Wellwash™ Versa Microplate Washer from Thermo Fisher Scientific

Product Specs
ItemWellwash™ Versa Microplate Washer
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog Number5165010
Manifold ChannelsTwo 1x8, two 1x12, 2x8 cell wash head
Wash Volume50 to 400µL (96 wells) / 20 to 120µL (384 wells)
Residual Volume< 1.5µL
Plate Formats96- and 384-well
Quantity1

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Human urinary extracellular vesicle preparations inhibit in vitro biofilm formation against several uropathogensFront MicrobiolMarch 11, 2026Aziz Ur Rehman, Kieran Abbott, Fiona E. Karet Frankl, Ashraf Zarkan, Tim L. Williamstemperature. A total of 250 μL of 0.25% safranin solution (Sigma-Aldrich) was added to each well using the Wellwash Versa microplate washer (ThermoScientific). The plate was incubated with the stain for 10 min, after which the stain was removed,

(2) Modular DNA barcoding of nanobodies enables multiplexed in situ protein imaging and high-throughput biomolecule detectioneLifeJuly 22, 2025Zhong S, Wang R, Gao X, Guo Q, Lin R, Luo M.the microplate at RT overnight and washed three times with plate wash buffer using microplate washer (Wellwash Versa Microplate Washer, Thermo Fisher Scientific). After incubation in blocking buffer for 1 hr at RT and three times of plate

(3) LB-100 Enhances Drugs Efficacy Through Inhibition of P-Glycoprotein Expression in Multidrug-Resistant Glioblastoma and Non-Small Cell Lung Carcinoma Cellular ModelsPharmaceuticsFebruary 4, 2025Ana Podolski-Renić, Margarita Chigriai, Sofija Jovanović Stojanov, Marija Grozdanić, Ema Lupšić, Igor Nikolić, Miodrag Dragoj, Jelena Dinić, Milica PešićAfter the treatments, the cells were fixed in 4% paraformaldehyde for 20 min at RT and washed using the Wellwash™ Versa microplate washer (Thermo Fisher Scientific, Waltham, MA, USA). The cells were then blocked with 2% bovine serum albumin (BSA

(4) Humoral and cellular immune durability of different COVID-19 vaccine platforms following homologous/heterologous boosters: one-year post vaccinationFrontiers in ImmunologyJanuary 22, 2025Maaweya Awadalla, Halah Z. AlRawi, Rahaf A. Henawi, Fawziya Barnawi, Haitham Alkadi, Ahmed Alyami, Ammar Alsughayir, Alyazeed S. Alsaif, Ayman Mubarak, Wael Alturaiki, et al.ACE2 (His Tag) recombinant protein was used (all provided with the kit. Washing was performed using Wellwash™ Versa Microplate Washer (Thermo Scientific, Waltham, MA USA), and optical density (O.D.) was read at 450 nm using Multiskan FC Microplate Photometer

(5) Multidrug-Resistant Profiles in Non-Small Cell Lung Carcinoma Patient-Derived Cells: Implications for Personalized Approaches with Tyrosine Kinase InhibitorsCancersMay 23, 2024Dinić J, Dragoj M, Jovanović Stojanov S, Stepanović A, Lupšić E, Pajović M, Mohr T, Glumac S, Marić D, Ercegovac M, et al.considered as stromal cells. Cells were fixed in 4% paraformaldehyde for 20 min at RT and washed using the Wellwash™ Versa microplate washer (Thermo Fisher Scientific, Waltham, MA, USA). The cells were then blocked with 2% bovine serum albumin (BSA

(6) KcsA-Kv1.x chimeras with complete ligand-binding sites provide improved predictivity for screening selective Kv1.x blockersThe Journal of biological chemistryApril 1, 2024Szekér P, Bodó T, Klima K, Csóti Á, Hanh NN, Murányi J, Hajdara A, Szántó TG, Panyi G, Megyeri M, et al.Following blocking, the wells were washed 4× with washing buffer (300 μl TBS-Tween, 0.1% v/v) using a Wellwash Versa microplate washer (Thermo Fisher Scientific); then 50 μl phage solution (in TBS-BSA-Tween, 0.1% v/v, 2 mM DDM) at determined

(7) Effects of Weizhuan'an on rats with precancerous lesions of gastric cancer based on regulating gastric mucosal microflora and inflammatory factorsFrontiers in pharmacologyJanuary 1, 2024Lu Y, Liu H, Shang J, Mao Y, Meng L, Gao C.rat inflammation array Q1 (QAR-INF-1) kit (RayBiotech, United States); InnoScan 300 Microarray scanner (Innopsys, France); Wellwash Versa Microplate Washer (Thermo Scientific, United States); RT-6100 Microplate Reader (Rayto, China); F6/10 Micropipette Grinder

(8) Immunofluorescence-Based Assay for High-Throughput Analysis of Multidrug Resistance Markers in Non-Small Cell Lung Carcinoma Patient-Derived CellsDiagnostics (Basel, Switzerland)December 7, 2023Dinić J, Podolski-Renić A, Dragoj M, Jovanović Stojanov S, Stepanović A, Lupšić E, Pajović M, Jovanović M, Petrović Rodić D, Marić D, et al.cells were fixed with 4% paraformaldehyde for 20 min at RT and washed three times with PBS using the Wellwash Versa microplate washer (Thermo Fisher Scientific, Waltham, MA, USA). The cells were then blocked with 2% bovine serum albumin (BSA

(9) Self-Adjuvanting Calcium-Phosphate-Coated Microcrystal-Based Vaccines Induce Pyroptosis in Human and Livestock Immune CellsVaccines (Basel)July 11, 2023Yolanda Corripio-Miyar, Clair Lyle MacLeod, Iris Mair, Richard J. Mellanby, Barry D. Moore, Tom N. McNeillyAll washing steps were carried out 6 × with PBS + 0.05% Tween 20 (PBST), using an automatic washer (Thermo Scientific Wellwash™ Versa, ThermoFisher). Initially, plates were incubated with capture antibody (polyclonal anti-bovine IL-1β,

(10) Follow-Up of PRRSv-Vaccinated Piglets Born from PRRSv-Vaccinated, ELISA-Seropositive and ELISA-Seronegative SowsVirusesFebruary 9, 2023Jorian Fiers, Marylène Tignon, Dominiek Maes, Ann-Brigitte Cayto the manufacturers’ guidelines, without modifications. All washing steps were performed using the WellWash Versa Microplate Washer (Thermo Fisher Scientific, Waltham, MA, USA). Absorbance at 650 nm (ELISA 1), 450 nm (ELISA 2) and 405

(11) Association between the expression of toll-like receptors, cytokines, and homeostatic chemokines in SARS-CoV-2 infection and COVID-19 severityHeliyonJanuary 1, 2023Alturaiki W, Alkadi H, Alamri S, Awadalla ME, Alfaez A, Mubarak A, Alanazi MA, Alenzi FQ, Flanagan BF, Alosaimi B.and ELISA reader were used (Well Wash Versa Microplate Washer and Multiskan FC Microplate Photometer 5165010 & 51119000; Thermo Scientific, Vantaa, Finland). 2.5 Statistical analysis Quantification of circulating

(12) Functionally distinct T-helper cell phenotypes predict resistance to different types of parasites in a wild mammalScientific ReportsDecember 1, 2022Yolanda Corripio-Miyar, Adam Hayward, Hannah Lemon, Amy R. Sweeny, Xavier Bal, Fiona Kenyon, Jill G. Pilkington, Josephine M. Pemberton, Daniel H. Nussey, Tom N. McNeillyperformed 6 times with 350 μl washing buffer (Phosphate Buffered Saline (PBS) + 0.05% Tween 20) using a Thermo Scientific Wellwash™ Versa (ThermoFisher). High-binding capacity ELISA plates (Immunlon™ 2HB 96-well microtiter plates, ThermoFisher)

(13) Using a competitive counterflow assay to identify novel cationic substrates of OATP1B1 and OATP1B3Frontiers in PhysiologySeptember 8, 2022Regina D. Schnegelberger, Brianna Steiert, Philip J. Sandoval, Bruno Hagenbuchwas stopped by washing the wells three times with 100 µl of room temperature uptake solution with a Wellwash™ Versa microplate washer (Thermo Fisher Scientific Inc., Waltham, MA); cells were solubilized with 150 μl of Triton X-100. One

(14) Porcine Reproductive and Respiratory Syndrome virus (PRRSv): A Cross-Sectional Study on ELISA Seronegative, Multivaccinated SowsVirusesAugust 31, 2022Jorian Fiers, Marylène Tignon, Ann Brigitte Cay, Xavier Simons, Dominiek Maesseronegative status. All ELISA analyses were performed according to the manufacturers’ guidelines. The WellWash Versa Microplate Washer (Thermo Fisher Scientific, Waltham, MA, USA) was used for all washing steps, and the MultiSkan FC Microplate Photometer

(15) Serum Calprotectin - a NET Product - as a Biomarker of Disease Activity in Patients with Systemic Lupus Erythematosus: A Single-Center Case-Control Study from PolandMedical science monitor : international medical journal of experimental and clinical researchJuly 13, 2022Homa-Mlak I, Mazurek M, Majdan A, Mlak R, Majdan M, Mełecka-Massalska T.diluted (1: 10) serum samples with dilution buffer and incubated at 37°C for 90 min. After washing twice (Wellwash Versa Microplate Washer, Thermo Scientific), 0.1 ml of diluted biotin-labeled antibody (1: 100) was added and the plate was

(16) Communication Pattern Changes Along With Declined IGF1 of Immune Cells in COVID-19 Patients During Disease ProgressionFrontiers in ImmunologyJanuary 14, 2022Min Zhao, Zhen Liu, Fei Shao, Wenjing Zhou, Zhu Chen, Pengyan Xia, Shuo Wang, Penghui YangUSA). Samples were diluted three times and incubated at room temperature for 1 h. After washing with Wellwash Versa Microplate Washer (Thermo Scientific, Waltham, USA), the slides were incubated with detection antibody for 2 h. Then,

(17) Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)PLOS Neglected Tropical DiseasesDecember 1, 2021Elif Karaaslan, Nesibe Selma Çetin, Merve Kalkan-Yazıcı, Sevde Hasanoğlu, Faruk Karakeçili, Aykut Özdarendeli, Ahmet Kalkan, Ali Osman Kılıç, Mehmet Ziya Doymazin iMark microplate reader (Bio-Rad, California, USA) at 450 nm. All washing steps performed using Wellwash Versa Microplate Washer (Thermo Fisher Scientific, Waltham, USA). Data have shown here represent the mean of three independent

(18) Cellular and humoral immune responses associated with protection in sheep vaccinated against Teladorsagia circumcinctaVeterinary ResearchDecember 1, 2021Cynthia Machín et al.performed 6 times with 350 μL washing buffer (Phosphate Buffered Saline (PBS) + 0.05% Tween®20) using a Thermo Scientific Wellwash™ Versa (ThermoFisher). Briefly, high-binding capacity ELISA plates (Immunolon™ 2HB 96-well microtiter plates,

(19) Cytokine expressions of spinal cord injury treated by neurotropin and nafamostat mesylateAnnals of Translational MedicineApril 14, 2021Chao Sun, Bo Li, Huiquan Duan, Bo Tao, Chenxi Zhao, Wenxiang Li, Yilin Pang, Baoyou Fan, Shiqing Fengwas filled with 100 μL sample (500 μg/mL) and incubated at 4 ℃ overnight. Then, the slides were washed with Thermo Scientific Wellwash Versa (Thermo Scientific, Waltham, MA, USA) and incubated with biotinylated antibody cocktail and Cy3 equivalent

(20) Identification of Differential Expression Cytokines in Hemolysis, Elevated Liver Enzymes, and Low Platelet Syndrome by Proteome Microarray Analysis and Further VerificationCell TransplantationMarch 24, 2021Suya Kang, Liping Zhou, Yun Wang, Hui Li, Hong Zhangmain materials required were InnoScan 300 microarray scanner (Innopsys Company, Carbonne, France) and Thermo Scientific Wellwash Versa (Thermo Fisher Scientific Inc., Waltham, MA, USA). The RayBio array data analysis tool (catalog number:

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells). read more

Thermo Scientific Wellwash Versa

Wellwash™ Versa Microplate Washer from Thermo Fisher Scientific

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Thermo Fisher Scientific

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Bench Tips

Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

Wellwash™ Versa Microplate Washer from Thermo Fisher Scientific

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