel, Morgan E. Black, Nicholas Haghani, Jesse R. Dixonprograms through this organization. Methods Cell culture hTERT RPE-1 cells were obtained from ATCC (CRL-4000) and maintained in DMEM/F12 (Thermo Fisher #11330–057) supplemented with 10% FBS (Gibco #A5256701) and

(14) Chemical inhibition of a bacterial immune systemCell Host & MicrobeFebruary 11, 2026Zhiyu Zang, Olivia K. Duncan, Dziugas Sabonis, Iana Fedorova, Yun Shi, Gause Miraj, Shuai Le, Jun Deng, Yuhao Zhu, Yanyao Cai, et al.lines,Experimental models: Cell lines Human: immortalized retinal pigment epithelial cells (hTERT RPE-1),ATCC,Cat#CRL-4000,Cat#CRL-4000 Human: primary dermal fibroblasts normal; neonatal (HDFn),ATCC,Cat#PCS-201–010,Cat#PCS-201–010 Experimental models

(15) McIdas localizes to centrioles and controls centriole numbers through PLK4-dependent phosphorylationEMBO ReportsFebruary 5, 2026Marina Arbi, Margarita Skamnelou, Lydia Koufoudaki, Vasiliki Bakali, Spyridoula Bournaka, Sihem Zitouni, Stavroula Tsaridou, Ozge Karayel, Catherine G Vasilopoulou, Aikaterini C Tsika, et al.(Gibco) supplemented with 10% fetal bovine serum (FBS) in 5% CO2 at 37 °C. hTERT RPE-1 cells (ATCC, CRL-4000) were cultured in DMEM/F12 (Sigma-Aldrich) supplemented with 10% FBS under the same conditions. U2OS

(16) Transient proliferation by reversible YAP and mitogen control of the cyclin D1/p27 ratioCommunications BiologyJanuary 29, 2026Katherine R. Ferrick, Samsara W. Upadhya, Yilin Fan, Nalin Ratnayeke, Mary N. Teruel, Tobias MeyerCell culture Experiments were performed with human retinal pigmented epithelial cells (RPE1-hTERT immortalized, ATCC Cat# CRL-4000) unless specified otherwise. RPE1 were cultured in DMEM/F12 growth media with HEPES (Thermo Scientific

(17) PAF15–PCNA exhaustion governs the strand-specific control of DNA replicationNatureJanuary 28, 2026Gita Chhetri, Sugith Babu Badugu, Narcis-Adrian Petriman, Mikkel Bo Petersen, Aylin Seren Güller, Nora Fajri, Manon Coulée, Ganesha Pandian Pitchai, Jan Novotný, Frederik Tibert Larsen, et al.carcinoma cell line (CVCL_1922), primary immortalized retinal epithelial cell line hTERT-RPE1 (ATCC, CRL-4000), primary immortalized foreskin fibroblast BJ cells (ATCC, CRL-2522) and their derivatives were grown

(18) Proteomic profiling of UV damage repair patches uncovers histone chaperones with central functions in chromatin repairNature CommunicationsJanuary 28, 2026Alexandre Plessier, Audrey Chansard, Eliane Petit, Julia Novion Ducassou, Yohann Couté, Sophie E. Polostress. Methods Cell culture U2OS (ATCC HTB-96, human osteosarcoma, female), RPE-1 hTERT (ATCC CRL-4000, human retinal pigment epithelial cell, female), and NIH/3T3 cells (ATCC CRL-1658, mouse embryonic fibroblast,

(19) GMCL1 controls 53BP1 stability and modulates taxane sensitivityeLifeJanuary 19, 2026Yuki Kito, Tania J González-Robles, Sharon Kaisari, Juhee Pae, Sheena Faye Garcia, Juliana Ortiz-Pacheco, Beatrix Ueberheide, Antonio Marzio, Gergely Róna, Michele PaganoMycoplasma Detection Kit (Invivogen). HEK293T (ATCC CRL-3216), HeLa (ATCC CCL-2), and hTERT RPE-1 (ATCC CRL-4000) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco). U-2 OS (ATCC HTB-96), HCT-116

(20) 1-deoxysphingolipids dysregulate membrane properties and cargo trafficking in the early secretory pathwayCell Chemical BiologyJanuary 15, 2026Yi-Ting Tsai, Nicolas-Frédéric Lipp, Olivia Seidel, Riya Varma, Aurelie Laguerre, Kristina Solorio-Kirpichyan, Adrian M. Wong, Roberto J. Brea, Grace H. McGregor, Thekla Cordes, et al.Mito Stress Test,Agilent,Cat# 103015 Experimental models: Cell lines,, hTERT-RPE-1 cells,ATCC,ID# CRL-4000 RPE-1 SPTWT,This paper,N/A RPE-1 SPTC133W,This paper,N/A Recombinant DNA,, mCherry-Sec23A,Weigel

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hTERT RPE-1 from ATCC

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