Agilent BioTek Gen5 Software for Imaging & Microscopy from Agilent Technologies

Product Specs
ItemAgilent BioTek Gen5 Software for Imaging & Microscopy
CompanyAgilent Technologies
Price Get Pricing Get Quote for this Product
Catalog NumberGen5
QuantityEA
ApplicationsMicroplate Reading and Imaging

Specifications/Features

Automated Image Capture, Z-stacking, Montaging, Kinetic analysis, Image Processing, Image Analysis, Neurite Outgrowth, Spot Counting, Automatic Region-of-Interest, Object Tracking, Scratch Assays

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Agilent Technologies

5301 Stevens Creek Blvd

Santa Clara, California 95051

United States

Phone: +1 877-424-4536

Company Profile

Website: www.agilent.com

(1) Investigating the effects of membrane curvature inducing proteins on lipid droplets in Saccharomyces cerevisiaeMolecular Biology ReportsDecember 1, 2026Laura R. K. Niemelä, Isabell Tunn, Alexander D. Freyrate was calculated using Microsoft Excel and the maximum growth rate was calculated by the BioTek Gen5 (2.9 version) software. Results We investigated the effects of membrane curvature inducing proteins

(2) Aflatoxin B1 contamination and fungal diversity in oilseed cakes and formulated feeds from livestock feed markets in Addis Ababa and surrounding areasMycotoxin ResearchMay 1, 2026Adey Melesse Yalew, Alemayehu Teressa Negawo, Tesfaye Alemu, Jean Hanson, Michael Blummel, Nebiyat Nigusie Woldeyohannis, Yilikal Assefa Teferi, Yonas Asmare, Prasad Kodukula V.S.V., Chris S. Joneson a log scale. The corresponding %B/Bo values were plotted along the y-axis. Reader Fit software (Gen5 a Software Version 2.01.14) was used to fit the standard curve (R2 > 0.99). A Data Analysis Worksheet

(3) Fragment-Derived Nicotinic Acid Analogues Inhibit hCA III and Downregulate CA3 Expression in HepG2 CellsBiomoleculesApril 17, 2026Areej Abuhammad, Tamara Sabri, Nidaa A. Ababneh, Rya A. Ali, Mohammad A. Ismail, Adan Madadha, Dareen T. Yazjeen, Rama J. Alghanem, Ali M. Qaisi, Yusuf Al-Hiari, et al.multimode plate reader (Agilent BioTek, Santa Clara, CA, USA), and data analysis was conducted using Gen5 (Version 3.18.17) software (Agilent BioTek, Santa Clara, CA, USA). 2.9. Assessment of Intracellular

(4) Modifying Effect of the Antibiotic Action, Membrane Permeability, and ADMET/Toxicity Profile of the Fixed Oil From the Pulp of Mauritia Flexuosa F.L. (Arecaceae)Chem BiodiversApril 15, 2026Isaac Moura Araújo, Cleidiana Tomé de Oliveira, Luís Pereira-de-Morais, Andressa Silva Alencar, Cicera Datiane de Morais Oliveira-Tintino, Maria Alícia Cavalcante Narciso, Anita Oliveira Brito Pereira Bezerra Martins, Sara Tavares de Sousa Machado, Sheila Alves Gonçalves, Daniel Sampaio Alves, et al.fluorescence readings were performed using a Cytation 1 plate reader, BioTek (Winooski, VT, USA) and Gen5 3.11 Software. A 485 nm excitation and 528 nm emission filter were used [16]. The tests were performed

(5) Asphalt Binder Modification with Hazelnut and Walnut Shells as Valued Antioxidant Sources: Effects on Rheological and Main Physicochemical Post-Oxidation IndicatorsMaterials (Basel)April 14, 2026Carlos Manterola-Barroso, Karina Godoy-Sánchez, Erick Scheuermann, Ivanka Netinger Grubeša, Dunja Šamec, Cristian Meriño-Gergichevichmeasured with a Biotek Synergy H1 Hybrid multi-mode reader (Winooski, VT, USA) and data was interpreted by Gen5™ V 2.0 software. In order to complement the obtained ORAC data, total phenolic content (TPC) analyses

(6) Establishment and characterization of a new immortalized human adenomyosis epithelial-like cell line, tAEC21†Biology of ReproductionApril 13, 2026Yuliya Klymenko, Jessica L Kersey, Hunter D Quigley, Shannon M HawkinsAbsorbance readings were taken at 490 and 630 nm using a Synergy H1 Hybrid Reader (BioTek, Winooski, VT) and Gen5 3.11 software. Background absorbance was subtracted, and data were normalized to day 1 post-seeding.

(7) Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentationBioact MaterApril 11, 2026Shiyuan Li, Mingyu You, Hao Zheng, Yinchuan Wang, Bozun Miao, Jia Pei, Meiping Xiong, Guangyin Yuan, Liang Songof incubation at 37 °C, the optical density (OD) was measured at 450 nm using a microplate reader (Gen5, BioTek, USA). For live/dead cell staining assays, cells were co-cultured with extracts from various

(8) Cyclopalladated Complexes With Functionalized Diphosphanes as Promising Antifungal ScaffoldsBioinorg Chem ApplApril 11, 2026Cláudia Malta-Luís, Carolina Mariano, Teresa Monteiro, Francisco C. Mendes, María Villar-López, Álvaro J. Arana, Laura Sánchez, Digna Vázquez García, Alberto Fernández, José M. Vila, et al.recorded after 24 h at 35°C by measuring OD530nm using the Epoch Microplate Spectrophotometer and BioTek Gen5 Data Analysis Software. Growth without complexity and with 1% DMSO (control condition) was used for

(9) Chlorotoxin does not target matrix metalloproteinase-2 in glioblastomaPLoS OneApril 9, 2026Eli Blaney, Meron Demeke, Seraphine Kamayirese, Louise Monga, Laura A. Hansen, Charles R. Watts, Sándor LovasFluorescence was measured at excitation/emission wavelengths of 545/576 nm, with a cutoff at 570 nm using Gen5 microplate reader (BioTeK, Shoreline, WA). Baseline fluorescence was recorded before adding NNGH inhibitor

(10) Novel Palmitoyl Pentapeptides: Self-Assembly, Collagen Stimulation, Antioxidant Activity, and Influence of Skin Microbiota HealthACS OmegaApril 7, 2026Juliane N. B. D. Pelin, Lucas R. de de Mello, Raquel Allen Garcia Barbeto Siqueira, Matheus de de Souza Alves, Valeria Castelletto, João Francisco Almeida, Jani Seitsonen, Patricia Santos Lopes, Vânia Leite-Silva, Newton Andreo-Filho, et al.period, optical densities were read using a microplate reader (BioTek, model: Synergy HT, software: Gen5, Agilent, Santa Clara, CA, USA) at a wavelength of 600 nm. Antioxidant Activity Assay in Monolayer HaCaT cells

(11) Imaging–guided optimization of biodistribution and antitumor efficacy of L19-based immunocytokinesTranslational OncologyApril 1, 2026Tommaso Virgilio, Kamil Chahine, Jordi Guixeras Carreras, Alain Pulfer, Chiara Pizzichetti, Irene Latino, Daniel Molina-Romero, Arianna Capucetti, Louis Luca Renner, Dario Neri, et al.221,465), and the 405 nm absorbance was measured using an ELISA micro-plate reader (BioTek) with the Gen5 3.12 software. For in vivo studies, L19-AF647, KSF-AF647, and L19IL2 were injected subcutaneously (1

(12) Doxorubicin enhances adipogenesis in an FGF2-dependent manner and induces a tumour-promoting secretory phenotypeJournal of Bone OncologyApril 1, 2026Lauren M. Kreps, Edward Yakubovich, Huijun Zhao, Selam Yimer, Edward Yang, Juliet Cruz, Barbara Vanderhyden, Christina L. Addisoninstructions. Absorbance was measured using the Biotek Cytation5 Cell Imaging Multimode Reader with the Gen5 Image Prime 3.10 software (Agilent Technologies Canada Inc., Mississauga, ON). FGF2 concentration was

(13) Low‐dose lithium supplementation promotes musculoskeletal and metabolic health in ovariectomized female miceThe Journal of PhysiologyApril 1, 2026Bianca M. Marcella, Jessica L. Braun, Amélie A.T. Marais, Briana L. Hockey, Mia S. Geromella, Ryan W. Baranowski, Rene Vandenboom, Rebecca E.K. MacPherson, Val A. Fajardosettings for three filters: DAPI, GFP and Texas Red. Images were stitched, processed and saved using the Gen5 image processing functions. Analysis was performed using ImageJ (NIH) software and the cell‐counter

(14) Protocol for automated quantification of neuronal cells using the Agilent BioTek Cytation system and Gen5 neurite outgrowth moduleSTAR ProtocApril 1, 2026Aline Broeglin, Olivier Buetzberger, Rebecca Mongeon, Aurélien Riou, Anne Eckert, Amandine GrimmProtocol for automated quantification of neuronal cells using the Agilent BioTek Cytation system and Gen5 neurite outgrowth module

(15) GM-CSF and M-CSF Driven Differentiation Differentially Regulates Chikungunya Virus Infection and Antiviral Responses in Human Monocyte-Derived MacrophagesbioRxivMarch 29, 2026Jeury Veloz, Victoria Zyulina, Sara Thannickal, Eva Chebishev, Dabeiba Bernal-Rubio, Maria del Mar Villanueva Guzman, Chenyang Wu, Estefania Valencia, Danielle Novillo, Vrushali Dhamapurkar, et al.using the imager manual mode at 10x magnification. Initial image deconvolution was performed within the Gen5 Image+ software and final review and processing was performed on ImageJ. Quantification was performed

(16) Antioxidant Potential, Genotoxic Safety, and Metabolomic Profiling of Cladode Extracts from Dactylopius opuntiae-Resistant Opuntia SpeciesAntioxidants (Basel)March 26, 2026Abderrahmane Hadini, Mounsef Neffa, Sanae Baddaoui, Mourad Bendada, Mohammadine Moumou, Amani Tayebi, Kaoutar Aboukhalid, Abdesselam Maatougui, Ennouamane Saalaoui, Maria D’Elia, et al.distilled water was added, and the absorbance was measured at 540 nm using a spectrophotometer Biotek Gen5 microplate reader (BioTek Gen5, Winooski, VT, USA). The calculation of reducing sugars was carried out

(17) Importin α1 contributes to Venezuelan equine encephalitis virus-induced cell death, but is not required for the capsid-mediated blockage of nucleocytoplasmic traffickingFrontiers in MicrobiologyMarch 25, 2026Abdullahi T. Jamiu, Ivan Akhrymuk, Grace Boyer, Gregory M. Petruncio, Maryna Akhrymuk, Bryan M. Delfing, Hui-Chen Foreman, Kenneth W. Foreman, Dmitri K. Klimov, Mikell Paige, et al.protein, either capsid or dTomato, was determined using the cellular analysis tool on Agilent BioTek Gen5 Image Prime 3.14 software (Galenkamp et al., 2021). Fn and Fc are the integral fluorescence signals

(18) Female-enriched Eggerthella lenta drives neuroinflammation and IFN-γ via host receptor TLR2bioRxivMarch 25, 2026Rachel R. Rock, Margaret Alexander, Cecilia Noecker, Kai R. Trepka, Vaibhav Upadhyay, Edwin F. Ortega, Lorenzo Ramirez, Lena Siewert, Christine A. Olson, Taylor Halsey, et al.(v0.6.2),CRAN,RRID:SCR_021243 STAR (v2.7.11),"Dobin et al., 2013",RRID:SCR_004463 FlowJo (v10.10.0),BD,RRID:SCR_008520 Gen5 (v3.08),BioTek,RRID:SCR_017317 CFX384 Real-Time system software (v3.1),BIO-RAD,RRID:SCR_018057 Affinity,Canva,https://www.affinity.studio/download MapChart,MapChart,https://www.mapchart.net/ BioRender,BioRender,RRID:SCR_018361 Other,Other,Other Autoclavable

(19) SkelPy: A graphic user interface–based approach for skeletonizing fungal networksApplications in Plant SciencesMarch 24, 2026Melanie Madrigal, Aaron J. Moseley, Jenna C. Moseley, Jordan A. Dowellwere grown in liquid culture in a 96‐well microplate for 72 h and imaged every 4 h using a Cytation Gen5 plate reader (Agilent Technologies, Santa Clara, California, USA), generating a dataset of 180 time‐series

(20) β-Catenin Stabilization Protects Against Alveolar Hemorrhage Through Amphiregulin and BATF-Mediated Regulatory T CellsJCI InsightMarch 23, 2026Fiona Mason, Hui Xiong, Ali Mobeen, Md Saddam Hossain, Sara Mahmudlu, Rosanne Trevail, Mikyal Mobeen, Li Chen, Sunny Lee, Tuncay Delibasi, et al.at 562 nm using a BioTek FL ×800 plate reader, and protein concentrations (μg/mL) were calculated in Gen5 using a BSA standard curve. Cytokine production assays. Serum was collected from cardiac blood at

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