Wellwash™ Versa Microplate Washer from Thermo Fisher Scientific

Product Specs
ItemWellwash™ Versa Microplate Washer
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog Number5165010
Quantity1
Plate Formats96- and 384-well
Residual Volume< 1.5µL
Wash Volume50 to 400µL (96 wells) / 20 to 120µL (384 wells)
Manifold ChannelsTwo 1x8, two 1x12, 2x8 cell wash head

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Immunofluorescence-Based Assay for High-Throughput Analysis of Multidrug Resistance Markers in Non-Small Cell Lung Carcinoma Patient-Derived CellsDiagnostics (Basel)December 7, 2023Jelena Dinić, Ana Podolski-Renić, Miodrag Dragoj, Sofija Jovanović Stojanov, Ana Stepanović, Ema Lupšić, Milica Pajović, Mirna Jovanović, Dušica Petrović Rodić, Dragana Marić, et al.cells were fixed with 4% paraformaldehyde for 20 min at RT and washed three times with PBS using the Wellwash Versa microplate washer (Thermo Fisher Scientific, Waltham, MA, USA). The cells were then blocked with 2% bovine serum albumin (BSA

(2) Self-Adjuvanting Calcium-Phosphate-Coated Microcrystal-Based Vaccines Induce Pyroptosis in Human and Livestock Immune CellsVaccines (Basel)July 11, 2023Yolanda Corripio-Miyar, Clair Lyle MacLeod, Iris Mair, Richard J. Mellanby, Barry D. Moore, Tom N. McNeillyAll washing steps were carried out 6 × with PBS + 0.05% Tween 20 (PBST), using an automatic washer (Thermo Scientific Wellwash™ Versa, ThermoFisher). Initially, plates were incubated with capture antibody (polyclonal anti-bovine IL-1β,

(3) Association between the expression of toll-like receptors, cytokines, and homeostatic chemokines in SARS-CoV-2 infection and COVID-19 severityHeliyonJanuary 1, 2023Alturaiki W, Alkadi H, Alamri S, Awadalla ME, Alfaez A, Mubarak A, Alanazi MA, Alenzi FQ, Flanagan BF, Alosaimi B.and ELISA reader were used (Well Wash Versa Microplate Washer and Multiskan FC Microplate Photometer 5165010 & 51119000; Thermo Scientific, Vantaa, Finland). 2.5 Statistical analysis Quantification of circulating

(4) Functionally distinct T-helper cell phenotypes predict resistance to different types of parasites in a wild mammalScientific ReportsDecember 1, 2022Yolanda Corripio-Miyar, Adam Hayward, Hannah Lemon, Amy R. Sweeny, Xavier Bal, Fiona Kenyon, Jill G. Pilkington, Josephine M. Pemberton, Daniel H. Nussey, Tom N. McNeillyperformed 6 times with 350 μl washing buffer (Phosphate Buffered Saline (PBS) + 0.05% Tween 20) using a Thermo Scientific Wellwash™ Versa (ThermoFisher). High-binding capacity ELISA plates (Immunlon™ 2HB 96-well microtiter plates, ThermoFisher)

(5) Cellular and humoral immune responses associated with protection in sheep vaccinated against Teladorsagia circumcinctaVeterinary ResearchDecember 1, 2021Cynthia Machín et al.performed 6 times with 350 μL washing buffer (Phosphate Buffered Saline (PBS) + 0.05% Tween®20) using a Thermo Scientific Wellwash™ Versa (ThermoFisher). Briefly, high-binding capacity ELISA plates (Immunolon™ 2HB 96-well microtiter plates,

(6) Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)PLOS Neglected Tropical DiseasesDecember 1, 2021Elif Karaaslan, Nesibe Selma Çetin, Merve Kalkan-Yazıcı, Sevde Hasanoğlu, Faruk Karakeçili, Aykut Özdarendeli, Ahmet Kalkan, Ali Osman Kılıç, Mehmet Ziya Doymazin iMark microplate reader (Bio-Rad, California, USA) at 450 nm. All washing steps performed using Wellwash Versa Microplate Washer (Thermo Fisher Scientific, Waltham, USA). Data have shown here represent the mean of three independent

(7) Cytokine expressions of spinal cord injury treated by neurotropin and nafamostat mesylateAnnals of Translational MedicineApril 14, 2021Chao Sun, Bo Li, Huiquan Duan, Bo Tao, Chenxi Zhao, Wenxiang Li, Yilin Pang, Baoyou Fan, Shiqing Fengwas filled with 100 μL sample (500 μg/mL) and incubated at 4 ℃ overnight. Then, the slides were washed with Thermo Scientific Wellwash Versa (Thermo Scientific, Waltham, MA, USA) and incubated with biotinylated antibody cocktail and Cy3 equivalent

(8) Identification of Differential Expression Cytokines in Hemolysis, Elevated Liver Enzymes, and Low Platelet Syndrome by Proteome Microarray Analysis and Further VerificationCell TransplantationMarch 24, 2021Suya Kang, Liping Zhou, Yun Wang, Hui Li, Hong Zhangmain materials required were InnoScan 300 microarray scanner (Innopsys Company, Carbonne, France) and Thermo Scientific Wellwash Versa (Thermo Fisher Scientific Inc., Waltham, MA, USA). The RayBio array data analysis tool (catalog number:

(9) Discordance between Serum Neutralizing Antibody Titers and the Recovery from COVID-19The Journal of ImmunologyNovember 15, 2020Merve Kalkan Yazıcı, Meliha Meric Koç, Nesibe Selma Çetin, Elif Karaaslan, Gulay Okay, Bulent Durdu, Bilge Sümbül, Mehmet Z. Doymazwas measured in iMark microplate reader (BioRad) at 415 nm. All washing steps were performed using Wellwash Versa Microplate Washer (Thermo Fisher Scientific). The cut off value between the positive and negative samples was determined

(10) Survival of recombinant monoclonal and naturally-occurring human milk immunoglobulins A and G specific to respiratory syncytial virus F protein across simulated human infant gastrointestinal digestionJournal of Functional FoodsOctober 26, 2020Jiraporn Lueangsakulthai, Baidya Nath P. Sah, Brian P. Scottoline, David C. Dallastimes with 200 μL of 0.05% Tween-20 (Bio-Rad, Rockford, IL, USA) in phosphate-buffered saline using a Wellwash™ Versa microplate washer (Thermo Scientific). To prevent the nonspecific binding of the antibodies, plates were blocked with

(11) Analysis of vascular‑associated factors and the prognosis of poorly differentiated hypopharyngeal carcinomaOncology LettersSeptember 21, 2020Wei Guo, Gaofei Yin, Hongfei Liu, Hanyuan Duan, Zhigang Huangovernight at 4°C (the sample was centrifuged at room temperature at 7.2 × g for 4 min; 0.5 mg/ml). A Wellwash Versa Microplate Washer (Thermo Fisher Scientific, Inc.) was used to clean the slides. A total of 70 µl biotin was added to

(12) Co-transplantation of bone marrow mesenchymal stem cells and monocytes in the brain stem to repair the facial nerve axotomyEuropean Journal of HistochemistryJune 19, 2020Li Wu, Dan Han, Jie Jiang, Xiaojie Xie, Xunran Zhao, Tengfei Ke, Wen Zhao, Liu Liu, Wei Zhaomg/mL) was added into each well and the chip was incubated at 4°C overnight. After washing with the Thermo Scientific Wellwash Versa chip washing machine (Thermo Scientific, Waltham, MA, USA), 70 mL biotin-labeled antibody was added

(13) Quantitative Analysis of Antibody Survival across the Infant Digestive Tract Using Mass Spectrometry with Parallel Reaction MonitoringFoodsJune 9, 2020Bum Jin Kim, Jiraporn Lueangsakulthai, Baidya Nath P. Sah, Brian Scottoline, David C. Dallasat 4 °C. After incubation, the microtiter plate was washed three times with 200 μL of PBST using a Wellwash™ Versa microplate washer (Thermo Scientific, Waltham, MA, USA) and blocked for 1 h with 150 μL of 5% of bovine serum albumin

(14) Survival of Recombinant Monoclonal Antibodies (IgG, IgA and sIgA) Versus Naturally-Occurring Antibodies (IgG and sIgA/IgA) in an Ex Vivo Infant Digestion ModelNutrientsFebruary 27, 2020Jiraporn Lueangsakulthai, Baidya Nath P. Sah, Brian P. Scottoline, David C. Dallasplates were washed three times with 200 μL of phosphate-buffered saline with 0.05% Tween-20 using a Wellwash™ Versa microplate washer (Thermo Scientific). To prevent nonspecific binding of the antibodies, plates were blocked with 150

(15) ELISA Based Protein Ubiquitylation MeasurementBIO-PROTOCOLNovember 20, 2019Yuka Kamada, Ryosuke Fukuda, Tsukasa Okiyoneda5250040) 2. WellwashTM Versa Microplate Washer (Thermo Fisher Scientific, Thermo Scientific, catalog number: 5165010) 3. 8 channel electronic pipette (Sartorius, catalog number: 730390) and 50-1,200 μl tips (Sartorius, catalog

(16) Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapyPloS oneJanuary 1, 2019Giampieri R, Piva F, Occhipinti G, Bittoni A, Righetti A, Pagliaretta S, Murrone A, Bianchi F, Amantini C, Giulietti M, et al.recorded at 450 nm by Multiskan FC Microplate Photometer (Thermo Fisher Scientific). Washings were done by Wellwash Versa Microplate Washer (Thermo Fisher Scientific) to assure intra-assay repeatability and incubations were done in dark conditions.

(17) 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattleBMC Veterinary ResearchDecember 13, 2017Yolanda Corripio-Miyar, Richard J. Mellanby, Katy Morrison, Tom N. McNeillyand washing steps were performed 6 times with 350 μl washing buffer (PBS + 0.05% Tween 20) using a Thermo Scientific Wellwash™ Versa (ThermoFisher). Briefly, high-binding capacity ELISA plates (Immunolon™ 2HB 96-well microtiter plates,

(18) Seroprevalence and Knowledge of Hepatitis B Virus Infection Among LaboratoryWorkers at Kilimanjaro Christian Medical Centre in Moshi, TanzaniaEast Afr Health Res JJuly 1, 2017Richard E Machange, Dominic Mosha, Jeremia J Pyuza, Balthazar B Nyombi, Elichilia R Shaoadded to antigens or antibodies fixed to a solid surface microplate, incubated, and then washed using Thermo Scientific Wellwash Versa. The samples were then taken to Thermo Scientific Multiskan FC microplate photometer reader, with Skanlt

(19) Seroprevalence and Knowledge of Hepatitis B Virus Infection Among Laboratory Workers at Kilimanjaro Christian Medical Centre in Moshi, TanzaniaEast African Health Research JournalMay 17, 2017Richard E Machange, Dominic Mosha, Jeremia J Pyuza, Balthazar B Nyombi, Elichilia R Shaoadded to antigens or antibodies fixed to a solid surface microplate, incubated, and then washed using Thermo Scientific Wellwash Versa. The samples were then taken to Thermo Scientific Multiskan FC microplate photometer reader, with Skanlt

(20) The dehydrogenase region of the NADPH oxidase component Nox2 acts as a protein disulfide isomerase (PDI) resembling PDIA3 with a role in the binding of the activator protein p67 (phox.)Frontiers in chemistryJanuary 1, 2015Bechor E, Dahan I, Fradin T, Berdichevsky Y, Zahavi A, Federman Gross A, Rafalowski M, Pick E.was removed by repeated washing with PBS supplemented with 0.1% v/v Tween 20 (Sigma-Aldrich), using a Wellwash Versa microplate washer (Thermo Fisher Scientific). Next, the surface-attached p67phox was exposed to 200 μl/well of NusA-Nox2

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells). read more

Thermo Scientific Wellwash Versa

Wellwash™ Versa Microplate Washer from Thermo Fisher Scientific

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Thermo Fisher Scientific

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Bench Tips

Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

Wellwash™ Versa Microplate Washer from Thermo Fisher Scientific

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