Multiskan™ FC Microplate Photometer from Thermo Fisher Scientific

Product Specs
ItemMultiskan™ FC Microplate Photometer
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog Number51119100
RangeOptional Up to 50 °C
MethodBy filters
Wavelength(s)340 - 850 nm
Measurement Time7 seconds (96 Wells Fast Mode)
13 seconds (96 Wells Normal Mode)
13 seconds (384 Wells Fast Mode)
34 seconds (384 Wells Normal Mode)
Optical SystemFilters
Plate Formats96- and 384-well plates
Light SourceQuartz-halogen lamp
DetectorPhotodetector
Resolution0.001 Abs
Accuracy±1% (0 to 3 Abs) or ±0.003 Abs, whichever is greater
PrecisionCV ≤0.2% (0.3 to 3 Abs)
QuantityEA
DetectionAbsorbance
ApplicationsELISA, Immunoassays, Protein quantification, Endotoxin, Cytotoxicity and proliferation assays, Enzyme assays and Growth curves

Specifications/Features

Shaking, Temperature Control

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Pirfenidone-Loaded Polymeric Micelles as an Effective Mechanotherapeutic to Potentiate Immunotherapy in Mouse Tumor ModelsACS NanoDecember 26, 2023Fotios Mpekris, Petri Ch. Papaphilippou, Myrofora Panagi, Chrysovalantis Voutouri, Christina Michael, Antonia Charalambous, Mariyan Marinov Dinev, Anna Katsioloudi, Marianna Prokopi-Demetriades, Andreas Anayiotos, et al.dissolved in 100 μL of DMSO (Sigma-Aldrich, USA), and the absorbance was measured at 570 and 690 nm using a Multiskan FC microplate photometer (Thermo Fisher Scientific, USA). The background absorbance at 690 nm was subtracted. Cell viability

(2) Fruits are vehicles of drug-resistant pathogenic Candida tropicalisMicrobiology SpectrumDecember 12, 2023Yin-Zhi Chen, Kuo-Yun Tseng, Si-Chong Wang, Ciao-Lin Huang, Chih-Chao Lin, Zi-Li Zhou, De-Jiun Tsai, Chiao-Mei Lin, Yu-Lian Chen, Kai-Ting Chen, et al.Laboratory Standards Institute (CLSI) document M27-A3 (44). The growth of each isolate was measured by Multiskan FC Microplate Photometer (Thermo Fisher Scientific, USA) after incubation at 35°C for 24 h. Standard powders of two clinical

(3) Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological PotentialPharmaceuticsDecember 7, 2023Natalija Čutović, Tatjana Marković, Tamara Carević, Dejan Stojković, Branko Bugarski, Aleksandra A. Jovanovićwater, air-dried, and then 96% ethanol (Zorka, Šabac, Serbia) was used. Thermo Fisher Scientific’s Multiskan FC Microplate Photometer (Waltham, MA, USA) was used to detect absorbance at 620 nm, and Equation (3) was used to calculate the

(4) Zuccagnia punctata Cav., a Potential Environmentally Friendly and Sustainable Bionematicide for the Control of Argentinean Horticultural CropsPlants (Basel)December 7, 2023Sofía Manrique, Jessica Gómez, Mauricio Piñeiro, Belén Ariza Sampietro, Maria L. Peschiutta, Alejandro Tapia, Mario J. Simirgiotis, Beatriz Limaof quercetin equivalents (QE) per gram of extracts (mg QE/gZeDe). The values were obtained using a Multiskan FC Microplate Photometer (Thermo Scientific, Waltham, MA, USA), and are shown as the mean ± standard deviation (SD) [29]. 2.8.

(5) Protocol for measuring erythrocyte glutathione reductase activity coefficient to assess riboflavin statusSTAR ProtocolsDecember 1, 2023Damon A. Parkington, Albert Koulman, Kerry S. Jonesresources table Materials and equipment CRITICAL: This protocol uses a Thermo Fisher Scientific Multiskan FC microplate photometer (with incubator) to measure a decrease in absorbance at 340 nm at a controlled temperature of 28°C.

(6) Synaptic Involvement of the Human Amygdala in Parkinson’s DiseaseMolecular & Cellular ProteomicsDecember 1, 2023Sandra Villar-Conde, Veronica Astillero-Lopez, Melania Gonzalez-Rodriguez, Daniel Saiz-Sanchez, Alino Martinez-Marcos, Isabel Ubeda-Banon, Alicia Flores-Cuadradoconcentration was measured with the bicinchoninic acid protein determination kit (Sigma‒Aldrich) and a Multiskan FC microplate photometer (Thermo Fisher Scientific). Twenty micrograms of protein per sample was loaded onto 10% polyacrylamide

(7) Optimizing the microbial synthesis of silver nanoparticles using Gloeophyllum striatum and their antimicrobial potential evaluationScientific ReportsNovember 30, 2023Aleksandra Tończyk, Katarzyna Niedziałkowska, Katarzyna Lisowska37 °C. After the required incubation period, the OD was measured at a wavelength of 630 nm using a Multiskan™ FC Microplate Photometer (Thermo Fisher Scientific, Pudong, Shanghai, China), and the MIC was established for each tested variant

(8) Epidermal growth factor potentiates EGFR(Y992/1173)-mediated therapeutic response of triple negative breast cancer cells to cold atmospheric plasma-activated mediumRedox BiologyNovember 29, 2023Peiyu Wang, Renwu Zhou, Rusen Zhou, Shuo Feng, Liqian Zhao, Wenshao Li, Jinyong Lin, Aleksandra Rajapakse, Chia-Hwa Lee, Frank B. Furnari, et al.Stop Solution, successively, with 4 × 5 min washing between each step. The data was immediately collected by Multiskan™ FC Microplate Photometer (Thermo Fisher Scientific, USA) at 450 nm. Based on the EGFR standard curve, the EGFR level of each

(9) Pulsed Electromagnetic Fields Induce Skeletal Muscle Cell Repair by Sustaining the Expression of Proteins Involved in the Response to Cellular Damage and Oxidative StressInternational Journal of Molecular SciencesNovember 23, 2023Silvia Maiullari, Antonella Cicirelli, Angela Picerno, Francesca Giannuzzi, Loreto Gesualdo, Angela Notarnicola, Fabio Sallustio, Biagio Moretti30 min, the absorbance was measured at a wavelength of 450 nm using an ELISA reader for microplates (Multiskan FC Microplate Photometer, cat. no 51119000; Thermo Scientific Inc., Waltham, MA, USA). 4.5. Scratch Assays First, 2 × 105 SkMC/well

(10) Sulfated Triterpene Glycosides from the Far Eastern Sea Cucumber Cucumaria djakonovi: Djakonoviosides C1, D1, E1, and F1; Cytotoxicity against Human Breast Cancer Cell Lines; Quantitative Structure–Activity RelationshipsMarine DrugsNovember 22, 2023Alexandra S. Silchenko, Anatoly I. Kalinovsky, Sergey A. Avilov, Roman S. Popov, Ekaterina A. Chingizova, Ekaterina S. Menchinskaya, Elena A. Zelepuga, Elena G. Panina, Vadim G. Stepanov, Vladimir I. Kalinin, et al.addition of 100 µL of SDS-HCl solution (1 g SDS/10 mL d-H2O/17 µL 6 N HCl) and further incubated for 18 h. Multiskan FC microplate photometer (Thermo Fisher Scientific, Waltham, MA, USA) was used to measure the absorbance of the converted dye

(11) Solanum lycopersicum heme-binding protein 2 as a potent antimicrobial weapon against plant pathogensScientific ReportsNovember 20, 2023Atefeh Farvardin, Eugenio Llorens, Luisa Liu-Xu, Lorena Sánchez-Giménez, Aloysius Wong, Elena G. Biosca, José M. Pedra, Eva Falomir, Gemma Camañes, Loredana Scalschi, et al.to mimic the pH of the apoplast, before adding the bacteria. The growth assay was carried out in a Multiskan FC Microplate Photometer (Thermo Scientific) in a total volume of 200 μL in microtiter wells using a final bacterial concentration

(12) Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acidMicrobial Cell FactoriesNovember 17, 2023Sílvia Àvila-Cabré, Míriam Pérez-Trujillo, Joan Albiol, Pau Ferrerconcentration of each deep-well was determined in duplicate with a 96-well microtiter plate using a Multiskan FC Microplate Photometer (Thermo Fisher Scientific, Waltham, MA, USA) to ensure all the cultures were grown up to a similar endpoint

(13) Dynamic social interactions and keystone species shape the diversity and stability of mixed-species biofilms – an example from dairy isolatesISME CommunicationsNovember 15, 2023Faizan Ahmed Sadiq, Koen De Reu, Hans Steenackers, Ann Van de Walle, Mette Burmølle, Marc Heyndrickx°C followed by appropriate dilution in fresh BHI to the OD595 value of 0.05 for all strains using a Multiskan ™ FC Microplate Photometer (Thermo Fisher Scientific). A total of 160 μL was used as the inoculum volume for single-species biofilms,

(14) In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract InfectionsPharmaceuticals (Basel)November 14, 2023Dragana D. Božić, Ivana Ćirković, Jovica Milovanović, Biljana Bufan, Miljan Folić, Katarina Savić Vujović, Bojan Pavlović, Ana Jotić(Sigma-Aldrich-Merck, Cat. No. 1590102500) and the presence of biofilm was measured at an optical density of 570 nm (Multiskan™ FC Microplate Photometer, ThermoFisher Scientific, Waltham, MA, USA). The category of biofilm production was calculated in relation

(15) 4-(3-Phenyl-4-(3,4,5-trimethoxybenzoyl)-1H-pyrrol-1-yl)benzenesulfonamide, a Novel Carbonic Anhydrase and Wnt/β-Catenin Signaling Pathway Dual-Targeting Inhibitor with Potent Activity against Multidrug Resistant Cancer CellsJournal of Medicinal ChemistryNovember 9, 2023Domiziana Masci, Michela Puxeddu, Laura Di Magno, Michele D’Ambrosio, Anastasia Parisi, Marianna Nalli, Ruoli Bai, Antonio Coluccia, Pietro Sciò, Viviana Orlando, et al.nm along with the reference wavelength at 650 nm was measured using a microplate spectrophotometer (Multiskan FC Microplate Photometer, Thermo Scientific, Waltham, MA, USA). The cell growth inhibition rate was calculated utilizing the

(16) Making watercress (Nasturtium officinale) cropping sustainable: genomic insights into enhanced phosphorus use efficiency in an aquatic cropFrontiers in Plant ScienceNovember 7, 2023Lauren E. Hibbert, Yufei Qian, Hazel K. Smith, Suzanne Milner, Ella Katz, Daniel J. Kliebenstein, Gail Taylor0.1% w/v) was added per well and incubated for 20 min at 100°C. Absorbance at 620 nm was analyzed on a Multiskan™ FC Microplate Photometer (Thermo Scientific). Lyophilized root and shoot tissue was also used for quantification of P and potassium

(17) Antifungal Activity and Type of Interaction of Melissa officinalis Essential Oil with Antimycotics against Biofilms of Multidrug-Resistant Candida Isolates from Vulvovaginal MucosaJournal of FungiNovember 4, 2023Marina Ranđelović, Marina Dimitrijević, Suzana Otašević, Ljiljana Stanojević, Milica Išljamović, Aleksandra Ignjatović, Valentina Arsić-Arsenijević, Zorica Stojanović-RadićMonitoring of biofilm growth was conducted based on absorbance measurements at 595 nm using an ELISA reader (Multiskan™ FC Microplate Photometer, Thermo Scientific™, Waltham, MA, USA). The strains were classified into the following four categories

(18) Anti-inflammatory, Anti-fibrotic and Pro-cardiomyogenic Effects of Genetically Engineered Extracellular Vesicles Enriched in miR-1 and miR-199a on Human Cardiac FibroblastsStem Cell Reviews and ReportsNovember 1, 2023Katarzyna Kmiotek-Wasylewska, Sylwia Bobis-Wozowicz, Elżbieta Karnas, Monika Orpel, Olga Woźnicka, Zbigniew Madeja, Buddhadeb Dawn, Ewa K. Zuba-Surmacells cultured in standard conditions (21% O2) or in hypoxia (1% O2). Absorbance was measured using Multiskan FC Microplate Photometer (Thermo Fisher). Metabolic Activity Analysis ATP production was measured with the ATPLite Kit (PerkinElmer

(19) Benzimidazoles Containing Piperazine Skeleton at C-2 Position as Promising Tubulin Modulators with Anthelmintic and Antineoplastic ActivityPharmaceuticals (Basel)October 25, 2023Kameliya Anichina, Anelia Mavrova, Dimitar Vuchev, Galya Popova-Daskalova, Giada Bassi, Arianna Rossi, Monica Montesi, Silvia Panseri, Filip Fratev, Emilia Naydenovaof incubation with gentle stirring, the absorbance of formazan was read at 570 nm measured using a Multiskan FC Microplate Photometer from Thermo Fisher Scientific. The absorbance values were directly proportional to the number of metabolically

(20) Does Nirmatrelvir/Ritonavir Influence the Immune Response against SARS-CoV-2, Independently from Rebound?MicroorganismsOctober 22, 2023Francesca Panza, Fabio Fiorino, Gabiria Pastore, Lia Fiaschi, Mario Tumbarello, Donata Medaglini, Annalisa Ciabattini, Francesca Montagnani, Massimiliano FabbianiThermo Fisher Scientific, Waltham, MA, USA) substrate. The absorbance at 450 nm was measured using a Multiskan FC Microplate Photometer (Thermo Fisher Scientific, Waltham, MA, USA). WHO international positive (NIBSC 20/150) and negative

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7/15/2021Multiskan FC Microplate Photometer Works Great Joaquin ValcarcelFor myself and our laboratory team, the Multiskan FC microplate photometer is a fantastic piece of equipment that provides optimal performance and consistent results. Because of the wide wavelength ... Read Review

Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells). read more

Thermo Scientific™ Multiskan™ FC Filter-based Microplate Photometer

Multiskan™ FC Microplate Photometer from Thermo Fisher Scientific

Description

Specifications/Features

Thermo Fisher Scientific

Citations (573)

Reviews

Bench Tips

Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

Multiskan™ FC Microplate Photometer from Thermo Fisher Scientific

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