Agilent BioTek ELx405 Select Deep Well Washer from Agilent Technologies

Product Specs
ItemAgilent BioTek ELx405 Select Deep Well Washer
CompanyAgilent Technologies
Price Get Pricing Get Quote for this Product
Catalog NumberELx405 Select
QuantityEA
ApplicationsELISA, Cell-based assays (model dependent), Magnetic bead, polystyrene bead, Multiplex assays, Bead-based ELISA
Electrical Requirements100 - 240 Volts AC. 50/60 Hz
Plate Formats96- and 384-well Low profile and standard height Deep well plates up to 50 mm
Manifold Channels96-tube Dual-Action manifold for 96- & 384-well washing
Residual Volume2 µL/well
Wash Volume50 to 3000 µL/well, in 1 µL increments
CapacityDispensing Liquids: 4 Wash buffers
Dimensions(WxDxH) 35.6 x 43.2 x 27.9 cm (14 x 17 x 11 in)
TypePlate washer
SoftwareOnboard programming, LHC™ pc software optional

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Agilent Technologies

5301 Stevens Creek Blvd

Santa Clara, California 95051

United States

Phone: +1 877-424-4536

Company Profile

Website: www.agilent.com

(1) IgM Antibodies Targeting Malondialdehyde Promote Complement‐Mediated Liver Injury in Alcohol‐Related Liver DiseaseLiver InternationalOctober 1, 2025Dragana Rajcic, Beatriz Pereira da Silva, Taras Baranovskyi, Benedikt Simbrunner, Benedikt S. Hofer, Constanze Hoebinger, Tereza Duckova, Nikolina Papac‐Milicevic, Stephan Listabarth, Sabine Weber, et al.water) and a Synergy 2 Luminometer (BioTek, Winooski, Vermont, USA). Washing steps were performed on an ELx405 Select Deep Well Microplate Washer (BioTek, Winooski, Vermont, USA) with PBS or PBS‐EDTA. Internal controls

(2) Isolation and Characterization of Antibodies Against Vascular Cell Adhesion Molecule-1 Reveals Putative Role for Ig-like Domains 2 and 3 in Cell-to-Cell InteractionInternational Journal of Molecular SciencesDecember 20, 2024Binura Perera, Yuao Wu, Jessica R. Pickett, Nadya Panagides, Francisca M. Barretto, Christian Fercher, David P. Sester, Martina L. Jones, Hang T. Ta, Lucia F. Zacchiwashed six times with PBS + 0.1% Tween 20 using a plate washer (BioTek, Agilent, Santa Clara, CA, USA, ELx405 Select). Next, a 1:5000 dilution of mouse anti-M13 monoclonal antibody (Sino Biological Cat# 11973-MM05T, RRID:AB_2857926)

(3) Identification of small molecule antagonists of sonic hedgehog/heparin binding with activity in hedgehog functional assaysBiochimica et Biophysica Acta (BBA) - General SubjectsNovember 1, 2024David R. Lamson, Michael Tarpley, Kezia Addo, Xiaojia Ji, Dina Abu Abu Rabe, Ben Ehe, Mark Hughes, Ginger R. Smith, Laura R. Daye, David L. Musso, et al.Additions were carried out using a NanoScreen NSX-1536 (NanoScreen, Hanahan, SC) and washes using a Biotek ELx405 Select CW microplate washer (Biotek Instruments, Winooski, VT). Briefly, ShhN was added to 384-well (flat-bottom,

(4) Re-assessment of monoclonal antibodies against diclofenac for their application in the analysis of environmental waters†Analytical MethodsMay 30, 2024Stephan Schmidt, Holger Hoffmann, Leif-Alexander Garbe, Andrea Harrer, Markus Steiner, Martin Himly, Rudolf J. Schneider(SpectraMax® Plus 384, Molecular Devices, Biberach an der Riss, Germany), an automatic washer (BioTek ELx405 Select™, Bad Friedrichshall, Germany) and a plate shaker (Titramax 100, Heidolph, Schwabach, Germany). ELISA:

(5) In-house ELISA protocols for capsid p24 detection of diverse HIV isolatesVirology JournalNovember 17, 2023Mariano A. Molina, Monique Vink, Ben Berkhout, Elena Herrera-Carrillosubstrates (see Table 1). We also evaluated the performance of our equipment, specifically the BioTek ELx405 Select washer and the GloMax® plate reader. During this process, we determined the ideal working volume for

(6) Mapping antibody footprints using binding profilesCell Reports MethodsAugust 22, 2023Asaf Azulay, Liel Cohen-Lavi, Lilach M. Friedman, Maureen A. McGargill, Tomer Hertzwashed five times with PBS-T washing buffer (0.1% Tween 20 in PBS, 60 μL per well) using a plate washer (ELx405 Select Deep-Well Microplate Washer; BioTek, USA). Plates were then blocked with 100 μL of 10% skim milk powder

(7) The role of B cells in immune cell activation in polycystic ovary syndromeeLifeJuly 4, 2023Angelo Ascani, Sara Torstensson, Sanjiv Risal, Haojiang Lu, Gustaw Eriksson, Congru Li, Sabrina Teschl, Joana Menezes, Katalin Sandor, Claes Ohlsson, et al.solution in water) and a Synergy 2 Luminometer (BioTek, Winooski, VT). Washing steps were performed on an ELx405 Select Deep Well Microplate Washer (BioTek) with PBS or PBS-EDTA. Internal controls were included on each microtiter

(8) Flow-Based CL-SMIA for the Quantification of Protein Biomarkers from Nasal Secretions in Comparison with Sandwich ELISABiosensorsJune 22, 2023Neumair J, Kröger M, Stütz E, Jerin C, Chaker AM, Schmidt-Weber CB, Seidel M.(RT). Afterwards, the ELISA plate was washed three times with a 400 μL washing buffer, each with the ELx405 Select plate washer from BioTek, Winooski, VT, USA. Residual active sites were blocked with 300 μL of a 1%

(9) Inosine Induces Stemness Features in CAR T cells and Enhances PotencybioRxivApril 29, 2023Dorota D. Klysz, Carley Fowler, Meena Malipatlolla, Lucille Stuani, Katherine A. Freitas, Stefanie Meier, Bence Daniel, Katalin Sandor, Peng Xu, Jing Huang, et al.with modifications as described. Briefly, beads were added to a 96-well plate and washed in a BioTek ELx405 Select Deep Well Washer. Samples were added to the plate containing the mixed antibody-linked beads and incubated

(10) Plasmodium falciparum adapts its investment into replication versus transmission according to the host environmenteLifeMarch 14, 2023Abdirahman I Abdi, Fiona Achcar, Lauriane Sollelis, João Luiz Silva-Filho, Kioko Mwikali, Michelle Muthui, Shaban Mwangi, Hannah W Kimingi, Benedict Orindi, Cheryl Andisi Kivisi, et al.following day, the plates were washed thrice with 1 × PBS/0.05% Tween-20 (Sigma-Aldrich) using a BioTek ELx405 Select washer (BioTek Instruments, USA) and blotted on absorbent paper to remove residual buffer. These plates

(11) FLU‐LISA ( fluorescence‐linked immunosorbent assay ) : high‐throughput antibody profiling using antigen microarraysImmunology & Cell BiologyMarch 1, 2023Shlomia Levy, Marwa Abd Alhadi, Asaf Azulay, Amit Kahana, Nir Bujanover, Roi Gazit, Maureen A McGargill, Lilach M Friedman, Tomer Hertzwashed five times with PBS‐T washing buffer (0.1% Tween‐20 in PBS, 60 μL per well) using a plate washer (ELx405 Select Deep‐Well Microplate Washer; BioTek, USA). Plates were then blocked with 100 μL of 10% skim milk powder

(12) Altered meningeal immunity contributing to the autism-like behavior of BTBR T (+) Itpr3 (tf) /J miceBrain, behavior, & immunity - healthDecember 1, 2022Uddin MN, Manley K, Lawrence DA.(3,3′,5,5′-tetramethylbenzidine) was used as substrate (Sigma). Plates were washed with an automated plate washer (BioTek ELx405Select CW, Winooski, VT) and read the absorbance at OD450 with an ELISA plate reader (BioTek EL808). Sera were

(13) A Specific Calprotectin Neo-epitope [CPa9-HNE] in Serum from Inflammatory Bowel Disease Patients Is Associated with Neutrophil Activity and Endoscopic SeverityJournal of Crohn's and ColitisSeptember 8, 2022Joachim Høg Mortensen et al.

(14) Serological Biomarkers of Intestinal Collagen Turnover Identify Early Response to Infliximab Therapy in Patients With Crohn’s DiseaseFrontiers in MedicineJuly 12, 2022Marta S. Alexdottir et al.

(15) Performance of the S taphGold ELISA test in determining subclinical Staphylococcus aureus infections in dairy cows using a Gaussian mixture modelVeterinary Medicine and ScienceJuly 1, 2022Danchen Aaron Yang, Richard Anthony Laven

(16) Sorafenib and nitazoxanide disrupt mitochondrial function and inhibit regrowth capacity in three-dimensional models of hepatocellular and colorectal carcinomaScientific reportsMay 27, 2022Ek F, Blom K, Selvin T, Rudfeldt J, Andersson C, Senkowski W, Brechot C, Nygren P, Larsson R, Jarvius M, et al.solution was obtained. The plates were centrifuged for 5 min at 200g, the Accumax™ was aspirated using Elx405 Select Deep Well Washer (BioTek), and 50 µl new medium was added per well. From each well 10 µl was transferred

(17) High-throughput cell-based assays for identifying antagonists of multiple smoking-associated human nicotinic acetylcholine receptor subtypesSLAS DiscoveryJanuary 21, 2022Michelle Kassner, J. Brek Eaton, Nanyun Tang, Joachim L. Petit, Nathalie Meurice, Hongwei Holly Yin, Paul Whiteakerplates washed one time with wash buffer (1x HBSS with 20 mM HEPES adjusted to pH 7.4) using a BioTek ELx405 Select CW Plate Washer. Twenty-five microliters of 0.5x FLIPR Membrane Potential dye (Molecular Devices, R8034;

(18) Pathologic Proteolytic Processing of N-Cadherin as a Marker of Human Fibrotic DiseaseCellsJanuary 4, 2022Paul Durham Ferrell, Kristianne Michelle Oristian, Everett Cockrell, Salvatore Vincent Pizzowashes (300 µL/well) were performed between each of the following steps using Biotek (Winooski, VT, USA) ELx405 Select CW automated plate washer. All steps were performed at room temperature with room temperature equilibrated

(19) Urodynamic evaluation of patients with localized prostate cancer before and 4 months after robotic radical prostatectomyScientific ReportsDecember 1, 2021Dong Sup Lee, Seung-ju Leewashing buffer (300 μl) using a multichannel pipette (Brand Tech Scientific, MD, USA) or autowasher (ELx405 Select Deep Well Washer, BioTek, VT, USA). Then, 100 μl of biotinylated antibody was applied to each well and

(20) Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and ProductsBiosensors (Basel)July 11, 2021Anna Raysyan, Rudolf J. Schneider

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