Mini ELISA Plate Reader™ from BioLegend

Product Specs
ItemMini ELISA Plate Reader™
CompanyBioLegend
Price Get Pricing Get Quote for this Product
Catalog Number423555
Dimensions55 x 96 x 154 mm
ApplicationsELISA, Colorimetric detection
Wavelength(s)405 nm, 450 nm, 492 nm, and 620 nm
Light Source4 LEDs
Detection96 Photodiodes
Accuracy0.0-2.0 OD = (1 % + 0.01 OD), 2.0-3.0 OD = (1.5 % + 0.01 OD)
TypeELISA Plate Reader
Measurement Mode(s)Absorbance
QuantityEA

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Enhancement of CD117-Targeted Bispecific T-cell Engagement by CD33-Targeted Bispecific T-cell Costimulation in Acute Myeloid LeukemiaCancer Res CommunApril 1, 2026Mara Hofstetter, Laura Volta, Christian Koch, Melanie Granados Rey, Monique Maurer, Nagihan Gönüllü, Christian Pellegrino, Celeste Gobbi, Florin Schneiter, Francesco Manfredi, et al.concentrations were measured at an absorbance of 450 nm using a Mini ELISA Plate Reader (BioLegend, cat. #423555). Live-cell imaging Time-lapse experiments were conducted at 37°C and 5% CO2 in RPMI 1640 growth medium

(2) Enhancement of CD117-targeted bispecific T-cell engagement by CD33-targeted bispecific T-cell co-stimulation in acute myeloid leukemiaCancer Research CommunicationsApril 1, 2026Mara Hofstetter, Laura Volta, Christian Koch, Melanie Granados. Rey, Monique Maurer, Nagihan Gönüllü, Christian Pellegrino, Celeste Gobbi, Florin Schneiter, Francesco Manfredi, et al.concentrations were measured at an absorbance of 450 nm using a Mini ELISA Plate Reader (BioLegend, cat. #423555). Live-cell imaging Time-lapse experiments were conducted at 37°C and 5% CO2 in RPMI 1640 growth medium

(3) Immunogenicity of a recombinant subunit COVID-19 vaccine: A prospective, multi-centre, longitudinal study in Chinese Alzheimer's disease patientsHum Vaccin ImmunotherDecember 3, 2025Youhong Weng, Yihua Huang, Jiangtao Zhang, Yahan Wu, Qing He, Sofia Litchev, Aimee Wu, Zongxin Ling, Longyou Zhao, Rongxian Liao, et al.terminate the reaction, and absorbance was measured at 405 nm using a mini ELISA Plate Reader™ (Biolegend, 423555) (remove the blank control at 650 nm). ROC curves were plotted using the results of a sample of volunteers

(4) Protocol for the quantification of mouse fecal IgM, IgG, and IgA utilizing standard curve-based enzyme-linked immunosorbent assaysSTAR ProtocolsDecember 1, 2025KimAnh T. Pioli, Peter D. Piolialgorithms,Software and algorithms,Software and algorithms ,, Mini ELISA Plate Reader App,BioLegend,Cat# 423555; https://www.biolegend.com/nl-be/products/mini-elisa-plate-reader-20477 Microsoft Excel,Microsoft,RRID:

(5) B cell maturation antigen (BCMA) is dispensable for the survival of long-lived plasma cellsNat CommunAugust 2, 2025Shannon R. Menzel, Edith Roth, Jens Wittner, Stefanie Brey, Leonie Weckwerth, Jana Thomas, Thomas H. Winkler, Wolfgang Schuh, Hans-Martin Jäck, Katharina Pracht, et al.following the manufacturer’s protocol. ELISA plates were measured and analyzed using the Biolegend Mini ELISA plate reader at 450 nm. Analysis was performed using the “Four Parameter Logistic Curve” online data analysis tool,

(6) An Open-Source and Highly Adaptable Rodent Limited Bedding and Nesting Apparatus for Chronic Early Life StresseNeuroJune 19, 2025Olivia S. O'Neill, Dylan J. Terstege, Amisha K. Gill, Moriah Edge-Partington, Raksha Ramkumar, Jonathan R. Epp, Derya Sargin(Arbor Assays, DetectX Corticosterone Kit, K014). Absorbance at 450 nm was measured using the BioLegend Mini ELISA plate reader (BioLegend). At PND 10, pups were sacrificed for blood serum collection. Trunk blood samples were stored

(7) An Open-Source and Highly Adaptable Rodent Limited Bedding and Nesting Apparatus for Chronic Early Life StresseNeuroJune 19, 2025Olivia S. O’Neill, Dylan J. Terstege, Amisha K. Gill, Moriah Edge-Partington, Raksha Ramkumar, Jonathan R. Epp, Derya Sargin(Arbor Assays, DetectX Corticosterone Kit, K014). Absorbance at 450 nm was measured using the BioLegend Mini ELISA plate reader (BioLegend). At PND 10, pups were sacrificed for blood serum collection. Trunk blood samples were stored

(8) Asymmetric Dimethylarginine Disrupts Tumor Antigen Presentation in Breast CancerInt J Mol SciMay 8, 2025Mei Li, Yi-Ling Chen, Lilly M. Pearce, Amy M. Hammett, Falak H. Sharma, Derick S. Miller, Kuan-Hui E. Chenanalyzed via ELISA. Absorbance readings at 450 nm and 620 nm were measured using a microplate reader (423555, Biolegend). All experiments were conducted in triplicate. 4.7. Cell Proliferation Measurement The

(9) Lymphopoiesis is attenuated upon hepatocyte-specific deletion of the cytochrome c oxidase assembly factor Sco1iScienceApril 18, 2025KimAnh T. Pioli, Sampurna Ghosh, Aren Boulet, Scot C. Leary, Peter D. Piolidensities (ODs) were read at 450 nm wavelength using a BioLegend Mini ELISA Plate Reader (BioLegend, Cat# 423555). Samples and standards were analyzed following subtraction of blank wells (1x PBS) and assayed in triplicate.

(10) Decoding plasma cell maturation dynamics with BCMAFront ImmunolMarch 11, 2025Sebastian R. Schulz, Shannon R. Menzel, Jens Wittner, Carolin Ulbricht, Alina T. Grofe, Edith Roth, Ritu Mann-Nüttel, Stefanie Scheu, Andrew J. Kueh, Alexander Jäck, et al.following the manufacturer’s protocol. ELISA plates were measured and analyzed using the BioLegend Mini ELISA plate reader at 450 nm. Analysis was performed using the “Four Parameter Logistic Curve” online data analysis tool,

(11) Conserved allomorphs of MR1 drive the specificity of MR1-restricted TCRsFrontiers in OncologyOctober 3, 2024Terri V. Cornforth, Nathifa Moyo, Suzanne Cole, Emily P. S. Lam, Tatiana Lobry, Ron Wolchinsky, Angharad Lloyd, Katarzyna Ward, Eleanor M. Denham, Giulia Masi, et al.Minneapolis, MN, USA). All ELISAs were performed as per the manufacturer’s instructions. ELISAs were read on a Mini ELISA plate reader from BioLegend. ELISpot assay IFN-γ ELISpot assays were performed using the Human IFN-γ (ALP) kit

(12) A Pilot Study to Investigate Peripheral Low-Level Chronic LPS Injection as a Model of Neutrophil Activation in the Periphery and Brain in MiceInternational journal of molecular sciencesMay 14, 2024Aries M, Cook M, Hensley-McBain T.using a pre-validated kit following manufacturer’s instructions (Abcam, Cambridge, UK) and read using a Mini ELISA Plate Reader (Biolegend, San Diego, CA, USA). 4.7. Immunohistochemistry Formalin-fixed paraffin-embedded sections

(13) The cutaneous beta human papillomavirus type 8 E6 protein induces CCL2 through the CEBPα/miR-203/p63 pathway to support an inflammatory microenvironment in epidermodysplasia verruciformis skin lesionsFront Cell Infect MicrobiolMarch 6, 2024Luca Vella, Anna Sternjakob, Stefan Lohse, Alina Fingerle, Tanya Sperling, Claudia Wickenhauser, Michael Stöckle, Thomas Vogt, Klaus Roemer, Monika Ołdak, et al.ELISA MAX Deluxe Set Human MCP-1/CCL2 (BioLegend) according to the manufacturer’s specifications and the Mini ELISA Plate Reader (BioLegend). Multiplex immunoassays were performed using the Bio-Plex Pro Human Cytokine Screening Panel (48-Plex

(14) Magnitude of venous or capillary blood-derived SARS-CoV-2-specific T cell response determines COVID-19 immunityNature communicationsSeptember 21, 2022Scurr MJ, Lippiatt G, Capitani L, Bentley K, Lauder SN, Smart K, Somerville MS, Rees T, Stanton RJ, Gallimore A, et al.following the addition of stop solution (2 N H2SO4), microplates were read at 450 nm using the BioLegend Mini ELISA Plate Reader. IFN-γ was quantified by extrapolating from the standard curve using GraphPad Prism. Values below the

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